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The map label for this gene is ctfB [H]

Identifier: 226950262

GI number: 226950262

Start: 3243179

End: 3243835

Strand: Direct

Name: ctfB [H]

Synonym: CLM_3219

Alternate gene names: 226950262

Gene position: 3243179-3243835 (Clockwise)

Preceding gene: 226950261

Following gene: 226950270

Centisome position: 78.05

GC content: 36.83

Gene sequence:

>657_bases
ATGATGAATAGTAAAGAGATAATTGCAAGAAGAATCGCGAAAGAGTTTAAAGAGGGAATGGTTGCTAATTTAGGATTCGG
TATACCCAATATGGCTGCAAATTATTTGCCAGAAGGAATGGAAATAACACTACAATGTGAAAATGGTGCATTGAAGTTTG
GTGCAACTCCAAGTATAGGTGAAGCTGATCCTGACTTAGCTAATTCTGGTGGAGCTCCAATTACTTTATTGCCAGGAGCA
TCAACATTTGAAATGGATCTATCTTTTGCAATAATAAGAGGTGGACACGTTGATATAACTGTACTAGGCGCCTTAGAAGT
CGATCAAGAAGGAAATATTGCAAACTGGAAGATACCGGGAGTTTTTGCGCCAGGTATGGGCGGTGCAATGGACCTTTTAG
CAGGAGCAAAATATGTAATAGCTGCTTTAAGTCATAGTGATAAAAAAGGTAATCCAAAGGTGCTAAAGAAATGCACATTA
CCTTTATCAGGTAAAAAGTGCGTTGATCTTATAATTACTGATAAAGCAGTTATTAAGGTTACAGATAAAGGATTAGTATT
AATGGAAGTGGCACCAGGTGTAACTGTTGATGAAGTAGTAAAGACCACTGATGCTGATTTATTAATAGCTGATGATGTTA
AGGAAATGGAAATATAA

Upstream 100 bases:

>100_bases
TATTGGTAGATATAATAGTTCAAGGAGACTCTTTTGGAGAGAGAAAAAAATATTTTGAAGATTTATGGGTAGCAACTAAC
AAAATGAAATAGGGGGAGAA

Downstream 100 bases:

>100_bases
TATACTTATATACAAGATGAAGAATGAAAGATGAATCAATCTACTTTCGAACATGAATTTGATACCTAGGGCTTAAACCA
TAAAACCTAGGAAGGGGCTG

Product: CoA-transferase subunit B

Products: NA

Alternate protein names: Coat B; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit B [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MMNSKEIIARRIAKEFKEGMVANLGFGIPNMAANYLPEGMEITLQCENGALKFGATPSIGEADPDLANSGGAPITLLPGA
STFEMDLSFAIIRGGHVDITVLGALEVDQEGNIANWKIPGVFAPGMGGAMDLLAGAKYVIAALSHSDKKGNPKVLKKCTL
PLSGKKCVDLIITDKAVIKVTDKGLVLMEVAPGVTVDEVVKTTDADLLIADDVKEMEI

Sequences:

>Translated_218_residues
MMNSKEIIARRIAKEFKEGMVANLGFGIPNMAANYLPEGMEITLQCENGALKFGATPSIGEADPDLANSGGAPITLLPGA
STFEMDLSFAIIRGGHVDITVLGALEVDQEGNIANWKIPGVFAPGMGGAMDLLAGAKYVIAALSHSDKKGNPKVLKKCTL
PLSGKKCVDLIITDKAVIKVTDKGLVLMEVAPGVTVDEVVKTTDADLLIADDVKEMEI
>Mature_218_residues
MMNSKEIIARRIAKEFKEGMVANLGFGIPNMAANYLPEGMEITLQCENGALKFGATPSIGEADPDLANSGGAPITLLPGA
STFEMDLSFAIIRGGHVDITVLGALEVDQEGNIANWKIPGVFAPGMGGAMDLLAGAKYVIAALSHSDKKGNPKVLKKCTL
PLSGKKCVDLIITDKAVIKVTDKGLVLMEVAPGVTVDEVVKTTDADLLIADDVKEMEI

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation [H]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=215, Percent_Identity=50.2325581395349, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI11545841, Length=217, Percent_Identity=45.6221198156682, Blast_Score=200, Evalue=7e-52,
Organism=Escherichia coli, GI1788552, Length=213, Percent_Identity=47.887323943662, Blast_Score=218, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17531517, Length=216, Percent_Identity=52.3148148148148, Blast_Score=208, Evalue=2e-54,
Organism=Drosophila melanogaster, GI21358325, Length=215, Percent_Identity=52.093023255814, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24655794, Length=215, Percent_Identity=52.093023255814, Blast_Score=223, Evalue=8e-59,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.9 [H]

Molecular weight: Translated: 22896; Mature: 22896

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNSKEIIARRIAKEFKEGMVANLGFGIPNMAANYLPEGMEITLQCENGALKFGATPSIG
CCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHCCCCCEEEEEECCCEEEECCCCCCC
EADPDLANSGGAPITLLPGASTFEMDLSFAIIRGGHVDITVLGALEVDQEGNIANWKIPG
CCCCCCCCCCCCCEEEECCCCEEEEEEEEEEEECCEEEEEEEEEEEECCCCCEEEEEECC
VFAPGMGGAMDLLAGAKYVIAALSHSDKKGNPKVLKKCTLPLSGKKCVDLIITDKAVIKV
EECCCCCCHHHHHHCCHHEEEEECCCCCCCCHHHHHEECCCCCCCCEEEEEEECCEEEEE
TDKGLVLMEVAPGVTVDEVVKTTDADLLIADDVKEMEI
CCCCEEEEEECCCCCHHHHHHCCCCCEEEECCHHHHCC
>Mature Secondary Structure
MMNSKEIIARRIAKEFKEGMVANLGFGIPNMAANYLPEGMEITLQCENGALKFGATPSIG
CCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHCCCCCEEEEEECCCEEEECCCCCCC
EADPDLANSGGAPITLLPGASTFEMDLSFAIIRGGHVDITVLGALEVDQEGNIANWKIPG
CCCCCCCCCCCCCEEEECCCCEEEEEEEEEEEECCEEEEEEEEEEEECCCCCEEEEEECC
VFAPGMGGAMDLLAGAKYVIAALSHSDKKGNPKVLKKCTLPLSGKKCVDLIITDKAVIKV
EECCCCCCHHHHHHCCHHEEEEECCCCCCCCHHHHHEECCCCCCCCEEEEEEECCEEEEE
TDKGLVLMEVAPGVTVDEVVKTTDADLLIADDVKEMEI
CCCCEEEEEECCCCCHHHHHHCCCCCEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254264; 8226639; 8423010; 11466286; 2383002 [H]