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The map label for this gene is ydeM [C]

Identifier: 226950290

GI number: 226950290

Start: 3271746

End: 3273179

Strand: Reverse

Name: ydeM [C]

Synonym: CLM_3252

Alternate gene names: 226950290

Gene position: 3273179-3271746 (Counterclockwise)

Preceding gene: 226950291

Following gene: 226950289

Centisome position: 78.77

GC content: 26.85

Gene sequence:

>1434_bases
ATGAATGTAGAAGGTTCTTTTATTCATCTATTTAAAACTAGAGGAGGCAACTATGCTTATGATGTAAATACAAATAATTT
CTTAAAAATTAGTAAGGATACTTATGAATTATTATGTAAAAATATGAATTCAGATAGTAAAGAAAAACAGCTTGAAGTTA
TTGAAGATATAAAAAAAGAAGGATTTCTATCTTCTAATACAATAGAAGAAATGATTCATCCAATGAGCGAGGTATTGCCA
AGTTACTTAGATACTTCTCTTTCTATGCTTACACTTCAAGTTACTCAACAATGTAATTTTAGATGTAAATATTGCACATA
TTCAGGACTTGATGAAAATAGAACACATTCAGACAAAGATATGAGTTTAGATATAGCCAAAAAAGCTATAGACTTTTATA
TCAAGCATTCTAGGGATTCTAAATACTTAAATATTGGATTTTATGGGGGAGAGCCTCTTTTAAAGTTTGATTTTGTAAAG
GAATGTGTTAATTATGCCAAGGAAAAAGGGGAAGGTAAAACCGTAGAATTTAACATGACTACTAATGGAACTTTATTTAA
TGAAGAAAGATTAAAATTTTTAAGTGAAAATAACTTTAGAATAATGATTAGTCTTGATGGACCAAAAGAAATACATGATA
AAAATAGAACATTTTTAAATGGAGAAGGAACTTTTGATAAGATAATGAAAAATATTGAGATGATAAAATATAAGTATCCT
AAATTTTTAGACGAAAATATTGATTTTAATGCAGTTATAGATGGGAAAAGTGATGTAGTATGTACTACTAAGTTTTTTAT
TAATTATGATACAGTAAAAGATTTAAAAGTAAGAACCTCCTTTGTTAGTGATACATACAAGAAAATTGAACAATTTACAG
TGGAAGAAGAGAATATGATAAAAATCGGTTATGAAGGATTTAAAGTATTGTTATGGAAACTTGGTAAGTTAGATGAAAAG
CATGTATCAAAACTTTTTCAAGGTAACTTTAACAATGTTAAAAATCTTTTACATGATAAAAAATATACCTATAAGAAACT
AAACAAAAAGGGGCATCATGGGGGACCATGTATTCCAGGAGTTACAAGATTATTTGTAACCGTAGATGGAAAATTTTTTC
CTTGTGAAAAGGTAAATGAAAAATCTAAAGTAATGCAAATTGGAAATATAAGTGATGGATTTGATATTGAAAAAATAAGA
AATCTTATGAATGTAGGTAAGCTTACAGAAAATAAATGCAAAAAGTGTTGGGCATTTAGATATTGTTTCTTATGTTCAGC
ATTTGCAGATGACAATACTAAGTTATCAAAAGAAAAGAAAACTTCAAGATGTACAGAGGTTAGAGCTTCTGTAGATGACA
TGTTTAAAACTTATTGTACTTTAAAAGAGTTTGGATATGATTTTGAAGAAAAAAGAATTATGGAAAATGTATAA

Upstream 100 bases:

>100_bases
AGGCAAATTCAGCTAATATGTCCAAATGGGAATAGTATCCAATAAAGATGTAAGAGAATATTCTCTTACATCTTTACTAA
AAAATTTAGGAGGCAATATT

Downstream 100 bases:

>100_bases
ATAATATGTTTGAATAAAGGAGAATTTTACATGAGCATTAACAAAGAAAAAGCAATGATATACCCTTTTGATAGCGAATT
TGCACCTATAATAAGACATC

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MNVEGSFIHLFKTRGGNYAYDVNTNNFLKISKDTYELLCKNMNSDSKEKQLEVIEDIKKEGFLSSNTIEEMIHPMSEVLP
SYLDTSLSMLTLQVTQQCNFRCKYCTYSGLDENRTHSDKDMSLDIAKKAIDFYIKHSRDSKYLNIGFYGGEPLLKFDFVK
ECVNYAKEKGEGKTVEFNMTTNGTLFNEERLKFLSENNFRIMISLDGPKEIHDKNRTFLNGEGTFDKIMKNIEMIKYKYP
KFLDENIDFNAVIDGKSDVVCTTKFFINYDTVKDLKVRTSFVSDTYKKIEQFTVEEENMIKIGYEGFKVLLWKLGKLDEK
HVSKLFQGNFNNVKNLLHDKKYTYKKLNKKGHHGGPCIPGVTRLFVTVDGKFFPCEKVNEKSKVMQIGNISDGFDIEKIR
NLMNVGKLTENKCKKCWAFRYCFLCSAFADDNTKLSKEKKTSRCTEVRASVDDMFKTYCTLKEFGYDFEEKRIMENV

Sequences:

