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The map label for this gene is apbE [H]

Identifier: 226947608

GI number: 226947608

Start: 471848

End: 472882

Strand: Reverse

Name: apbE [H]

Synonym: CLM_0445

Alternate gene names: 226947608

Gene position: 472882-471848 (Counterclockwise)

Preceding gene: 226947613

Following gene: 226947607

Centisome position: 11.38

GC content: 29.37

Gene sequence:

>1035_bases
GTGAAAATTAAATATGTTACTATACTTCTTCTATGTATTTGTTTGCCTTTAGTTTTTGTAGGTTGCAATTCTAAATCAGA
GGAACCTGTATCTAGAGAAACCTATCTTATGGGAACTATAATAAATATTAAAGCCTATGGGAAAAATGCTGATAAAGCAG
TACAGGCTTCTGTGGATAAAATTTCTGATATAGAAAATAAAATGTCTCTTAATATATCTACTAGTGAAATTAATAAAATT
AATAAAAATGCAGGAATAGCTCCTGTTAAAGTAAGTAAAAATACTTTTGATGTAGTAAAAGCTTCATTAATATATAGTGA
AAAAACTAAAGGTTCCTTTGACATAACTGTAGAACCATTAGTATCTCTTTGGGGCATTGGAACAGACAAAGCAAGAATCC
CATCTAAGGATGAAACAAGCAATGCTTTAAAACTTATAAATTATAAAGATGTTATTATAAATGAAAAGGAATCTACTGTT
ATGCTTAAAAGGAAAGGTCAGGCTATAGATTTAGGTGCTATCGCTAAAGGATATACTGCAGATGAACTAAAAAAAGTTCT
TTTAAACTATAATGTTAGTTCTGCTTTTTTAAGTCTTGGAGGAAATGTATATGTTTTAGGAAATAAACCTGACAAAACCC
CTTGGAAAATAGGCGTTCAAAATCCTTTGGAACCTCGTGGAGATTATTTAGGTATAGTATCTGTTTCTGATAAGTCTGTA
GTTACCTCTGGAAATTACGAAAGATTTTTTGAAAGAAATGGGAAAAGATATCATCATATATTTGATACTAAGACAGGTTA
TCCTGCAGAAAAAGGGCTTATTAGTGTTTCTATAATATCAGATAAATCTATAGATGGTGATGCTTTATCTACAAGTGTCT
ATACTTTAGGCTTAGATGAAGGTAAAAAATTAATAGAAAGCCTAAATAATGTAGAAGCCGTATTTGTAACTAAGGACAAA
AAAGTTTATATAACCTCTGGGCTTAAAGATACATTTAAACTAACCAATACAGATTTTAAACTACAAAATAAATAA

Upstream 100 bases:

>100_bases
TATATAATTAAATATAGGGTTTATTAGAACTCTTATTTATAATAATTTAAAATTGATTATAAACTATTGTTGAAGACTAA
GGATTGGAGTTGAATTTATT

Downstream 100 bases:

>100_bases
CTCCTTTGAATAATTTATTTTTATATTTTAATAAGAATATATTAAAATATAAAAAAATTAATTTTTAAATTTAATAAATT
TTATTTTGTTATAAGATAAT

Product: apbE family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKIKYVTILLLCICLPLVFVGCNSKSEEPVSRETYLMGTIINIKAYGKNADKAVQASVDKISDIENKMSLNISTSEINKI
NKNAGIAPVKVSKNTFDVVKASLIYSEKTKGSFDITVEPLVSLWGIGTDKARIPSKDETSNALKLINYKDVIINEKESTV
MLKRKGQAIDLGAIAKGYTADELKKVLLNYNVSSAFLSLGGNVYVLGNKPDKTPWKIGVQNPLEPRGDYLGIVSVSDKSV
VTSGNYERFFERNGKRYHHIFDTKTGYPAEKGLISVSIISDKSIDGDALSTSVYTLGLDEGKKLIESLNNVEAVFVTKDK
KVYITSGLKDTFKLTNTDFKLQNK

Sequences:

>Translated_344_residues
MKIKYVTILLLCICLPLVFVGCNSKSEEPVSRETYLMGTIINIKAYGKNADKAVQASVDKISDIENKMSLNISTSEINKI
NKNAGIAPVKVSKNTFDVVKASLIYSEKTKGSFDITVEPLVSLWGIGTDKARIPSKDETSNALKLINYKDVIINEKESTV
MLKRKGQAIDLGAIAKGYTADELKKVLLNYNVSSAFLSLGGNVYVLGNKPDKTPWKIGVQNPLEPRGDYLGIVSVSDKSV
VTSGNYERFFERNGKRYHHIFDTKTGYPAEKGLISVSIISDKSIDGDALSTSVYTLGLDEGKKLIESLNNVEAVFVTKDK
KVYITSGLKDTFKLTNTDFKLQNK
>Mature_344_residues
MKIKYVTILLLCICLPLVFVGCNSKSEEPVSRETYLMGTIINIKAYGKNADKAVQASVDKISDIENKMSLNISTSEINKI
NKNAGIAPVKVSKNTFDVVKASLIYSEKTKGSFDITVEPLVSLWGIGTDKARIPSKDETSNALKLINYKDVIINEKESTV
MLKRKGQAIDLGAIAKGYTADELKKVLLNYNVSSAFLSLGGNVYVLGNKPDKTPWKIGVQNPLEPRGDYLGIVSVSDKSV
VTSGNYERFFERNGKRYHHIFDTKTGYPAEKGLISVSIISDKSIDGDALSTSVYTLGLDEGKKLIESLNNVEAVFVTKDK
KVYITSGLKDTFKLTNTDFKLQNK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=323, Percent_Identity=27.8637770897833, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37860; Mature: 37860

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKYVTILLLCICLPLVFVGCNSKSEEPVSRETYLMGTIINIKAYGKNADKAVQASVDK
CEEHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHH
ISDIENKMSLNISTSEINKINKNAGIAPVKVSKNTFDVVKASLIYSEKTKGSFDITVEPL
HHHCCHHEEEEEEHHHHHHHCCCCCEEEEEECCCHHHHHHHHHEEECCCCCEEEEEHHHH
VSLWGIGTDKARIPSKDETSNALKLINYKDVIINEKESTVMLKRKGQAIDLGAIAKGYTA
HHHHCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEECCCEEEECCCCCCCCH
DELKKVLLNYNVSSAFLSLGGNVYVLGNKPDKTPWKIGVQNPLEPRGDYLGIVSVSDKSV
HHHHHHHHCCCHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCE
VTSGNYERFFERNGKRYHHIFDTKTGYPAEKGLISVSIISDKSIDGDALSTSVYTLGLDE
EECCCHHHHHHCCCCEEEEEEECCCCCCCHHCEEEEEEEECCCCCCCCEEEEEEEEEHHH
GKKLIESLNNVEAVFVTKDKKVYITSGLKDTFKLTNTDFKLQNK
HHHHHHHCCCCEEEEEECCCEEEEECCCCCEEEECCCCEEECCC
>Mature Secondary Structure
MKIKYVTILLLCICLPLVFVGCNSKSEEPVSRETYLMGTIINIKAYGKNADKAVQASVDK
CEEHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHH
ISDIENKMSLNISTSEINKINKNAGIAPVKVSKNTFDVVKASLIYSEKTKGSFDITVEPL
HHHCCHHEEEEEEHHHHHHHCCCCCEEEEEECCCHHHHHHHHHEEECCCCCEEEEEHHHH
VSLWGIGTDKARIPSKDETSNALKLINYKDVIINEKESTVMLKRKGQAIDLGAIAKGYTA
HHHHCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEECCCEEEECCCCCCCCH
DELKKVLLNYNVSSAFLSLGGNVYVLGNKPDKTPWKIGVQNPLEPRGDYLGIVSVSDKSV
HHHHHHHHCCCHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCE
VTSGNYERFFERNGKRYHHIFDTKTGYPAEKGLISVSIISDKSIDGDALSTSVYTLGLDE
EECCCHHHHHHCCCCEEEEEEECCCCCCCHHCEEEEEEEECCCCCCCCEEEEEEEEEHHH
GKKLIESLNNVEAVFVTKDKKVYITSGLKDTFKLTNTDFKLQNK
HHHHHHHCCCCEEEEEECCCEEEEECCCCCEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]