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The map label for this gene is yflA [H]

Identifier: 226948376

GI number: 226948376

Start: 1314066

End: 1315442

Strand: Reverse

Name: yflA [H]

Synonym: CLM_1259

Alternate gene names: 226948376

Gene position: 1315442-1314066 (Counterclockwise)

Preceding gene: 226948391

Following gene: 226948375

Centisome position: 31.66

GC content: 34.71

Gene sequence:

>1377_bases
ATGGAGGCCTTTATGAATTTTATTAGCAAAATCTCCAACTGGATATGGGGTCCACCTATGTTAATATTATTAGTTGGTGG
TGGAATTATTTTAACTTTTACATTGAATTTTTTCCAAATTAGATATTTTCCTTATATAATGAAAGAAACTTTTGGTAAAA
TGTTCAGTAAACCTGCTGAAGGAGAAGGTACAATTACGCCATTTCAAGCCGCTTGTTCAGCATTAGCCTCTACTGTTGGT
GCTGCCAATATCATAGGAGTTCCTGTGGCAATAGCTTTTGGGGGTCCTGGTGCTGTTTTCTGGATGTGGGTAGTTGCAGT
TTTAGGACAAGCTACTAAATTCTCCGAAATAATTCTTGGTATAAAATACAGAGAAAAGAATGAAGAAGGAAACTATGTAG
GAGGACCTGTTTACTACTTAAAAAAAGGTTTGAAATCACCGTTCCTTGCTACAATGTGTTCTTTCTGCTTTATGATTGAA
ATTATACCATCTATTTCTACACAATCATTATCAGTTTGTCAAACTGCCGAAACTATAGGATTACCAAAGATAGTTACTGC
TATAATTGTAACCGTTTTAGTTGCTCTAGTTGTTTATGGTGGTATTCAAAGAATAGGGCAAGTTACTGAAAAGATGGTTC
CTATAATGGCTTTAATATTCATTATTTGTTCAGTAATAATAATATTATTCAATATCACAAAATTACCAAATGCTTTTGCA
TTGATATTTAAAGGTGCTTTTACACCTCAAGCTGCTGTTGGTGGATTTGGTGGAGCTACACTTGCTCAAGCATTAAGATG
GGGTACTGCTAGAGGTACTTACTCAAATGAAGCAGGTATGGGAAGCGCTCCTATAGCTCACTCTGCTGCAGTTACAGATC
ATCCTGTTCGCCAAGCTTTCTGGGGTATATTTGAAATAATGGTAGATACAATAATAATTTGTACTTTAACAGCTCTTGTT
GTTATAACTACAGGAATGTGGAATACTGTTCCAGGTTCTCAAGCAGCTTCTATGCCTTCAATGGCTTTCCAAAATGTGTT
TGGTAAAGCTTTGGGAGGTGCTATAGTATCAATCAGCTTATTATTATTTGTTCTATCAACTATTATAGTTATAGTTTACT
ATTGTGAAAAACAAGCTGAAGCTTTATTTGGACTCTCATTCTCAAGAATTATAAGAGTTGTTTGTCTAGCAGCTATAATA
TATGGCGCCGTTGGTAAGTTAGAATTCTTATTCGCATTACTTGATATTCTTTTAGCCCTAGTTGTTATACCAAATATGAT
TGGTTTAATAGCTATGAGAAATGAAATAAAAGAATTGAAAGAAGAATTCTTCTCAGACCCAAAATATTACCCAGGGGCAA
AAGCTTCAAAATCTTAG

Upstream 100 bases:

>100_bases
ATAATATTATATATTTATTTTTATAGGTTGAAAGTTTTGACAATAAATGATACAATTTTAACATAGATCTTATTTTTATT
AATATATTAGGAGGGGTATT

Downstream 100 bases:

>100_bases
AGTAAAAATTTAAATATCTAATCTTTAATAATAGACCCCCTTATGAATTTACATAAGGGGGTCATCAATACAATCATAAA
AAACTCCCTCTGGGGAGTTT

Product: sodium:alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MEAFMNFISKISNWIWGPPMLILLVGGGIILTFTLNFFQIRYFPYIMKETFGKMFSKPAEGEGTITPFQAACSALASTVG
AANIIGVPVAIAFGGPGAVFWMWVVAVLGQATKFSEIILGIKYREKNEEGNYVGGPVYYLKKGLKSPFLATMCSFCFMIE
IIPSISTQSLSVCQTAETIGLPKIVTAIIVTVLVALVVYGGIQRIGQVTEKMVPIMALIFIICSVIIILFNITKLPNAFA
LIFKGAFTPQAAVGGFGGATLAQALRWGTARGTYSNEAGMGSAPIAHSAAVTDHPVRQAFWGIFEIMVDTIIICTLTALV
VITTGMWNTVPGSQAASMPSMAFQNVFGKALGGAIVSISLLLFVLSTIIVIVYYCEKQAEALFGLSFSRIIRVVCLAAII
YGAVGKLEFLFALLDILLALVVIPNMIGLIAMRNEIKELKEEFFSDPKYYPGAKASKS

