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The map label for this gene is cisA [H]

Identifier: 226949641

GI number: 226949641

Start: 2611695

End: 2613317

Strand: Direct

Name: cisA [H]

Synonym: CLM_2578

Alternate gene names: 226949641

Gene position: 2611695-2613317 (Clockwise)

Preceding gene: 226949640

Following gene: 226949642

Centisome position: 62.85

GC content: 28.65

Gene sequence:

>1623_bases
ATGAAAGCAATAGCAATATATGCTCGTAAATCTCTTTTCACAGGTAAAGGCGATTCTATAGGAGCCCAAGTAGATACTTG
TAAGCGATTTATAGATTATAAATTTGCTAATGAAGATTATGAAATTAGAACATTTAAAGATGAGGGTTGGAGTGGTAAAA
CTACAGATAGACCTGACTTTACTAATATGGTAAATCTAATTAAAAGTAAAAAAATAGATTATGTAATAACTTATAAACTA
GATCGTATAGGGAGAACTGCAAGAGATCTACATAACTTTCTATATGAATTAGATAATTTGGGTATTGTATATCTTAGTGC
TACTGAACCATACGATACTACAACATCTGCTGGTAGATTTATGATTTCTATATTGGCAGCTATGGCACAAATGGAAAGAG
AAAGACTTGCCGAAAGAGTTAAATCTGGGATGATACAAATAGCTAAAAAAGGCAGATGGCTTGGTGGTCAATGTCCCTTA
GGATTTGATTCTAAAAGAGAAATTTATATTGATGATATGGGTAAAGAACGTCAAATGATGAGACTAACCCCAAATAAAGA
GGAAATAAAAATTGTTAAGCTTATATATGATAAATATTTAGAAATGGGTAGTATGAGCCAGGTAAGAAAATATTGTTTAG
AAAACAGTATAAGAGGTAAAAATGGTGGTGATTTCTCTACAAATACACTTAAACAATTGCTTACTTCTCCCATCTATGTA
AAAAGTTCCGATAATATATTTAAGTATTTAGAATCACAAAATATAAATGTCTTTGGGACTCCAAATGGTAATGGTATGTT
AACTTTTAATAAAACTAAAGAAATAAGAATAGAAAGAGATAAATCAGAATGGATTGCTGCAGTTGGAAAACATAAAGGCA
TTATTGATGATAATAAATGGCTACAAATCCAACAACAATTACAACAACAATCTGAAAAACAAATTAAGAGTTCTGGAAGA
CAAGGTACTACATCTACAGGTTTACTTTCTGGAATTATAAAATGTTCAAAATGCGGTAATAACTTGCTTATAAAAACTGG
CCATAAAAGCAAAAAAAATCCTGGAACTACCTATAGTTATTATGTATGTGGTAAAAAAGATAATTCTTATGGCCATAAAT
GTGACAATAAAAATGTCAGAACAGATGAAGCAGACTCTGCCGTTATAACTCAACTTAAATTATATAATAAGGAGCTTCTA
ATAAAAAATCTTAAAGAAGCTTTAATTCAGAATGAAAAAACTGATACAGATAATATTGAAATATTAGAGAGTAAGCTAAA
AGAAAAAGAGAAAGCTGTCTCCAACCTTGTGAAAAAGCTTTCCCTTATTGATGATGAAAGTATCTCTAATATAATTTTAA
ATGAAGTAACTAACATTAACAAGGAAATTAATGATATTAAGTTACAACTTTCTAATGAAACTTTAAAAATTAATGAAGTA
ACTAAAGCTACCTTAGACACAGAAATTTATATAAAAATATTAGAAAACTTCAATAAAAAAATAGATGATATTACAGATCC
TATAGAAAAAATGAATCTTTTAAAAAGTGCTTTAGAAAGTGTTGAGTGGAATGGCGATTCTGGAGAGTTTAAAATTAACT
TAATAGGTTCTAAAAAAAAATAG

