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The map label for this gene is nadB [H]

Identifier: 226948711

GI number: 226948711

Start: 1682456

End: 1683757

Strand: Reverse

Name: nadB [H]

Synonym: CLM_1611

Alternate gene names: 226948711

Gene position: 1683757-1682456 (Counterclockwise)

Preceding gene: 226948712

Following gene: 226948710

Centisome position: 40.52

GC content: 28.11

Gene sequence:

>1302_bases
ATGAATCTTTATGCTGATGTCTTGATTGCAGGAAGCGGCGTAGCTGGATTATATTCAGCTTTAAATCTAAGAAAAGACTT
AAAAATAGTATTAATCTCAAAAGGTAAACTTAACCAATGCAATACTACCCTAGCCCAGGGTGGAATCTCTGTAGCAAGAA
ATAAGGATGATATAACCGCTTTTGTAGAAGATACTTTAAAAGCGGGCTCCTATGAAAATAACTTGTGTGCAGTAAAAATA
TTAGCAAATGAATCTATGGAAAACATTAATAAATTACAGGATATGGGCCTTAACTTGGATAAATGTGAAAAGGAATTAAG
CTATACAAAAGAAGGAGCTCATAGTATAAACAGAATTGTTCATTTTAAAGATTATACTGGTAAAAAATTAGAAGAAATCT
TAACTAAGAAAACACTTAAAAGAGAAAATATAACTGTAATCGAAAATTGTAGCCTTGTAGATTTAATACAAAATAATAAT
ACTTGCTTTGGAGGTATATGTTTAAAAAATAATAAGCAAATAAACATATATTCAAAAGTAACAATACTGGCAACAGGAGG
TATAGGAGGATTATTTAAAAACTCAACTAATGAACCAATTATAACTGGAGATGGTATAGCTATAGCTATAAAAAACAATA
TAAAAATTAAAAATTTGGATTATATACAATTTCATCCTACAGCATTTTTTAAGAATACTATTAAGGGAAAAAAATTTTTA
ATTTCTGAGTCAGTACGAGGAGAAGGAGGAAAGCTTTTAAATTCTAAAGGTGAAAGATTTGTAAATGAATTACTCTCTAG
AGATATAGTAACCAAATATATCCTAGAAGAGGAAATAAAAACCAGATCTAATAATGTATATTTAGATATCACCTTTAAAC
CTGAAACTTTTTTAAAGAAAAGATTCCCTACTATTTATGAAAATTGTTTAAAAAATGGCGTAAATATTTGTAAAGATGCT
ATTCCTGTATCTCCAGCACAACATTATTTCATGGGTGGAATACAAGTAGACTTATTGGGACAATCTTCTATGAATAATTT
ATATGCTTTTGGTGAAGTAAGTTGCACTGGAGTACACGGTAAAAATAGACTTGCTAGCAACTCCCTACTAGAAGGGCTAG
TATTTTCAAAACGCGGAGCTGAAAAAATAAATTTTGAAATAAATAATATTAGATTATCATATACAAAGATTTATACATTG
AAACATAATGTAGAATATTATAGTTTATTAAATAAAAAAATAATTACAGAATCTCTACTTAAACTAAGGAGTGATTTACA
AAATGAATTGGTTAGTTGTTGA

Upstream 100 bases:

>100_bases
GTCTTTTAAATTTAAATTATAAAATAGAATTAGATAAAAATTTAAGATTAAAGGCCTATGATGCCCTTATAAATATGCAT
ACTTTAGGAGGCAGATAACC

Downstream 100 bases:

>100_bases
TGAAATACTTAAATCTGCACTTAAAGAAGATATATCCTTTGAAGACATAACTACAGAATCTATATTTAAAGAAAATAAAA
GAGCAAAAATAGATTTAATT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNQCNTTLAQGGISVARNKDDITAFVEDTLKAGSYENNLCAVKI
LANESMENINKLQDMGLNLDKCEKELSYTKEGAHSINRIVHFKDYTGKKLEEILTKKTLKRENITVIENCSLVDLIQNNN
TCFGGICLKNNKQINIYSKVTILATGGIGGLFKNSTNEPIITGDGIAIAIKNNIKIKNLDYIQFHPTAFFKNTIKGKKFL
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEEIKTRSNNVYLDITFKPETFLKKRFPTIYENCLKNGVNICKDA
IPVSPAQHYFMGGIQVDLLGQSSMNNLYAFGEVSCTGVHGKNRLASNSLLEGLVFSKRGAEKINFEINNIRLSYTKIYTL
KHNVEYYSLLNKKIITESLLKLRSDLQNELVSC

Sequences:

>Translated_433_residues
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNQCNTTLAQGGISVARNKDDITAFVEDTLKAGSYENNLCAVKI
LANESMENINKLQDMGLNLDKCEKELSYTKEGAHSINRIVHFKDYTGKKLEEILTKKTLKRENITVIENCSLVDLIQNNN
TCFGGICLKNNKQINIYSKVTILATGGIGGLFKNSTNEPIITGDGIAIAIKNNIKIKNLDYIQFHPTAFFKNTIKGKKFL
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEEIKTRSNNVYLDITFKPETFLKKRFPTIYENCLKNGVNICKDA
IPVSPAQHYFMGGIQVDLLGQSSMNNLYAFGEVSCTGVHGKNRLASNSLLEGLVFSKRGAEKINFEINNIRLSYTKIYTL
KHNVEYYSLLNKKIITESLLKLRSDLQNELVSC
>Mature_433_residues
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNQCNTTLAQGGISVARNKDDITAFVEDTLKAGSYENNLCAVKI
LANESMENINKLQDMGLNLDKCEKELSYTKEGAHSINRIVHFKDYTGKKLEEILTKKTLKRENITVIENCSLVDLIQNNN
TCFGGICLKNNKQINIYSKVTILATGGIGGLFKNSTNEPIITGDGIAIAIKNNIKIKNLDYIQFHPTAFFKNTIKGKKFL
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEEIKTRSNNVYLDITFKPETFLKKRFPTIYENCLKNGVNICKDA
IPVSPAQHYFMGGIQVDLLGQSSMNNLYAFGEVSCTGVHGKNRLASNSLLEGLVFSKRGAEKINFEINNIRLSYTKIYTL
KHNVEYYSLLNKKIITESLLKLRSDLQNELVSC

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=379, Percent_Identity=31.6622691292876, Blast_Score=179, Evalue=6e-45,
Organism=Escherichia coli, GI1788928, Length=399, Percent_Identity=39.3483709273183, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1786942, Length=386, Percent_Identity=31.3471502590674, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1790597, Length=384, Percent_Identity=28.90625, Blast_Score=168, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17550100, Length=388, Percent_Identity=31.701030927835, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17505833, Length=388, Percent_Identity=30.6701030927835, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71986328, Length=417, Percent_Identity=30.6954436450839, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322701, Length=396, Percent_Identity=31.3131313131313, Blast_Score=169, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6322416, Length=395, Percent_Identity=30.6329113924051, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320788, Length=479, Percent_Identity=26.5135699373695, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322511, Length=306, Percent_Identity=28.7581699346405, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137288, Length=449, Percent_Identity=29.6213808463252, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24655642, Length=449, Percent_Identity=29.6213808463252, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24655647, Length=449, Percent_Identity=29.6213808463252, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24663005, Length=393, Percent_Identity=29.2620865139949, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027 [H]

Pfam domain/function: PF00890 FAD_binding_2 [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 48325; Mature: 48325

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNQCNTTLAQGGISVARNKDDITA
CCEEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCEEECCCCHHHH
FVEDTLKAGSYENNLCAVKILANESMENINKLQDMGLNLDKCEKELSYTKEGAHSINRIV
HHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEE
HFKDYTGKKLEEILTKKTLKRENITVIENCSLVDLIQNNNTCFGGICLKNNKQINIYSKV
EECCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCEEEEEEEECCCCEEEEEEEE
TILATGGIGGLFKNSTNEPIITGDGIAIAIKNNIKIKNLDYIQFHPTAFFKNTIKGKKFL
EEEEECCCCCCCCCCCCCCEEECCCEEEEEECCEEEECCEEEEECCHHHHHHCCCCCEEE
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEEIKTRSNNVYLDITFKPETFLKK
EECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHH
RFPTIYENCLKNGVNICKDAIPVSPAQHYFMGGIQVDLLGQSSMNNLYAFGEVSCTGVHG
CCHHHHHHHHHCCHHHHHHCCCCCCCHHHEECCEEEEEECCCCCCCEEEECCEEEEECCC
KNRLASNSLLEGLVFSKRGAEKINFEINNIRLSYTKIYTLKHNVEYYSLLNKKIITESLL
CCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHH
KLRSDLQNELVSC
HHHHHHHHHHHCC
>Mature Secondary Structure
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNQCNTTLAQGGISVARNKDDITA
CCEEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCEEECCCCHHHH
FVEDTLKAGSYENNLCAVKILANESMENINKLQDMGLNLDKCEKELSYTKEGAHSINRIV
HHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEE
HFKDYTGKKLEEILTKKTLKRENITVIENCSLVDLIQNNNTCFGGICLKNNKQINIYSKV
EECCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCEEEEEEEECCCCEEEEEEEE
TILATGGIGGLFKNSTNEPIITGDGIAIAIKNNIKIKNLDYIQFHPTAFFKNTIKGKKFL
EEEEECCCCCCCCCCCCCCEEECCCEEEEEECCEEEECCEEEEECCHHHHHHCCCCCEEE
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEEIKTRSNNVYLDITFKPETFLKK
EECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHH
RFPTIYENCLKNGVNICKDAIPVSPAQHYFMGGIQVDLLGQSSMNNLYAFGEVSCTGVHG
CCHHHHHHHHHCCHHHHHHCCCCCCCHHHEECCEEEEEECCCCCCCEEEECCEEEEECCC
KNRLASNSLLEGLVFSKRGAEKINFEINNIRLSYTKIYTLKHNVEYYSLLNKKIITESLL
CCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHH
KLRSDLQNELVSC
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]