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The map label for this gene is hsdM [H]

Identifier: 226947934

GI number: 226947934

Start: 851251

End: 852735

Strand: Direct

Name: hsdM [H]

Synonym: CLM_0792

Alternate gene names: 226947934

Gene position: 851251-852735 (Clockwise)

Preceding gene: 226947933

Following gene: 226947935

Centisome position: 20.49

GC content: 30.77

Gene sequence:

>1485_bases
ATGATTACAGGTGAGTTAAAAAGTAAAATAGATAGAATTTGGGAAACCTTTTGGACTGGTGGTATCACTAATCCATTAGA
GGTTATAGAACAATTTACATATTTTTTATTTATAAAAGATTTAGATGATAACGAAATATTAGCTGAAAGTGATGCTGAAT
TACTTGGCATACCCTTTGAGGGAATGTTTCCAAGTGATAAGCAGTATTTAAGATGGAGTAAATTTAAAAATGAAGAAGCA
GGGGAAATGTATAGGATAGTATCACAAGAAGTTTTCCCTTTTATAAAAGACATTCATGGAGATAAACAATCTGCATACTC
AAAGTATATGTCAGATGCTATGTTTAAAATACCAACTCCACTTATGTTATCTAAAATAGTTGATGCAATAGATAATCTTG
AAATACAGGATAAAGATACTAAGGGAGATCTGTATGAATATCTTTTATCTAAGGTAGCTACAGCGGGTACAAATGGACAA
TTTAGAACTCCAAGACACATAATAAAAATGATGGCAGAATTAATGAAACCAACACCAGAGGATATTATAGTAGACCCAGC
TATGGGTACAGCAGGTTTCCTAGTAGGAGCAGAAGAGTATCTAAGAGAAAAGCATAATGATTTATTCCTAGTTCAAGGAT
TAAAAGACCATTTTAACAACAAGATGTTTAATGGGTTTGATATGGACAGAACTATGCTTAGAATTGGTGCTATGAATATG
ATGCTTCACGGGGTGGACAATCCTAATATTGAGTACAAGGATTCACTTTCGGAAACTAATAAGGATAGTGAAAAATATAC
TCTAGTACTTGCAAATCCTCCATTTAAAGGTAGTTTAGACTATGAAGCTGTTTCAGCAGATATTTTAAAAGTTAGTAAAA
CAAAGAAAACAGAATTATTATTTTTAGCATTATTTTTAAGAATATTAAAAAAAGGTGGAAGATGTGCATCCATTGTTCCA
GATGGGGTTTTATTTGGTTCAACTAAAGGGCATAAGGATATAAGACGTGAAATAGTTGATAACAATAAACTTGAAGCTAT
AATAAGTATGCCAAGTGGAGTATTTAAACCATATGCAGGAGTATCTACAGCAATTATCATATTTACTAAAACTGGTACTG
GCGGAACAGATAAAGTTTGGTTTTATGATATGAAAGCAGATGGATATTCCTTAGATGATAAGAGAAATCCTATTGAAGAT
AATGATATACCAGATATGATAGAAAGATTTAATAATTTGGATAAAGAAGAAGATAGAAAAAGAACAGAACAATCCTTCTT
TGTACCAGTAGATGAAATAAGAGAAAATAATTATGACTTATCCATTAATAAATACAAAGAAATAGAGTATGAAGAAGTAG
TATATGATGAACCTAAAGTTATTTTAGAGAGAGTTAAGAAATTGGAAGAAGAGATAACTGAGGGAATAGATGAATTAGAG
AAAATGATAGAAGTGGGTGAAGAAAATGGATATAAAAAAGATTAA

Upstream 100 bases:

>100_bases
AAGCTAATGCAATTATTTTTTACATATAAATAAGAAGAAATTATTAAAATAAAAAGCTAAATTATATTATAATAATACAA
CTAGTGATGGAGGAAACATA

Downstream 100 bases:

>100_bases
ATGTTCAGAAATAATTGATGTTAGAGATGGAACGCATGATTCACCTAGATATCAATCGAAAGGATATCCATTAGTAACCT
CAAAAAATATTAAGGGCAAT

Product: type I restriction modification system M subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MITGELKSKIDRIWETFWTGGITNPLEVIEQFTYFLFIKDLDDNEILAESDAELLGIPFEGMFPSDKQYLRWSKFKNEEA
GEMYRIVSQEVFPFIKDIHGDKQSAYSKYMSDAMFKIPTPLMLSKIVDAIDNLEIQDKDTKGDLYEYLLSKVATAGTNGQ
FRTPRHIIKMMAELMKPTPEDIIVDPAMGTAGFLVGAEEYLREKHNDLFLVQGLKDHFNNKMFNGFDMDRTMLRIGAMNM
MLHGVDNPNIEYKDSLSETNKDSEKYTLVLANPPFKGSLDYEAVSADILKVSKTKKTELLFLALFLRILKKGGRCASIVP
DGVLFGSTKGHKDIRREIVDNNKLEAIISMPSGVFKPYAGVSTAIIIFTKTGTGGTDKVWFYDMKADGYSLDDKRNPIED
NDIPDMIERFNNLDKEEDRKRTEQSFFVPVDEIRENNYDLSINKYKEIEYEEVVYDEPKVILERVKKLEEEITEGIDELE
KMIEVGEENGYKKD

Sequences:

>Translated_494_residues
MITGELKSKIDRIWETFWTGGITNPLEVIEQFTYFLFIKDLDDNEILAESDAELLGIPFEGMFPSDKQYLRWSKFKNEEA
GEMYRIVSQEVFPFIKDIHGDKQSAYSKYMSDAMFKIPTPLMLSKIVDAIDNLEIQDKDTKGDLYEYLLSKVATAGTNGQ
FRTPRHIIKMMAELMKPTPEDIIVDPAMGTAGFLVGAEEYLREKHNDLFLVQGLKDHFNNKMFNGFDMDRTMLRIGAMNM
MLHGVDNPNIEYKDSLSETNKDSEKYTLVLANPPFKGSLDYEAVSADILKVSKTKKTELLFLALFLRILKKGGRCASIVP
DGVLFGSTKGHKDIRREIVDNNKLEAIISMPSGVFKPYAGVSTAIIIFTKTGTGGTDKVWFYDMKADGYSLDDKRNPIED
NDIPDMIERFNNLDKEEDRKRTEQSFFVPVDEIRENNYDLSINKYKEIEYEEVVYDEPKVILERVKKLEEEITEGIDELE
KMIEVGEENGYKKD
>Mature_494_residues
MITGELKSKIDRIWETFWTGGITNPLEVIEQFTYFLFIKDLDDNEILAESDAELLGIPFEGMFPSDKQYLRWSKFKNEEA
GEMYRIVSQEVFPFIKDIHGDKQSAYSKYMSDAMFKIPTPLMLSKIVDAIDNLEIQDKDTKGDLYEYLLSKVATAGTNGQ
FRTPRHIIKMMAELMKPTPEDIIVDPAMGTAGFLVGAEEYLREKHNDLFLVQGLKDHFNNKMFNGFDMDRTMLRIGAMNM
MLHGVDNPNIEYKDSLSETNKDSEKYTLVLANPPFKGSLDYEAVSADILKVSKTKKTELLFLALFLRILKKGGRCASIVP
DGVLFGSTKGHKDIRREIVDNNKLEAIISMPSGVFKPYAGVSTAIIIFTKTGTGGTDKVWFYDMKADGYSLDDKRNPIED
NDIPDMIERFNNLDKEEDRKRTEQSFFVPVDEIRENNYDLSINKYKEIEYEEVVYDEPKVILERVKKLEEEITEGIDELE
KMIEVGEENGYKKD

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=456, Percent_Identity=29.3859649122807, Blast_Score=158, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 56692; Mature: 56692

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGELKSKIDRIWETFWTGGITNPLEVIEQFTYFLFIKDLDDNEILAESDAELLGIPFE
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEECCCCCEEECCCCCEEECCCC
GMFPSDKQYLRWSKFKNEEAGEMYRIVSQEVFPFIKDIHGDKQSAYSKYMSDAMFKIPTP
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCH
LMLSKIVDAIDNLEIQDKDTKGDLYEYLLSKVATAGTNGQFRTPRHIIKMMAELMKPTPE
HHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
DIIVDPAMGTAGFLVGAEEYLREKHNDLFLVQGLKDHFNNKMFNGFDMDRTMLRIGAMNM
CEEECCCCCCHHHHCCHHHHHHHHCCCEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHH
MLHGVDNPNIEYKDSLSETNKDSEKYTLVLANPPFKGSLDYEAVSADILKVSKTKKTELL
HEECCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHH
FLALFLRILKKGGRCASIVPDGVLFGSTKGHKDIRREIVDNNKLEAIISMPSGVFKPYAG
HHHHHHHHHHCCCCEEEECCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
VSTAIIIFTKTGTGGTDKVWFYDMKADGYSLDDKRNPIEDNDIPDMIERFNNLDKEEDRK
CCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
RTEQSFFVPVDEIRENNYDLSINKYKEIEYEEVVYDEPKVILERVKKLEEEITEGIDELE
HHHHHCCCCHHHHHCCCCCEEECHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KMIEVGEENGYKKD
HHHHHHHCCCCCCC
>Mature Secondary Structure
MITGELKSKIDRIWETFWTGGITNPLEVIEQFTYFLFIKDLDDNEILAESDAELLGIPFE
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEECCCCCEEECCCCCEEECCCC
GMFPSDKQYLRWSKFKNEEAGEMYRIVSQEVFPFIKDIHGDKQSAYSKYMSDAMFKIPTP
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCH
LMLSKIVDAIDNLEIQDKDTKGDLYEYLLSKVATAGTNGQFRTPRHIIKMMAELMKPTPE
HHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
DIIVDPAMGTAGFLVGAEEYLREKHNDLFLVQGLKDHFNNKMFNGFDMDRTMLRIGAMNM
CEEECCCCCCHHHHCCHHHHHHHHCCCEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHH
MLHGVDNPNIEYKDSLSETNKDSEKYTLVLANPPFKGSLDYEAVSADILKVSKTKKTELL
HEECCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHH
FLALFLRILKKGGRCASIVPDGVLFGSTKGHKDIRREIVDNNKLEAIISMPSGVFKPYAG
HHHHHHHHHHCCCCEEEECCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
VSTAIIIFTKTGTGGTDKVWFYDMKADGYSLDDKRNPIEDNDIPDMIERFNNLDKEEDRK
CCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
RTEQSFFVPVDEIRENNYDLSINKYKEIEYEEVVYDEPKVILERVKKLEEEITEGIDELE
HHHHHCCCCHHHHHCCCCCEEECHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KMIEVGEENGYKKD
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]