Upstream 100 bases:

>100_bases
GCATAAAGTCTCATATTTTATGGGCTTTTATTTTATACCTTATTTTGCATATTTACCTTTTAAGTTAATAAATATAAAAT
TGTATATGAGGTGATTACTT

Downstream 100 bases:

>100_bases
AACCCCTCTTTCCAAAAGAATCCAAATTATCGTACTTTAACATAGGAAACATGCCCTTGTCATTTAGCTGAAACAGGAGA
TTGTATATTATGTTCTCAGC

Product: resolvase

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKAIAIYARKSLFTGKGDSIGAQVDTCKRFIDYKFANEDYEIRTFKDEGWSGKTTDRPDFTNMVNLIKSKKIDYVITYKL
DRIGRTARDLHNFLYELDNLGIVYLSATEPYDTTTSAGRFMISILAAMAQMERERLAERVKSGMIQIAKKGRWLGGQCPL
GFDSKREIYIDDMGKERQMMRLTPNKEEIKIVKLIYDKYLEMGSMSQVRKYCLENSIRGKNGGDFSTNTLKQLLTSPIYV
KSSDNIFKYLESQNINVFGTPNGNGMLTFNKTKEIRIERDKSEWIAAVGKHKGIIDDNKWLQIQQQLQQQSEKQIKSSGR
QGTTSTGLLSGIIKCSKCGNNLLIKTGHKSKKNPGTTYSYYVCGKKDNSYGHKCDNKNVRTDEADSAVITQLKLYNKELL
IKNLKEALIQNEKTDTDNIEILESKLKEKEKAVSNLVKKLSLIDDESISNIILNEVTNINKEINDIKLQLSNETLKINEV
TKATLDTEIYIKILENFNKKIDDITDPIEKMNLLKSALESVEWNGDSGEFKINLIGSKKK

Sequences:

>Translated_540_residues
MKAIAIYARKSLFTGKGDSIGAQVDTCKRFIDYKFANEDYEIRTFKDEGWSGKTTDRPDFTNMVNLIKSKKIDYVITYKL
DRIGRTARDLHNFLYELDNLGIVYLSATEPYDTTTSAGRFMISILAAMAQMERERLAERVKSGMIQIAKKGRWLGGQCPL
GFDSKREIYIDDMGKERQMMRLTPNKEEIKIVKLIYDKYLEMGSMSQVRKYCLENSIRGKNGGDFSTNTLKQLLTSPIYV
KSSDNIFKYLESQNINVFGTPNGNGMLTFNKTKEIRIERDKSEWIAAVGKHKGIIDDNKWLQIQQQLQQQSEKQIKSSGR
QGTTSTGLLSGIIKCSKCGNNLLIKTGHKSKKNPGTTYSYYVCGKKDNSYGHKCDNKNVRTDEADSAVITQLKLYNKELL
IKNLKEALIQNEKTDTDNIEILESKLKEKEKAVSNLVKKLSLIDDESISNIILNEVTNINKEINDIKLQLSNETLKINEV
TKATLDTEIYIKILENFNKKIDDITDPIEKMNLLKSALESVEWNGDSGEFKINLIGSKKK
>Mature_540_residues
MKAIAIYARKSLFTGKGDSIGAQVDTCKRFIDYKFANEDYEIRTFKDEGWSGKTTDRPDFTNMVNLIKSKKIDYVITYKL
DRIGRTARDLHNFLYELDNLGIVYLSATEPYDTTTSAGRFMISILAAMAQMERERLAERVKSGMIQIAKKGRWLGGQCPL
GFDSKREIYIDDMGKERQMMRLTPNKEEIKIVKLIYDKYLEMGSMSQVRKYCLENSIRGKNGGDFSTNTLKQLLTSPIYV
KSSDNIFKYLESQNINVFGTPNGNGMLTFNKTKEIRIERDKSEWIAAVGKHKGIIDDNKWLQIQQQLQQQSEKQIKSSGR
QGTTSTGLLSGIIKCSKCGNNLLIKTGHKSKKNPGTTYSYYVCGKKDNSYGHKCDNKNVRTDEADSAVITQLKLYNKELL
IKNLKEALIQNEKTDTDNIEILESKLKEKEKAVSNLVKKLSLIDDESISNIILNEVTNINKEINDIKLQLSNETLKINEV
TKATLDTEIYIKILENFNKKIDDITDPIEKMNLLKSALESVEWNGDSGEFKINLIGSKKK

