Click here to switch to the map view.

The map label for this gene is dosC [H]

Identifier: 226947315

GI number: 226947315

Start: 114768

End: 115838

Strand: Direct

Name: dosC [H]

Synonym: CLM_0135

Alternate gene names: 226947315

Gene position: 114768-115838 (Clockwise)

Preceding gene: 226947313

Following gene: 226947316

Centisome position: 2.76

GC content: 19.42

Gene sequence:

>1071_bases
ATGTTATATGTTCTTATAATTACATTATTTTTAATTATGATAGTATATGAAAGTATCATCATAATTAATCTAAGAAAAAA
CATAAAAAAGTTAGAATATTCTAAGAGAATGATCTATAACATGACGAAAAATGTTAATAAAGAAAAAACAATAGACAAGG
TATATGAAATAATGTTAAAAACAGCTATAGATCTAATACCAAAAGCAACTCAAGGTAGTATTTTGACAATGGATAAGGAA
GAAAACTTTAATTATGTAGCAATACTAGGATATGATAATAAGTTAAAAAATATAAAACTAAACAAAAAAGAAGTTTATTT
ATATAAAATAAATAGATTTAAGGAAACGGCCATAATTGAAAATCCAATAAAATTAGATGAAAAAATTTTAAATAAAGAAA
AAATAAATGAATTTAAAGAGTTAGAAGCATTAAATATAAGTTGTATAATTTCAGCACCAATATATATAGATAATAAATTA
TTTGGCCAAATCAATATAGATTGTACAAAAGAAAATTATAAATTTAAAAAAGAAGATTTAGAGTTAATGGATCATATAAA
AGATGAATTACAATTAATAATAAAATCTTTTATGGTAAAAGAAGAATTAATACATAAAGCTAATTATGATGAATTGACAA
AAATTTTTAATAGAAGATATTTTAATGAAATAATGAAAAACGAAATAAAAGAAGGTAATAAGACTTTAGTACTAATTGAT
ATTGACAATTTCAAAACTATAAATGATATTTATGGACATAGTACTGGAGATTTTATTTTAAGGGTTTTTGCTAATTTAAT
AAAAAGATACTTAGGAGATAAAGATTTATTTTTTAGATTTGGTGGTGATGAGTTTATAGTATTATTTAATGACTTAGATG
ATAAAAGTGTTATAAGTTTTATGGAAAATATTAGACAAAAAATTAATAAAGATAAAATTAAAGGAATAAATATAGATTTT
AGCTATGGTATAAGTAAACTTAAAAACTATAATATGACAAACTATGAGGATATAATAAATTTAGCTGATAAAAATATGTA
TATTAATAAGAATAAGAAAATTATAGTATAA

Upstream 100 bases:

>100_bases
ATGTCTATAATTTCACCTAACATAGTAGCACCTCCTTTATATATTTTTTTATAAAAGACATATATTATTCATTAATTAAA
TAAATTAATTGGGGGTTAGT

Downstream 100 bases:

>100_bases
AAATAGTAACCTGTTAAAGCTGTTAGCATATATTCATAGATATAGAGGTGAATATATTGGATAGAAAACAGGTGGTATTT
AGAATATTTTTATCTATAGT

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKRMIYNMTKNVNKEKTIDKVYEIMLKTAIDLIPKATQGSILTMDKE
ENFNYVAILGYDNKLKNIKLNKKEVYLYKINRFKETAIIENPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKL
FGQINIDCTKENYKFKKEDLELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKTLVLID
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFNDLDDKSVISFMENIRQKINKDKIKGINIDF
SYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV

Sequences:

>Translated_356_residues
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKRMIYNMTKNVNKEKTIDKVYEIMLKTAIDLIPKATQGSILTMDKE
ENFNYVAILGYDNKLKNIKLNKKEVYLYKINRFKETAIIENPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKL
FGQINIDCTKENYKFKKEDLELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKTLVLID
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFNDLDDKSVISFMENIRQKINKDKIKGINIDF
SYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV
>Mature_356_residues
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKRMIYNMTKNVNKEKTIDKVYEIMLKTAIDLIPKATQGSILTMDKE
ENFNYVAILGYDNKLKNIKLNKKEVYLYKINRFKETAIIENPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKL
FGQINIDCTKENYKFKKEDLELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKTLVLID
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFNDLDDKSVISFMENIRQKINKDKIKGINIDF
SYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=36.5384615384615, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081881, Length=158, Percent_Identity=32.9113924050633, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786584, Length=146, Percent_Identity=31.5068493150685, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=27.906976744186, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787262, Length=178, Percent_Identity=30.8988764044944, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787802, Length=154, Percent_Identity=33.1168831168831, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787816, Length=153, Percent_Identity=30.718954248366, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788956, Length=191, Percent_Identity=29.8429319371728, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=30.4093567251462, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=231, Percent_Identity=25.974025974026, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787056, Length=150, Percent_Identity=32, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=27.5167785234899, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=30.379746835443, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42224; Mature: 42224

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKRMIYNMTKNVNKEKTIDKVYEIMLK
CHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TAIDLIPKATQGSILTMDKEENFNYVAILGYDNKLKNIKLNKKEVYLYKINRFKETAIIE
HHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEECCCEEEEEEECCHHHHHHCC
NPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKLFGQINIDCTKENYKFKKEDL
CCHHHHHHHHCHHHHHHHHHHHHCCEEEEEECCEEECCEEEEEEEEEEECCCCCCCHHHH
ELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKTLVLID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFNDLDDKSVISF
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCHHHHHHH
MENIRQKINKDKIKGINIDFSYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV
HHHHHHHHCHHHCCEEEEEHHHCHHHHCCCCCCCHHHHHHHHCCCEEEECCCCEEC
>Mature Secondary Structure
MLYVLIITLFLIMIVYESIIIINLRKNIKKLEYSKRMIYNMTKNVNKEKTIDKVYEIMLK
CHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TAIDLIPKATQGSILTMDKEENFNYVAILGYDNKLKNIKLNKKEVYLYKINRFKETAIIE
HHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEECCCEEEEEEECCHHHHHHCC
NPIKLDEKILNKEKINEFKELEALNISCIISAPIYIDNKLFGQINIDCTKENYKFKKEDL
CCHHHHHHHHCHHHHHHHHHHHHCCEEEEEECCEEECCEEEEEEEEEEECCCCCCCHHHH
ELMDHIKDELQLIIKSFMVKEELIHKANYDELTKIFNRRYFNEIMKNEIKEGNKTLVLID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IDNFKTINDIYGHSTGDFILRVFANLIKRYLGDKDLFFRFGGDEFIVLFNDLDDKSVISF
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCHHHHHHH
MENIRQKINKDKIKGINIDFSYGISKLKNYNMTNYEDIINLADKNMYINKNKKIIV
HHHHHHHHCHHHCCEEEEEHHHCHHHHCCCCCCCHHHHHHHHCCCEEEECCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]