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The map label for this gene is dapF [H]

Identifier: 226949210

GI number: 226949210

Start: 2178372

End: 2179193

Strand: Reverse

Name: dapF [H]

Synonym: CLM_2129

Alternate gene names: 226949210

Gene position: 2179193-2178372 (Counterclockwise)

Preceding gene: 226949213

Following gene: 226949209

Centisome position: 52.44

GC content: 32.12

Gene sequence:

>822_bases
ATGAAGTTTACTAAAATGACTGGAACTGGTAATGATTTTATAGTTTTAGATGACAGAAAGGGAAGTTTGATAGGGAAAGA
GAAAGAATTAGCACAAAAGTTATGTAATAGACATTTTAGTATAGGGGCAGATGGCATTTTATTTGTAAGGGAAAGTGATA
CCGATGCTCAGATACAAATGGTAATAATAAATGCAGATGGTTCTTATGCTTCTATGTGTGGAAATGGTATAAGATGTTTT
GCAAAGTATGTATGGGAAAAATCTATAGTAAAAGAAAATCCTATGATAATAGAAACAGGAGATGGCTTAAAGGAGGCTTT
TTTAGATATTGAAGAGGATAAAGTAAAATATATTACTATTAACATGGGTGAACCAATATTTGAAGGAGAAAAAATATCCA
ATGAAAAGGAAAAAATAATGAATAAAAGTATTAGGGAAAATAACAAAGAATATTCTATAACAGCCTTTCATATGGGAGTT
CCCCATACTATAATATTTGGCAATTTAGATGAATATGATATAGGAGAAGGTAAAAATATAGAAAGGCTACCTTTATTTAA
GGAGGGAACTAATGTAAACTTCTGCGAGGTAGTGGATAAAAATAAAATAAAGGTAAAAACCTGGGAAAGGGGTGCAGGAC
CAACTTTAGCTTGTGGTACTGGAAGTTGTGCATCAGCTATAGCATCTAATTTATTGGGCTATATAGGAAAGTCCACAGAG
GTAATATTGCCAGGGGGCAAACTTTTCATAGAAATTAAAGAAAATAGTGTTTTTATGAAGGGCCCTGCGGATATTTGTTT
TAGAGGAGAAATAGATGTATAA

Upstream 100 bases:

>100_bases
ATTTTTAGGAAATTAAACTAAAATAAAGTTATATTTTATGTAATATTAAAAGCTAATAATAAAAATAATTATAAGTAGTA
ATGTAAAGGATGTGACAATT

Downstream 100 bases:

>100_bases
ATGTTTAAGAAAGGCTTTAAAAATATAGGATATTTTATAATAGTTTTTTTAGTTATTGGCTTAGTTGGGTGTAAAAGCCA
TAGAATATCAGAAGGAGAAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKFTKMTGTGNDFIVLDDRKGSLIGKEKELAQKLCNRHFSIGADGILFVRESDTDAQIQMVIINADGSYASMCGNGIRCF
AKYVWEKSIVKENPMIIETGDGLKEAFLDIEEDKVKYITINMGEPIFEGEKISNEKEKIMNKSIRENNKEYSITAFHMGV
PHTIIFGNLDEYDIGEGKNIERLPLFKEGTNVNFCEVVDKNKIKVKTWERGAGPTLACGTGSCASAIASNLLGYIGKSTE
VILPGGKLFIEIKENSVFMKGPADICFRGEIDV

Sequences:

>Translated_273_residues
MKFTKMTGTGNDFIVLDDRKGSLIGKEKELAQKLCNRHFSIGADGILFVRESDTDAQIQMVIINADGSYASMCGNGIRCF
AKYVWEKSIVKENPMIIETGDGLKEAFLDIEEDKVKYITINMGEPIFEGEKISNEKEKIMNKSIRENNKEYSITAFHMGV
PHTIIFGNLDEYDIGEGKNIERLPLFKEGTNVNFCEVVDKNKIKVKTWERGAGPTLACGTGSCASAIASNLLGYIGKSTE
VILPGGKLFIEIKENSVFMKGPADICFRGEIDV
>Mature_273_residues
MKFTKMTGTGNDFIVLDDRKGSLIGKEKELAQKLCNRHFSIGADGILFVRESDTDAQIQMVIINADGSYASMCGNGIRCF
AKYVWEKSIVKENPMIIETGDGLKEAFLDIEEDKVKYITINMGEPIFEGEKISNEKEKIMNKSIRENNKEYSITAFHMGV
PHTIIFGNLDEYDIGEGKNIERLPLFKEGTNVNFCEVVDKNKIKVKTWERGAGPTLACGTGSCASAIASNLLGYIGKSTE
VILPGGKLFIEIKENSVFMKGPADICFRGEIDV

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=283, Percent_Identity=33.5689045936396, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30261; Mature: 30261

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTKMTGTGNDFIVLDDRKGSLIGKEKELAQKLCNRHFSIGADGILFVRESDTDAQIQM
CCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEE
VIINADGSYASMCGNGIRCFAKYVWEKSIVKENPMIIETGDGLKEAFLDIEEDKVKYITI
EEEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCEEEEEE
NMGEPIFEGEKISNEKEKIMNKSIRENNKEYSITAFHMGVPHTIIFGNLDEYDIGEGKNI
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCC
ERLPLFKEGTNVNFCEVVDKNKIKVKTWERGAGPTLACGTGSCASAIASNLLGYIGKSTE
CCCCCEECCCCCEEEEEECCCCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCCE
VILPGGKLFIEIKENSVFMKGPADICFRGEIDV
EEECCCEEEEEECCCEEEEECCCCEEEECCCCC
>Mature Secondary Structure
MKFTKMTGTGNDFIVLDDRKGSLIGKEKELAQKLCNRHFSIGADGILFVRESDTDAQIQM
CCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEE
VIINADGSYASMCGNGIRCFAKYVWEKSIVKENPMIIETGDGLKEAFLDIEEDKVKYITI
EEEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCEEEEEE
NMGEPIFEGEKISNEKEKIMNKSIRENNKEYSITAFHMGVPHTIIFGNLDEYDIGEGKNI
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCC
ERLPLFKEGTNVNFCEVVDKNKIKVKTWERGAGPTLACGTGSCASAIASNLLGYIGKSTE
CCCCCEECCCCCEEEEEECCCCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCCE
VILPGGKLFIEIKENSVFMKGPADICFRGEIDV
EEECCCEEEEEECCCEEEEECCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842; 7559358 [H]