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The map label for this gene is yclK [H]

Identifier: 226947860

GI number: 226947860

Start: 769220

End: 770641

Strand: Direct

Name: yclK [H]

Synonym: CLM_0715

Alternate gene names: 226947860

Gene position: 769220-770641 (Clockwise)

Preceding gene: 226947859

Following gene: 226947862

Centisome position: 18.51

GC content: 24.19

Gene sequence:

>1422_bases
ATGAAAATAAGAGATAAATTTATAACTGGTTTAATATTTATACTGATAGTTTCTATGATAGTAATGAATGTGGCAATAAC
ACATGTTTTAAAGTCTAATATGGAAAATAGTATAAATAATTTTTTAAAACAAGTAATGAATAGCACTCACGAGTATGTAA
AGTACAACCTTGTAACTAATAGTTTTAAGGATAAAAAAGAATCATTGGTTGAAGAAGGGAATTACATAATAAAACATATA
TCTTTAAATTATGAATGTAAATGTGATATAAGAGATATAAATTATATATTGATAGAAGGAAATGTTTCAGAGGAATTCAG
AAGCATAACAAAAAAAAGTAAAAAAATAGCAATGGATGGTAAAGCCGTAGTAGATTTGAAATATAGATATAATGGAGTAG
ATGCAATACTAACTTATCCTATATATATAGATAATGAATATGTGGGGATCATAAGCATAGTAAAGAATTATGACTCGAAA
TATAAGGATTATAAAAATACTATAAATATTATAAATATTATAGAACTTGGAACATTTATAGTTATATTTATATTTTTATT
TTTAAGAACAAATAAAATTACTAAACCAATTACAGAATTAACAGATGCAATAAAAAAACTAGGATATGGTGATTATAATA
TTTCCATTGCTGAACATGGAAAAGATGAAGTAGCAATATTAGCAAGAGAATTTATAAATATGAGAGATAAAATAAAAGAA
CAAATAGAAACTATTGAATCAGAAAAGAACAAAGTTTATAAATTAGAAAAAGGAAGAAAGGAATTTTTTAATAGTGTAAC
CCATGAATTAAAAACACCATTGACAGCTATATCAGGATATGCTGAGTTACTACTTACGGGAATGGTCAAGGATAAAGACT
TTGATAAAAGAGCTATTGAAAGAATATATTCAGAAAGTGATAGGCTTCATAACTTAGTATTAGATCTTATAGAGGTGTCT
AAAGGTATGAGTGTTATTAAAGAAGAACTTAATTATGTTGACATGAAAGAGCTTATTATTCAAAGTTGTAATGATATGAA
TATAAAGGCTAATAAATATTCCTTAAAAATAATACAAAATATAAGTGAAGGAACAGTTAAGGTACAACAGGATAAAATTA
GGCAAGTACTAATCAATATAATTGATAATGCAATAAAATATTCCTATGGGGGAAATGAGATATATGTTAATTCCTACATA
TCAGACAATAAATATATTATTGAAGTAATAAATAATAGTAATCCAATCCCAGATGAAATATTTAATAGTATATTTGAACC
TTTTATAAAATCTAATAATGATAATAAAGATAGTAGAGGCCTTGGTTTATATTTATGTAATGAAATAGTTAAAGAGCATG
ACGGTGAAATAACCATAGAAAATGGATCTGTAGTAAAAGTAAAAATTAATTTAATAATTTAA

Upstream 100 bases:

>100_bases
GACGTTCATGTTAGAAGATTAAGAAGTAAGCTTGATAAGGAGAATGCAACATCTATTATAGACACTGTTTTTGGAATAGG
ATATGTAATGAGGTAGCCTT

Downstream 100 bases:

>100_bases
AAATATGAAAAGCGCACTAATCCTCATGATATAGGTGCTTTTTATTTTTATTATTAATTTTTATATGAATTTCAAAAAAT
TTAATTTTAATAATTAAGTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKIRDKFITGLIFILIVSMIVMNVAITHVLKSNMENSINNFLKQVMNSTHEYVKYNLVTNSFKDKKESLVEEGNYIIKHI
SLNYECKCDIRDINYILIEGNVSEEFRSITKKSKKIAMDGKAVVDLKYRYNGVDAILTYPIYIDNEYVGIISIVKNYDSK
YKDYKNTINIINIIELGTFIVIFIFLFLRTNKITKPITELTDAIKKLGYGDYNISIAEHGKDEVAILAREFINMRDKIKE
QIETIESEKNKVYKLEKGRKEFFNSVTHELKTPLTAISGYAELLLTGMVKDKDFDKRAIERIYSESDRLHNLVLDLIEVS
KGMSVIKEELNYVDMKELIIQSCNDMNIKANKYSLKIIQNISEGTVKVQQDKIRQVLINIIDNAIKYSYGGNEIYVNSYI
SDNKYIIEVINNSNPIPDEIFNSIFEPFIKSNNDNKDSRGLGLYLCNEIVKEHDGEITIENGSVVKVKINLII

Sequences:

>Translated_473_residues
MKIRDKFITGLIFILIVSMIVMNVAITHVLKSNMENSINNFLKQVMNSTHEYVKYNLVTNSFKDKKESLVEEGNYIIKHI
SLNYECKCDIRDINYILIEGNVSEEFRSITKKSKKIAMDGKAVVDLKYRYNGVDAILTYPIYIDNEYVGIISIVKNYDSK
YKDYKNTINIINIIELGTFIVIFIFLFLRTNKITKPITELTDAIKKLGYGDYNISIAEHGKDEVAILAREFINMRDKIKE
QIETIESEKNKVYKLEKGRKEFFNSVTHELKTPLTAISGYAELLLTGMVKDKDFDKRAIERIYSESDRLHNLVLDLIEVS
KGMSVIKEELNYVDMKELIIQSCNDMNIKANKYSLKIIQNISEGTVKVQQDKIRQVLINIIDNAIKYSYGGNEIYVNSYI
SDNKYIIEVINNSNPIPDEIFNSIFEPFIKSNNDNKDSRGLGLYLCNEIVKEHDGEITIENGSVVKVKINLII
>Mature_473_residues
MKIRDKFITGLIFILIVSMIVMNVAITHVLKSNMENSINNFLKQVMNSTHEYVKYNLVTNSFKDKKESLVEEGNYIIKHI
SLNYECKCDIRDINYILIEGNVSEEFRSITKKSKKIAMDGKAVVDLKYRYNGVDAILTYPIYIDNEYVGIISIVKNYDSK
YKDYKNTINIINIIELGTFIVIFIFLFLRTNKITKPITELTDAIKKLGYGDYNISIAEHGKDEVAILAREFINMRDKIKE
QIETIESEKNKVYKLEKGRKEFFNSVTHELKTPLTAISGYAELLLTGMVKDKDFDKRAIERIYSESDRLHNLVLDLIEVS
KGMSVIKEELNYVDMKELIIQSCNDMNIKANKYSLKIIQNISEGTVKVQQDKIRQVLINIIDNAIKYSYGGNEIYVNSYI
SDNKYIIEVINNSNPIPDEIFNSIFEPFIKSNNDNKDSRGLGLYLCNEIVKEHDGEITIENGSVVKVKINLII

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=286, Percent_Identity=24.1258741258741, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082128, Length=300, Percent_Identity=24, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790861, Length=329, Percent_Identity=24.3161094224924, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788713, Length=247, Percent_Identity=25.5060728744939, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=24.4635193133047, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786600, Length=307, Percent_Identity=22.1498371335505, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081816, Length=333, Percent_Identity=21.3213213213213, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789808, Length=285, Percent_Identity=24.5614035087719, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790346, Length=283, Percent_Identity=19.7879858657244, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=24.6808510638298, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI145693157, Length=265, Percent_Identity=25.6603773584906, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54538; Mature: 54538

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRDKFITGLIFILIVSMIVMNVAITHVLKSNMENSINNFLKQVMNSTHEYVKYNLVTN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEHHC
SFKDKKESLVEEGNYIIKHISLNYECKCDIRDINYILIEGNVSEEFRSITKKSKKIAMDG
CHHHHHHHHHHCCCEEEEEEEECEEEEEEEEECEEEEEECCCHHHHHHHHHHHHHEEECC
KAVVDLKYRYNGVDAILTYPIYIDNEYVGIISIVKNYDSKYKDYKNTINIINIIELGTFI
CEEEEEEEEECCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIFIFLFLRTNKITKPITELTDAIKKLGYGDYNISIAEHGKDEVAILAREFINMRDKIKE
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
QIETIESEKNKVYKLEKGRKEFFNSVTHELKTPLTAISGYAELLLTGMVKDKDFDKRAIE
HHHHHHHHCHHEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
RIYSESDRLHNLVLDLIEVSKGMSVIKEELNYVDMKELIIQSCNDMNIKANKYSLKIIQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCEEEHHHHC
ISEGTVKVQQDKIRQVLINIIDNAIKYSYGGNEIYVNSYISDNKYIIEVINNSNPIPDEI
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCCCHHHH
FNSIFEPFIKSNNDNKDSRGLGLYLCNEIVKEHDGEITIENGSVVKVKINLII
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEC
>Mature Secondary Structure
MKIRDKFITGLIFILIVSMIVMNVAITHVLKSNMENSINNFLKQVMNSTHEYVKYNLVTN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEHHC
SFKDKKESLVEEGNYIIKHISLNYECKCDIRDINYILIEGNVSEEFRSITKKSKKIAMDG
CHHHHHHHHHHCCCEEEEEEEECEEEEEEEEECEEEEEECCCHHHHHHHHHHHHHEEECC
KAVVDLKYRYNGVDAILTYPIYIDNEYVGIISIVKNYDSKYKDYKNTINIINIIELGTFI
CEEEEEEEEECCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIFIFLFLRTNKITKPITELTDAIKKLGYGDYNISIAEHGKDEVAILAREFINMRDKIKE
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
QIETIESEKNKVYKLEKGRKEFFNSVTHELKTPLTAISGYAELLLTGMVKDKDFDKRAIE
HHHHHHHHCHHEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
RIYSESDRLHNLVLDLIEVSKGMSVIKEELNYVDMKELIIQSCNDMNIKANKYSLKIIQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCEEEHHHHC
ISEGTVKVQQDKIRQVLINIIDNAIKYSYGGNEIYVNSYISDNKYIIEVINNSNPIPDEI
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCCCHHHH
FNSIFEPFIKSNNDNKDSRGLGLYLCNEIVKEHDGEITIENGSVVKVKINLII
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]