>Translated_477_residues
MNVEGSFIHLFKTRGGNYAYDVNTNNFLKISKDTYELLCKNMNSDSKEKQLEVIEDIKKEGFLSSNTIEEMIHPMSEVLP
SYLDTSLSMLTLQVTQQCNFRCKYCTYSGLDENRTHSDKDMSLDIAKKAIDFYIKHSRDSKYLNIGFYGGEPLLKFDFVK
ECVNYAKEKGEGKTVEFNMTTNGTLFNEERLKFLSENNFRIMISLDGPKEIHDKNRTFLNGEGTFDKIMKNIEMIKYKYP
KFLDENIDFNAVIDGKSDVVCTTKFFINYDTVKDLKVRTSFVSDTYKKIEQFTVEEENMIKIGYEGFKVLLWKLGKLDEK
HVSKLFQGNFNNVKNLLHDKKYTYKKLNKKGHHGGPCIPGVTRLFVTVDGKFFPCEKVNEKSKVMQIGNISDGFDIEKIR
NLMNVGKLTENKCKKCWAFRYCFLCSAFADDNTKLSKEKKTSRCTEVRASVDDMFKTYCTLKEFGYDFEEKRIMENV
>Mature_477_residues
MNVEGSFIHLFKTRGGNYAYDVNTNNFLKISKDTYELLCKNMNSDSKEKQLEVIEDIKKEGFLSSNTIEEMIHPMSEVLP
SYLDTSLSMLTLQVTQQCNFRCKYCTYSGLDENRTHSDKDMSLDIAKKAIDFYIKHSRDSKYLNIGFYGGEPLLKFDFVK
ECVNYAKEKGEGKTVEFNMTTNGTLFNEERLKFLSENNFRIMISLDGPKEIHDKNRTFLNGEGTFDKIMKNIEMIKYKYP
KFLDENIDFNAVIDGKSDVVCTTKFFINYDTVKDLKVRTSFVSDTYKKIEQFTVEEENMIKIGYEGFKVLLWKLGKLDEK
HVSKLFQGNFNNVKNLLHDKKYTYKKLNKKGHHGGPCIPGVTRLFVTVDGKFFPCEKVNEKSKVMQIGNISDGFDIEKIR
NLMNVGKLTENKCKKCWAFRYCFLCSAFADDNTKLSKEKKTSRCTEVRASVDDMFKTYCTLKEFGYDFEEKRIMENV

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemical mechanism initiated via the reduc

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=141, Percent_Identity=37.5886524822695, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994973, Length=160, Percent_Identity=28.75, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 55462; Mature: 55462

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVEGSFIHLFKTRGGNYAYDVNTNNFLKISKDTYELLCKNMNSDSKEKQLEVIEDIKKE
CCCCCCEEEEEEECCCCEEEECCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GFLSSNTIEEMIHPMSEVLPSYLDTSLSMLTLQVTQQCNFRCKYCTYSGLDENRTHSDKD
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHEEHHCCCCEEEEEEECCCCCCCCCCCCC
MSLDIAKKAIDFYIKHSRDSKYLNIGFYGGEPLLKFDFVKECVNYAKEKGEGKTVEFNMT
CHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC
TNGTLFNEERLKFLSENNFRIMISLDGPKEIHDKNRTFLNGEGTFDKIMKNIEMIKYKYP
CCCEEECHHHHHHHCCCCEEEEEEECCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHHCC
KFLDENIDFNAVIDGKSDVVCTTKFFINYDTVKDLKVRTSFVSDTYKKIEQFTVEEENMI
HHHCCCCCEEEEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
KIGYEGFKVLLWKLGKLDEKHVSKLFQGNFNNVKNLLHDKKYTYKKLNKKGHHGGPCIPG
EECHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCC
VTRLFVTVDGKFFPCEKVNEKSKVMQIGNISDGFDIEKIRNLMNVGKLTENKCKKCWAFR
CEEEEEEECCCCCCHHHCCCHHHEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
YCFLCSAFADDNTKLSKEKKTSRCTEVRASVDDMFKTYCTLKEFGYDFEEKRIMENV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MNVEGSFIHLFKTRGGNYAYDVNTNNFLKISKDTYELLCKNMNSDSKEKQLEVIEDIKKE
CCCCCCEEEEEEECCCCEEEECCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GFLSSNTIEEMIHPMSEVLPSYLDTSLSMLTLQVTQQCNFRCKYCTYSGLDENRTHSDKD
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHEEHHCCCCEEEEEEECCCCCCCCCCCCC
MSLDIAKKAIDFYIKHSRDSKYLNIGFYGGEPLLKFDFVKECVNYAKEKGEGKTVEFNMT
CHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC
TNGTLFNEERLKFLSENNFRIMISLDGPKEIHDKNRTFLNGEGTFDKIMKNIEMIKYKYP
CCCEEECHHHHHHHCCCCEEEEEEECCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHHCC
KFLDENIDFNAVIDGKSDVVCTTKFFINYDTVKDLKVRTSFVSDTYKKIEQFTVEEENMI
HHHCCCCCEEEEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
KIGYEGFKVLLWKLGKLDEKHVSKLFQGNFNNVKNLLHDKKYTYKKLNKKGHHGGPCIPG
EECHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCC
VTRLFVTVDGKFFPCEKVNEKSKVMQIGNISDGFDIEKIRNLMNVGKLTENKCKKCWAFR
CEEEEEEECCCCCCHHHCCCHHHEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
YCFLCSAFADDNTKLSKEKKTSRCTEVRASVDDMFKTYCTLKEFGYDFEEKRIMENV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA