| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is npr [H]
Identifier: 226948708
GI number: 226948708
Start: 1677431
End: 1679176
Strand: Reverse
Name: npr [H]
Synonym: CLM_1608
Alternate gene names: 226948708
Gene position: 1679176-1677431 (Counterclockwise)
Preceding gene: 226948709
Following gene: 226948707
Centisome position: 40.41
GC content: 30.3
Gene sequence:
>1746_bases ATGAAAAGTAAAAAATTATTAGCTACAGTGCTAAGTGCTGTGATCACTTTTTCTACTGTTTCTGCAGTTTATGCTGCGCC TGTAGGAAAAGAAAGTAAAGTTGAACCAAAAACTACAACAATAACTTGGGAAAAAAATGAACAAAATACTAAAAAAGCTG CTACTGATATAACTGAAAAGAAATTTAACAATTCTGAGGAGATAACTAAATTCTTTGAAAAAAATATATCTAAATTTGGT GTACAAAAAGGTTCTCTTAAAAACACCAAGACTGTAAAAGACGAAAAAGGTAAAACTAACTATCATATGATTTATGAAGT AGAAGGTATACCTGTATACTATGGAAGAATTGTTTTTACAACTGAAAAAGACTCCTCCATGGATTCTATAAACGGTAGAA TTGATACTGTTTTTGAAAATGGGAATTGGAAAAACAGAATTAAACTATCAAAGGATAATGCTATAGCAAAAGCTAAAAAT AATATTAAAGATGAAAAAGCAACTAGTAAAAAGACTGATTTATATCTGTATAATTTTGAGGGCAAACCTTATGTAGTTTA TTTAGTAGATCTAATTACAGACAACGGGAGTTGGACGATTTTCGTTAATGCCGAGGATGGTTCTATAGTAAATAAATTTA ATAATACTCCTACTTTAATCGATACTAAAGGTCAAAAATTACCCAATGCTAAAAAAATTAAAGATGAAGCTAAAAAAGCT AGTAATGAAAATAGTGTAATTGATGTTCAAGGTCAAAGCGTTAAAGGAGTAGGAAGAACTAGTTTGGATGGACTAGTAAA TATTGATCTAACTTATGGCAATGGAAGATACTATTTAAAAGATAGCAATAAAAATATTTATCTATATGACTTAAAAAATC AAGTTGACGAATATGATCTATACAATTATCTTAGCAGACCTAACTATAAACAAATATTAATGAGCAAATCTGAATTAATA TCTAATTACAATAATAATTTTATAGCCGACAATCAGGTTAATTCTGTAGATGCTTATGTAAACACAAATAAAACCTATGA TTATTATAAAAACAAATTAAATAGAAACAGTATTGATAATAAGGGTATGAATATTAATGGATTTGTTCATGTAGGTAGAA ATTATGGTAATGCCTTTTGGTACGGTCCATATGATGGGATGTTCTTTGGCGATGGTGACGGAGTATACTTCTCTTCCCTT GCAAAATCTTTAGATGTTGTAGGCCACGAATTAAGTCATGGTGTAACAAATAAAGAGTCTAATCTTAAATATGAAAATGA ATCTGGTGCCCTAAATGAATCTTTCTCAGATATTATGGGAGTAGCTGTTGAAGGTAAAAACTTTGTACTAGGTGAAGATT GCTGGGTTGCTGGAGGAGTAATGAGGGATATGGAAGATCCATCCAGAGGAGGCCAACCAGCTCATATGAAAGATTATAAA TACAAACCTATGAATGATGATAACGGTGGTGTTCATACAAATTCAGGTATAATAAACCATGCTGCTTATTTAGTTGCAGA TGGAATAGAAAAAACTGGTGCAAAAAATAGTAAAGATATTATGGGAAAAATATTCTATACAGCTAATTGCTATAAATGGG ATGAAACAACAAATTTCGCTAAGTGCAGAAATGACGTAGTCCAAGTTACTAAAGAACTTTATGGCGAAAATAGTAAATAT GTTAAAATTGTTGAAAAAGCTTTTGACCAAGTTGGAATAACTGCTACACCTCAATTACCATTATAA
Upstream 100 bases:
>100_bases TAAAAATTTTTATATAAAATTATAAACCTATTAATATTTTACTATATAAATATTAATAGGTTTATAAGTAAAAATATGCT ATACTGGGGGTTATTTATTT
Downstream 100 bases:
>100_bases TAAAAGCCCTATTGAAGCTATAAATATATCTATATAAAATTATAAACCTATTGATATTTTCCTATATAAATATCAATAGG TTTATAAGTAAAAATATATT
Product: thermolysin metallopeptidase
Products: NA
Alternate protein names: Neutral protease [H]
