Click here to switch to the map view.

The map label for this gene is glpF [H]

Identifier: 226950197

GI number: 226950197

Start: 3162407

End: 3163111

Strand: Reverse

Name: glpF [H]

Synonym: CLM_3151

Alternate gene names: 226950197

Gene position: 3163111-3162407 (Counterclockwise)

Preceding gene: 226950199

Following gene: 226950196

Centisome position: 76.12

GC content: 38.58

Gene sequence:

>705_bases
ATGTCAATTTATATGGCGGAGTTTTTGGGTACTATGATGCTAATTTGGTTAGGGGATGGCGTTGTAGCAAGTGTAGCTCT
TAACAAAAGTAAGGGAAAGGATGGAGGCTGGATTGTTGTAACAGCAGCCTGGGGATTAGCTGTTGCTATACCTGCATATA
TTTTTGGAGGTATAAGTGGAGCTCATATGAATCCAGCAGTTACAATTGGTAATGCAATTGCAGGAAATTTTCCTTGGGCA
AATGTAGCAGGTTATATTACAGCTCAAATGTTAGGCGGGTTTGCAGGAGCAGTTTTAGTTTGGCTTACTTACCTTCCACA
TTGGAAAGCAACAGAAGATAAAGTTACTAAGCTGGGAGTATTTTGTACAGCTCCAGCAATAAGAGACACAAAAGGTAACT
TTATAACTGAATTTTTAGTAACTGCTCTTTTAGTATTTGGACTAATGGGTCTTGGACAAGTTAAAATGGTAGATGGCTTT
GGACCATTAACTGCAGGTGCACTTATATTTGTTCTGGGACTTTCTTTAGGTGGACCAACAGGATATGCTATAAATCCCGC
GAGAGATTTAGCACCAAGAATAGCTCATGCAATTTTACCAATAGCGGGTAAAGGAGATTCGGATTGGAAATATGCATGGA
TACCAGTACTAGGTCCAATATTAGGAGGAATATTAGGAGCATTATTATTTATGTTAGTTTTTTAA

Upstream 100 bases:

>100_bases
GGCGGAGATAAGAGAGCCAATTCATTACGTTTCAATGGGTGAGTTGACTCTATTTTGTCTTTTCCAAAATTAAATAATTT
TAACAGGGGGAGTAAAAATT

Downstream 100 bases:

>100_bases
TCGAAACTTTATGAAAACCTTTTAAGTAAATTATTTTATCATGATATAATAAAACAAAAATAATATCGATGAGCTATATA
ATAGAAAAATATTTAAAAAT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSIYMAEFLGTMMLIWLGDGVVASVALNKSKGKDGGWIVVTAAWGLAVAIPAYIFGGISGAHMNPAVTIGNAIAGNFPWA
NVAGYITAQMLGGFAGAVLVWLTYLPHWKATEDKVTKLGVFCTAPAIRDTKGNFITEFLVTALLVFGLMGLGQVKMVDGF
GPLTAGALIFVLGLSLGGPTGYAINPARDLAPRIAHAILPIAGKGDSDWKYAWIPVLGPILGGILGALLFMLVF

Sequences:

>Translated_234_residues
MSIYMAEFLGTMMLIWLGDGVVASVALNKSKGKDGGWIVVTAAWGLAVAIPAYIFGGISGAHMNPAVTIGNAIAGNFPWA
NVAGYITAQMLGGFAGAVLVWLTYLPHWKATEDKVTKLGVFCTAPAIRDTKGNFITEFLVTALLVFGLMGLGQVKMVDGF
GPLTAGALIFVLGLSLGGPTGYAINPARDLAPRIAHAILPIAGKGDSDWKYAWIPVLGPILGGILGALLFMLVF
>Mature_233_residues
SIYMAEFLGTMMLIWLGDGVVASVALNKSKGKDGGWIVVTAAWGLAVAIPAYIFGGISGAHMNPAVTIGNAIAGNFPWAN
VAGYITAQMLGGFAGAVLVWLTYLPHWKATEDKVTKLGVFCTAPAIRDTKGNFITEFLVTALLVFGLMGLGQVKMVDGFG
PLTAGALIFVLGLSLGGPTGYAINPARDLAPRIAHAILPIAGKGDSDWKYAWIPVLGPILGGILGALLFMLVF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=244, Percent_Identity=36.0655737704918, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI4826645, Length=244, Percent_Identity=34.4262295081967, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI4502187, Length=244, Percent_Identity=33.6065573770492, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI22538420, Length=244, Percent_Identity=31.9672131147541, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI4502179, Length=232, Percent_Identity=32.7586206896552, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI310133356, Length=221, Percent_Identity=30.316742081448, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI310114181, Length=221, Percent_Identity=30.316742081448, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI4755125, Length=204, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4502181, Length=204, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1790362, Length=246, Percent_Identity=35.7723577235772, Blast_Score=114, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71994009, Length=233, Percent_Identity=32.1888412017167, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17544068, Length=242, Percent_Identity=33.4710743801653, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71992966, Length=245, Percent_Identity=28.9795918367347, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17531429, Length=241, Percent_Identity=28.6307053941909, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17531431, Length=241, Percent_Identity=28.6307053941909, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17558372, Length=237, Percent_Identity=29.1139240506329, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=237, Percent_Identity=28.2700421940928, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI32564052, Length=239, Percent_Identity=26.7782426778243, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17533613, Length=237, Percent_Identity=27.0042194092827, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71993722, Length=235, Percent_Identity=29.3617021276596, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=35.4368932038835, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322985, Length=277, Percent_Identity=28.5198555956679, Blast_Score=93, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24652747, Length=234, Percent_Identity=29.9145299145299, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI45551084, Length=234, Percent_Identity=29.9145299145299, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19922828, Length=194, Percent_Identity=30.9278350515464, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24762342, Length=243, Percent_Identity=27.1604938271605, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24762344, Length=238, Percent_Identity=29.8319327731092, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24762346, Length=238, Percent_Identity=29.8319327731092, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24762348, Length=238, Percent_Identity=29.8319327731092, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI20130305, Length=238, Percent_Identity=29.8319327731092, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24409; Mature: 24278

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYMAEFLGTMMLIWLGDGVVASVALNKSKGKDGGWIVVTAAWGLAVAIPAYIFGGISG
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCC
AHMNPAVTIGNAIAGNFPWANVAGYITAQMLGGFAGAVLVWLTYLPHWKATEDKVTKLGV
CCCCCCEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHE
FCTAPAIRDTKGNFITEFLVTALLVFGLMGLGQVKMVDGFGPLTAGALIFVLGLSLGGPT
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCC
GYAINPARDLAPRIAHAILPIAGKGDSDWKYAWIPVLGPILGGILGALLFMLVF
CCEECCHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SIYMAEFLGTMMLIWLGDGVVASVALNKSKGKDGGWIVVTAAWGLAVAIPAYIFGGISG
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCC
AHMNPAVTIGNAIAGNFPWANVAGYITAQMLGGFAGAVLVWLTYLPHWKATEDKVTKLGV
CCCCCCEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHE
FCTAPAIRDTKGNFITEFLVTALLVFGLMGLGQVKMVDGFGPLTAGALIFVLGLSLGGPT
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCC
GYAINPARDLAPRIAHAILPIAGKGDSDWKYAWIPVLGPILGGILGALLFMLVF
CCEECCHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]