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The map label for this gene is cadA [H]

Identifier: 226947714

GI number: 226947714

Start: 606581

End: 608797

Strand: Direct

Name: cadA [H]

Synonym: CLM_0561

Alternate gene names: 226947714

Gene position: 606581-608797 (Clockwise)

Preceding gene: 226947713

Following gene: 226947715

Centisome position: 14.6

GC content: 30.04

Gene sequence:

>2217_bases
ATGATTATAAAAAAGAAATTGCTTTTAAAAGGCTTACATTGTGCTAATTGTGCAGCAAAAATAGAACGAGCTGTGCAAAA
GCTAAATATAATAGAGGAAGCTAACTATAATTTTAATAATTCAACTTTAATTATTAACTTAGAAGAAACTCATAAAGACA
GCATTATAAAAACAATACAAGAAATAGTAGATAGAATAGAACCTGGGGTAAAGGTAGTAGATAAAGAAAATTTAAAAAGA
ACGGTAGTGCATGATCCTGTTAAAAATAGTAATAATCTAAAGATGCAGAATAATAAAGAAAAAAATTTAAAATTAGATAA
AAAAGAAAATAGTTATGAGCATAGTCATGGACACAGTCATGAAGGAGAAGATAGTGACGAGTTAGAAAAGAAGACTCTTA
TAAGAATTATTTCAGGAGTGGTTTTATTAATTTTAGCTACCGTATTGAAATCTAGAGAAACTTTGTCTATAGGCCTATAT
TTAGCTTCATATGCATTAATTGGAGGTAAGGTAATATTAAGTTCTATAAGAAATATATCTAAAGGACAAGTATTTGATGA
AAACTTTTTAATGGCTGTTGCAACTGCAGCGGCTATAGCAGTAAAACAGTATCCAGAAGCAGTAGCAGTTATGCTATTTT
ATGAAGTAGGGGAGTTCTTGCAGGATAAAGCTGTTAATAAATCTAGAAAATCCATAACAGCACTTATGAATATAAGACCT
GACTATGCCAATTTAGTTAAGGGTGAAGATATAGAAGTAGTTTCTCCAGAAGATATTAATATAGATGATATTATAATGGT
AAAACCAGGAGAAAAAATTCCTCTAGATGGTATAGTTGTAGAAGGACAATCTTCTGTAGATACATCAGCTATAACAGGTG
AATCTTTAATAAGTGAAGTTTCAAAAGATAGTAATGTATTAAGTGGATATATAAACAAAAATGGTGTTATAAAAATAAAA
GTTACAAAAACCTTTGAAGAATCTACAGTGTCCAAAATATTGGAATTAACAGAAAACGCATCAGCTACTAAAGCAAATAC
AGAAAAATTTATAACAAAGTTCGCCAGATATTATACTCCTGTAGTTGTTTTTGCTGCTTTAGCTTTAGCAGTAATTCCTA
CACTAATACTAAAAGATCCAGATATATCTAAATGGATTTATAGAGCGGCTGTATTTTTAGTAGTATCTTGTCCATGTGCT
TTAGTAATATCAATACCTTTAAGCTTCTTTGCTGGTATTGGAGGAGCATCTAAAAAAGGTGTGTTAATAAAAACAGGAAC
AGCATTAGAAGCTCTAAATGATGCAGATACAATAGTATTTGATAAAACAGGAACTCTAACTAAAGGTGTGTTTAAAGTAT
CAAAAATTGAGTCAGAAGAAGGAGTAAATACAGAAGAATTAATAGAATATGCTGCTTATGTAGAAAGTTATTCTAATCAC
CCTATCGCAAAATCTATACTAAAGTATTATGAAAAAACCATAGATAACAAAAGAATAGAAGGTTATGAAGAAATAGTTGC
AAGAGGTGTAACTGCTTATATTGATGGCAAAAAAGTTTATGCTGGTAATAATAAATTAATGGAAGAATTAAATATTAACT
ATAAAAAAGCTCCAAGGGATGGGGTAATCCTTTATATAGCATTAGAAGATAAGTATATAGGTTATATAGTTATAAACGAC
GAAATAAAGAAAGATTCAAAAGAAACAATAAAATCATTAAAAGATATAGGCATAAAAAAAGCAGCTATGCTTACAGGAGA
TAGAAAATCTACAGCTAATAATATAGGGACTTTTCTAGGAATGGATGAAATATACTCAGAACTTTTACCACAGGAAAAGG
TAGAAAAGATGCAATCCTTAAAGTCCAAAACATCTAAAGATGGGAAAATTGTTTTCGTAGGGGATGGAGTAAATGATGCT
CCAGTATTAGCTATGTCAGATATAGGGGTATCCATGGGAGGCTTAGGATCTGATGCAGCCATAGAAGCATCTGATTTAGT
TTTAATGTCTGATGAGCCATCAAAATTAGTAGATGCTATAAAAATAGCAAGAAAAACTCATAAAATAGTATGGCAAAATA
TAATTATAGTTTTAATAATTAAGTTTGCAGTATTAGCTCTAGCTGTAATGGGAAAAAGTACTATGTGGATGGCTGTCTTT
GCCGATGTAGGTGTGGCTTTAATAGCAGTAATAAATGCATTAAGAATACTAAAATAA