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=109, Percent_Identity=36.697247706422, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787827, Length=120, Percent_Identity=30.8333333333333, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1787638, Length=120, Percent_Identity=30.8333333333333, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 61505; Mature: 61505

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIAIYARKSLFTGKGDSIGAQVDTCKRFIDYKFANEDYEIRTFKDEGWSGKTTDRPDF
CCEEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCCCH
TNMVNLIKSKKIDYVITYKLDRIGRTARDLHNFLYELDNLGIVYLSATEPYDTTTSAGRF
HHHHHHHHHCCCCEEEEEEHHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
MISILAAMAQMERERLAERVKSGMIQIAKKGRWLGGQCPLGFDSKREIYIDDMGKERQMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHE
RLTPNKEEIKIVKLIYDKYLEMGSMSQVRKYCLENSIRGKNGGDFSTNTLKQLLTSPIYV
ECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCEEE
KSSDNIFKYLESQNINVFGTPNGNGMLTFNKTKEIRIERDKSEWIAAVGKHKGIIDDNKW
ECCCHHHHHHHCCCCEEEECCCCCEEEEECCCCEEEEECCHHHHHHHHHCCCCCCCCCHH
LQIQQQLQQQSEKQIKSSGRQGTTSTGLLSGIIKCSKCGNNLLIKTGHKSKKNPGTTYSY
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEE
YVCGKKDNSYGHKCDNKNVRTDEADSAVITQLKLYNKELLIKNLKEALIQNEKTDTDNIE
EEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ILESKLKEKEKAVSNLVKKLSLIDDESISNIILNEVTNINKEINDIKLQLSNETLKINEV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEHH
TKATLDTEIYIKILENFNKKIDDITDPIEKMNLLKSALESVEWNGDSGEFKINLIGSKKK
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MKAIAIYARKSLFTGKGDSIGAQVDTCKRFIDYKFANEDYEIRTFKDEGWSGKTTDRPDF
CCEEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCCCH
TNMVNLIKSKKIDYVITYKLDRIGRTARDLHNFLYELDNLGIVYLSATEPYDTTTSAGRF
HHHHHHHHHCCCCEEEEEEHHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
MISILAAMAQMERERLAERVKSGMIQIAKKGRWLGGQCPLGFDSKREIYIDDMGKERQMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHE
RLTPNKEEIKIVKLIYDKYLEMGSMSQVRKYCLENSIRGKNGGDFSTNTLKQLLTSPIYV
ECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCEEE
KSSDNIFKYLESQNINVFGTPNGNGMLTFNKTKEIRIERDKSEWIAAVGKHKGIIDDNKW
ECCCHHHHHHHCCCCEEEECCCCCEEEEECCCCEEEEECCHHHHHHHHHCCCCCCCCCHH
LQIQQQLQQQSEKQIKSSGRQGTTSTGLLSGIIKCSKCGNNLLIKTGHKSKKNPGTTYSY
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEE
YVCGKKDNSYGHKCDNKNVRTDEADSAVITQLKLYNKELLIKNLKEALIQNEKTDTDNIE
EEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ILESKLKEKEKAVSNLVKKLSLIDDESISNIILNEVTNINKEINDIKLQLSNETLKINEV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEHH
TKATLDTEIYIKILENFNKKIDDITDPIEKMNLLKSALESVEWNGDSGEFKINLIGSKKK
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]