Sequences:

>Translated_458_residues
MEAFMNFISKISNWIWGPPMLILLVGGGIILTFTLNFFQIRYFPYIMKETFGKMFSKPAEGEGTITPFQAACSALASTVG
AANIIGVPVAIAFGGPGAVFWMWVVAVLGQATKFSEIILGIKYREKNEEGNYVGGPVYYLKKGLKSPFLATMCSFCFMIE
IIPSISTQSLSVCQTAETIGLPKIVTAIIVTVLVALVVYGGIQRIGQVTEKMVPIMALIFIICSVIIILFNITKLPNAFA
LIFKGAFTPQAAVGGFGGATLAQALRWGTARGTYSNEAGMGSAPIAHSAAVTDHPVRQAFWGIFEIMVDTIIICTLTALV
VITTGMWNTVPGSQAASMPSMAFQNVFGKALGGAIVSISLLLFVLSTIIVIVYYCEKQAEALFGLSFSRIIRVVCLAAII
YGAVGKLEFLFALLDILLALVVIPNMIGLIAMRNEIKELKEEFFSDPKYYPGAKASKS
>Mature_458_residues
MEAFMNFISKISNWIWGPPMLILLVGGGIILTFTLNFFQIRYFPYIMKETFGKMFSKPAEGEGTITPFQAACSALASTVG
AANIIGVPVAIAFGGPGAVFWMWVVAVLGQATKFSEIILGIKYREKNEEGNYVGGPVYYLKKGLKSPFLATMCSFCFMIE
IIPSISTQSLSVCQTAETIGLPKIVTAIIVTVLVALVVYGGIQRIGQVTEKMVPIMALIFIICSVIIILFNITKLPNAFA
LIFKGAFTPQAAVGGFGGATLAQALRWGTARGTYSNEAGMGSAPIAHSAAVTDHPVRQAFWGIFEIMVDTIIICTLTALV
VITTGMWNTVPGSQAASMPSMAFQNVFGKALGGAIVSISLLLFVLSTIIVIVYYCEKQAEALFGLSFSRIIRVVCLAAII
YGAVGKLEFLFALLDILLALVVIPNMIGLIAMRNEIKELKEEFFSDPKYYPGAKASKS

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=466, Percent_Identity=31.9742489270386, Blast_Score=218, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49317; Mature: 49317

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAFMNFISKISNWIWGPPMLILLVGGGIILTFTLNFFQIRYFPYIMKETFGKMFSKPAE
CHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GEGTITPFQAACSALASTVGAANIIGVPVAIAFGGPGAVFWMWVVAVLGQATKFSEIILG
CCCCCCHHHHHHHHHHHHHCHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHH
IKYREKNEEGNYVGGPVYYLKKGLKSPFLATMCSFCFMIEIIPSISTQSLSVCQTAETIG
HHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
LPKIVTAIIVTVLVALVVYGGIQRIGQVTEKMVPIMALIFIICSVIIILFNITKLPNAFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LIFKGAFTPQAAVGGFGGATLAQALRWGTARGTYSNEAGMGSAPIAHSAAVTDHPVRQAF
HHHHCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
WGIFEIMVDTIIICTLTALVVITTGMWNTVPGSQAASMPSMAFQNVFGKALGGAIVSISL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
LLFVLSTIIVIVYYCEKQAEALFGLSFSRIIRVVCLAAIIYGAVGKLEFLFALLDILLAL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVIPNMIGLIAMRNEIKELKEEFFSDPKYYPGAKASKS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEAFMNFISKISNWIWGPPMLILLVGGGIILTFTLNFFQIRYFPYIMKETFGKMFSKPAE
CHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GEGTITPFQAACSALASTVGAANIIGVPVAIAFGGPGAVFWMWVVAVLGQATKFSEIILG
CCCCCCHHHHHHHHHHHHHCHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHH
IKYREKNEEGNYVGGPVYYLKKGLKSPFLATMCSFCFMIEIIPSISTQSLSVCQTAETIG
HHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
LPKIVTAIIVTVLVALVVYGGIQRIGQVTEKMVPIMALIFIICSVIIILFNITKLPNAFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LIFKGAFTPQAAVGGFGGATLAQALRWGTARGTYSNEAGMGSAPIAHSAAVTDHPVRQAF
HHHHCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
WGIFEIMVDTIIICTLTALVVITTGMWNTVPGSQAASMPSMAFQNVFGKALGGAIVSISL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
LLFVLSTIIVIVYYCEKQAEALFGLSFSRIIRVVCLAAIIYGAVGKLEFLFALLDILLAL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVIPNMIGLIAMRNEIKELKEEFFSDPKYYPGAKASKS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]