| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
Click here to switch to the map view.
The map label for this gene is murF [H]
Identifier: 226948794
GI number: 226948794
Start: 1731930
End: 1733318
Strand: Direct
Name: murF [H]
Synonym: CLM_1695
Alternate gene names: 226948794
Gene position: 1731930-1733318 (Clockwise)
Preceding gene: 226948793
Following gene: 226948795
Centisome position: 41.68
GC content: 28.44
Gene sequence:
>1389_bases ATGGAAAATATTAAACTAGAAGAAATAATAAAAGCAGTTAATGGTGAGTTAGTGATAACTGGAGAAAAAGATGAATATAA TTCAGTAAGCACAGATACAAGAAAAATAGAAAAGGGAGATATATTTATAGCCTTAAAAGGAGAAAATTTTAATGGAAATA ACTTTGTAGAAATGGCCATAGAAAAGGGTGCCGCTTTATGCATAGTAAGTGAAATAAATTTTAGCGAAGGAAAAGTGGGC AAATCTTCCTATGTAATAAAGGTAGAGAATACTAATAAGGCTCTTTTAGATTTAGCTAAATACTATAGAAGTAAACTAGA TATAAAAGTGGTTGCTATTACTGGATCTACAGGAAAAACATCTACAAAGGATTTAGTGGCAGCTGTGCTTTCAGAAAAAT ATAAGGTATTTAAAACAGAGGGAAATTTTAATAATGAGATAGGACTTCCTCTTATGATCTTTAAATTAGATAAAAGTTAT GATATAGCAGTTTTAGAAATGGGGATGAATCATTTTAATGAAATACACAATATGGCAGAAGCAGCAAAACCAGATATAGC TATAATAACTAATATAGGTATATCTCATATAGAAAATTTAGGATCTAGGGAAAATATTTTAAAAGCAAAACTAGAAATAA CAGATTTCTTCTGTAAGGATAGTATTTTAATAATAAATGGAGACGATGATCTTCTATCAAATTTTGAAAGTGATAAATTT AAGGTATACAAAATAGGCACAGATAAAAAATTTGATTTTAATGGAGATAATTTAAATTTAGAGGCAGAAAGTATAGAATT TGATATATTAGAACAAGGTAAAACAGCTTATAAAGGTTTTAGCGTAAATGTGCCTGGAAAGCATAATGTGCTTAATTCTC TTACAGCTATAGCTTGTGGAAGAGTTTTAGATATAAATTACAGGGATATACAAACTGGGATAAAAAATTTAAAAGCTACT TCTATGAGATTAGATATTGTTAAGGAAAAGGGATTTACTATAATAAATGATTGTTATAATGCAAGTCCCGATTCTATGAA AGCAGCTATAGATGTAATGAAAAATATAAAGGGAAAAAGGGCTATAGCTTTATTAGGTTCTATGAGGGAACTTGGATCCG AAGGGTATAGAGCTCATAGGGAAGTTTCTGAATATGCAAGAGAAAAGGGAATAGATCTATTATTTTCCATAGGAGAATTT AGTGAAGCATATAGAGAAGGGTTTGAAGAAATAAACAAAGAGAATTATAAAAGCTTTTTAAATAATGAAGAAGCAGCAAA ATACATAAAAAATATAATAAAAGATGGTGATATAATATTAATTAAAGCATCTAGAGCTATGAAGCTAGAAGAAATAGTAA AAGAACTAAGGATAAAACAAGAAAAATAG
Upstream 100 bases:
>100_bases TATCAAATATTAAAGGATAAAACTATTCACTTTGATGAAAGAGAAGTAATAGAAGAACTAATAAAAGAAATATAATTACT CTAAAAATGGGAGTGTTAAC
Downstream 100 bases:
>100_bases GAGGTGACTTGCTCACATAATTAGAGCAATTATTTATGAATAGAATAGTATATGCAGTGCTTTTAGCCTTTTTAATATCA ATATTAGGAGGACCAATATT
Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 462; Mature: 462
Protein sequence:
>462_residues MENIKLEEIIKAVNGELVITGEKDEYNSVSTDTRKIEKGDIFIALKGENFNGNNFVEMAIEKGAALCIVSEINFSEGKVG KSSYVIKVENTNKALLDLAKYYRSKLDIKVVAITGSTGKTSTKDLVAAVLSEKYKVFKTEGNFNNEIGLPLMIFKLDKSY DIAVLEMGMNHFNEIHNMAEAAKPDIAIITNIGISHIENLGSRENILKAKLEITDFFCKDSILIINGDDDLLSNFESDKF KVYKIGTDKKFDFNGDNLNLEAESIEFDILEQGKTAYKGFSVNVPGKHNVLNSLTAIACGRVLDINYRDIQTGIKNLKAT SMRLDIVKEKGFTIINDCYNASPDSMKAAIDVMKNIKGKRAIALLGSMRELGSEGYRAHREVSEYAREKGIDLLFSIGEF SEAYREGFEEINKENYKSFLNNEEAAKYIKNIIKDGDIILIKASRAMKLEEIVKELRIKQEK
Sequences:
