| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is metH [H]
Identifier: 226948951
GI number: 226948951
Start: 1902980
End: 1905358
Strand: Direct
Name: metH [H]
Synonym: CLM_1865
Alternate gene names: 226948951
Gene position: 1902980-1905358 (Clockwise)
Preceding gene: 226948950
Following gene: 226948953
Centisome position: 45.8
GC content: 29.84
Gene sequence:
>2379_bases GTGAATATTAAGGATTATATAAAAGAAAATGTATTAATATTTGATGGTGCCATGGGAACAATGCTTCAAAAATTAGGGTT AAAGATTTCAGATTTGCCAGAAGAATTAAATATATTAGAACCTGAAAAAATAATAAACATACATAGAAAATATATAGAAA CAGGAGCAAAGGTTATAACAACCAATACCTTTGGAGCAAATGAAATAAAGCTTAAACAAAGTAAGTTTTCTTTAGAAAGC ATTATAGATAAAGCAATAGATAACGTGAAAAAAGCAGGGAAAAACAAAGAAATACTTATAGCATTAGATATAGGTCCAAT AGGACAGCTTTTAGAACCTATGGGAACATTAAAATTTGAAGAAGCCTATGAAATTTTTAAAAGACAAATTGTACAAGGAC AAAAAAGTGGGGCAGATATAGTTTTAATAGAAACAATGACGGATCTTTATGAAGCTAAAGCTGCTATTTTGGCAGCAAAA GAAAATACAAATCTCCCTGTATTTTGCACTATGACCTTTGAAAAAAATAAGAGAACTTTTACAGGATGCACTCCATTATC TATGGTTCTTACTTTAGAGGGACTAGGGGTAGATGCTTTAGGTGTCAATTGTTCCCTAGGGCCAAATGAATTAGGAGATA TTGTAGATGAAATAATAAAATATTCAAGTATACCTATAATGGTTCAGCCTAATGCAGGTCTTCCAACTATTAAAGCGGGT AGAACTATTTATAATATTGAGCCTAAAGAATTTGCAGATTTTCAAAGGAGTATTGTAGAAAAGGGAGTAAGGATAGTAGG AGGCTGTTGTGGAACTACAGATGAATTTATAAGAGAAATTGTATATAGTCTAAAGGATGTAAAGATAAAAAAGCTAAAAG AAAAAAATATTTGTGGAGTATGTTCTTCTACAAAATCAGTTTTGATAGAGGGAGTTAAGATAATAGGAGAAAGAATTAAT CCAACGGGTAAAAGATTATTTAAAGAAGCTCTTAGAAATAATGATACAGATTATATATTGAAAGAAGCAATAAGTCAGGT TGAATGTGGTACAGATATATTAGATGTAAATGTAGGACTTCCAGAAATAAATGAAGAAGAAACAATGAAGAAAGTTGTAA AAGAGATTCAATCTATAATTGATGCACCTCTTCAAATAGATTCTAATAATCCTAAGGTTATAGAAAAAGCATTAAGAGTA TATAATGGAAAAGCTATAGTTAATTCTGTAAATGGAGAAGAGGAAGTACTAGACAGTGTACTACCATTAATAAAAAAATA TGGAGCTGCTGTTGTTGGATTAACTTTAGATGATAAGGGTATACCTAAAAAAGCTGAAGAAAGGCTAAAAGTAGCTGAGA AAATAGTTAATAAGGCATTAGAATATGGCATAAGAAGAGAAGATATATTTATTGATTGTTTGGTTCTAACTGCATCAGCA CAGCAAGGGGATGTTGGAGAAACCCTTAAAGCTGTAAATTTAGTTAAAGAGAAATTGAATGTGAAAACAATACTAGGAGT ATCTAATATATCCTTTGGATTACCTAATAGAGAACTTATAAATAAAACTTTTTTAGCTATGAGTCTTCAATCAGGATTGG ATTTACCTATATTAAATCCTAATAATAAAGAAATGATAAATATTATAAATGCCTATAAGGTTTTAAATAATGAGGACAAG AGAGCTGCAAATTATATAGAAAGGTATACCAATGAAATATCAAATAGTAGAGAAGTAAAAATCCCAAAAAACGATTTAAC TTTAAAGGAAATAGTTATGAAAGGAATAAAAGAGGAATCATATAGTAAGACAAAGGATCTTCTTAAAGATAGAGGTGAAC TTTCAATAATAAATGAAGAACTAATTCCTGCTCTAGATGAAGTTGGTGATAAATATGAAAAAGGTATAATATTTTTACCA CAGCTAATTCAATCTGCAGAAACGGTTAAAAAAGCTTTTACAGCTATAAAAGAAAAACTTAGAGAAGATAATTCACCAAA AATAAATAAAGGTAAAATATTAATGGCAACTGTTAAAGGTGATATTCATGATATAGGAAAAAATATAGTGAAGGTTATAC TAGAAAACTATGGATTTGATATTATAGATTTAGGTAAGGATGTTGAAGCGGAGAAGATAGTAGAAGAGGTAAAGAAAAAC AATATAAAGTTAGTAGGGTTAAGTGCTTTGATGACTACTACGGTAAATAGTATGAAAGATACCATAAAGGCTTTAAAGGA AAGCGAGATGGATTGTAAAGTTTTTGTTGGTGGAGCAGTTCTTAATAAGGAATATGCAGAAATGATAAATGCGGATTATT ATGCTAAGGATGCAAAGGAAGCAGTAGACATAGCCAAAGGATTCTTTGGAGGATTTTGA
