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The map label for this gene is flp [H]

Identifier: 226947913

GI number: 226947913

Start: 830966

End: 831676

Strand: Direct

Name: flp [H]

Synonym: CLM_0771

Alternate gene names: 226947913

Gene position: 830966-831676 (Clockwise)

Preceding gene: 226947912

Following gene: 226947914

Centisome position: 20.0

GC content: 27.29

Gene sequence:

>711_bases
ATGAAAGAGTGTACTTGTAGAAGATGTCATCACAATATTTGTGCAAGGAAAGTTCCTATATTTTCTTCTTTATCGGAAGA
AGAATTAATAAAAATTGTGAACATGACAGGGCATGAGGATTTTAAAAAAGGAGAAGCTATTTGCAATGAAGGAGATAAAT
CAGAAACTTTAATTATTATAAATGAAGGAAAAGTAAAACTTTCTAAGATTACTAAAGAAGGAAAAGAACAAATAATTCAT
ATACTTTCCAGTGGAGATTTCTTTGGAGAATTAAATCTTTTTAATGATGATAAAAGTTATAATTTCTCCGCTTATGCTAT
ATCCGATGTAAAAATATGTACTCTTACAAAAAATAAGATGGACGAGATATTATTAAAAAATCCAGATATATCTTTAAAGA
TATTAAAAGAAGTTACTAGAAGACTTGCAGAAACGGAAACTTTAGCCCAAAATTTAGCTACCAATGATGCAGATATTAGA
ATAGCTAATATGATATTAGATTTTGGTGAAAAATATGGAGTAAGTAAAGAACAATATATAGAAATAAAGCTGCCTATAAA
TAGGGAAGAGATGGCAAACTATGTAGGGGTTACAAGAGAAACTATAAGTAGAAAATTATCTAAATTCGAGGATTTAGATA
TTATAAAACTTGTTGGTAATAAGGTAATCATAATTAAAGATGAAGATGCATTAAAAGAATTTATAGAATAG

Upstream 100 bases:

>100_bases
TTTAGTTGTAGAGCGCAATATTATATTAATGATATACTAACAGAAACTATAGTTACAAATATAAATTAGTTTTTTATATT
AAATAAAGGGGATGCTTTTT

Downstream 100 bases:

>100_bases
ATTATTTTAGAGAAAAGTGCTTTGCACTTTTTTATTTTTTTGAAAATTTGATTTGAATCATATTTTTAAGTTCCAATAGT
TTATATAATAAAGCCATAAA

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKECTCRRCHHNICARKVPIFSSLSEEELIKIVNMTGHEDFKKGEAICNEGDKSETLIIINEGKVKLSKITKEGKEQIIH
ILSSGDFFGELNLFNDDKSYNFSAYAISDVKICTLTKNKMDEILLKNPDISLKILKEVTRRLAETETLAQNLATNDADIR
IANMILDFGEKYGVSKEQYIEIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE

Sequences:

>Translated_236_residues
MKECTCRRCHHNICARKVPIFSSLSEEELIKIVNMTGHEDFKKGEAICNEGDKSETLIIINEGKVKLSKITKEGKEQIIH
ILSSGDFFGELNLFNDDKSYNFSAYAISDVKICTLTKNKMDEILLKNPDISLKILKEVTRRLAETETLAQNLATNDADIR
IANMILDFGEKYGVSKEQYIEIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE
>Mature_236_residues
MKECTCRRCHHNICARKVPIFSSLSEEELIKIVNMTGHEDFKKGEAICNEGDKSETLIIINEGKVKLSKITKEGKEQIIH
ILSSGDFFGELNLFNDDKSYNFSAYAISDVKICTLTKNKMDEILLKNPDISLKILKEVTRRLAETETLAQNLATNDADIR
IANMILDFGEKYGVSKEQYIEIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE

Specific function: This Protein Complexes With Cyclic AMP And Binds To Specific DNA Sites Near The Promoter To Regulate The Transcription Of Several Catabolite-Sensitive Operons. The Protein Induces A Severe Bend In The DNA. Acts As A Negative Regulator Of Its Own Synthesi

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=185, Percent_Identity=30.2702702702703, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787595, Length=230, Percent_Identity=25.6521739130435, Blast_Score=81, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17137764, Length=98, Percent_Identity=34.6938775510204, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45552207, Length=98, Percent_Identity=34.6938775510204, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45552211, Length=98, Percent_Identity=34.6938775510204, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17137766, Length=98, Percent_Identity=34.6938775510204, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI62471593, Length=98, Percent_Identity=34.6938775510204, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17137770, Length=98, Percent_Identity=34.6938775510204, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI281364342, Length=98, Percent_Identity=34.6938775510204, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45552209, Length=98, Percent_Identity=34.6938775510204, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45552213, Length=98, Percent_Identity=34.6938775510204, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI19549955, Length=98, Percent_Identity=34.6938775510204, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17137768, Length=98, Percent_Identity=34.6938775510204, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26923; Mature: 26923

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKECTCRRCHHNICARKVPIFSSLSEEELIKIVNMTGHEDFKKGEAICNEGDKSETLIII
CCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEE
NEGKVKLSKITKEGKEQIIHILSSGDFFGELNLFNDDKSYNFSAYAISDVKICTLTKNKM
ECCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCEEEEEECCCHH
DEILLKNPDISLKILKEVTRRLAETETLAQNLATNDADIRIANMILDFGEKYGVSKEQYI
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHEE
EIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECHHHHHHHCC
>Mature Secondary Structure
MKECTCRRCHHNICARKVPIFSSLSEEELIKIVNMTGHEDFKKGEAICNEGDKSETLIII
CCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEE
NEGKVKLSKITKEGKEQIIHILSSGDFFGELNLFNDDKSYNFSAYAISDVKICTLTKNKM
ECCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCEEEEEECCCHH
DEILLKNPDISLKILKEVTRRLAETETLAQNLATNDADIRIANMILDFGEKYGVSKEQYI
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHEE
EIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]