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The map label for this gene is dnaG [H]

Identifier: 226950365

GI number: 226950365

Start: 3363909

End: 3365681

Strand: Reverse

Name: dnaG [H]

Synonym: CLM_3333

Alternate gene names: 226950365

Gene position: 3365681-3363909 (Counterclockwise)

Preceding gene: 226950366

Following gene: 226950364

Centisome position: 81.0

GC content: 27.58

Gene sequence:

>1773_bases
TTGATATCGGAAGATGTCGTCCAAAAGGTAATAGAGCTAAACGATATTGTAGATGTTATATCAGGGGACATAAAGCTTAA
AAACAGTGGAAGAAACTATTTTGGGTTATGTCCTTTTCATCATGAAAAAACACCTTCCTTTAGTGTGTCGCAGGATAAGC
AGATATATAAATGTTTTGGATGCGGAGAAGCAGGAAACGTTGTGACTTATGTAATGAAGACTAAAAACGTTGCTTTTCCA
GAGGCCATAAAAATTTTAGCAGAAAGAGTAAATATAGATATAGAGGAAGATAAAAAAGAAAATACAAATAATCCTAAAGA
TAAAATCTATAAAATAAATGTAGACGCAGCTAGATATTTTTTTAATAATCTTATAATAAATAAAAATGCTATAAATTACT
TTTTAAATAGGGGTGTTACAAAGTCAATAATTAAAAGGTTTGGATTAGGATATTCAAAGGATAGCTGGGATGGGCTTTTA
AGGCATTTAAAAACAAAAGGATATACGGAACTAGATATGCTATCTGCAGGTCTTATTATAAGGAGTAAAAATGGATCTTA
TTATGATAGATTTAGAAACAGAGTTATGTTTCCAGTCTTTGATTATAGAGGAAAGGTAATAGGCTTTGGAGGAAGGGTAC
TAAATAACGCTAAACCTAAATATTTAAACTCACCAGAAACTATGGTGTTTAAAAAGGGTATAAATTTATATGGATTAAAT
TATGCAGTAAAAGAAAATAAAGATAGAGTATTAATAATAGTAGAAGGATATATGGATTGCATTACATTACATCAATATGG
CATAAAAAATTCTGTAGCATCTCTAGGAACAGCTCTAACTACAAATCAAGCTAGACTTTTAAAACGATATGCAGATAAAG
TCATAATATCTTATGACTCAGATACAGCAGGACAATTAGCGACTCAAAGGGGCTTAGAAATATTAAAAGATGTAGGATTA
GGTGTAGAAATATTAACTGTACCAGATGGGAAAGATCCAGATCAATTTGTAAAGGCTCATGGAAAAGAAGCTTACCTAAA
GTTAGTAAAAGAAGCTTTACCTCTTATAGAGTATAAAATAAAAATTAGTAAGCAGGATTTAAATATTGGGAATAAAAGGC
AAGCTATACAATATATAGATAGATATATTAAAATTATAGAAAATTTAGATCCTGTAGAAAAAGATATGTACATAAGAAGA
CTTTCAGAGGAAACTAATATAGAAATTCAGACTTTATATGACCAATTAAACAAAAAAAACCAAAATTCTAGGAAAAATGG
AAAAGAAGTGAATATGTTAGAAGCTTTTGGACAAAAATTGTATCTAGAGCCAGCTTATATAAGAGCTGAGAGATTTATCC
TGCAATTAATGTTTAATTATAATAATATTTATGAATATATAAAAAATAGTATAAATGAGCAGGATATAATAGAGGAAACA
CATAAGGCAATATATAAATATATAAAAGATACTTTAGAAGACTCAAATATACAAAATAAAAAATTACACATAGAAACTCT
ATGTAATAAGAATGTAGAAACATCTAAAGAATGGGTAAATATATTAAATACCAATTTTATTTATAAAGAAGATGAAAGTA
AAAAATTTATAGATGATGCTATTTTAAATATTAAAAAATATAAATTAGAAAAATATAAGGAAAATTTACTTAAAAAGATT
AAAGAGTATGAAGCACAAGGAAAATTAGATGAAACTATAAAAATGAGCCAGGAACTCATCAAAGTAAAGAAAACCTTAGG
GGCCATGAAATAA