Upstream 100 bases:

>100_bases
ACTAGAACACATAATGCATAGATAAAATTAAGTTTAATATTTTATTGTAATTTTAGAAATTTTAATTTTTTAATAACTAT
TTTAGAAGGATGGTGGGATT

Downstream 100 bases:

>100_bases
AAATTTACTAAAATAATAACTTTCTAAAGAACAATAGGAACGAATAATTTTAAAGAAAAATATTTAATTATATTTATATA
GTTTCAGTTAAGTAATTTTA

Product: cadmium-exporting ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
MIIKKKLLLKGLHCANCAAKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQEIVDRIEPGVKVVDKENLKR
TVVHDPVKNSNNLKMQNNKEKNLKLDKKENSYEHSHGHSHEGEDSDELEKKTLIRIISGVVLLILATVLKSRETLSIGLY
LASYALIGGKVILSSIRNISKGQVFDENFLMAVATAAAIAVKQYPEAVAVMLFYEVGEFLQDKAVNKSRKSITALMNIRP
DYANLVKGEDIEVVSPEDINIDDIIMVKPGEKIPLDGIVVEGQSSVDTSAITGESLISEVSKDSNVLSGYINKNGVIKIK
VTKTFEESTVSKILELTENASATKANTEKFITKFARYYTPVVVFAALALAVIPTLILKDPDISKWIYRAAVFLVVSCPCA
LVISIPLSFFAGIGGASKKGVLIKTGTALEALNDADTIVFDKTGTLTKGVFKVSKIESEEGVNTEELIEYAAYVESYSNH
PIAKSILKYYEKTIDNKRIEGYEEIVARGVTAYIDGKKVYAGNNKLMEELNINYKKAPRDGVILYIALEDKYIGYIVIND
EIKKDSKETIKSLKDIGIKKAAMLTGDRKSTANNIGTFLGMDEIYSELLPQEKVEKMQSLKSKTSKDGKIVFVGDGVNDA
PVLAMSDIGVSMGGLGSDAAIEASDLVLMSDEPSKLVDAIKIARKTHKIVWQNIIIVLIIKFAVLALAVMGKSTMWMAVF
ADVGVALIAVINALRILK

Sequences:

>Translated_738_residues
MIIKKKLLLKGLHCANCAAKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQEIVDRIEPGVKVVDKENLKR
TVVHDPVKNSNNLKMQNNKEKNLKLDKKENSYEHSHGHSHEGEDSDELEKKTLIRIISGVVLLILATVLKSRETLSIGLY
LASYALIGGKVILSSIRNISKGQVFDENFLMAVATAAAIAVKQYPEAVAVMLFYEVGEFLQDKAVNKSRKSITALMNIRP
DYANLVKGEDIEVVSPEDINIDDIIMVKPGEKIPLDGIVVEGQSSVDTSAITGESLISEVSKDSNVLSGYINKNGVIKIK
VTKTFEESTVSKILELTENASATKANTEKFITKFARYYTPVVVFAALALAVIPTLILKDPDISKWIYRAAVFLVVSCPCA
LVISIPLSFFAGIGGASKKGVLIKTGTALEALNDADTIVFDKTGTLTKGVFKVSKIESEEGVNTEELIEYAAYVESYSNH
PIAKSILKYYEKTIDNKRIEGYEEIVARGVTAYIDGKKVYAGNNKLMEELNINYKKAPRDGVILYIALEDKYIGYIVIND
EIKKDSKETIKSLKDIGIKKAAMLTGDRKSTANNIGTFLGMDEIYSELLPQEKVEKMQSLKSKTSKDGKIVFVGDGVNDA
PVLAMSDIGVSMGGLGSDAAIEASDLVLMSDEPSKLVDAIKIARKTHKIVWQNIIIVLIIKFAVLALAVMGKSTMWMAVF
ADVGVALIAVINALRILK
>Mature_738_residues
MIIKKKLLLKGLHCANCAAKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQEIVDRIEPGVKVVDKENLKR
TVVHDPVKNSNNLKMQNNKEKNLKLDKKENSYEHSHGHSHEGEDSDELEKKTLIRIISGVVLLILATVLKSRETLSIGLY
LASYALIGGKVILSSIRNISKGQVFDENFLMAVATAAAIAVKQYPEAVAVMLFYEVGEFLQDKAVNKSRKSITALMNIRP
DYANLVKGEDIEVVSPEDINIDDIIMVKPGEKIPLDGIVVEGQSSVDTSAITGESLISEVSKDSNVLSGYINKNGVIKIK
VTKTFEESTVSKILELTENASATKANTEKFITKFARYYTPVVVFAALALAVIPTLILKDPDISKWIYRAAVFLVVSCPCA
LVISIPLSFFAGIGGASKKGVLIKTGTALEALNDADTIVFDKTGTLTKGVFKVSKIESEEGVNTEELIEYAAYVESYSNH
PIAKSILKYYEKTIDNKRIEGYEEIVARGVTAYIDGKKVYAGNNKLMEELNINYKKAPRDGVILYIALEDKYIGYIVIND
EIKKDSKETIKSLKDIGIKKAAMLTGDRKSTANNIGTFLGMDEIYSELLPQEKVEKMQSLKSKTSKDGKIVFVGDGVNDA
PVLAMSDIGVSMGGLGSDAAIEASDLVLMSDEPSKLVDAIKIARKTHKIVWQNIIIVLIIKFAVLALAVMGKSTMWMAVF
ADVGVALIAVINALRILK

Specific function: Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. Does not seem to transport copper [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=595, Percent_Identity=27.8991596638655, Blast_Score=231, Evalue=2e-60,
Organism=Homo sapiens, GI55743073, Length=559, Percent_Identity=27.906976744186, Blast_Score=208, Evalue=2e-53,
Organism=Homo sapiens, GI115529486, Length=319, Percent_Identity=29.4670846394984, Blast_Score=155, Evalue=2e-37,
Organism=Escherichia coli, GI1789879, Length=744, Percent_Identity=29.3010752688172, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1786691, Length=731, Percent_Identity=25.9917920656635, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1786914, Length=551, Percent_Identity=25.7713248638838, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI2367363, Length=611, Percent_Identity=22.5859247135843, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17556548, Length=577, Percent_Identity=29.4627383015598, Blast_Score=195, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI71997275, Length=191, Percent_Identity=28.2722513089005, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71997262, Length=191, Percent_Identity=28.2722513089005, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6320475, Length=545, Percent_Identity=28.4403669724771, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6319772, Length=615, Percent_Identity=27.3170731707317, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325221, Length=567, Percent_Identity=23.8095238095238, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321430, Length=552, Percent_Identity=23.3695652173913, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321271, Length=218, Percent_Identity=31.651376146789, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321432, Length=185, Percent_Identity=30.8108108108108, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221329854, Length=308, Percent_Identity=31.1688311688312, Blast_Score=142, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24668708, Length=158, Percent_Identity=30.379746835443, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24668704, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281366617, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI161085803, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24668696, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3; =3.6.3.5 [H]

