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The map label for this gene is metF [H]

Identifier: 226948949

GI number: 226948949

Start: 1901268

End: 1902155

Strand: Direct

Name: metF [H]

Synonym: CLM_1863

Alternate gene names: 226948949

Gene position: 1901268-1902155 (Clockwise)

Preceding gene: 226948947

Following gene: 226948950

Centisome position: 45.76

GC content: 27.25

Gene sequence:

>888_bases
ATGCATATAAAAGAATTATTTAATGAAAAAAAATTAGTTTTCTCTTTTGAAATTTTTCCACCAAAAGTTACATCATCTAT
AAAAACAATATATGAAACTTTAGAGGAGTTAAAAGATTTAACACCGGATTTCATAAGCGTAACTTACGGAGCTGGAGGAA
GCTTAAAGAATAATAAAACCTGTGAGTTATCATCTCTTGTAAAAAATAAATATGGTATAGAAGCATTGGCACATTTAACT
TGTATAAATTCGACAAAGGATGATATAGATTTAATAATAAAAGAATTGAAAGAAAATAATATTAAAAATATACTAGCTTT
AAGAGGAGATATACCAAAGGATAAAGATATTATTGGAGAGTATAATTATGCCTTTGAACTTATAGAAAAGATAAAGGCAA
GTAATAATTTTGGAATATCCGGAGCCTGTTATCCAGAAGGACATATTGAATGTAAAAGCTTAGATCAGGATATAAGAGAA
TTAAAAAGAAAGGTAGACGCAGGGGCAGAACATTTAATATCTCAATTATTTTTTGATAACAATATATTCTATGAATTTTT
AAATAAGACACAGGAAAAGAGCATAAATGTTCCTATACAAGCAGGAGTAATGCCTGTAGTTAACAAAAAACAAATAGAGA
GAATAGTTAAAATATCTGGTGCTACATTGCCTAAAAAGTTTATGAAAATATTAGATAAATATGAATATGATAAGAAAGCT
ATGGAAGATGCAGGCATAGCCTATGCAGTAGAACAGATAGTTGATTTAGTTTCTAGTGGAGTAAAGGGAATACATCTATA
TACAATGAACAATCCTTATATAGCTAGAAAAATAACAGAAAGTACAAAATCAATATTTAATTCTATAAATAAAGATATAG
CAGTTTAA

Upstream 100 bases:

>100_bases
AAGGGAAGTATCCTTTAGGTTTTTTATTTTTTAAGGTCTCTCAGAATTTAAATATTTATAGTAGATAAAAAATATAAAAA
AATTATTAGGAGGCTTTAAA

Downstream 100 bases:

>100_bases
AAACTAATACTTATATTAGTTCATGGTGTTTGCAGAGAAAATAGTGTTTATAAACAAATATGAAAAGATTTAGTATATAT
TTTTTTAATAAAAAGTGATA

Product: methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MHIKELFNEKKLVFSFEIFPPKVTSSIKTIYETLEELKDLTPDFISVTYGAGGSLKNNKTCELSSLVKNKYGIEALAHLT
CINSTKDDIDLIIKELKENNIKNILALRGDIPKDKDIIGEYNYAFELIEKIKASNNFGISGACYPEGHIECKSLDQDIRE
LKRKVDAGAEHLISQLFFDNNIFYEFLNKTQEKSINVPIQAGVMPVVNKKQIERIVKISGATLPKKFMKILDKYEYDKKA
MEDAGIAYAVEQIVDLVSSGVKGIHLYTMNNPYIARKITESTKSIFNSINKDIAV

Sequences:

>Translated_295_residues
MHIKELFNEKKLVFSFEIFPPKVTSSIKTIYETLEELKDLTPDFISVTYGAGGSLKNNKTCELSSLVKNKYGIEALAHLT
CINSTKDDIDLIIKELKENNIKNILALRGDIPKDKDIIGEYNYAFELIEKIKASNNFGISGACYPEGHIECKSLDQDIRE
LKRKVDAGAEHLISQLFFDNNIFYEFLNKTQEKSINVPIQAGVMPVVNKKQIERIVKISGATLPKKFMKILDKYEYDKKA
MEDAGIAYAVEQIVDLVSSGVKGIHLYTMNNPYIARKITESTKSIFNSINKDIAV
>Mature_295_residues
MHIKELFNEKKLVFSFEIFPPKVTSSIKTIYETLEELKDLTPDFISVTYGAGGSLKNNKTCELSSLVKNKYGIEALAHLT
CINSTKDDIDLIIKELKENNIKNILALRGDIPKDKDIIGEYNYAFELIEKIKASNNFGISGACYPEGHIECKSLDQDIRE
LKRKVDAGAEHLISQLFFDNNIFYEFLNKTQEKSINVPIQAGVMPVVNKKQIERIVKISGATLPKKFMKILDKYEYDKKA
MEDAGIAYAVEQIVDLVSSGVKGIHLYTMNNPYIARKITESTKSIFNSINKDIAV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=265, Percent_Identity=32.0754716981132, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1790377, Length=267, Percent_Identity=35.2059925093633, Blast_Score=177, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25147436, Length=288, Percent_Identity=31.9444444444444, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI25147441, Length=288, Percent_Identity=31.9444444444444, Blast_Score=157, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6325234, Length=290, Percent_Identity=33.7931034482759, Blast_Score=153, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321313, Length=270, Percent_Identity=31.4814814814815, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24662658, Length=250, Percent_Identity=24.4, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33329; Mature: 33329

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIKELFNEKKLVFSFEIFPPKVTSSIKTIYETLEELKDLTPDFISVTYGAGGSLKNNKT
CCHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
CELSSLVKNKYGIEALAHLTCINSTKDDIDLIIKELKENNIKNILALRGDIPKDKDIIGE
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHCC
YNYAFELIEKIKASNNFGISGACYPEGHIECKSLDQDIRELKRKVDAGAEHLISQLFFDN
HHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
NIFYEFLNKTQEKSINVPIQAGVMPVVNKKQIERIVKISGATLPKKFMKILDKYEYDKKA
CHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
MEDAGIAYAVEQIVDLVSSGVKGIHLYTMNNPYIARKITESTKSIFNSINKDIAV
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHIKELFNEKKLVFSFEIFPPKVTSSIKTIYETLEELKDLTPDFISVTYGAGGSLKNNKT
CCHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
CELSSLVKNKYGIEALAHLTCINSTKDDIDLIIKELKENNIKNILALRGDIPKDKDIIGE
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHCC
YNYAFELIEKIKASNNFGISGACYPEGHIECKSLDQDIRELKRKVDAGAEHLISQLFFDN
HHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
NIFYEFLNKTQEKSINVPIQAGVMPVVNKKQIERIVKISGATLPKKFMKILDKYEYDKKA
CHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
MEDAGIAYAVEQIVDLVSSGVKGIHLYTMNNPYIARKITESTKSIFNSINKDIAV
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]