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The map label for this gene is yclK [H]

Identifier: 226947584

GI number: 226947584

Start: 443211

End: 444605

Strand: Direct

Name: yclK [H]

Synonym: CLM_0421

Alternate gene names: 226947584

Gene position: 443211-444605 (Clockwise)

Preceding gene: 226947583

Following gene: 226947585

Centisome position: 10.67

GC content: 26.45

Gene sequence:

>1395_bases
ATGAGTAAAATAAGTGTAAAGAGAAGATTAATGGTAAATTTTATATTAATTATAATAATAAGTGTTTTTATACTAGAATT
TTTATTAATTATTTTTACAAGGCATTATTATTATAATAATTTAGAAGATGCTATTTCTAATCAGATAAAAACTTCTGCAG
AATTTTATAACAAATATTTTTCAAATTCACCCCTAGAAGATAACGTATTAGATAATGTAGATGTATTTTGGAGGCAGACT
TCTGCACAAGTACAAATAATAGATACAAAGGGTAAAGTTTTAATGGATTCTATAGGAGTATCAAATAAAAGTCAAATAAA
AACTTCGGATATTCAAAAAGCATTAAAAGGAGAAAAAGGAGTCTGGATAGGCAAAGTTGACTATGATACTTCTGGTGTAA
TGGCAGTAACCTATCCATTAAAATCAGACAATCAAATAGTTGGAGTATTAAGATTTATAACCTCATTAAGATATGTAAAT
AGTGATATAGCAAGGATATCCTTAGGATTTTTACTTATTGGTTTAGTAGTAATTTTAATATCTGGTTTAGTTAGCTTGTT
TTTGGCTAATAGTATAACAGAACCTATAAAAGAATTAACTGACGTAGCAAATAAAATGGCTTCTGGTAACTTTAAGGTTA
AAAGTGAAAAAATGTTTGATGACGAGTTTGGAGAGTTGTCAGATACATTAAATTATATGGCAGATGAAATAGTAAAAAAA
GATCAGCTAAAAAATGAATTCATATCTTCTGTATCTCATGAGCTAAGAACTCCCTTGACATCTATAAAAGGCTGGGCAAT
AACATTAAATTCTAATGAAGTAGATAAAGAAATTTTAAAAGATGGATTAAAAATAATAGAAGATGAGAGCGAAAGACTAA
GCGGAATGGTAGAAGAATTATTAGATTTTTCAAAGTTTGTATCAGGAAAGATGAAATTAAATAAAGAAGAAATAAATATA
TGTGAAATAATAGATACAGTGAGAAAACAATTAGAACCCTATGCTTATAGAAATAGAATAAATTTTAAAGCAACTTGTAT
AAATAAACTACCTAATATATATGGCGATAGAAATAGAATAAAGCAAGTTTTAATAAATTTATTAGATAATGCTTTTAAGT
TTACTCCAGAAGGTGGATTAGTAGAGTTAAATACTAAATATGAAGAAGAATATATAACAATTGTTGTAAAAGATACAGGT
GTAGGAATTAATAAGGAAGATTTACCAAGAGTTAAAGAAAAATTTTATAAAGGTAATAGTGGCAAATCGAAAAATGGAAT
CGGGTTATCCATATGCGATGAAATAATAAAGCTTCATAATGGCGTATTACATATAGAAAGTGAAGAAAACAAAGGCACTA
CTATATCAATAAAATTACCAATGATAAAACTATGA

Upstream 100 bases:

>100_bases
AAGATTAAGATCAAAAATAGAAGAAACACCATCTGAGCCTAAATTTATAAAGACTGTATGGGGAACAGGTTATAGATGGC
AAAACACTTAAGGAGTTATT

Downstream 100 bases:

>100_bases
TACAAATTTATTTTATATTTAAATATAATTAATATTAATTTAATGTAACTAGGAGAATTCATATGAGAAAAAGTAAAATT
CTATTAATATTTATGATTTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSKISVKRRLMVNFILIIIISVFILEFLLIIFTRHYYYNNLEDAISNQIKTSAEFYNKYFSNSPLEDNVLDNVDVFWRQT
SAQVQIIDTKGKVLMDSIGVSNKSQIKTSDIQKALKGEKGVWIGKVDYDTSGVMAVTYPLKSDNQIVGVLRFITSLRYVN
SDIARISLGFLLIGLVVILISGLVSLFLANSITEPIKELTDVANKMASGNFKVKSEKMFDDEFGELSDTLNYMADEIVKK
DQLKNEFISSVSHELRTPLTSIKGWAITLNSNEVDKEILKDGLKIIEDESERLSGMVEELLDFSKFVSGKMKLNKEEINI
CEIIDTVRKQLEPYAYRNRINFKATCINKLPNIYGDRNRIKQVLINLLDNAFKFTPEGGLVELNTKYEEEYITIVVKDTG
VGINKEDLPRVKEKFYKGNSGKSKNGIGLSICDEIIKLHNGVLHIESEENKGTTISIKLPMIKL

Sequences:

>Translated_464_residues
MSKISVKRRLMVNFILIIIISVFILEFLLIIFTRHYYYNNLEDAISNQIKTSAEFYNKYFSNSPLEDNVLDNVDVFWRQT
SAQVQIIDTKGKVLMDSIGVSNKSQIKTSDIQKALKGEKGVWIGKVDYDTSGVMAVTYPLKSDNQIVGVLRFITSLRYVN
SDIARISLGFLLIGLVVILISGLVSLFLANSITEPIKELTDVANKMASGNFKVKSEKMFDDEFGELSDTLNYMADEIVKK
DQLKNEFISSVSHELRTPLTSIKGWAITLNSNEVDKEILKDGLKIIEDESERLSGMVEELLDFSKFVSGKMKLNKEEINI
CEIIDTVRKQLEPYAYRNRINFKATCINKLPNIYGDRNRIKQVLINLLDNAFKFTPEGGLVELNTKYEEEYITIVVKDTG
VGINKEDLPRVKEKFYKGNSGKSKNGIGLSICDEIIKLHNGVLHIESEENKGTTISIKLPMIKL
>Mature_463_residues
SKISVKRRLMVNFILIIIISVFILEFLLIIFTRHYYYNNLEDAISNQIKTSAEFYNKYFSNSPLEDNVLDNVDVFWRQTS
AQVQIIDTKGKVLMDSIGVSNKSQIKTSDIQKALKGEKGVWIGKVDYDTSGVMAVTYPLKSDNQIVGVLRFITSLRYVNS
DIARISLGFLLIGLVVILISGLVSLFLANSITEPIKELTDVANKMASGNFKVKSEKMFDDEFGELSDTLNYMADEIVKKD
QLKNEFISSVSHELRTPLTSIKGWAITLNSNEVDKEILKDGLKIIEDESERLSGMVEELLDFSKFVSGKMKLNKEEINIC
EIIDTVRKQLEPYAYRNRINFKATCINKLPNIYGDRNRIKQVLINLLDNAFKFTPEGGLVELNTKYEEEYITIVVKDTGV
GINKEDLPRVKEKFYKGNSGKSKNGIGLSICDEIIKLHNGVLHIESEENKGTTISIKLPMIKL

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=283, Percent_Identity=25.4416961130742, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI145693157, Length=243, Percent_Identity=31.2757201646091, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1790861, Length=392, Percent_Identity=25, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1786600, Length=235, Percent_Identity=27.6595744680851, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1788713, Length=250, Percent_Identity=29.2, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081816, Length=229, Percent_Identity=27.9475982532751, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789149, Length=272, Percent_Identity=28.3088235294118, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790346, Length=301, Percent_Identity=27.906976744186, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=26.4069264069264, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786783, Length=311, Percent_Identity=23.7942122186495, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=28.0172413793103, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=28.5123966942149, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788549, Length=217, Percent_Identity=25.8064516129032, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082128, Length=324, Percent_Identity=24.0740740740741, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788279, Length=276, Percent_Identity=23.9130434782609, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789403, Length=210, Percent_Identity=26.1904761904762, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789808, Length=276, Percent_Identity=23.5507246376812, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787894, Length=297, Percent_Identity=22.5589225589226, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790551, Length=319, Percent_Identity=22.5705329153605, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787374, Length=194, Percent_Identity=23.7113402061856, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=207, Percent_Identity=32.3671497584541, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52593; Mature: 52461

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKISVKRRLMVNFILIIIISVFILEFLLIIFTRHYYYNNLEDAISNQIKTSAEFYNKYF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SNSPLEDNVLDNVDVFWRQTSAQVQIIDTKGKVLMDSIGVSNKSQIKTSDIQKALKGEKG
CCCCCCCCHHCCHHHHEECCCCEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCC
VWIGKVDYDTSGVMAVTYPLKSDNQIVGVLRFITSLRYVNSDIARISLGFLLIGLVVILI
EEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
SGLVSLFLANSITEPIKELTDVANKMASGNFKVKSEKMFDDEFGELSDTLNYMADEIVKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCHHHHHHHHHHHHHHHHHHHH
DQLKNEFISSVSHELRTPLTSIKGWAITLNSNEVDKEILKDGLKIIEDESERLSGMVEEL
HHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LDFSKFVSGKMKLNKEEINICEIIDTVRKQLEPYAYRNRINFKATCINKLPNIYGDRNRI
HHHHHHHCCCCEECHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHCCHHCCCHHHH
KQVLINLLDNAFKFTPEGGLVELNTKYEEEYITIVVKDTGVGINKEDLPRVKEKFYKGNS
HHHHHHHHHHHHEECCCCCEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCC
GKSKNGIGLSICDEIIKLHNGVLHIESEENKGTTISIKLPMIKL
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
SKISVKRRLMVNFILIIIISVFILEFLLIIFTRHYYYNNLEDAISNQIKTSAEFYNKYF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SNSPLEDNVLDNVDVFWRQTSAQVQIIDTKGKVLMDSIGVSNKSQIKTSDIQKALKGEKG
CCCCCCCCHHCCHHHHEECCCCEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCC
VWIGKVDYDTSGVMAVTYPLKSDNQIVGVLRFITSLRYVNSDIARISLGFLLIGLVVILI
EEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
SGLVSLFLANSITEPIKELTDVANKMASGNFKVKSEKMFDDEFGELSDTLNYMADEIVKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCHHHHHHHHHHHHHHHHHHHH
DQLKNEFISSVSHELRTPLTSIKGWAITLNSNEVDKEILKDGLKIIEDESERLSGMVEEL
HHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LDFSKFVSGKMKLNKEEINICEIIDTVRKQLEPYAYRNRINFKATCINKLPNIYGDRNRI
HHHHHHHCCCCEECHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHCCHHCCCHHHH
KQVLINLLDNAFKFTPEGGLVELNTKYEEEYITIVVKDTGVGINKEDLPRVKEKFYKGNS
HHHHHHHHHHHHEECCCCCEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCC
GKSKNGIGLSICDEIIKLHNGVLHIESEENKGTTISIKLPMIKL
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]