Molecular weight: Translated: 80977; Mature: 80977

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKKKLLLKGLHCANCAAKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQ
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHH
EIVDRIEPGVKVVDKENLKRTVVHDPVKNSNNLKMQNNKEKNLKLDKKENSYEHSHGHSH
HHHHHHCCCCEEECHHCCHHHHHCCCCCCCCCEEECCCCCCCCEECCCCCCCCCCCCCCC
EGEDSDELEKKTLIRIISGVVLLILATVLKSRETLSIGLYLASYALIGGKVILSSIRNIS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KGQVFDENFLMAVATAAAIAVKQYPEAVAVMLFYEVGEFLQDKAVNKSRKSITALMNIRP
CCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCC
DYANLVKGEDIEVVSPEDINIDDIIMVKPGEKIPLDGIVVEGQSSVDTSAITGESLISEV
CHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
SKDSNVLSGYINKNGVIKIKVTKTFEESTVSKILELTENASATKANTEKFITKFARYYTP
HCCCCHHHHEECCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VVVFAALALAVIPTLILKDPDISKWIYRAAVFLVVSCPCALVISIPLSFFAGIGGASKKG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VLIKTGTALEALNDADTIVFDKTGTLTKGVFKVSKIESEEGVNTEELIEYAAYVESYSNH
EEEECCCHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
PIAKSILKYYEKTIDNKRIEGYEEIVARGVTAYIDGKKVYAGNNKLMEELNINYKKAPRD
HHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCEEEECCHHHHHHHCCCCCCCCCC
GVILYIALEDKYIGYIVINDEIKKDSKETIKSLKDIGIKKAAMLTGDRKSTANNIGTFLG
CEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHEECCCCHHHHHHHHHHCC
MDEIYSELLPQEKVEKMQSLKSKTSKDGKIVFVGDGVNDAPVLAMSDIGVSMGGLGSDAA
HHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCC
IEASDLVLMSDEPSKLVDAIKIARKTHKIVWQNIIIVLIIKFAVLALAVMGKSTMWMAVF
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ADVGVALIAVINALRILK
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIIKKKLLLKGLHCANCAAKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQ
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHH
EIVDRIEPGVKVVDKENLKRTVVHDPVKNSNNLKMQNNKEKNLKLDKKENSYEHSHGHSH
HHHHHHCCCCEEECHHCCHHHHHCCCCCCCCCEEECCCCCCCCEECCCCCCCCCCCCCCC
EGEDSDELEKKTLIRIISGVVLLILATVLKSRETLSIGLYLASYALIGGKVILSSIRNIS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KGQVFDENFLMAVATAAAIAVKQYPEAVAVMLFYEVGEFLQDKAVNKSRKSITALMNIRP
CCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCC
DYANLVKGEDIEVVSPEDINIDDIIMVKPGEKIPLDGIVVEGQSSVDTSAITGESLISEV
CHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
SKDSNVLSGYINKNGVIKIKVTKTFEESTVSKILELTENASATKANTEKFITKFARYYTP
HCCCCHHHHEECCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VVVFAALALAVIPTLILKDPDISKWIYRAAVFLVVSCPCALVISIPLSFFAGIGGASKKG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VLIKTGTALEALNDADTIVFDKTGTLTKGVFKVSKIESEEGVNTEELIEYAAYVESYSNH
EEEECCCHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
PIAKSILKYYEKTIDNKRIEGYEEIVARGVTAYIDGKKVYAGNNKLMEELNINYKKAPRD
HHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCEEEECCHHHHHHHCCCCCCCCCC
GVILYIALEDKYIGYIVINDEIKKDSKETIKSLKDIGIKKAAMLTGDRKSTANNIGTFLG
CEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHEECCCCHHHHHHHHHHCC
MDEIYSELLPQEKVEKMQSLKSKTSKDGKIVFVGDGVNDAPVLAMSDIGVSMGGLGSDAA
HHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCC
IEASDLVLMSDEPSKLVDAIKIARKTHKIVWQNIIIVLIIKFAVLALAVMGKSTMWMAVF
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ADVGVALIAVINALRILK
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]