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The map label for this gene is vanRB [H]

Identifier: 226949267

GI number: 226949267

Start: 2248201

End: 2248866

Strand: Reverse

Name: vanRB [H]

Synonym: CLM_2186

Alternate gene names: 226949267

Gene position: 2248866-2248201 (Counterclockwise)

Preceding gene: 226949268

Following gene: 226949266

Centisome position: 54.12

GC content: 29.13

Gene sequence:

>666_bases
ATGCCAAAGAGAATTCTTGTAGTAGAGGACGATTTTGATATACATACTATTATAAGTGAGGTTCTAAAGGAAAGTGGATA
TTTAGTGGAGGTGGCTACAGATGGATTAATTGCGGTGGAGATGTTTAGAAGGGGGAATTTTGATTTAATAATTTTAGATG
TGATGCTTCCTAAAATAGATGGATTTGTAGTATGTGAAATTATAAGAAAAGAATCAAGTGTGCCTATAATAATGCTTACT
GCTCTAGGGGAAGAGGAGGATGAGATGAAAGGTTTTGAATTAAAAGTAGACGATTATATAACAAAACCCTTTTCTATTAA
TTTATTAATAAAAAGAGTAGAAGCAGTACTTAGAAGAGCCAATGGATTAGAGGAAAACAATTCAACTTTAACCTTTGAAG
AAATAACAATATATCCTTCTAATTATAAAACTTTAGTTAATAATAAGGAAATAGAACTAACTTATAAAGAATTTCAGATA
TTAGAAATATTAATGTCAAATGTAGGAAGGGTCTTTTCTAGAGAATCTTTATTAAATCAAATATGGGGATATGATTATTT
TGGAGATACCCGTGTTATAGATACTCATATCAAAAATTTAAGACAAAAACTAAACATAGATTATATAAAGACCATAAGGG
GAGTGGGGTATAAAATTGAGAAATAA

Upstream 100 bases:

>100_bases
TCTGTTTATGAATGACATATAAGGTGAATATTGTAAATATTTTAATATTTAAAAATGATCATGTATGATAATGATATATT
TAAACAGGAGATGGATTACA

Downstream 100 bases:

>100_bases
ATTAAAAAAAAGTATAGTATATAAGCTATTTTTAGTAACTACATTATTATTAGTAATAAGTTCTATATGCACTTACTCTA
TAATTTATATGCTGCTACCT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MPKRILVVEDDFDIHTIISEVLKESGYLVEVATDGLIAVEMFRRGNFDLIILDVMLPKIDGFVVCEIIRKESSVPIIMLT
ALGEEEDEMKGFELKVDDYITKPFSINLLIKRVEAVLRRANGLEENNSTLTFEEITIYPSNYKTLVNNKEIELTYKEFQI
LEILMSNVGRVFSRESLLNQIWGYDYFGDTRVIDTHIKNLRQKLNIDYIKTIRGVGYKIEK

Sequences:

>Translated_221_residues
MPKRILVVEDDFDIHTIISEVLKESGYLVEVATDGLIAVEMFRRGNFDLIILDVMLPKIDGFVVCEIIRKESSVPIIMLT
ALGEEEDEMKGFELKVDDYITKPFSINLLIKRVEAVLRRANGLEENNSTLTFEEITIYPSNYKTLVNNKEIELTYKEFQI
LEILMSNVGRVFSRESLLNQIWGYDYFGDTRVIDTHIKNLRQKLNIDYIKTIRGVGYKIEK
>Mature_220_residues
PKRILVVEDDFDIHTIISEVLKESGYLVEVATDGLIAVEMFRRGNFDLIILDVMLPKIDGFVVCEIIRKESSVPIIMLTA
LGEEEDEMKGFELKVDDYITKPFSINLLIKRVEAVLRRANGLEENNSTLTFEEITIYPSNYKTLVNNKEIELTYKEFQIL
EILMSNVGRVFSRESLLNQIWGYDYFGDTRVIDTHIKNLRQKLNIDYIKTIRGVGYKIEK

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=36.4444444444444, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=35.5855855855856, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI87082012, Length=219, Percent_Identity=34.2465753424658, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=36.9369369369369, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1789809, Length=225, Percent_Identity=35.1111111111111, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789402, Length=217, Percent_Identity=33.1797235023041, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI2367329, Length=225, Percent_Identity=37.3333333333333, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=33.0316742081448, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1790552, Length=217, Percent_Identity=32.258064516129, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI1786911, Length=222, Percent_Identity=31.981981981982, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=33.7662337662338, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787229, Length=224, Percent_Identity=30.8035714285714, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=31.0810810810811, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=28.82096069869, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=28.0701754385965, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790437, Length=131, Percent_Identity=28.2442748091603, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788724, Length=140, Percent_Identity=29.2857142857143, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=28.3333333333333, Blast_Score=60, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=103, Percent_Identity=30.0970873786408, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25621; Mature: 25490

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRILVVEDDFDIHTIISEVLKESGYLVEVATDGLIAVEMFRRGNFDLIILDVMLPKID
CCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCEEEEEHHHCCCCCEEEEEEECCCCC
GFVVCEIIRKESSVPIIMLTALGEEEDEMKGFELKVDDYITKPFSINLLIKRVEAVLRRA
CCEEEEEHHCCCCCCEEEEEECCCCHHHCCCEEEEEHHHCCCCEEHHHHHHHHHHHHHHH
NGLEENNSTLTFEEITIYPSNYKTLVNNKEIELTYKEFQILEILMSNVGRVFSRESLLNQ
CCCCCCCCEEEEEEEEEECCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
IWGYDYFGDTRVIDTHIKNLRQKLNIDYIKTIRGVGYKIEK
HCCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHHCCCEEECC
>Mature Secondary Structure 
PKRILVVEDDFDIHTIISEVLKESGYLVEVATDGLIAVEMFRRGNFDLIILDVMLPKID
CCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCEEEEEHHHCCCCCEEEEEEECCCCC
GFVVCEIIRKESSVPIIMLTALGEEEDEMKGFELKVDDYITKPFSINLLIKRVEAVLRRA
CCEEEEEHHCCCCCCEEEEEECCCCHHHCCCEEEEEHHHCCCCEEHHHHHHHHHHHHHHH
NGLEENNSTLTFEEITIYPSNYKTLVNNKEIELTYKEFQILEILMSNVGRVFSRESLLNQ
CCCCCCCCEEEEEEEEEECCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
IWGYDYFGDTRVIDTHIKNLRQKLNIDYIKTIRGVGYKIEK
HCCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8631706; 12663927 [H]