Upstream 100 bases:
>100_bases ATTATAGGTGTAATTCCAAAGGAAGTTGTAGTAAATAAAAAATCTTGTAGTAAGTGCAATAAATTTAGTAGTTGTAAATT TAGAAAGATAGGTGAGATAT
Downstream 100 bases:
>100_bases AAATTAATCCAATAAAAATATTTAAGTTTATACAAAACACTTACCAGTATTGAAGGTAAGTGTTTTGTAGTTTTGTGTTA ATTAATTTTTACAAATAATA
Product: putative 5-methyltetrahydrofolate--homocysteine methyltransferase
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 792; Mature: 792
Protein sequence:
>792_residues MNIKDYIKENVLIFDGAMGTMLQKLGLKISDLPEELNILEPEKIINIHRKYIETGAKVITTNTFGANEIKLKQSKFSLES IIDKAIDNVKKAGKNKEILIALDIGPIGQLLEPMGTLKFEEAYEIFKRQIVQGQKSGADIVLIETMTDLYEAKAAILAAK ENTNLPVFCTMTFEKNKRTFTGCTPLSMVLTLEGLGVDALGVNCSLGPNELGDIVDEIIKYSSIPIMVQPNAGLPTIKAG RTIYNIEPKEFADFQRSIVEKGVRIVGGCCGTTDEFIREIVYSLKDVKIKKLKEKNICGVCSSTKSVLIEGVKIIGERIN PTGKRLFKEALRNNDTDYILKEAISQVECGTDILDVNVGLPEINEEETMKKVVKEIQSIIDAPLQIDSNNPKVIEKALRV YNGKAIVNSVNGEEEVLDSVLPLIKKYGAAVVGLTLDDKGIPKKAEERLKVAEKIVNKALEYGIRREDIFIDCLVLTASA QQGDVGETLKAVNLVKEKLNVKTILGVSNISFGLPNRELINKTFLAMSLQSGLDLPILNPNNKEMINIINAYKVLNNEDK RAANYIERYTNEISNSREVKIPKNDLTLKEIVMKGIKEESYSKTKDLLKDRGELSIINEELIPALDEVGDKYEKGIIFLP QLIQSAETVKKAFTAIKEKLREDNSPKINKGKILMATVKGDIHDIGKNIVKVILENYGFDIIDLGKDVEAEKIVEEVKKN NIKLVGLSALMTTTVNSMKDTIKALKESEMDCKVFVGGAVLNKEYAEMINADYYAKDAKEAVDIAKGFFGGF
Sequences:
>Translated_792_residues MNIKDYIKENVLIFDGAMGTMLQKLGLKISDLPEELNILEPEKIINIHRKYIETGAKVITTNTFGANEIKLKQSKFSLES IIDKAIDNVKKAGKNKEILIALDIGPIGQLLEPMGTLKFEEAYEIFKRQIVQGQKSGADIVLIETMTDLYEAKAAILAAK ENTNLPVFCTMTFEKNKRTFTGCTPLSMVLTLEGLGVDALGVNCSLGPNELGDIVDEIIKYSSIPIMVQPNAGLPTIKAG RTIYNIEPKEFADFQRSIVEKGVRIVGGCCGTTDEFIREIVYSLKDVKIKKLKEKNICGVCSSTKSVLIEGVKIIGERIN PTGKRLFKEALRNNDTDYILKEAISQVECGTDILDVNVGLPEINEEETMKKVVKEIQSIIDAPLQIDSNNPKVIEKALRV YNGKAIVNSVNGEEEVLDSVLPLIKKYGAAVVGLTLDDKGIPKKAEERLKVAEKIVNKALEYGIRREDIFIDCLVLTASA QQGDVGETLKAVNLVKEKLNVKTILGVSNISFGLPNRELINKTFLAMSLQSGLDLPILNPNNKEMINIINAYKVLNNEDK RAANYIERYTNEISNSREVKIPKNDLTLKEIVMKGIKEESYSKTKDLLKDRGELSIINEELIPALDEVGDKYEKGIIFLP QLIQSAETVKKAFTAIKEKLREDNSPKINKGKILMATVKGDIHDIGKNIVKVILENYGFDIIDLGKDVEAEKIVEEVKKN NIKLVGLSALMTTTVNSMKDTIKALKESEMDCKVFVGGAVLNKEYAEMINADYYAKDAKEAVDIAKGFFGGF >Mature_792_residues