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The map label for this gene is punA [H]

Identifier: 226949107

GI number: 226949107

Start: 2092426

End: 2093241

Strand: Reverse

Name: punA [H]

Synonym: CLM_2025

Alternate gene names: 226949107

Gene position: 2093241-2092426 (Counterclockwise)

Preceding gene: 226949108

Following gene: 226949106

Centisome position: 50.38

GC content: 31.37

Gene sequence:

>816_bases
TTGAATTTAAAGAAAATAAAAGAAAGTGCAGATTTTGTGAAAAATAACATATCTATAATGCCTGAAATTGGAGTTGTTTT
AGGCTCAGGCTTAGGTGATTTAGCTGAGGAAATAGAAAATAAAGAAATTATAAAATATTCCAATATACCTAATATGCCAG
CTTCAACTATAAAAGGACATAAGGGTCAGTTTGTAGTGGGAACTTTAAAGGGTAAAAAGGTTATAATGATGCAAGGGAGA
GTGCATTATTATGAAGGAAATAAAATGAGCGATGTAGTACTTCCTGTATACATAATGAAAGAGCTAGGAGTAAAAAATAT
AATTGTTACAAATGCAGCTGGGGGAGTAAATGAAATTTATAATCCTGGAGATCTTATGATTATAGAAGATCATATTAATT
TTGCTTTTAGTAATCCTTTAATAGGTAGTAATGATGAGGAAATTGGACCAAGATTTCCAGATATGTCAGAAGCATATAAT
AAAAGTTTGATAGTCCTTGCAGAAAAAGAAGCTGAAAAATTAGGCTTTTCTCTTCAAAAGGGAGTATATGCTATGATGAC
AGGACCTTCATATGAAACACCAGCAGAAATAAAAATGCTAAGAATGTTAGGGGCAGATGCAGTAGGAATGTCTACAGTAC
CAGAGGTTATAGCAGCAAATCATTGTGGAATTAAAGTATTGGGAATATCTTGTATAACTAATATGGCAGCAGGTATATTA
GAGCAGCCACTAAACCATCAAGAAGTTATAGAAACATCTAATAAAGTAAGATCTAAATTCATATCCTTACTAAAATCAAT
ATTAGAAGTTATGTAA

Upstream 100 bases:

>100_bases
TCTAGTATATCAAAGAAGAGTAAAATAGCAGAAGTATATAAAAATGCTCATCCAAGGGCATAATTTTAATAAAGCTTTGA
AGATAAAGGAGGAGTTAATT

Downstream 100 bases:

>100_bases
AATTTTTAATAATAAAAAATGGGTTGATAAGTGATTCTTAGGGTCAAATATAGAAATGTTTTGTATAAACAAGCTCTACT
TGATCCTAACAATCACTTAA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNLKKIKESADFVKNNISIMPEIGVVLGSGLGDLAEEIENKEIIKYSNIPNMPASTIKGHKGQFVVGTLKGKKVIMMQGR
VHYYEGNKMSDVVLPVYIMKELGVKNIIVTNAAGGVNEIYNPGDLMIIEDHINFAFSNPLIGSNDEEIGPRFPDMSEAYN
KSLIVLAEKEAEKLGFSLQKGVYAMMTGPSYETPAEIKMLRMLGADAVGMSTVPEVIAANHCGIKVLGISCITNMAAGIL
EQPLNHQEVIETSNKVRSKFISLLKSILEVM

Sequences:

>Translated_271_residues
MNLKKIKESADFVKNNISIMPEIGVVLGSGLGDLAEEIENKEIIKYSNIPNMPASTIKGHKGQFVVGTLKGKKVIMMQGR
VHYYEGNKMSDVVLPVYIMKELGVKNIIVTNAAGGVNEIYNPGDLMIIEDHINFAFSNPLIGSNDEEIGPRFPDMSEAYN
KSLIVLAEKEAEKLGFSLQKGVYAMMTGPSYETPAEIKMLRMLGADAVGMSTVPEVIAANHCGIKVLGISCITNMAAGIL
EQPLNHQEVIETSNKVRSKFISLLKSILEVM
>Mature_271_residues
MNLKKIKESADFVKNNISIMPEIGVVLGSGLGDLAEEIENKEIIKYSNIPNMPASTIKGHKGQFVVGTLKGKKVIMMQGR
VHYYEGNKMSDVVLPVYIMKELGVKNIIVTNAAGGVNEIYNPGDLMIIEDHINFAFSNPLIGSNDEEIGPRFPDMSEAYN
KSLIVLAEKEAEKLGFSLQKGVYAMMTGPSYETPAEIKMLRMLGADAVGMSTVPEVIAANHCGIKVLGISCITNMAAGIL
EQPLNHQEVIETSNKVRSKFISLLKSILEVM

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=269, Percent_Identity=45.3531598513011, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI47132622, Length=259, Percent_Identity=29.3436293436293, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788746, Length=249, Percent_Identity=40.9638554216867, Blast_Score=214, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17541190, Length=250, Percent_Identity=43.2, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6323238, Length=271, Percent_Identity=39.8523985239852, Blast_Score=198, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24656090, Length=273, Percent_Identity=44.6886446886447, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI45552887, Length=273, Percent_Identity=44.6886446886447, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI45552885, Length=273, Percent_Identity=44.6886446886447, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24656093, Length=273, Percent_Identity=44.6886446886447, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24762376, Length=240, Percent_Identity=35.8333333333333, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI221459247, Length=159, Percent_Identity=30.8176100628931, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20130079, Length=249, Percent_Identity=24.0963855421687, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29575; Mature: 29575

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKKIKESADFVKNNISIMPEIGVVLGSGLGDLAEEIENKEIIKYSNIPNMPASTIKGH
CCHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHCCCCEEEECCCCCCCHHHCCCC
KGQFVVGTLKGKKVIMMQGRVHYYEGNKMSDVVLPVYIMKELGVKNIIVTNAAGGVNEIY
CCCEEEEEECCCEEEEEECEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCC
NPGDLMIIEDHINFAFSNPLIGSNDEEIGPRFPDMSEAYNKSLIVLAEKEAEKLGFSLQK
CCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCHHHHCCHHHC
GVYAMMTGPSYETPAEIKMLRMLGADAVGMSTVPEVIAANHCGIKVLGISCITNMAAGIL
CEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
EQPLNHQEVIETSNKVRSKFISLLKSILEVM
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNLKKIKESADFVKNNISIMPEIGVVLGSGLGDLAEEIENKEIIKYSNIPNMPASTIKGH
CCHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHCCCCEEEECCCCCCCHHHCCCC
KGQFVVGTLKGKKVIMMQGRVHYYEGNKMSDVVLPVYIMKELGVKNIIVTNAAGGVNEIY
CCCEEEEEECCCEEEEEECEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCC
NPGDLMIIEDHINFAFSNPLIGSNDEEIGPRFPDMSEAYNKSLIVLAEKEAEKLGFSLQK
CCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCHHHHCCHHHC
GVYAMMTGPSYETPAEIKMLRMLGADAVGMSTVPEVIAANHCGIKVLGISCITNMAAGIL
CEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
EQPLNHQEVIETSNKVRSKFISLLKSILEVM
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]