>Translated_462_residues MENIKLEEIIKAVNGELVITGEKDEYNSVSTDTRKIEKGDIFIALKGENFNGNNFVEMAIEKGAALCIVSEINFSEGKVG KSSYVIKVENTNKALLDLAKYYRSKLDIKVVAITGSTGKTSTKDLVAAVLSEKYKVFKTEGNFNNEIGLPLMIFKLDKSY DIAVLEMGMNHFNEIHNMAEAAKPDIAIITNIGISHIENLGSRENILKAKLEITDFFCKDSILIINGDDDLLSNFESDKF KVYKIGTDKKFDFNGDNLNLEAESIEFDILEQGKTAYKGFSVNVPGKHNVLNSLTAIACGRVLDINYRDIQTGIKNLKAT SMRLDIVKEKGFTIINDCYNASPDSMKAAIDVMKNIKGKRAIALLGSMRELGSEGYRAHREVSEYAREKGIDLLFSIGEF SEAYREGFEEINKENYKSFLNNEEAAKYIKNIIKDGDIILIKASRAMKLEEIVKELRIKQEK >Mature_462_residues MENIKLEEIIKAVNGELVITGEKDEYNSVSTDTRKIEKGDIFIALKGENFNGNNFVEMAIEKGAALCIVSEINFSEGKVG KSSYVIKVENTNKALLDLAKYYRSKLDIKVVAITGSTGKTSTKDLVAAVLSEKYKVFKTEGNFNNEIGLPLMIFKLDKSY DIAVLEMGMNHFNEIHNMAEAAKPDIAIITNIGISHIENLGSRENILKAKLEITDFFCKDSILIINGDDDLLSNFESDKF KVYKIGTDKKFDFNGDNLNLEAESIEFDILEQGKTAYKGFSVNVPGKHNVLNSLTAIACGRVLDINYRDIQTGIKNLKAT SMRLDIVKEKGFTIINDCYNASPDSMKAAIDVMKNIKGKRAIALLGSMRELGSEGYRAHREVSEYAREKGIDLLFSIGEF SEAYREGFEEINKENYKSFLNNEEAAKYIKNIIKDGDIILIKASRAMKLEEIVKELRIKQEK
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=472, Percent_Identity=32.4152542372881, Blast_Score=230, Evalue=2e-61,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 51744; Mature: 51744
Theoretical pI: Translated: 5.49; Mature: 5.49
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MENIKLEEIIKAVNGELVITGEKDEYNSVSTDTRKIEKGDIFIALKGENFNGNNFVEMAI CCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCHHEEECCCEEEEEECCCCCCCCEEEEEE EKGAALCIVSEINFSEGKVGKSSYVIKVENTNKALLDLAKYYRSKLDIKVVAITGSTGKT CCCCEEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCC STKDLVAAVLSEKYKVFKTEGNFNNEIGLPLMIFKLDKSYDIAVLEMGMNHFNEIHNMAE CHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEHHCHHHHHHHHHHHH AAKPDIAIITNIGISHIENLGSRENILKAKLEITDFFCKDSILIINGDDDLLSNFESDKF HCCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHEEEEECCCEEEEECCHHHHHCCCCCCE KVYKIGTDKKFDFNGDNLNLEAESIEFDILEQGKTAYKGFSVNVPGKHNVLNSLTAIACG EEEEECCCCEECCCCCCEEEECCCEEEEEHHCCCHHHCCEEECCCCCHHHHHHHHHHHCC RVLDINYRDIQTGIKNLKATSMRLDIVKEKGFTIINDCYNASPDSMKAAIDVMKNIKGKR CEEECCHHHHHHHHHHHHHHHEEEEEECCCCCEEEEHHCCCCCHHHHHHHHHHHHCCCCH AIALLGSMRELGSEGYRAHREVSEYAREKGIDLLFSIGEFSEAYREGFEEINKENYKSFL HHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCHHHHHHHH NNEEAAKYIKNIIKDGDIILIKASRAMKLEEIVKELRIKQEK CCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCC >Mature Secondary Structure MENIKLEEIIKAVNGELVITGEKDEYNSVSTDTRKIEKGDIFIALKGENFNGNNFVEMAI CCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCHHEEECCCEEEEEECCCCCCCCEEEEEE EKGAALCIVSEINFSEGKVGKSSYVIKVENTNKALLDLAKYYRSKLDIKVVAITGSTGKT CCCCEEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCC STKDLVAAVLSEKYKVFKTEGNFNNEIGLPLMIFKLDKSYDIAVLEMGMNHFNEIHNMAE CHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEHHCHHHHHHHHHHHH AAKPDIAIITNIGISHIENLGSRENILKAKLEITDFFCKDSILIINGDDDLLSNFESDKF HCCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHEEEEECCCEEEEECCHHHHHCCCCCCE KVYKIGTDKKFDFNGDNLNLEAESIEFDILEQGKTAYKGFSVNVPGKHNVLNSLTAIACG EEEEECCCCEECCCCCCEEEECCCEEEEEHHCCCHHHCCEEECCCCCHHHHHHHHHHHCC RVLDINYRDIQTGIKNLKATSMRLDIVKEKGFTIINDCYNASPDSMKAAIDVMKNIKGKR CEEECCHHHHHHHHHHHHHHHEEEEEECCCCCEEEEHHCCCCCHHHHHHHHHHHHCCCCH AIALLGSMRELGSEGYRAHREVSEYAREKGIDLLFSIGEFSEAYREGFEEINKENYKSFL HHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCHHHHHHHH NNEEAAKYIKNIIKDGDIILIKASRAMKLEEIVKELRIKQEK CCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]