MNIKDYIKENVLIFDGAMGTMLQKLGLKISDLPEELNILEPEKIINIHRKYIETGAKVITTNTFGANEIKLKQSKFSLES IIDKAIDNVKKAGKNKEILIALDIGPIGQLLEPMGTLKFEEAYEIFKRQIVQGQKSGADIVLIETMTDLYEAKAAILAAK ENTNLPVFCTMTFEKNKRTFTGCTPLSMVLTLEGLGVDALGVNCSLGPNELGDIVDEIIKYSSIPIMVQPNAGLPTIKAG RTIYNIEPKEFADFQRSIVEKGVRIVGGCCGTTDEFIREIVYSLKDVKIKKLKEKNICGVCSSTKSVLIEGVKIIGERIN PTGKRLFKEALRNNDTDYILKEAISQVECGTDILDVNVGLPEINEEETMKKVVKEIQSIIDAPLQIDSNNPKVIEKALRV YNGKAIVNSVNGEEEVLDSVLPLIKKYGAAVVGLTLDDKGIPKKAEERLKVAEKIVNKALEYGIRREDIFIDCLVLTASA QQGDVGETLKAVNLVKEKLNVKTILGVSNISFGLPNRELINKTFLAMSLQSGLDLPILNPNNKEMINIINAYKVLNNEDK RAANYIERYTNEISNSREVKIPKNDLTLKEIVMKGIKEESYSKTKDLLKDRGELSIINEELIPALDEVGDKYEKGIIFLP QLIQSAETVKKAFTAIKEKLREDNSPKINKGKILMATVKGDIHDIGKNIVKVILENYGFDIIDLGKDVEAEKIVEEVKKN NIKLVGLSALMTTTVNSMKDTIKALKESEMDCKVFVGGAVLNKEYAEMINADYYAKDAKEAVDIAKGFFGGF
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=881, Percent_Identity=28.2633371169126, Blast_Score=282, Evalue=9e-76, Organism=Escherichia coli, GI1790450, Length=846, Percent_Identity=28.9598108747045, Blast_Score=298, Evalue=1e-81, Organism=Escherichia coli, GI1786456, Length=295, Percent_Identity=28.135593220339, Blast_Score=87, Evalue=4e-18, Organism=Caenorhabditis elegans, GI17535405, Length=883, Percent_Identity=28.7655719139298, Blast_Score=290, Evalue=3e-78, Organism=Saccharomyces cerevisiae, GI6322966, Length=322, Percent_Identity=24.8447204968944, Blast_Score=67, Evalue=1e-11,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 87752; Mature: 87752
Theoretical pI: Translated: 5.47; Mature: 5.47
Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNIKDYIKENVLIFDGAMGTMLQKLGLKISDLPEELNILEPEKIINIHRKYIETGAKVIT CCCHHHHHCCEEEEECHHHHHHHHHCCCHHCCCHHCCCCCHHHHHHHHHHHHHCCCEEEE TNTFGANEIKLKQSKFSLESIIDKAIDNVKKAGKNKEILIALDIGPIGQLLEPMGTLKFE ECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCHH EAYEIFKRQIVQGQKSGADIVLIETMTDLYEAKAAILAAKENTNLPVFCTMTFEKNKRTF HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHEEECCCCCCCEEEEEEEECCCCEE TGCTPLSMVLTLEGLGVDALGVNCSLGPNELGDIVDEIIKYSSIPIMVQPNAGLPTIKAG CCCCHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEECC RTIYNIEPKEFADFQRSIVEKGVRIVGGCCGTTDEFIREIVYSLKDVKIKKLKEKNICGV CEEEECCHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC CSSTKSVLIEGVKIIGERINPTGKRLFKEALRNNDTDYILKEAISQVECGTDILDVNVGL HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCC PEINEEETMKKVVKEIQSIIDAPLQIDSNNPKVIEKALRVYNGKAIVNSVNGEEEVLDSV CCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHH LPLIKKYGAAVVGLTLDDKGIPKKAEERLKVAEKIVNKALEYGIRREDIFIDCLVLTASA HHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEEECC QQGDVGETLKAVNLVKEKLNVKTILGVSNISFGLPNRELINKTFLAMSLQSGLDLPILNP CCCCHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECC NNKEMINIINAYKVLNNEDKRAANYIERYTNEISNSREVKIPKNDLTLKEIVMKGIKEES CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCHHH YSKTKDLLKDRGELSIINEELIPALDEVGDKYEKGIIFLPQLIQSAETVKKAFTAIKEKL HHHHHHHHHCCCCEEEECHHHHHHHHHHCHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHH REDNSPKINKGKILMATVKGDIHDIGKNIVKVILENYGFDIIDLGKDVEAEKIVEEVKKN HCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHC NIKLVGLSALMTTTVNSMKDTIKALKESEMDCKVFVGGAVLNKEYAEMINADYYAKDAKE CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHH AVDIAKGFFGGF HHHHHHHHCCCC >Mature Secondary Structure MNIKDYIKENVLIFDGAMGTMLQKLGLKISDLPEELNILEPEKIINIHRKYIETGAKVIT CCCHHHHHCCEEEEECHHHHHHHHHCCCHHCCCHHCCCCCHHHHHHHHHHHHHCCCEEEE TNTFGANEIKLKQSKFSLESIIDKAIDNVKKAGKNKEILIALDIGPIGQLLEPMGTLKFE ECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCHH EAYEIFKRQIVQGQKSGADIVLIETMTDLYEAKAAILAAKENTNLPVFCTMTFEKNKRTF HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHEEECCCCCCCEEEEEEEECCCCEE TGCTPLSMVLTLEGLGVDALGVNCSLGPNELGDIVDEIIKYSSIPIMVQPNAGLPTIKAG CCCCHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEECC RTIYNIEPKEFADFQRSIVEKGVRIVGGCCGTTDEFIREIVYSLKDVKIKKLKEKNICGV CEEEECCHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC CSSTKSVLIEGVKIIGERINPTGKRLFKEALRNNDTDYILKEAISQVECGTDILDVNVGL HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCC PEINEEETMKKVVKEIQSIIDAPLQIDSNNPKVIEKALRVYNGKAIVNSVNGEEEVLDSV CCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHH LPLIKKYGAAVVGLTLDDKGIPKKAEERLKVAEKIVNKALEYGIRREDIFIDCLVLTASA HHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEEECC QQGDVGETLKAVNLVKEKLNVKTILGVSNISFGLPNRELINKTFLAMSLQSGLDLPILNP CCCCHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECC NNKEMINIINAYKVLNNEDKRAANYIERYTNEISNSREVKIPKNDLTLKEIVMKGIKEES CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCHHH YSKTKDLLKDRGELSIINEELIPALDEVGDKYEKGIIFLPQLIQSAETVKKAFTAIKEKL HHHHHHHHHCCCCEEEECHHHHHHHHHHCHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHH REDNSPKINKGKILMATVKGDIHDIGKNIVKVILENYGFDIIDLGKDVEAEKIVEEVKKN HCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHC NIKLVGLSALMTTTVNSMKDTIKALKESEMDCKVFVGGAVLNKEYAEMINADYYAKDAKE CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHH AVDIAKGFFGGF HHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11234002 [H]