Upstream 100 bases:

>100_bases
AGAAGGAAATATAAAGTTTTTGTCGAATATATTATTTTTACCTATGTATTTTTAAAATAATATATTCATAAAATATTAAA
GAGTAAGGTGGGAGTAGTCA

Downstream 100 bases:

>100_bases
TGGCGAAAGGAGGCTACACTTATAATGAAAGAAAAGAAGAATAATGCTAAAGATAAAAATGAAAAAATGAAGTTAGTTAG
ACGCTTAATAGATAAAGGTA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MISEDVVQKVIELNDIVDVISGDIKLKNSGRNYFGLCPFHHEKTPSFSVSQDKQIYKCFGCGEAGNVVTYVMKTKNVAFP
EAIKILAERVNIDIEEDKKENTNNPKDKIYKINVDAARYFFNNLIINKNAINYFLNRGVTKSIIKRFGLGYSKDSWDGLL
RHLKTKGYTELDMLSAGLIIRSKNGSYYDRFRNRVMFPVFDYRGKVIGFGGRVLNNAKPKYLNSPETMVFKKGINLYGLN
YAVKENKDRVLIIVEGYMDCITLHQYGIKNSVASLGTALTTNQARLLKRYADKVIISYDSDTAGQLATQRGLEILKDVGL
GVEILTVPDGKDPDQFVKAHGKEAYLKLVKEALPLIEYKIKISKQDLNIGNKRQAIQYIDRYIKIIENLDPVEKDMYIRR
LSEETNIEIQTLYDQLNKKNQNSRKNGKEVNMLEAFGQKLYLEPAYIRAERFILQLMFNYNNIYEYIKNSINEQDIIEET
HKAIYKYIKDTLEDSNIQNKKLHIETLCNKNVETSKEWVNILNTNFIYKEDESKKFIDDAILNIKKYKLEKYKENLLKKI
KEYEAQGKLDETIKMSQELIKVKKTLGAMK

Sequences:

>Translated_590_residues
MISEDVVQKVIELNDIVDVISGDIKLKNSGRNYFGLCPFHHEKTPSFSVSQDKQIYKCFGCGEAGNVVTYVMKTKNVAFP
EAIKILAERVNIDIEEDKKENTNNPKDKIYKINVDAARYFFNNLIINKNAINYFLNRGVTKSIIKRFGLGYSKDSWDGLL
RHLKTKGYTELDMLSAGLIIRSKNGSYYDRFRNRVMFPVFDYRGKVIGFGGRVLNNAKPKYLNSPETMVFKKGINLYGLN
YAVKENKDRVLIIVEGYMDCITLHQYGIKNSVASLGTALTTNQARLLKRYADKVIISYDSDTAGQLATQRGLEILKDVGL
GVEILTVPDGKDPDQFVKAHGKEAYLKLVKEALPLIEYKIKISKQDLNIGNKRQAIQYIDRYIKIIENLDPVEKDMYIRR
LSEETNIEIQTLYDQLNKKNQNSRKNGKEVNMLEAFGQKLYLEPAYIRAERFILQLMFNYNNIYEYIKNSINEQDIIEET
HKAIYKYIKDTLEDSNIQNKKLHIETLCNKNVETSKEWVNILNTNFIYKEDESKKFIDDAILNIKKYKLEKYKENLLKKI
KEYEAQGKLDETIKMSQELIKVKKTLGAMK
>Mature_590_residues
MISEDVVQKVIELNDIVDVISGDIKLKNSGRNYFGLCPFHHEKTPSFSVSQDKQIYKCFGCGEAGNVVTYVMKTKNVAFP
EAIKILAERVNIDIEEDKKENTNNPKDKIYKINVDAARYFFNNLIINKNAINYFLNRGVTKSIIKRFGLGYSKDSWDGLL
RHLKTKGYTELDMLSAGLIIRSKNGSYYDRFRNRVMFPVFDYRGKVIGFGGRVLNNAKPKYLNSPETMVFKKGINLYGLN
YAVKENKDRVLIIVEGYMDCITLHQYGIKNSVASLGTALTTNQARLLKRYADKVIISYDSDTAGQLATQRGLEILKDVGL
GVEILTVPDGKDPDQFVKAHGKEAYLKLVKEALPLIEYKIKISKQDLNIGNKRQAIQYIDRYIKIIENLDPVEKDMYIRR
LSEETNIEIQTLYDQLNKKNQNSRKNGKEVNMLEAFGQKLYLEPAYIRAERFILQLMFNYNNIYEYIKNSINEQDIIEET
HKAIYKYIKDTLEDSNIQNKKLHIETLCNKNVETSKEWVNILNTNFIYKEDESKKFIDDAILNIKKYKLEKYKENLLKKI
KEYEAQGKLDETIKMSQELIKVKKTLGAMK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=369, Percent_Identity=39.2953929539295, Blast_Score=289, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68277; Mature: 68277

