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The map label for this gene is rnhA [H]

Identifier: 226947635

GI number: 226947635

Start: 512819

End: 513268

Strand: Direct

Name: rnhA [H]

Synonym: CLM_0472

Alternate gene names: 226947635

Gene position: 512819-513268 (Clockwise)

Preceding gene: 226947634

Following gene: 226947636

Centisome position: 12.34

GC content: 28.22

Gene sequence:

>450_bases
ATGAAAAAAGTAATAATATACACTGATGGAGCTTGTAGAGGAAATGGGCAAGAAAATACTATAGGGGCTTATGGAATAGT
TTTAATGTATGGTGAACATAAAAAAGAAATAAAAAAAGCTTTTAGGGATACAACTAATAATATTATGGAATTATCTGCAG
TGGTAGAGGCACTTTCTTTATTAAGAGAACCTTGCAGCGTAGAACTTTATAGTGATTCTGCTTATGTTATAAATGCCATA
AATCAAAAATGGCTAGATAATTGGAAGAAAAATAATTGGAAAACTGCTTCTAAATCTCCAGTTAAGAATAAAGAATTGTG
GGAAAAACTAGATGAATTATTAAAAAAACATTCTGTTAAGTTTATAAAAGTAAAGGGTCATTCAGATAATGAGTATAATA
ATAGATGTGATAAATTAGCAAATGAAGCCATGGATGAATTTAATGTTTAA

Upstream 100 bases:

>100_bases
AATAATATTGTAGGGTTTATAAAGGGAAAATGTGATGGAAAAAGTAATTTAAAAGCACATAAGCAAGAAAATAAGGCTTA
TGAGGAGGTGGCATCTGTAG

Downstream 100 bases:

>100_bases
TATTTTACAGAAATGCATATCCTACACTTAGTATTAAAAACAAATAAAGGAGAGTGTAAAATATGAACAATAGATTTTTA
TCAGGTATTGCTGCAGGGGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKKVIIYTDGACRGNGQENTIGAYGIVLMYGEHKKEIKKAFRDTTNNIMELSAVVEALSLLREPCSVELYSDSAYVINAI
NQKWLDNWKKNNWKTASKSPVKNKELWEKLDELLKKHSVKFIKVKGHSDNEYNNRCDKLANEAMDEFNV

Sequences:

>Translated_149_residues
MKKVIIYTDGACRGNGQENTIGAYGIVLMYGEHKKEIKKAFRDTTNNIMELSAVVEALSLLREPCSVELYSDSAYVINAI
NQKWLDNWKKNNWKTASKSPVKNKELWEKLDELLKKHSVKFIKVKGHSDNEYNNRCDKLANEAMDEFNV
>Mature_149_residues
MKKVIIYTDGACRGNGQENTIGAYGIVLMYGEHKKEIKKAFRDTTNNIMELSAVVEALSLLREPCSVELYSDSAYVINAI
NQKWLDNWKKNNWKTASKSPVKNKELWEKLDELLKKHSVKFIKVKGHSDNEYNNRCDKLANEAMDEFNV

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=145, Percent_Identity=31.0344827586207, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1786408, Length=143, Percent_Identity=47.5524475524476, Blast_Score=132, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI115532542, Length=145, Percent_Identity=32.4137931034483, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17505482, Length=136, Percent_Identity=36.7647058823529, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45552509, Length=147, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17137476, Length=147, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17146; Mature: 17146

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVIIYTDGACRGNGQENTIGAYGIVLMYGEHKKEIKKAFRDTTNNIMELSAVVEALSL
CCEEEEEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LREPCSVELYSDSAYVINAINQKWLDNWKKNNWKTASKSPVKNKELWEKLDELLKKHSVK
HHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
FIKVKGHSDNEYNNRCDKLANEAMDEFNV
EEEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKVIIYTDGACRGNGQENTIGAYGIVLMYGEHKKEIKKAFRDTTNNIMELSAVVEALSL
CCEEEEEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LREPCSVELYSDSAYVINAINQKWLDNWKKNNWKTASKSPVKNKELWEKLDELLKKHSVK
HHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
FIKVKGHSDNEYNNRCDKLANEAMDEFNV
EEEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA