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The map label for this gene is thyA [H]

Identifier: 226948057

GI number: 226948057

Start: 976300

End: 977274

Strand: Direct

Name: thyA [H]

Synonym: CLM_0918

Alternate gene names: 226948057

Gene position: 976300-977274 (Clockwise)

Preceding gene: 226948056

Following gene: 226948058

Centisome position: 23.5

GC content: 26.56

Gene sequence:

>975_bases
ATGAAGTTTAAAAATTTTCATGAAGCCTATCTAAGGAATTTAAATGATGTATATTATACCCCTGAGTTTATAAATCAACC
AAGAGGAAATGTAAGTAAAGAAAGATTAAATTATTTTATGATACTAGAAAATCCACGAGAAAGAATTTGTTATACAAAAT
CGAGAAAAACTAATATTGTTTTTAATTTTGCAGAAGCATTATGGTATTTATCAGGATCTAATGATTTAGACTATATTTCT
TATTATGCAAGCAATATGAAAAAATATTCTATGGATGGTAAAACTCTTACAGGAACTGCATATGGTCCAAAGATATTTTC
CTATGGATATAATAAAATTAATCAGTGGAATCGTATAATTAAACTATTTAAAGAAGATCCAGATACAAAGAGAGGATTTA
TTGAAATTTTCGATGCAAATGAAGATTTATCATTAAAGAATATAGATGTTTCTTGCACTATAGGATTTCAATTCTTTATC
CGAGAACATAAGCTATATATGACAACTTTTATGAGAGCAAATGACGCTTATCGAGGAATTATAAGTGATGTTTTTTCCTT
TACTTTTATTCAAGAATTTTTAGCAGCTGAACTAAACTTAGATATAGGACAATACAGTCATCAGGTAGCAACAACACATA
TATATGAGCCAGATTTTAAATTAGCCGAAAAAGTATTATGCGAAAGCAAAAGTGTTAAAAAAGAGCTTTCTTTTCCTAGA
ATGCCTAAAAAGGATAATTGGGAAGATTTAAAAATAGTTCTTCAATATGAAAAAGAGCTAAGGAAACAGAGCATTCATTT
AACAAAGAAAGATATTGATGAATTAGAGATAGATAAATATTGGAAACAAATTATTTGTTTATTTGTACTTTACCAAGAAA
TATATTATGGAGATAATTTGGATTATGAAATATATGAAAGCTTAGAACCACTTTATCAATATCTGTTTTCAAATAAATGG
AATCAATATTTTTAG

Upstream 100 bases:

>100_bases
ATCAGGGGATTACATACTGTAAGTAAGGTACATTATATAATTTATAATGAGGAAAAAGAGTATCTTCAGAAATTAGATTT
ATATTTAGACGGTGAAAATA

Downstream 100 bases:

>100_bases
GAGGGAATATTTTGGGTACATGCTGGAAGGATGAAGAAGTTTCAAAAAAATTTAATTTATATAATGATATGTTAGAAAAT
ATTTTGGGATTTGATTATAT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKFKNFHEAYLRNLNDVYYTPEFINQPRGNVSKERLNYFMILENPRERICYTKSRKTNIVFNFAEALWYLSGSNDLDYIS
YYASNMKKYSMDGKTLTGTAYGPKIFSYGYNKINQWNRIIKLFKEDPDTKRGFIEIFDANEDLSLKNIDVSCTIGFQFFI
REHKLYMTTFMRANDAYRGIISDVFSFTFIQEFLAAELNLDIGQYSHQVATTHIYEPDFKLAEKVLCESKSVKKELSFPR
MPKKDNWEDLKIVLQYEKELRKQSIHLTKKDIDELEIDKYWKQIICLFVLYQEIYYGDNLDYEIYESLEPLYQYLFSNKW
NQYF

Sequences:

>Translated_324_residues
MKFKNFHEAYLRNLNDVYYTPEFINQPRGNVSKERLNYFMILENPRERICYTKSRKTNIVFNFAEALWYLSGSNDLDYIS
YYASNMKKYSMDGKTLTGTAYGPKIFSYGYNKINQWNRIIKLFKEDPDTKRGFIEIFDANEDLSLKNIDVSCTIGFQFFI
REHKLYMTTFMRANDAYRGIISDVFSFTFIQEFLAAELNLDIGQYSHQVATTHIYEPDFKLAEKVLCESKSVKKELSFPR
MPKKDNWEDLKIVLQYEKELRKQSIHLTKKDIDELEIDKYWKQIICLFVLYQEIYYGDNLDYEIYESLEPLYQYLFSNKW
NQYF
>Mature_324_residues
MKFKNFHEAYLRNLNDVYYTPEFINQPRGNVSKERLNYFMILENPRERICYTKSRKTNIVFNFAEALWYLSGSNDLDYIS
YYASNMKKYSMDGKTLTGTAYGPKIFSYGYNKINQWNRIIKLFKEDPDTKRGFIEIFDANEDLSLKNIDVSCTIGFQFFI
REHKLYMTTFMRANDAYRGIISDVFSFTFIQEFLAAELNLDIGQYSHQVATTHIYEPDFKLAEKVLCESKSVKKELSFPR
MPKKDNWEDLKIVLQYEKELRKQSIHLTKKDIDELEIDKYWKQIICLFVLYQEIYYGDNLDYEIYESLEPLYQYLFSNKW
NQYF

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 38963; Mature: 38963

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKNFHEAYLRNLNDVYYTPEFINQPRGNVSKERLNYFMILENPRERICYTKSRKTNIV
CCCCHHHHHHHHCCCCCEECHHHHCCCCCCCCHHHCCEEEEEECCHHHHEEECCCCCEEE
FNFAEALWYLSGSNDLDYISYYASNMKKYSMDGKTLTGTAYGPKIFSYGYNKINQWNRII
EEEHHHHHEECCCCCHHHHHHHHHCCCEEECCCCEEECCCCCCHHHHHCCHHHHHHHHHH
KLFKEDPDTKRGFIEIFDANEDLSLKNIDVSCTIGFQFFIREHKLYMTTFMRANDAYRGI
HHHHCCCCCCCCEEEEEECCCCCEEEEEEEEEEEHHEEHHHHHHHHHHHHHHCCHHHHHH
ISDVFSFTFIQEFLAAELNLDIGQYSHQVATTHIYEPDFKLAEKVLCESKSVKKELSFPR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCHHHHHHHHHCCHHHHHHCCCCC
MPKKDNWEDLKIVLQYEKELRKQSIHLTKKDIDELEIDKYWKQIICLFVLYQEIYYGDNL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DYEIYESLEPLYQYLFSNKWNQYF
CHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKFKNFHEAYLRNLNDVYYTPEFINQPRGNVSKERLNYFMILENPRERICYTKSRKTNIV
CCCCHHHHHHHHCCCCCEECHHHHCCCCCCCCHHHCCEEEEEECCHHHHEEECCCCCEEE
FNFAEALWYLSGSNDLDYISYYASNMKKYSMDGKTLTGTAYGPKIFSYGYNKINQWNRII
EEEHHHHHEECCCCCHHHHHHHHHCCCEEECCCCEEECCCCCCHHHHHCCHHHHHHHHHH
KLFKEDPDTKRGFIEIFDANEDLSLKNIDVSCTIGFQFFIREHKLYMTTFMRANDAYRGI
HHHHCCCCCCCCEEEEEECCCCCEEEEEEEEEEEHHEEHHHHHHHHHHHHHHCCHHHHHH
ISDVFSFTFIQEFLAAELNLDIGQYSHQVATTHIYEPDFKLAEKVLCESKSVKKELSFPR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCHHHHHHHHHCCHHHHHHCCCCC
MPKKDNWEDLKIVLQYEKELRKQSIHLTKKDIDELEIDKYWKQIICLFVLYQEIYYGDNL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DYEIYESLEPLYQYLFSNKWNQYF
CHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]