Number of amino acids: Translated: 581; Mature: 581
Protein sequence:
>581_residues MKSKKLLATVLSAVITFSTVSAVYAAPVGKESKVEPKTTTITWEKNEQNTKKAATDITEKKFNNSEEITKFFEKNISKFG VQKGSLKNTKTVKDEKGKTNYHMIYEVEGIPVYYGRIVFTTEKDSSMDSINGRIDTVFENGNWKNRIKLSKDNAIAKAKN NIKDEKATSKKTDLYLYNFEGKPYVVYLVDLITDNGSWTIFVNAEDGSIVNKFNNTPTLIDTKGQKLPNAKKIKDEAKKA SNENSVIDVQGQSVKGVGRTSLDGLVNIDLTYGNGRYYLKDSNKNIYLYDLKNQVDEYDLYNYLSRPNYKQILMSKSELI SNYNNNFIADNQVNSVDAYVNTNKTYDYYKNKLNRNSIDNKGMNINGFVHVGRNYGNAFWYGPYDGMFFGDGDGVYFSSL AKSLDVVGHELSHGVTNKESNLKYENESGALNESFSDIMGVAVEGKNFVLGEDCWVAGGVMRDMEDPSRGGQPAHMKDYK YKPMNDDNGGVHTNSGIINHAAYLVADGIEKTGAKNSKDIMGKIFYTANCYKWDETTNFAKCRNDVVQVTKELYGENSKY VKIVEKAFDQVGITATPQLPL
Sequences:
>Translated_581_residues MKSKKLLATVLSAVITFSTVSAVYAAPVGKESKVEPKTTTITWEKNEQNTKKAATDITEKKFNNSEEITKFFEKNISKFG VQKGSLKNTKTVKDEKGKTNYHMIYEVEGIPVYYGRIVFTTEKDSSMDSINGRIDTVFENGNWKNRIKLSKDNAIAKAKN NIKDEKATSKKTDLYLYNFEGKPYVVYLVDLITDNGSWTIFVNAEDGSIVNKFNNTPTLIDTKGQKLPNAKKIKDEAKKA SNENSVIDVQGQSVKGVGRTSLDGLVNIDLTYGNGRYYLKDSNKNIYLYDLKNQVDEYDLYNYLSRPNYKQILMSKSELI SNYNNNFIADNQVNSVDAYVNTNKTYDYYKNKLNRNSIDNKGMNINGFVHVGRNYGNAFWYGPYDGMFFGDGDGVYFSSL AKSLDVVGHELSHGVTNKESNLKYENESGALNESFSDIMGVAVEGKNFVLGEDCWVAGGVMRDMEDPSRGGQPAHMKDYK YKPMNDDNGGVHTNSGIINHAAYLVADGIEKTGAKNSKDIMGKIFYTANCYKWDETTNFAKCRNDVVQVTKELYGENSKY VKIVEKAFDQVGITATPQLPL >Mature_581_residues MKSKKLLATVLSAVITFSTVSAVYAAPVGKESKVEPKTTTITWEKNEQNTKKAATDITEKKFNNSEEITKFFEKNISKFG VQKGSLKNTKTVKDEKGKTNYHMIYEVEGIPVYYGRIVFTTEKDSSMDSINGRIDTVFENGNWKNRIKLSKDNAIAKAKN NIKDEKATSKKTDLYLYNFEGKPYVVYLVDLITDNGSWTIFVNAEDGSIVNKFNNTPTLIDTKGQKLPNAKKIKDEAKKA SNENSVIDVQGQSVKGVGRTSLDGLVNIDLTYGNGRYYLKDSNKNIYLYDLKNQVDEYDLYNYLSRPNYKQILMSKSELI SNYNNNFIADNQVNSVDAYVNTNKTYDYYKNKLNRNSIDNKGMNINGFVHVGRNYGNAFWYGPYDGMFFGDGDGVYFSSL AKSLDVVGHELSHGVTNKESNLKYENESGALNESFSDIMGVAVEGKNFVLGEDCWVAGGVMRDMEDPSRGGQPAHMKDYK YKPMNDDNGGVHTNSGIINHAAYLVADGIEKTGAKNSKDIMGKIFYTANCYKWDETTNFAKCRNDVVQVTKELYGENSKY VKIVEKAFDQVGITATPQLPL
Specific function: Extracellular zinc metalloprotease [H]
COG id: COG3227
COG function: function code E; Zinc metalloprotease (elastase)
Gene ontology:
Cell location: Secreted [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the peptidase M4 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005075 - InterPro: IPR013856 - InterPro: IPR001570 - InterPro: IPR011096 [H]
Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C [H]
EC number: =3.4.24.28 [H]
Molecular weight: Translated: 65220; Mature: 65220
Theoretical pI: Translated: 8.67; Mature: 8.67
