Definition Clostridium botulinum A2 str. Kyoto chromosome, complete genome.
Accession NC_012563
Length 4,155,278

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The map label for this gene is yjbM [H]

Identifier: 226947406

GI number: 226947406

Start: 217304

End: 218092

Strand: Direct

Name: yjbM [H]

Synonym: CLM_0233

Alternate gene names: 226947406

Gene position: 217304-218092 (Clockwise)

Preceding gene: 226947404

Following gene: 226947413

Centisome position: 5.23

GC content: 27.63

Gene sequence:

>789_bases
ATGGCACTAGGTGAATGGAAAACTTTTTTAATACCATATAAGCAAGCAGTAGAGGAACTTAAGGTTAAATTTAGAAGTAT
AAGAAAAGAATATAGAGAAAAAAATGAATACTCTCCTATAGAATTTGTTACAGGGAGGGTAAAAGAAATCTCTAGTATCC
TAGAAAAAGCAAATAAATTCAATATACCGCTAGATAGAATTAGTTATGAAATGGAGGACATAGCTGGCATAAGAATAATG
TGTCAATTTACTAGCGATATAGAAAAGGTAGTAGAATTAGTAAGGCAGAGAAGAGATATGCAAATAATATATGAAAAAGA
TTATATAACTAATGTTAAAAATAGTGGATATAGAAGTTATCACATGATTATAAAATATCCTGTTAGTATGGCAGAAGGTC
AAAAGGAGATTTTAGCAGAATTTCAAATAAGAACATTAGCTATGAATTTTTGGGCTACTATAGAACATTCATTAAATTAT
AAATATAAACATCAAATACCTAAGGATATAAAGTTGAAGCTTAAATCAGCTGCAGATGCAGCATCTAATTTAGACAAATT
AATGATGGAAATAAAAGATGAAATAAAGGATGCACAAAAACTTTTTGAAGTAAAGTCAAGCTTAGTATCCTACATAATGG
ATAATATTTTAACTTTGCAATCTATGGGAAAAATTATAGATTCTAATAGATATCAAGAGCAATTGAACAAACTTATAGAA
GAAGGGGAAGTATTTGAATTAAATAGTCTTCTAAAATCTACAGAAAAGACTATAGAAATGTATAAATAA

Upstream 100 bases:

>100_bases
AATTATAATTATATTATAATATCAAAACAATATATAATGATAATAGGTAATGTAAAATAACTTACAAAATTCAAAGCATA
TATTGAAAAGGGTGAAGGGT

Downstream 100 bases:

>100_bases
TGTTTAAAGAGGATGTCTTAAAATAGATTTGATTTTAATATAGTAAATATAGAAAATACATGTAATAAGCATATGAATAA
GCTTTAGAAGTTCTTAATTT

Product: RelA/SpoT domain-containing protein

Products: NA

Alternate protein names: (p)ppGpp synthase yjbM; Small alarmone synthase 1; SAS 1 [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MALGEWKTFLIPYKQAVEELKVKFRSIRKEYREKNEYSPIEFVTGRVKEISSILEKANKFNIPLDRISYEMEDIAGIRIM
CQFTSDIEKVVELVRQRRDMQIIYEKDYITNVKNSGYRSYHMIIKYPVSMAEGQKEILAEFQIRTLAMNFWATIEHSLNY
KYKHQIPKDIKLKLKSAADAASNLDKLMMEIKDEIKDAQKLFEVKSSLVSYIMDNILTLQSMGKIIDSNRYQEQLNKLIE
EGEVFELNSLLKSTEKTIEMYK

Sequences:

>Translated_262_residues
MALGEWKTFLIPYKQAVEELKVKFRSIRKEYREKNEYSPIEFVTGRVKEISSILEKANKFNIPLDRISYEMEDIAGIRIM
CQFTSDIEKVVELVRQRRDMQIIYEKDYITNVKNSGYRSYHMIIKYPVSMAEGQKEILAEFQIRTLAMNFWATIEHSLNY
KYKHQIPKDIKLKLKSAADAASNLDKLMMEIKDEIKDAQKLFEVKSSLVSYIMDNILTLQSMGKIIDSNRYQEQLNKLIE
EGEVFELNSLLKSTEKTIEMYK
>Mature_261_residues
ALGEWKTFLIPYKQAVEELKVKFRSIRKEYREKNEYSPIEFVTGRVKEISSILEKANKFNIPLDRISYEMEDIAGIRIMC
QFTSDIEKVVELVRQRRDMQIIYEKDYITNVKNSGYRSYHMIIKYPVSMAEGQKEILAEFQIRTLAMNFWATIEHSLNYK
YKHQIPKDIKLKLKSAADAASNLDKLMMEIKDEIKDAQKLFEVKSSLVSYIMDNILTLQSMGKIIDSNRYQEQLNKLIEE
GEVFELNSLLKSTEKTIEMYK

Specific function: Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. YjbM has probably a

COG id: COG2357

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the relA/spoT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007685 [H]

Pfam domain/function: PF04607 RelA_SpoT [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 30917; Mature: 30786

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGEWKTFLIPYKQAVEELKVKFRSIRKEYREKNEYSPIEFVTGRVKEISSILEKANKF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
NIPLDRISYEMEDIAGIRIMCQFTSDIEKVVELVRQRRDMQIIYEKDYITNVKNSGYRSY
CCCHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCEE
HMIIKYPVSMAEGQKEILAEFQIRTLAMNFWATIEHSLNYKYKHQIPKDIKLKLKSAADA
EEHEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
ASNLDKLMMEIKDEIKDAQKLFEVKSSLVSYIMDNILTLQSMGKIIDSNRYQEQLNKLIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EGEVFELNSLLKSTEKTIEMYK
CCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALGEWKTFLIPYKQAVEELKVKFRSIRKEYREKNEYSPIEFVTGRVKEISSILEKANKF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
NIPLDRISYEMEDIAGIRIMCQFTSDIEKVVELVRQRRDMQIIYEKDYITNVKNSGYRSY
CCCHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCEE
HMIIKYPVSMAEGQKEILAEFQIRTLAMNFWATIEHSLNYKYKHQIPKDIKLKLKSAADA
EEHEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
ASNLDKLMMEIKDEIKDAQKLFEVKSSLVSYIMDNILTLQSMGKIIDSNRYQEQLNKLIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EGEVFELNSLLKSTEKTIEMYK
CCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]