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISEDVVQKVIELNDIVDVISGDIKLKNSGRNYFGLCPFHHEKTPSFSVSQDKQIYKCFG
CCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCC
CGEAGNVVTYVMKTKNVAFPEAIKILAERVNIDIEEDKKENTNNPKDKIYKINVDAARYF
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHH
FNNLIINKNAINYFLNRGVTKSIIKRFGLGYSKDSWDGLLRHLKTKGYTELDMLSAGLII
HHHHEECCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCEEE
RSKNGSYYDRFRNRVMFPVFDYRGKVIGFGGRVLNNAKPKYLNSPETMVFKKGINLYGLN
ECCCCCHHHHHHCCEEEEEECCCCCEEEECCHHHCCCCCCCCCCCCHHHHHCCCCEEECE
YAVKENKDRVLIIVEGYMDCITLHQYGIKNSVASLGTALTTNQARLLKRYADKVIISYDS
EEEECCCCEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECC
DTAGQLATQRGLEILKDVGLGVEILTVPDGKDPDQFVKAHGKEAYLKLVKEALPLIEYKI
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHEEEEE
KISKQDLNIGNKRQAIQYIDRYIKIIENLDPVEKDMYIRRLSEETNIEIQTLYDQLNKKN
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
QNSRKNGKEVNMLEAFGQKLYLEPAYIRAERFILQLMFNYNNIYEYIKNSINEQDIIEET
CCCCCCCCCCHHHHHHCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HKAIYKYIKDTLEDSNIQNKKLHIETLCNKNVETSKEWVNILNTNFIYKEDESKKFIDDA
HHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHH
ILNIKKYKLEKYKENLLKKIKEYEAQGKLDETIKMSQELIKVKKTLGAMK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISEDVVQKVIELNDIVDVISGDIKLKNSGRNYFGLCPFHHEKTPSFSVSQDKQIYKCFG
CCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCC
CGEAGNVVTYVMKTKNVAFPEAIKILAERVNIDIEEDKKENTNNPKDKIYKINVDAARYF
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHH
FNNLIINKNAINYFLNRGVTKSIIKRFGLGYSKDSWDGLLRHLKTKGYTELDMLSAGLII
HHHHEECCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCEEE
RSKNGSYYDRFRNRVMFPVFDYRGKVIGFGGRVLNNAKPKYLNSPETMVFKKGINLYGLN
ECCCCCHHHHHHCCEEEEEECCCCCEEEECCHHHCCCCCCCCCCCCHHHHHCCCCEEECE
YAVKENKDRVLIIVEGYMDCITLHQYGIKNSVASLGTALTTNQARLLKRYADKVIISYDS
EEEECCCCEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECC
DTAGQLATQRGLEILKDVGLGVEILTVPDGKDPDQFVKAHGKEAYLKLVKEALPLIEYKI
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHEEEEE
KISKQDLNIGNKRQAIQYIDRYIKIIENLDPVEKDMYIRRLSEETNIEIQTLYDQLNKKN
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
QNSRKNGKEVNMLEAFGQKLYLEPAYIRAERFILQLMFNYNNIYEYIKNSINEQDIIEET
CCCCCCCCCCHHHHHHCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HKAIYKYIKDTLEDSNIQNKKLHIETLCNKNVETSKEWVNILNTNFIYKEDESKKFIDDA
HHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHH
ILNIKKYKLEKYKENLLKKIKEYEAQGKLDETIKMSQELIKVKKTLGAMK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]