| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is helD [H]
Identifier: 226947991
GI number: 226947991
Start: 909118
End: 911376
Strand: Direct
Name: helD [H]
Synonym: CLM_0852
Alternate gene names: 226947991
Gene position: 909118-911376 (Clockwise)
Preceding gene: 226947990
Following gene: 226947994
Centisome position: 21.88
GC content: 33.16
Gene sequence:
>2259_bases ATGAAGGATTTTGAATGGAAGCTTGAAAAGGAATGGCTACAGGAGGTGCTTCAAGAGGTTCGAAAACAGTATAATGAAAA ACATGATCTTAAAGAAAAATTTAAAAAGGACGCTATTGAAACACAACGGGAACTATGGGATGACGTAGGCTCTGTTTCCA TAGCCAATGGATTAGAGCAGGTTGTAGACTTTATAGGATTTATCAATACCATGAAAATACAGAAGAGAAGCCACGAGTTC ACAAAAAAACTTGAGGAAAGATACGAAAGGATGCTCCTATCACCCTACTTTGGAAGGATGGACTTTATTGAAAATGGAGG GGAGAAAGCTGAAAAGTATTATATTGGAATTTCAAATCTTATTAATGATAACTATGATTTTCTCGTATACGACTGGAGAG CACCGGTTTCCAGCATGTTTTATGATTACGAAATTGGAGAAGCCAATTATAAATGTCCTGAAGGAAGGGTAAATGGAAAA CTTACACTGAAAAGACAGTACAAAATTAATAATGGTGAAATTAAGTATATGTTTGACAGCAATCTTAAGATAGACGATGA AGTGCTACAGGATATCTTGGGTAAAAGCACGGATAGTAAGATGAATGCAATAGTAACAACTATTCAAAGAGAGCAAAATA AAGTAATTCGTAATGAGGAGTATAAAAATTTGATTGTGCAGGGTCCTGCTGGAAGTGGGAAAACCTCTGTTGCTCTTCAT AGAATTGCCTATCTATTATATAAGCATAGGGATAAAATAAGCCCTAAAAATATAGTAATATTCTCTCCAAACGATATTTT TAATGATTATATTTCTAATGTATTGCCCCAGCTTGGAGAAGATAATATGTGTCAGACCACATTTAAGGAATACATGCACA AGGCTTTAGGTAATGGGCTTGTAAAGGAAAACTATTCTGAGATGATGGAGTATATTCTTGTCTCTAAGAAGGATGGTGAT TATGAAAAGAGAATAAACAATATAAAATTTAAGTCTTCCATGGAGTTTATAGATATATTAAAAGCATATGTGGGCTACCT AGAAAAAATGGATAGAAATTTTACAGATATTACTTTCAGAGGGGATTTGATAATCTCATCTAAGGATATAGAAGAATTAT TTTTTAAAGATTATGTAAAGCTTCCAATTAAAAGAAGGCTTCAAAAGATAAGAAGAAGAATTTTGTTCCTAATGGAGCCT TATGAAAAACATTGGGTAAAAGAAGAGGCTGAGGAGCTTAAGAACTCAGGTAACTATATAGATAATGTAGAAATTACGGA GCGGAGTATAAATATTGTAAAGGATGAAATAAAGGATATATACCATGAAATTAGTAGAATGACAGACTTTGATTTAGTGG ATGTTTATAAAAAGCTTTTTGAAAACCTGAAGTTTTTCTTAAAAAAATCAAATATCGAATACCATGAGAAAAGTATTGAT GAAATCAAAAACTATACTTTAGAAATCTTAAGAATAGGAAAACTTAACTATGAGGATCAACCACCTCTTTTGTATCTAAA AGGTGCCTTAGGAGACCTGCCAAAAACTTCAGAAATTAAATATGTTATTATTGATGAGGCTCAGGATTATACACCGCTAC AGTATGAAATCTTTTCTCAGCTTTTCAACCATGCTAATATGACTATACTAGGAGATTTACATCAATCCATCAATCCCTTC ATGAAATTGGGGGATTATGGTAATATATCCAATGTATTCCCAAAGGATAATACATGCATAATAAATTTAACCAAGAGTTA CAGATCCACAATGGAAATTACTAGGTTTTCAAGAAGGATACTTAACAAGAAGATTACTGACCAATGGGTAGAAAGAAGTG GAGATAAGCCTTTAGTCCTTGGTTCCCCAAATGAAGAGGCTATAAAGGAAAGACTTCTAAAGGATATAAAAATATATAAG