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The map label for this gene is yfiK [H]

Identifier: 226947735

GI number: 226947735

Start: 629377

End: 630009

Strand: Direct

Name: yfiK [H]

Synonym: CLM_0584

Alternate gene names: 226947735

Gene position: 629377-630009 (Clockwise)

Preceding gene: 226947734

Following gene: 226947736

Centisome position: 15.15

GC content: 27.65

Gene sequence:

>633_bases
GTGATTAAGGTTTTAATTGCAGATGACGATGTTTTTATAAGAGAAAGCTTAAGTATAATATTAGATATGGATGAAGATAT
AAAAATAGAGAAAACCGTAGAAAATGGATTGCAAGCATTAGAATATTGTAGAAATAATAAAATAGATATAGCACTTTTAG
ATATAAGAATGCCTGTAATGAACGGAGTAGAAGCTACAAAAATAATAAGTAGAGAAGGTCATGGGAAGGTTTTAATATTA
ACTACTTTTGATGAAGATGATTACATAAGAGAAGCTCTTCATTTTGGGGCTAAGGGATATATTTTAAAAAACAATACTCC
AGATACTATAATAGATACTATAAAAATGGTTTATAAAGGTAATTCTGTTATACAAGATGTAGTTATGGATAAAGTACTTA
ATTCCTGTAATTCCAATATTAAAAATATTGATAAAGATATGTTTTCAAGAAGGGAAATAGATGTAATAGAGGCTATATCA
GAAGGCTTATCTAATAAAGAAATATCAGCAAAATTATTTATTTCAGAGGGAACTGTAAAAAATTATATAAGTTCTATTTT
ATCTAAGACTGGCTTAGAACATAGGACTCAAATAGCCATATACCATTTGAAGGGGGAACTAAGTGATTCTTAG

Upstream 100 bases:

>100_bases
TTATTTCCTATATATTTTAAGATTGTATGTATTTAAAATAGTATTGTAATTAGAAAGTGTATTTAAAATATAATTAATGT
ATAAAAAAGGGGGGAATAGA

Downstream 100 bases:

>100_bases
GTTTAGATAGAATTTGCTTACAAGGATACCCGCTCCAGTTAAATATATTACTTTCGTCATACATATAAAATGACCTTTTA
CACTGATGTGAAGGGTCATT

Product: LuxR family DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIKVLIADDDVFIRESLSIILDMDEDIKIEKTVENGLQALEYCRNNKIDIALLDIRMPVMNGVEATKIISREGHGKVLIL
TTFDEDDYIREALHFGAKGYILKNNTPDTIIDTIKMVYKGNSVIQDVVMDKVLNSCNSNIKNIDKDMFSRREIDVIEAIS
EGLSNKEISAKLFISEGTVKNYISSILSKTGLEHRTQIAIYHLKGELSDS

Sequences:

>Translated_210_residues
MIKVLIADDDVFIRESLSIILDMDEDIKIEKTVENGLQALEYCRNNKIDIALLDIRMPVMNGVEATKIISREGHGKVLIL
TTFDEDDYIREALHFGAKGYILKNNTPDTIIDTIKMVYKGNSVIQDVVMDKVLNSCNSNIKNIDKDMFSRREIDVIEAIS
EGLSNKEISAKLFISEGTVKNYISSILSKTGLEHRTQIAIYHLKGELSDS
>Mature_210_residues
MIKVLIADDDVFIRESLSIILDMDEDIKIEKTVENGLQALEYCRNNKIDIALLDIRMPVMNGVEATKIISREGHGKVLIL
TTFDEDDYIREALHFGAKGYILKNNTPDTIIDTIKMVYKGNSVIQDVVMDKVLNSCNSNIKNIDKDMFSRREIDVIEAIS
EGLSNKEISAKLFISEGTVKNYISSILSKTGLEHRTQIAIYHLKGELSDS

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=207, Percent_Identity=30.4347826086957, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1788521, Length=207, Percent_Identity=29.951690821256, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=25, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1787473, Length=201, Percent_Identity=28.3582089552239, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1786747, Length=192, Percent_Identity=28.6458333333333, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1788712, Length=177, Percent_Identity=26.5536723163842, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082052, Length=103, Percent_Identity=34.9514563106796, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23670; Mature: 23670

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVLIADDDVFIRESLSIILDMDEDIKIEKTVENGLQALEYCRNNKIDIALLDIRMPVM
CEEEEEECCCEEHHCCEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEECHHH
NGVEATKIISREGHGKVLILTTFDEDDYIREALHFGAKGYILKNNTPDTIIDTIKMVYKG
CCCHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCC
NSVIQDVVMDKVLNSCNSNIKNIDKDMFSRREIDVIEAISEGLSNKEISAKLFISEGTVK
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEECCCHH
NYISSILSKTGLEHRTQIAIYHLKGELSDS
HHHHHHHHHCCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MIKVLIADDDVFIRESLSIILDMDEDIKIEKTVENGLQALEYCRNNKIDIALLDIRMPVM
CEEEEEECCCEEHHCCEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEECHHH
NGVEATKIISREGHGKVLILTTFDEDDYIREALHFGAKGYILKNNTPDTIIDTIKMVYKG
CCCHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCC
NSVIQDVVMDKVLNSCNSNIKNIDKDMFSRREIDVIEAISEGLSNKEISAKLFISEGTVK
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEECCCHH
NYISSILSKTGLEHRTQIAIYHLKGELSDS
HHHHHHHHHCCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]