| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is exp5 [H]
Identifier: 226948934
GI number: 226948934
Start: 1881991
End: 1883649
Strand: Direct
Name: exp5 [H]
Synonym: CLM_1847
Alternate gene names: 226948934
Gene position: 1881991-1883649 (Clockwise)
Preceding gene: 226948933
Following gene: 226948935
Centisome position: 45.29
GC content: 31.89
Gene sequence:
>1659_bases ATGAAAAGGAATAAGCTAGTATCTTTTGATTTTTGGCAAAAATTTGGAAAGACACTATTAGTTGTTGTGGCAGTTATGCC TGCAGCAGGACTTATGATTTGTATTGGTAAACTTATAGGAATGCAGGCTTCTATGGGATTAGTACAGTCTGTAGCAAGAG TAGTAGAAGATATAGGATGGGCAATAATAGGAAATCTTCACATTTTATTTGCAGTAGCAATCGGTGGATCTTGGGCAAAA GAGCGTGCTGGAGGAGCTTTTGCAGGATTACTTACTTTTATTCTTACAAATAGAATAACAGGAGCTATTTTCGGAGTTAA ACCAGATATGTTTTCTGATGAAGCAGCAAAAGTAACTTCAATGTTTGGAAGACAATTAGTAGTTAAAGATTATTTTACAA CTATACTTGGAGCACCCGCATTAAATATGGGTGTATTTATAGGTATAATTTCAGGATTTCTAGGAGCGTACTTATATAAT AAATATTATAATTTTAATAAATTACCAAAATCTTTGGCTTTTTTTAATGGAAAAAGATTTGTACCATTTGTAGTTATACT AGGATCTGTAGTAACAGCAATAATTTTATCTATGATTTGGCCTTCTATTCAAGGTGCGTTAAATGCTTTTGGAAAATGGA TAGCAACATCTAAGGATACTGCACCAATAGTAGCACCTTTCATTTTTGGATCATTGGAACGTATATTATTACCTTTTGGA CTTCATCATATGTTAACAGTACCGCTAAATTATACTTCTCTTGGAGGAACTTATAAAATTTTAACAGGGCCAACTGCAGG TACTATAGTTGCAGGTCAAGATCCATTATGGCTTGCTTGGGCAAATGATTTGATTAATTTAAAAGCAACCGGCAATATGA CAGAATATAATAATTTGTTAGCAACTGTTATACCTGCGCGTTTTAAAGTAGGGCAGGTAATAACATCTTCAGCTTCATTA TTAGGTGTAGCTTTTGCAATGTATAAAAATGTAGATAAAGATAAAAGAGATAAATATAAAACTGTTTTCTTATCAGCGGC ATTAGCAGTATTTTTAACAGGTGTTACAGAACCTATAGAATTTATGTTTATGTTTATATCACCAATTTTATATGGAGTTT ATGCAGTTATTACAGGAACAGCTTTTGCTTTAGCAGATTTAATAAACTTAAGAGTTCACTGTTTTGGATTCGTTGAATTT ATAGCTCGTACTCCTATGATGATAAAGGCTGGAATTACAAGAGATATGTTAAACTTTGCAATTGTATCAGTTATATATTT TGTATTAACTTATTTAATATTTAATTTTTTAATAAAGAAATTCAATATACCTACTCCAGGTAGAGCAGGAAACTATATTG AAATGGAAGGAGAAGAAGATAAAAAAGAAGAAAAAATATCAGAAAAAGATATAGATAGGGATTCATTGTCAGTGAAAATA ATAGGTTTACTAGGAGAAAAGGAAAATATAGTAGATGTAGATGCCTGCATGACTCGTCTTCGTGTAACAGTAAAGGATAA AGGCTTAGTTGCAGAGGAAAAAGAGTGGAAAAAACTTGGAGCATTAGGTCTTATAGTAAAGGATAAAGGGGTTCAGGCTA TATATGGACCAAAGGCAGATGTATTAAAATCAGATATTCAAGATATTTTAGGAGATTAA
Upstream 100 bases:
>100_bases AGGATATAACAGAAATATTATTAAAGCCTTTTGAAGCGAGACTTTATAAAATAGTTTGAAATTTAAATTAATATAGATTA TAAATATTGGGGGGATAAAA
Downstream 100 bases:
>100_bases TTTATGAAGATTCTTACTTTGAATTGTCACTCTTGGCAAGAAGAAAAACAATTAGAAAAGATAAAATATTTAGCAAAGGT GATATACGAAAATAATTATG
Product: PTS system glucose/glucoside family transporter subunit IIBC
Products: NA
Alternate protein names: EII-Glc/EIII-Glc; EIICBA-Glc; Glucose permease IIC component; PTS system glucose-specific EIIC component; Glucose-specific phosphotransferase enzyme IIB component; PTS system glucose-specific EIIB component; Glucose-specific phosphotransferase enzyme IIA component; PTS system glucose-specific EIIA component [H]