GAGAAAGGCTACAAATCTATTGGCATCATAACAAGGACTATAAAGGAAGCGCAAGAGGTACACAGTTTCTTAAAAGACAA AATTCATGTTAAAGCCATAATGAAGGATGATGATGAATATGTGAGTGATACCTTGGTAATCCCTGCTTATCTTTCTAAAG GATTAGAATTTGATGTGGTTTTAATATATAATGCAGGGGATGAAAATTACAGCTGCCAAGAGGAAAGGCTGCTGCTTTAT ACTGCGTGTACTAGGGCACTTCATATTTTATGCATATACTATTCAGGAAAGGTTACGCCTTTGTTAGTAGGAAGTATTGA ATTTAGCACTTTAAAATAG
Upstream 100 bases:
>100_bases CTAACTGTTAAAATATAATAATCAAATATAGGACTATACTTCATGTAAAAAAGACATTTGTCATTGGGCGAATGTCTTTA CTATGTAAGGAGGGGATATC
Downstream 100 bases:
>100_bases AAGACAATTTTAATTGTTAAAAAGCTGTAGATAAAACTACAGCTTTTTTAGTCTACAGAAGGCTAGTACATAATTTAGAT CAAAAAGTTCATATCTGACT
Product: hypothetical protein
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 752; Mature: 752
Protein sequence:
>752_residues MKDFEWKLEKEWLQEVLQEVRKQYNEKHDLKEKFKKDAIETQRELWDDVGSVSIANGLEQVVDFIGFINTMKIQKRSHEF TKKLEERYERMLLSPYFGRMDFIENGGEKAEKYYIGISNLINDNYDFLVYDWRAPVSSMFYDYEIGEANYKCPEGRVNGK LTLKRQYKINNGEIKYMFDSNLKIDDEVLQDILGKSTDSKMNAIVTTIQREQNKVIRNEEYKNLIVQGPAGSGKTSVALH RIAYLLYKHRDKISPKNIVIFSPNDIFNDYISNVLPQLGEDNMCQTTFKEYMHKALGNGLVKENYSEMMEYILVSKKDGD YEKRINNIKFKSSMEFIDILKAYVGYLEKMDRNFTDITFRGDLIISSKDIEELFFKDYVKLPIKRRLQKIRRRILFLMEP YEKHWVKEEAEELKNSGNYIDNVEITERSINIVKDEIKDIYHEISRMTDFDLVDVYKKLFENLKFFLKKSNIEYHEKSID EIKNYTLEILRIGKLNYEDQPPLLYLKGALGDLPKTSEIKYVIIDEAQDYTPLQYEIFSQLFNHANMTILGDLHQSINPF MKLGDYGNISNVFPKDNTCIINLTKSYRSTMEITRFSRRILNKKITDQWVERSGDKPLVLGSPNEEAIKERLLKDIKIYK EKGYKSIGIITRTIKEAQEVHSFLKDKIHVKAIMKDDDEYVSDTLVIPAYLSKGLEFDVVLIYNAGDENYSCQEERLLLY TACTRALHILCIYYSGKVTPLLVGSIEFSTLK
Sequences:
>Translated_752_residues MKDFEWKLEKEWLQEVLQEVRKQYNEKHDLKEKFKKDAIETQRELWDDVGSVSIANGLEQVVDFIGFINTMKIQKRSHEF TKKLEERYERMLLSPYFGRMDFIENGGEKAEKYYIGISNLINDNYDFLVYDWRAPVSSMFYDYEIGEANYKCPEGRVNGK LTLKRQYKINNGEIKYMFDSNLKIDDEVLQDILGKSTDSKMNAIVTTIQREQNKVIRNEEYKNLIVQGPAGSGKTSVALH RIAYLLYKHRDKISPKNIVIFSPNDIFNDYISNVLPQLGEDNMCQTTFKEYMHKALGNGLVKENYSEMMEYILVSKKDGD YEKRINNIKFKSSMEFIDILKAYVGYLEKMDRNFTDITFRGDLIISSKDIEELFFKDYVKLPIKRRLQKIRRRILFLMEP YEKHWVKEEAEELKNSGNYIDNVEITERSINIVKDEIKDIYHEISRMTDFDLVDVYKKLFENLKFFLKKSNIEYHEKSID EIKNYTLEILRIGKLNYEDQPPLLYLKGALGDLPKTSEIKYVIIDEAQDYTPLQYEIFSQLFNHANMTILGDLHQSINPF MKLGDYGNISNVFPKDNTCIINLTKSYRSTMEITRFSRRILNKKITDQWVERSGDKPLVLGSPNEEAIKERLLKDIKIYK EKGYKSIGIITRTIKEAQEVHSFLKDKIHVKAIMKDDDEYVSDTLVIPAYLSKGLEFDVVLIYNAGDENYSCQEERLLLY TACTRALHILCIYYSGKVTPLLVGSIEFSTLK >Mature_752_residues MKDFEWKLEKEWLQEVLQEVRKQYNEKHDLKEKFKKDAIETQRELWDDVGSVSIANGLEQVVDFIGFINTMKIQKRSHEF TKKLEERYERMLLSPYFGRMDFIENGGEKAEKYYIGISNLINDNYDFLVYDWRAPVSSMFYDYEIGEANYKCPEGRVNGK LTLKRQYKINNGEIKYMFDSNLKIDDEVLQDILGKSTDSKMNAIVTTIQREQNKVIRNEEYKNLIVQGPAGSGKTSVALH RIAYLLYKHRDKISPKNIVIFSPNDIFNDYISNVLPQLGEDNMCQTTFKEYMHKALGNGLVKENYSEMMEYILVSKKDGD YEKRINNIKFKSSMEFIDILKAYVGYLEKMDRNFTDITFRGDLIISSKDIEELFFKDYVKLPIKRRLQKIRRRILFLMEP YEKHWVKEEAEELKNSGNYIDNVEITERSINIVKDEIKDIYHEISRMTDFDLVDVYKKLFENLKFFLKKSNIEYHEKSID EIKNYTLEILRIGKLNYEDQPPLLYLKGALGDLPKTSEIKYVIIDEAQDYTPLQYEIFSQLFNHANMTILGDLHQSINPF MKLGDYGNISNVFPKDNTCIINLTKSYRSTMEITRFSRRILNKKITDQWVERSGDKPLVLGSPNEEAIKERLLKDIKIYK EKGYKSIGIITRTIKEAQEVHSFLKDKIHVKAIMKDDDEYVSDTLVIPAYLSKGLEFDVVLIYNAGDENYSCQEERLLLY TACTRALHILCIYYSGKVTPLLVGSIEFSTLK
Specific function: Catalyzes the unwinding of duplex DNA in the 3' to 5' direction; this reaction is dependent on the hydrolysis of ATP [H]
COG id: COG3973
COG function: function code R; Superfamily I DNA and RNA helicases
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000212 - InterPro: IPR014016 [H]
Pfam domain/function: PF00580 UvrD-helicase [H]
EC number: =3.6.4.12 [H]
Molecular weight: Translated: 88413; Mature: 88413
Theoretical pI: Translated: 6.48; Mature: 6.48
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKDFEWKLEKEWLQEVLQEVRKQYNEKHDLKEKFKKDAIETQRELWDDVGSVSIANGLEQ CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH VVDFIGFINTMKIQKRSHEFTKKLEERYERMLLSPYFGRMDFIENGGEKAEKYYIGISNL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHCCCCHHHHEEEEHHHH INDNYDFLVYDWRAPVSSMFYDYEIGEANYKCPEGRVNGKLTLKRQYKINNGEIKYMFDS HCCCCCEEEEECCCCHHHHHEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEECC NLKIDDEVLQDILGKSTDSKMNAIVTTIQREQNKVIRNEEYKNLIVQGPAGSGKTSVALH CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH RIAYLLYKHRDKISPKNIVIFSPNDIFNDYISNVLPQLGEDNMCQTTFKEYMHKALGNGL HHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCH VKENYSEMMEYILVSKKDGDYEKRINNIKFKSSMEFIDILKAYVGYLEKMDRNFTDITFR HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE GDLIISSKDIEELFFKDYVKLPIKRRLQKIRRRILFLMEPYEKHWVKEEAEELKNSGNYI CCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEE DNVEITERSINIVKDEIKDIYHEISRMTDFDLVDVYKKLFENLKFFLKKSNIEYHEKSID CCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH EIKNYTLEILRIGKLNYEDQPPLLYLKGALGDLPKTSEIKYVIIDEAQDYTPLQYEIFSQ HHHHHHHHHHEECCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH LFNHANMTILGDLHQSINPFMKLGDYGNISNVFPKDNTCIINLTKSYRSTMEITRFSRRI HHCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH LNKKITDQWVERSGDKPLVLGSPNEEAIKERLLKDIKIYKEKGYKSIGIITRTIKEAQEV HHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH HSFLKDKIHVKAIMKDDDEYVSDTLVIPAYLSKGLEFDVVLIYNAGDENYSCQEERLLLY HHHHHHHHEEEEEECCCHHHHHHHEEEHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHH TACTRALHILCIYYSGKVTPLLVGSIEFSTLK HHHHHHHHEEEEEECCCCCEEEEECEECCCCC >Mature Secondary Structure MKDFEWKLEKEWLQEVLQEVRKQYNEKHDLKEKFKKDAIETQRELWDDVGSVSIANGLEQ CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH VVDFIGFINTMKIQKRSHEFTKKLEERYERMLLSPYFGRMDFIENGGEKAEKYYIGISNL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHCCCCHHHHEEEEHHHH INDNYDFLVYDWRAPVSSMFYDYEIGEANYKCPEGRVNGKLTLKRQYKINNGEIKYMFDS HCCCCCEEEEECCCCHHHHHEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEECC NLKIDDEVLQDILGKSTDSKMNAIVTTIQREQNKVIRNEEYKNLIVQGPAGSGKTSVALH CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH RIAYLLYKHRDKISPKNIVIFSPNDIFNDYISNVLPQLGEDNMCQTTFKEYMHKALGNGL HHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCH VKENYSEMMEYILVSKKDGDYEKRINNIKFKSSMEFIDILKAYVGYLEKMDRNFTDITFR HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE GDLIISSKDIEELFFKDYVKLPIKRRLQKIRRRILFLMEPYEKHWVKEEAEELKNSGNYI CCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEE DNVEITERSINIVKDEIKDIYHEISRMTDFDLVDVYKKLFENLKFFLKKSNIEYHEKSID CCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH EIKNYTLEILRIGKLNYEDQPPLLYLKGALGDLPKTSEIKYVIIDEAQDYTPLQYEIFSQ HHHHHHHHHHEECCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH LFNHANMTILGDLHQSINPFMKLGDYGNISNVFPKDNTCIINLTKSYRSTMEITRFSRRI HHCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH LNKKITDQWVERSGDKPLVLGSPNEEAIKERLLKDIKIYKEKGYKSIGIITRTIKEAQEV HHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH HSFLKDKIHVKAIMKDDDEYVSDTLVIPAYLSKGLEFDVVLIYNAGDENYSCQEERLLLY HHHHHHHHEEEEEECCCHHHHHHHEEEHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHH TACTRALHILCIYYSGKVTPLLVGSIEFSTLK HHHHHHHHEEEEEECCCCCEEEEECEECCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]