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The map label for this gene is rsmE [H]

Identifier: 226950382

GI number: 226950382

Start: 3382311

End: 3383069

Strand: Reverse

Name: rsmE [H]

Synonym: CLM_3351

Alternate gene names: 226950382

Gene position: 3383069-3382311 (Counterclockwise)

Preceding gene: 226950383

Following gene: 226950381

Centisome position: 81.42

GC content: 26.75

Gene sequence:

>759_bases
GTGCATAAATTTTTTGTACCCGCTGAAAATATAATTGATGAAAAAGCTATAATTGATGGTGATGATGTTAAACATATATA
TAAGGTATTAAGGTTAGAATGTGGAGATGCTATAAATATAAATAATTGCCAGGGAAAAGAATATAAAGGTGAAATAGAAA
GCATAGATAAAAAATCTGTTGTTGTAAAAATATTAGAACAAGTTGAAGTAAATAATGAAAACCCTTTAAATATTTATTTA
TACCAAGGTCTTCCTAAATCTACTAAAATGGATTTAATAGTTCAAAAATCTACCGAATTAGGTATAAAAGAAATAATACC
TATTATAACAGAAAGAGTTGAGGTTAAGGCTTCATTAAAGGAATTTAAAAAATTAGATAGATGGAATAGAATTGCACTTG
AAGCATGTAAACAATGTAAAAGATCTTTAATTCCAAGTGTTAGAGAACCTCTAAAATTTAATGATTTGTTGGAAGAAATA
AAGGGGATGGATTTAATAGTTGTGCCTTATGAAAGCAAGGAAAATTACGGTATAAAGTCTCTTATAAAAAATATAGAATA
TAAAAAAGAAAATATTAAAAATATAGGTATAATAATAGGACCAGAAGGTGGCTTTGAAGAAAGTGAAATAAGAGCTTTAG
AAGAAATAAAAGCTCAAATAGTAACTTTAGGGCCTAGAATATTTAGAACAGAAACAGCAGGTATAGTATGTGCTTCAATA
ATTTCATATGAATTAGGAGATCTAGGAGGAAATATATAA

Upstream 100 bases:

>100_bases
TAAAATCATTTTAATAATTTTATAAAAAGTGTATTTTTAGAATATAAAAATTTATATTTAGGACTCCCTTTATTAAAAAA
TGTTAAAGTAGGTGATTATT

Downstream 100 bases:

>100_bases
TGAAGGTTGCTTTTTCTACATTAGGATGTAGAGTAAATGTATATGAAACAGAAGCTATGACAGAAAAATTTATAAATCAA
GGTTATGAAATAGTGGATTT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MHKFFVPAENIIDEKAIIDGDDVKHIYKVLRLECGDAININNCQGKEYKGEIESIDKKSVVVKILEQVEVNNENPLNIYL
YQGLPKSTKMDLIVQKSTELGIKEIIPIITERVEVKASLKEFKKLDRWNRIALEACKQCKRSLIPSVREPLKFNDLLEEI
KGMDLIVVPYESKENYGIKSLIKNIEYKKENIKNIGIIIGPEGGFEESEIRALEEIKAQIVTLGPRIFRTETAGIVCASI
ISYELGDLGGNI

Sequences:

>Translated_252_residues
MHKFFVPAENIIDEKAIIDGDDVKHIYKVLRLECGDAININNCQGKEYKGEIESIDKKSVVVKILEQVEVNNENPLNIYL
YQGLPKSTKMDLIVQKSTELGIKEIIPIITERVEVKASLKEFKKLDRWNRIALEACKQCKRSLIPSVREPLKFNDLLEEI
KGMDLIVVPYESKENYGIKSLIKNIEYKKENIKNIGIIIGPEGGFEESEIRALEEIKAQIVTLGPRIFRTETAGIVCASI
ISYELGDLGGNI
>Mature_252_residues
MHKFFVPAENIIDEKAIIDGDDVKHIYKVLRLECGDAININNCQGKEYKGEIESIDKKSVVVKILEQVEVNNENPLNIYL
YQGLPKSTKMDLIVQKSTELGIKEIIPIITERVEVKASLKEFKKLDRWNRIALEACKQCKRSLIPSVREPLKFNDLLEEI
KGMDLIVVPYESKENYGIKSLIKNIEYKKENIKNIGIIIGPEGGFEESEIRALEEIKAQIVTLGPRIFRTETAGIVCASI
ISYELGDLGGNI

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=231, Percent_Identity=34.1991341991342, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28547; Mature: 28547

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKFFVPAENIIDEKAIIDGDDVKHIYKVLRLECGDAININNCQGKEYKGEIESIDKKSV
CCCEECCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHH
VVKILEQVEVNNENPLNIYLYQGLPKSTKMDLIVQKSTELGIKEIIPIITERVEVKASLK
HHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHHHH
EFKKLDRWNRIALEACKQCKRSLIPSVREPLKFNDLLEEIKGMDLIVVPYESKENYGIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHH
LIKNIEYKKENIKNIGIIIGPEGGFEESEIRALEEIKAQIVTLGPRIFRTETAGIVCASI
HHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHH
ISYELGDLGGNI
HHHHHHHCCCCC
>Mature Secondary Structure
MHKFFVPAENIIDEKAIIDGDDVKHIYKVLRLECGDAININNCQGKEYKGEIESIDKKSV
CCCEECCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHH
VVKILEQVEVNNENPLNIYLYQGLPKSTKMDLIVQKSTELGIKEIIPIITERVEVKASLK
HHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHHHH
EFKKLDRWNRIALEACKQCKRSLIPSVREPLKFNDLLEEIKGMDLIVVPYESKENYGIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHH
LIKNIEYKKENIKNIGIIIGPEGGFEESEIRALEEIKAQIVTLGPRIFRTETAGIVCASI
HHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHH
ISYELGDLGGNI
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]