Prosite motif: PS00142 ZINC_PROTEASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKSKKLLATVLSAVITFSTVSAVYAAPVGKESKVEPKTTTITWEKNEQNTKKAATDITEK CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH KFNNSEEITKFFEKNISKFGVQKGSLKNTKTVKDEKGKTNYHMIYEVEGIPVYYGRIVFT HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEEEE TEKDSSMDSINGRIDTVFENGNWKNRIKLSKDNAIAKAKNNIKDEKATSKKTDLYLYNFE ECCCCCHHHCCCEEEEEEECCCCCCEEEECCCCCHHHHHCCCCHHHCCCCCCEEEEEEEC GKPYVVYLVDLITDNGSWTIFVNAEDGSIVNKFNNTPTLIDTKGQKLPNAKKIKDEAKKA CCEEEEEEEEEEECCCCEEEEEECCCCCEEECCCCCCCEEECCCCCCCCHHHHHHHHHHC SNENSVIDVQGQSVKGVGRTSLDGLVNIDLTYGNGRYYLKDSNKNIYLYDLKNQVDEYDL CCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCEEEEEECCCCCCHHHH YNYLSRPNYKQILMSKSELISNYNNNFIADNQVNSVDAYVNTNKTYDYYKNKLNRNSIDN HHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCHHHHHHCCCCCCCCC KGMNINGFVHVGRNYGNAFWYGPYDGMFFGDGDGVYFSSLAKSLDVVGHELSHGVTNKES CCCCEEEEEEECCCCCCEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC NLKYENESGALNESFSDIMGVAVEGKNFVLGEDCWVAGGVMRDMEDPSRGGQPAHMKDYK CCEECCCCCCCCCCHHHHHEEEECCCCEEECCCCEEECCHHHCCCCCCCCCCCCCCCCCC YKPMNDDNGGVHTNSGIINHAAYLVADGIEKTGAKNSKDIMGKIFYTANCYKWDETTNFA CCCCCCCCCCEECCCCHHHHHHHHHCCCHHHCCCCCCHHHEEEEEEEEEEEECCCCCCHH KCRNDVVQVTKELYGENSKYVKIVEKAFDQVGITATPQLPL HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCCCCC >Mature Secondary Structure MKSKKLLATVLSAVITFSTVSAVYAAPVGKESKVEPKTTTITWEKNEQNTKKAATDITEK CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH KFNNSEEITKFFEKNISKFGVQKGSLKNTKTVKDEKGKTNYHMIYEVEGIPVYYGRIVFT HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEEEE TEKDSSMDSINGRIDTVFENGNWKNRIKLSKDNAIAKAKNNIKDEKATSKKTDLYLYNFE ECCCCCHHHCCCEEEEEEECCCCCCEEEECCCCCHHHHHCCCCHHHCCCCCCEEEEEEEC GKPYVVYLVDLITDNGSWTIFVNAEDGSIVNKFNNTPTLIDTKGQKLPNAKKIKDEAKKA CCEEEEEEEEEEECCCCEEEEEECCCCCEEECCCCCCCEEECCCCCCCCHHHHHHHHHHC SNENSVIDVQGQSVKGVGRTSLDGLVNIDLTYGNGRYYLKDSNKNIYLYDLKNQVDEYDL CCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCEEEEEECCCCCCHHHH YNYLSRPNYKQILMSKSELISNYNNNFIADNQVNSVDAYVNTNKTYDYYKNKLNRNSIDN HHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCHHHHHHCCCCCCCCC KGMNINGFVHVGRNYGNAFWYGPYDGMFFGDGDGVYFSSLAKSLDVVGHELSHGVTNKES CCCCEEEEEEECCCCCCEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC NLKYENESGALNESFSDIMGVAVEGKNFVLGEDCWVAGGVMRDMEDPSRGGQPAHMKDYK CCEECCCCCCCCCCHHHHHEEEECCCCEEECCCCEEECCHHHCCCCCCCCCCCCCCCCCC YKPMNDDNGGVHTNSGIINHAAYLVADGIEKTGAKNSKDIMGKIFYTANCYKWDETTNFA CCCCCCCCCCEECCCCHHHHHHHHHCCCHHHCCCCCCHHHEEEEEEEEEEEECCCCCCHH KCRNDVVQVTKELYGENSKYVKIVEKAFDQVGITATPQLPL HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2203733; 3149972 [H]