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The map label for this gene is yaaJ [C]

Identifier: 226950333

GI number: 226950333

Start: 3328212

End: 3329564

Strand: Reverse

Name: yaaJ [C]

Synonym: CLM_3299

Alternate gene names: 226950333

Gene position: 3329564-3328212 (Counterclockwise)

Preceding gene: 226950334

Following gene: 226950332

Centisome position: 80.13

GC content: 32.67

Gene sequence:

>1353_bases
ATGGATCTTTTAAATGCTGTGAAAGGCATAAACAATGTACTATGGAATTATGTATTGATTTTTTTGTTGTGTGGAACAGG
TGTTATGTTTACTGTATCTTTGAAATTTGTTCAAGTTTCTAAATTCAAAGAATCCTTTAAAAAAGCCTTTGGAGGAATGA
GTTTAAAAGGAAAAAAAGCAGGTAAAGATGGAATGAGCTCATTTCAATCATTAGCAACAGCAGTAGCGGCTCAAGTAGGT
ACAGGTAATTTAGCAGGAGCAGCAACAGCTATAGTATCAGGGGGACCTGGAGCAATTTTTTGGATGTGGATTAGTGCATT
TTTAGGAATGGCAACTATTTTTGGAGAAGCGGTTTTAGCACAGATTTTTAAAGAAAAAGTTAATGGAGAAGTTACAGGTG
GTCCAGCCTATTATATAAGTAAAGGACTGAAAAGTAACTTTTTAGCTAGTTTTTTTTCTGTAACTATAATATTAGCCCTA
GGATTTATAGGTAATATGGTTCAGGCCAATTCTATAGGGGCAGCCTTTTCAAATATTTCTAATATACCATCTTGGATAAT
AGGTATAATGATTGCAATACTTGGATTAGTTGTTTTTGTGGGGGGAATAGGAAGAATCGCTTCTGTAACAGAAAAAATGG
TTCCTATAATGGCGGCGTTTTATATTATAGGAAGTATTATAATATTAGCTAAGAATTATACAAATATATTACCTGCTTTT
AAATTAATATTTGTCTCTGCTTTTAATCCTAAGGCTGCTGTAGGGGGAGTAGCAGGTGTTACTGTAAAACAGGCTATACG
ATATGGAGTAGCAAGAGGATTATTTTCCAATGAAGCTGGAATGGGATCAACACCACATGCTCATGCGGTAGCCAAGGTAA
AACATCCAGTAGAACAAGGATTAGTAGCTATAGTTGGGGTATTTATAGATACTTTTATAGTTCTTACATTTACAGCACTA
GTAATACTTGCGACAGGTGCATTAGATGGAAAGACAACAGGCATAGAATTAACACAAAATGCATTTACTCAGGGATTAGG
TAATTTTGGTGGATATTTTATAGCTATTGCATTATTTTTCTTTGCTTTTTCAACAATAATTGGATGGTATTTCTTTGGAG
AAGCTAATGTAAAATATCTCTTTAAAGGCAAGGGATTAAATATATATAGATTGTTAGTGGCAATTTTTATAGTTGTAGGA
ACAACTTTAAAAGTAGACTTAGTTTGGGAGCTTGCAGATACTTTTAATGGACTTATGGTAATTCCTAATGTAATAGCATT
GATAGCTTTAGCTAAAATAGTAAAAGAGTCATTAAAGGATTATAATGAAAATTTTAAAATTAAAGATATATAA

Upstream 100 bases:

>100_bases
GGAGGCCTTTTTATGTATTATTTTTATACATTAAAGGTCTTTTTTAGTTTTAGTTATTTCTTTTGTCCTCATTATTCAAT
TAGAAAAAGGAGGAATATGG

Downstream 100 bases:

>100_bases
TACTTTAATTGGATTTTTAAAGTATAAATAATACAATGATAAAAGGGATACAATAGAATAAATAAAAGTTTAATTCAGAT
GGAGATTTACTCTATCTGAA

Product: sodium:alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDLLNAVKGINNVLWNYVLIFLLCGTGVMFTVSLKFVQVSKFKESFKKAFGGMSLKGKKAGKDGMSSFQSLATAVAAQVG
TGNLAGAATAIVSGGPGAIFWMWISAFLGMATIFGEAVLAQIFKEKVNGEVTGGPAYYISKGLKSNFLASFFSVTIILAL
GFIGNMVQANSIGAAFSNISNIPSWIIGIMIAILGLVVFVGGIGRIASVTEKMVPIMAAFYIIGSIIILAKNYTNILPAF
KLIFVSAFNPKAAVGGVAGVTVKQAIRYGVARGLFSNEAGMGSTPHAHAVAKVKHPVEQGLVAIVGVFIDTFIVLTFTAL
VILATGALDGKTTGIELTQNAFTQGLGNFGGYFIAIALFFFAFSTIIGWYFFGEANVKYLFKGKGLNIYRLLVAIFIVVG
TTLKVDLVWELADTFNGLMVIPNVIALIALAKIVKESLKDYNENFKIKDI

Sequences:

>Translated_450_residues
MDLLNAVKGINNVLWNYVLIFLLCGTGVMFTVSLKFVQVSKFKESFKKAFGGMSLKGKKAGKDGMSSFQSLATAVAAQVG
TGNLAGAATAIVSGGPGAIFWMWISAFLGMATIFGEAVLAQIFKEKVNGEVTGGPAYYISKGLKSNFLASFFSVTIILAL
GFIGNMVQANSIGAAFSNISNIPSWIIGIMIAILGLVVFVGGIGRIASVTEKMVPIMAAFYIIGSIIILAKNYTNILPAF
KLIFVSAFNPKAAVGGVAGVTVKQAIRYGVARGLFSNEAGMGSTPHAHAVAKVKHPVEQGLVAIVGVFIDTFIVLTFTAL
VILATGALDGKTTGIELTQNAFTQGLGNFGGYFIAIALFFFAFSTIIGWYFFGEANVKYLFKGKGLNIYRLLVAIFIVVG
TTLKVDLVWELADTFNGLMVIPNVIALIALAKIVKESLKDYNENFKIKDI
>Mature_450_residues
MDLLNAVKGINNVLWNYVLIFLLCGTGVMFTVSLKFVQVSKFKESFKKAFGGMSLKGKKAGKDGMSSFQSLATAVAAQVG
TGNLAGAATAIVSGGPGAIFWMWISAFLGMATIFGEAVLAQIFKEKVNGEVTGGPAYYISKGLKSNFLASFFSVTIILAL
GFIGNMVQANSIGAAFSNISNIPSWIIGIMIAILGLVVFVGGIGRIASVTEKMVPIMAAFYIIGSIIILAKNYTNILPAF
KLIFVSAFNPKAAVGGVAGVTVKQAIRYGVARGLFSNEAGMGSTPHAHAVAKVKHPVEQGLVAIVGVFIDTFIVLTFTAL
VILATGALDGKTTGIELTQNAFTQGLGNFGGYFIAIALFFFAFSTIIGWYFFGEANVKYLFKGKGLNIYRLLVAIFIVVG
TTLKVDLVWELADTFNGLMVIPNVIALIALAKIVKESLKDYNENFKIKDI

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=448, Percent_Identity=39.9553571428571, Blast_Score=294, Evalue=9e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 47788; Mature: 47788

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHCCCCCEEECC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]