Number of amino acids: Translated: 552; Mature: 552
Protein sequence:
>552_residues MKRNKLVSFDFWQKFGKTLLVVVAVMPAAGLMICIGKLIGMQASMGLVQSVARVVEDIGWAIIGNLHILFAVAIGGSWAK ERAGGAFAGLLTFILTNRITGAIFGVKPDMFSDEAAKVTSMFGRQLVVKDYFTTILGAPALNMGVFIGIISGFLGAYLYN KYYNFNKLPKSLAFFNGKRFVPFVVILGSVVTAIILSMIWPSIQGALNAFGKWIATSKDTAPIVAPFIFGSLERILLPFG LHHMLTVPLNYTSLGGTYKILTGPTAGTIVAGQDPLWLAWANDLINLKATGNMTEYNNLLATVIPARFKVGQVITSSASL LGVAFAMYKNVDKDKRDKYKTVFLSAALAVFLTGVTEPIEFMFMFISPILYGVYAVITGTAFALADLINLRVHCFGFVEF IARTPMMIKAGITRDMLNFAIVSVIYFVLTYLIFNFLIKKFNIPTPGRAGNYIEMEGEEDKKEEKISEKDIDRDSLSVKI IGLLGEKENIVDVDACMTRLRVTVKDKGLVAEEKEWKKLGALGLIVKDKGVQAIYGPKADVLKSDIQDILGD
Sequences:
>Translated_552_residues MKRNKLVSFDFWQKFGKTLLVVVAVMPAAGLMICIGKLIGMQASMGLVQSVARVVEDIGWAIIGNLHILFAVAIGGSWAK ERAGGAFAGLLTFILTNRITGAIFGVKPDMFSDEAAKVTSMFGRQLVVKDYFTTILGAPALNMGVFIGIISGFLGAYLYN KYYNFNKLPKSLAFFNGKRFVPFVVILGSVVTAIILSMIWPSIQGALNAFGKWIATSKDTAPIVAPFIFGSLERILLPFG LHHMLTVPLNYTSLGGTYKILTGPTAGTIVAGQDPLWLAWANDLINLKATGNMTEYNNLLATVIPARFKVGQVITSSASL LGVAFAMYKNVDKDKRDKYKTVFLSAALAVFLTGVTEPIEFMFMFISPILYGVYAVITGTAFALADLINLRVHCFGFVEF IARTPMMIKAGITRDMLNFAIVSVIYFVLTYLIFNFLIKKFNIPTPGRAGNYIEMEGEEDKKEEKISEKDIDRDSLSVKI IGLLGEKENIVDVDACMTRLRVTVKDKGLVAEEKEWKKLGALGLIVKDKGVQAIYGPKADVLKSDIQDILGD >Mature_552_residues MKRNKLVSFDFWQKFGKTLLVVVAVMPAAGLMICIGKLIGMQASMGLVQSVARVVEDIGWAIIGNLHILFAVAIGGSWAK ERAGGAFAGLLTFILTNRITGAIFGVKPDMFSDEAAKVTSMFGRQLVVKDYFTTILGAPALNMGVFIGIISGFLGAYLYN KYYNFNKLPKSLAFFNGKRFVPFVVILGSVVTAIILSMIWPSIQGALNAFGKWIATSKDTAPIVAPFIFGSLERILLPFG LHHMLTVPLNYTSLGGTYKILTGPTAGTIVAGQDPLWLAWANDLINLKATGNMTEYNNLLATVIPARFKVGQVITSSASL LGVAFAMYKNVDKDKRDKYKTVFLSAALAVFLTGVTEPIEFMFMFISPILYGVYAVITGTAFALADLINLRVHCFGFVEF IARTPMMIKAGITRDMLNFAIVSVIYFVLTYLIFNFLIKKFNIPTPGRAGNYIEMEGEEDKKEEKISEKDIDRDSLSVKI IGLLGEKENIVDVDACMTRLRVTVKDKGLVAEEKEWKKLGALGLIVKDKGVQAIYGPKADVLKSDIQDILGD
Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This
COG id: COG1263
COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PTS EIIC type-1 domain [H]
Homologues:
Organism=Escherichia coli, GI1787343, Length=545, Percent_Identity=32.6605504587156, Blast_Score=252, Evalue=3e-68, Organism=Escherichia coli, GI1787908, Length=564, Percent_Identity=27.6595744680851, Blast_Score=214, Evalue=1e-56, Organism=Escherichia coli, GI1786894, Length=546, Percent_Identity=30.03663003663, Blast_Score=188, Evalue=9e-49,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011055 - InterPro: IPR018113 - InterPro: IPR001127 - InterPro: IPR001996 - InterPro: IPR003352 - InterPro: IPR013013 - InterPro: IPR011535 - InterPro: IPR011300 [H]
Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]
EC number: =2.7.1.69 [H]
Molecular weight: Translated: 60446; Mature: 60446
Theoretical pI: Translated: 9.74; Mature: 9.74
Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 3.4 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 3.4 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKRNKLVSFDFWQKFGKTLLVVVAVMPAAGLMICIGKLIGMQASMGLVQSVARVVEDIGW CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH AIIGNLHILFAVAIGGSWAKERAGGAFAGLLTFILTNRITGAIFGVKPDMFSDEAAKVTS HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHH MFGRQLVVKDYFTTILGAPALNMGVFIGIISGFLGAYLYNKYYNFNKLPKSLAFFNGKRF HHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHCCCCH VPFVVILGSVVTAIILSMIWPSIQGALNAFGKWIATSKDTAPIVAPFIFGSLERILLPFG HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC LHHMLTVPLNYTSLGGTYKILTGPTAGTIVAGQDPLWLAWANDLINLKATGNMTEYNNLL CHHHEEECCCEEECCCEEEEEECCCCCEEEECCCCEEEEECCCEEEEEECCCCHHHHHHH ATVIPARFKVGQVITSSASLLGVAFAMYKNVDKDKRDKYKTVFLSAALAVFLTGVTEPIE HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH FMFMFISPILYGVYAVITGTAFALADLINLRVHCFGFVEFIARTPMMIKAGITRDMLNFA HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCHHHHHHHH IVSVIYFVLTYLIFNFLIKKFNIPTPGRAGNYIEMEGEEDKKEEKISEKDIDRDSLSVKI HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEEE IGLLGEKENIVDVDACMTRLRVTVKDKGLVAEEKEWKKLGALGLIVKDKGVQAIYGPKAD EEEECCCCCEEEHHHHHHHHEEEECCCCCCCCHHHHHHHCCEEEEEECCCCCEEECCCHH VLKSDIQDILGD HHHHHHHHHHCC >Mature Secondary Structure MKRNKLVSFDFWQKFGKTLLVVVAVMPAAGLMICIGKLIGMQASMGLVQSVARVVEDIGW CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH AIIGNLHILFAVAIGGSWAKERAGGAFAGLLTFILTNRITGAIFGVKPDMFSDEAAKVTS HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHH MFGRQLVVKDYFTTILGAPALNMGVFIGIISGFLGAYLYNKYYNFNKLPKSLAFFNGKRF HHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHCCCCH VPFVVILGSVVTAIILSMIWPSIQGALNAFGKWIATSKDTAPIVAPFIFGSLERILLPFG HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC LHHMLTVPLNYTSLGGTYKILTGPTAGTIVAGQDPLWLAWANDLINLKATGNMTEYNNLL CHHHEEECCCEEECCCEEEEEECCCCCEEEECCCCEEEEECCCEEEEEECCCCHHHHHHH ATVIPARFKVGQVITSSASLLGVAFAMYKNVDKDKRDKYKTVFLSAALAVFLTGVTEPIE HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH FMFMFISPILYGVYAVITGTAFALADLINLRVHCFGFVEFIARTPMMIKAGITRDMLNFA HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCHHHHHHHH IVSVIYFVLTYLIFNFLIKKFNIPTPGRAGNYIEMEGEEDKKEEKISEKDIDRDSLSVKI HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEEE IGLLGEKENIVDVDACMTRLRVTVKDKGLVAEEKEWKKLGALGLIVKDKGVQAIYGPKAD EEEECCCCCEEEHHHHHHHHEEEECCCCCCCCHHHHHHHCCEEEEEECCCCCEEECCCHH VLKSDIQDILGD HHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 11463916; 7934910 [H]