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The map label for this gene is hisF [H]

Identifier: 226948903

GI number: 226948903

Start: 1844312

End: 1845073

Strand: Direct

Name: hisF [H]

Synonym: CLM_1813

Alternate gene names: 226948903

Gene position: 1844312-1845073 (Clockwise)

Preceding gene: 226948902

Following gene: 226948904

Centisome position: 44.38

GC content: 33.33

Gene sequence:

>762_bases
GTGATTACAAAGAGGATAATACCTTGCTTAGATGTAGATATGGGGAGAGTTGTAAAGGGAGTTAATTTTGTTAATCTAAA
GGATGTAGGTGATCCTGTAGAAATAGCAGAGTTTTATAACAAAGAAGGAGCAGATGAAATAGTATTTCTAGATATATCAG
CTACTCATGAAGGAAGAGCTACAATGATAGATGTGGTTAGAAAAACAGCAGAGAAACTATTTATCCCTCTTACTGTAGGA
GGGGGAATTAAAAATATAAATGATTTTAGAGATATATTAAGAGCTGGGGCAGATAAAATATCAGTTAATTCATCTGCTAT
AAGAAATCCTAAACTTATAAAAAAGGCAGCAGAATGTTTTGGATCACAATGTGTTGTAGTGGCTATAGATGGCAAAAAAC
GAAAAGATAAGGATGGATGGAATGTATTTATAAATGGAGGCAGAATAGATACAGGCATTGATGCTATAGAGTGGGCAAGA
AAGGTTGAGAAATTAGGAGCCGGTGAGATATTATTAACAAGTATGGATGCAGATGGTACAAAAGAAGGTTATGATTTAGA
GTTTACAAATGAAGTTTCAAAGGCAGTAAATATTCCTGTAATTGCTTCAGGAGGATGCGGAAAACTGAAACATTTTGGAG
AAATTTTTGAAAAAAGTTCAGCAGATGCAGCATTAGCAGCTTCGTTATTTCATTTTAAAGAATTGAGCATAAAGGAAGTT
AAAAACTATTTAAAAAAAGAAGGCTTTAGTGTAAGACTTTAG

Upstream 100 bases:

>100_bases
AAAAATCGGGTAAAAGTATTATTGAAGTATAAAATTATGAAAAAAGAATATATGACTAAAAAATAGAAGTTTAAAAATAT
GCTAAAAAGGGGGAGTATAT

Downstream 100 bases:

>100_bases
TTTTTTATATTTGAATTATTAAGATTTTATAGTTAAGTTCCACTCTATATAAAGATTATAAATGATTACAAATATATATT
GTAACTCTCATCTGCATATT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MITKRIIPCLDVDMGRVVKGVNFVNLKDVGDPVEIAEFYNKEGADEIVFLDISATHEGRATMIDVVRKTAEKLFIPLTVG
GGIKNINDFRDILRAGADKISVNSSAIRNPKLIKKAAECFGSQCVVVAIDGKKRKDKDGWNVFINGGRIDTGIDAIEWAR
KVEKLGAGEILLTSMDADGTKEGYDLEFTNEVSKAVNIPVIASGGCGKLKHFGEIFEKSSADAALAASLFHFKELSIKEV
KNYLKKEGFSVRL

Sequences:

>Translated_253_residues
MITKRIIPCLDVDMGRVVKGVNFVNLKDVGDPVEIAEFYNKEGADEIVFLDISATHEGRATMIDVVRKTAEKLFIPLTVG
GGIKNINDFRDILRAGADKISVNSSAIRNPKLIKKAAECFGSQCVVVAIDGKKRKDKDGWNVFINGGRIDTGIDAIEWAR
KVEKLGAGEILLTSMDADGTKEGYDLEFTNEVSKAVNIPVIASGGCGKLKHFGEIFEKSSADAALAASLFHFKELSIKEV
KNYLKKEGFSVRL
>Mature_253_residues
MITKRIIPCLDVDMGRVVKGVNFVNLKDVGDPVEIAEFYNKEGADEIVFLDISATHEGRATMIDVVRKTAEKLFIPLTVG
GGIKNINDFRDILRAGADKISVNSSAIRNPKLIKKAAECFGSQCVVVAIDGKKRKDKDGWNVFINGGRIDTGIDAIEWAR
KVEKLGAGEILLTSMDADGTKEGYDLEFTNEVSKAVNIPVIASGGCGKLKHFGEIFEKSSADAALAASLFHFKELSIKEV
KNYLKKEGFSVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=45.3488372093023, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082028, Length=209, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.031847133758, Blast_Score=169, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27610; Mature: 27610

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKRIIPCLDVDMGRVVKGVNFVNLKDVGDPVEIAEFYNKEGADEIVFLDISATHEGRA
CCCCCCCCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCH
TMIDVVRKTAEKLFIPLTVGGGIKNINDFRDILRAGADKISVNSSAIRNPKLIKKAAECF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHCCHHHHHHHHHHC
GSQCVVVAIDGKKRKDKDGWNVFINGGRIDTGIDAIEWARKVEKLGAGEILLTSMDADGT
CCCEEEEEECCCCCCCCCCCEEEEECCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
KEGYDLEFTNEVSKAVNIPVIASGGCGKLKHFGEIFEKSSADAALAASLFHFKELSIKEV
CCCCCEEEHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHH
KNYLKKEGFSVRL
HHHHHHCCCEECC
>Mature Secondary Structure
MITKRIIPCLDVDMGRVVKGVNFVNLKDVGDPVEIAEFYNKEGADEIVFLDISATHEGRA
CCCCCCCCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCH
TMIDVVRKTAEKLFIPLTVGGGIKNINDFRDILRAGADKISVNSSAIRNPKLIKKAAECF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHCCHHHHHHHHHHC
GSQCVVVAIDGKKRKDKDGWNVFINGGRIDTGIDAIEWARKVEKLGAGEILLTSMDADGT
CCCEEEEEECCCCCCCCCCCEEEEECCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
KEGYDLEFTNEVSKAVNIPVIASGGCGKLKHFGEIFEKSSADAALAASLFHFKELSIKEV
CCCCCEEEHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHH
KNYLKKEGFSVRL
HHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA