Click here to switch to the map view.

The map label for this gene is fadH [C]

Identifier: 226948837

GI number: 226948837

Start: 1773331

End: 1774350

Strand: Direct

Name: fadH [C]

Synonym: CLM_1739

Alternate gene names: 226948837

Gene position: 1773331-1774350 (Clockwise)

Preceding gene: 226948835

Following gene: 226948838

Centisome position: 42.68

GC content: 30.59

Gene sequence:

>1020_bases
ATGAAAAGTTTATTTGATAAAACCTGCATAAAAACAATGAAACTTAAAAATAGATTTGTTCGCTCAGCCACCTGGGAGGG
CATGGGCACAGAAGAAGGACATATTACGGAGAGATTACTTAATTTGTATGAAAAATTAGCAAAGGGTGGAGTTGGATTAA
TAATAAGTAGTTACACTACTATATTTGATTATGATAAGCCAAGTCTTAGAATCCTTGGAATTTATGATGATAGCTTTATT
GAAGAATATAAGCTTTTGACAGATATAATTCATAAATATGAGGCTAAAGTATTAATGCAAATAGTCTTAGGGGAGAATTA
TATTAATAATGAAACAGGTAGTGAATTTTACGGATTAAGCGAAAATATGCCAGAAGATGATATAAAGACTATAGTAAAAT
CTTTTGCAAAGGCAGCTAAAAGGGCTAAAGAGTCAGGTTTTGATGGAATTCAAATTCATGGAGCCCATGGATATTTTTTA
AGTAGAACATTAAGTCCTCTTTTTAATAAAAGAAAGGATAAATACGGTGGTTCAGTAGAAAAAAGAGGTGCACTAATACT
AGAGGTTTATGATGAGATAAGAAAAGCAGTGGGAAAAGATTTTCATATATCCATAAAAATTAATTGTTCTGATTTTGAAG
AGGGCGGAGCAACCTTTAAGGAATGTGAATTTGTTTGCAGGGAACTCTCTAAGAAAGGTATAGATTCTATAGAAATCAGC
GGTGGAGGAACAATTTGGACAGAAACTAATAAAAAGGAATCTATATATAAAGAGTATGCCTCAAAAATAGCTGAAGAAGT
AGATACTCCCATAATTTTAGTTGGTATGAATAGAAGTTATGACAATATGAATGAAATATTAAATAATAGTAAGATAGAAT
ATTTTTCAATGGCTAGACCTTTTATAAGGGAACCGGATTTAATAAATAAATTTGAAAAAGGTGAAAATAAAAAAGCTAAA
TGTATATCCTGTGGGAAATGTTGTGGTGAAAATGGAATAAGGTGTATATTCAATATGTAA

Upstream 100 bases:

>100_bases
GTTAAAAATTCTGTTTTAATTCCTTTGGTGAAAGTTCAACATTAAATCAAATTATGCTAATATAATAATATAGAATTGAA
ACAAAACAGGGGGAACTTTT

Downstream 100 bases:

>100_bases
AATAAATATAAGGATGGGGAATGTAAGTAAAATTCTCCATCCTTATATTTATGTAAGCATTATTTTAAGATATATCAAAT
TAGTATTTTAAAAACAAAAG

Product: FAD/FMN-binding family oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKSLFDKTCIKTMKLKNRFVRSATWEGMGTEEGHITERLLNLYEKLAKGGVGLIISSYTTIFDYDKPSLRILGIYDDSFI
EEYKLLTDIIHKYEAKVLMQIVLGENYINNETGSEFYGLSENMPEDDIKTIVKSFAKAAKRAKESGFDGIQIHGAHGYFL
SRTLSPLFNKRKDKYGGSVEKRGALILEVYDEIRKAVGKDFHISIKINCSDFEEGGATFKECEFVCRELSKKGIDSIEIS
GGGTIWTETNKKESIYKEYASKIAEEVDTPIILVGMNRSYDNMNEILNNSKIEYFSMARPFIREPDLINKFEKGENKKAK
CISCGKCCGENGIRCIFNM

Sequences:

>Translated_339_residues
MKSLFDKTCIKTMKLKNRFVRSATWEGMGTEEGHITERLLNLYEKLAKGGVGLIISSYTTIFDYDKPSLRILGIYDDSFI
EEYKLLTDIIHKYEAKVLMQIVLGENYINNETGSEFYGLSENMPEDDIKTIVKSFAKAAKRAKESGFDGIQIHGAHGYFL
SRTLSPLFNKRKDKYGGSVEKRGALILEVYDEIRKAVGKDFHISIKINCSDFEEGGATFKECEFVCRELSKKGIDSIEIS
GGGTIWTETNKKESIYKEYASKIAEEVDTPIILVGMNRSYDNMNEILNNSKIEYFSMARPFIREPDLINKFEKGENKKAK
CISCGKCCGENGIRCIFNM
>Mature_339_residues
MKSLFDKTCIKTMKLKNRFVRSATWEGMGTEEGHITERLLNLYEKLAKGGVGLIISSYTTIFDYDKPSLRILGIYDDSFI
EEYKLLTDIIHKYEAKVLMQIVLGENYINNETGSEFYGLSENMPEDDIKTIVKSFAKAAKRAKESGFDGIQIHGAHGYFL
SRTLSPLFNKRKDKYGGSVEKRGALILEVYDEIRKAVGKDFHISIKINCSDFEEGGATFKECEFVCRELSKKGIDSIEIS
GGGTIWTETNKKESIYKEYASKIAEEVDTPIILVGMNRSYDNMNEILNNSKIEYFSMARPFIREPDLINKFEKGENKKAK
CISCGKCCGENGIRCIFNM

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=351, Percent_Identity=26.7806267806268, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787939, Length=347, Percent_Identity=24.4956772334294, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17540738, Length=348, Percent_Identity=29.5977011494253, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17564188, Length=339, Percent_Identity=31.5634218289086, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17559804, Length=345, Percent_Identity=31.5942028985507, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17559802, Length=340, Percent_Identity=31.4705882352941, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI72001454, Length=345, Percent_Identity=30.1449275362319, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17565138, Length=333, Percent_Identity=29.4294294294294, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17566914, Length=337, Percent_Identity=28.7833827893175, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325086, Length=371, Percent_Identity=23.989218328841, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=225, Percent_Identity=27.1111111111111, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 38499; Mature: 38499

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLFDKTCIKTMKLKNRFVRSATWEGMGTEEGHITERLLNLYEKLAKGGVGLIISSYTT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
IFDYDKPSLRILGIYDDSFIEEYKLLTDIIHKYEAKVLMQIVLGENYINNETGSEFYGLS
EEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ENMPEDDIKTIVKSFAKAAKRAKESGFDGIQIHGAHGYFLSRTLSPLFNKRKDKYGGSVE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHH
KRGALILEVYDEIRKAVGKDFHISIKINCSDFEEGGATFKECEFVCRELSKKGIDSIEIS
HCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
GGGTIWTETNKKESIYKEYASKIAEEVDTPIILVGMNRSYDNMNEILNNSKIEYFSMARP
CCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCHHHHHCCC
FIREPDLINKFEKGENKKAKCISCGKCCGENGIRCIFNM
CCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MKSLFDKTCIKTMKLKNRFVRSATWEGMGTEEGHITERLLNLYEKLAKGGVGLIISSYTT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
IFDYDKPSLRILGIYDDSFIEEYKLLTDIIHKYEAKVLMQIVLGENYINNETGSEFYGLS
EEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ENMPEDDIKTIVKSFAKAAKRAKESGFDGIQIHGAHGYFLSRTLSPLFNKRKDKYGGSVE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHH
KRGALILEVYDEIRKAVGKDFHISIKINCSDFEEGGATFKECEFVCRELSKKGIDSIEIS
HCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
GGGTIWTETNKKESIYKEYASKIAEEVDTPIILVGMNRSYDNMNEILNNSKIEYFSMARP
CCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCHHHHHCCC
FIREPDLINKFEKGENKKAKCISCGKCCGENGIRCIFNM
CCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]