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The map label for this gene is metG [H]

Identifier: 226947300

GI number: 226947300

Start: 99267

End: 101204

Strand: Direct

Name: metG [H]

Synonym: CLM_0119

Alternate gene names: 226947300

Gene position: 99267-101204 (Clockwise)

Preceding gene: 226947299

Following gene: 226947301

Centisome position: 2.39

GC content: 29.98

Gene sequence:

>1938_bases
ATGTCAAAAGGAACTTATTATGTAACAACACCTATATATTATCCGTCAGCGAAACTTCATATAGGAAATACTTATACAAC
GGTTGCTGCAGATACTTTAGCTAGATTTAAAAGACTTACAGGTTATGATGTAATGTTTTTAACTGGTACAGATGAACACG
GACAAAAAATACAAAGATTAGCAGAAGCTAAAAAAGTTACACCTAAGGCTTATGTAGATGAAATAGTAGCGGGAATAAAA
GAACTATGGAAGATGATGAATATAGACTATGATAAATTTATAAGAACAACAGATGAATATCATGTAGAATTAGTGCAAAA
AATATTTAAAACATTGTATGATAAAGGTGATATATATAAAAGCGAGTATTCAGGATGGTATTGTACACCTTGTGAATCAT
TTTGGACAGAAACTCAATTAGAAGATGGAAAGTGCCCAGATTGTGGAAGACCTGTAGAAAAAGCAAAAGAAGAAGCATAT
TTCTTTAAAATGTCAAAATATGCTCCTAAACTTATAGAATATATAGAATCTCATCCAGAGTTTATACAACCAGAATCAAG
AAAAAATGAAATGCTAAATAACTTTTTGAAACCTGGCCTTCAAGATTTATGTGTATCTAGAACATCTTTTGATTGGGGAA
TACCAGTTACTTTTGATGAAAAACATGTAATTTATGTTTGGATAGATGCACTTGCTAATTATATAACAGCATTAGGATAT
AATACAGAAAAGGATGAATTATATAAAAAATACTGGCCAGCAGATGTACACTTAGTTGGTAAGGATATATTAAGATTTCA
TACTATATATTGGCCAATTATGCTTATGGCATTAGATATACCTCTTCCAAAGCAGGTATTTGGTCATGGATGGTTATTGG
TAGATGGGGGGAAAATGTCAAAATCCAAAGGTAATGTGGTAGATCCAGTAGTTTTAGTAAATCATTTTGGAACAGATCCT
GTAAGATACTATCTTCTTCGAGAAATACCTTTTGGCGCAGACGGAATATTTAATAATGAGATTTTTATAAAGAAAATAAA
TTCAGATTTAGCAAATGATTTGGGAAATTTATTATCTAGAACAGTAGCTATGATAGAAAAATATTTTGATGGAGAAATAC
CAGCAGGAAATTGCAAAGAGGATATAGATGAAGAGTTAATAAAATTAGCCCTTGAAACTCCAAAAAAAGTGGAAGAAAAT
ATGGAAAAACTAAGAATACCAGAAGCTTTAGAAGAAATATGGACATTTATAGGAAGAGCTAATAAGTATATAGATGAAAC
AACACCATGGATTCTTGCAAAAGATGAGTCTAAAAAAGAGAGATTGGGTACAGTTTTATATAATCTAGCAGAAAGTTTAA
GAATAATATCAGTATGTATATCAGCATTCATACCAGAAACAAGTATAAAAATAAATGAACAAATAAATGCGGATGTTTTA
AATTGGGATAGTTTAAGTAAATTTAATGGAACTAAAGTAGGCACAAAGGTTAAAAAAGGAGAAGCAATATTCCCAAGAAT
AGATGTAGAAAAGAAAATAGAAGAGCTAAATAAACTTAAAGAAGAACAATTAAAACAAAACAAAGCTAAAAATATAGCAC
CTATAAAAGAAGAAATAACTATAGATGATTTTGAAAAAATAGATTTAAGGGTAGCTAAGGTTTTAGAATGTGAACCTATA
AAAGGAGCTAAAAAATTATTAAAACTAAAAGTTAGTTTAGGTGGAGAAGAAAGACAGGTAGTATCAGGAATAGCTAAATA
CTATAAACCAGAAGACTTAATAGGTAAAAAAGTAGTATTAGCAGCTAATTTAAAGCCTGTTAAGCTAAGAGGAGAATTGT
CACAAGGAATGATTTTAGCAGCATCAACAGAAGATGATAGTGAGCTATTTGTAGCATCAATAGATGGAGACATTGAAGAA
GGAAATATAGTAAGGTAA

Upstream 100 bases:

>100_bases
AAGGTGAATTAGAGTGGTACAGCGAAATTTACGCCTCTATATAAAACTAAAGGTTTTATATAGGGGTTTTTTATATTATA
AAAATGAAGGAGGACTAATA

Downstream 100 bases:

>100_bases
AAATTAAAGCTAATTATATCAAATTAAGAACTAAGCTGTTGACAAATAAAGTTTGTTAGCAGCTTTGTTTTTGGTACATA
ATATATTGAAAGTAGTAAAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MSKGTYYVTTPIYYPSAKLHIGNTYTTVAADTLARFKRLTGYDVMFLTGTDEHGQKIQRLAEAKKVTPKAYVDEIVAGIK
ELWKMMNIDYDKFIRTTDEYHVELVQKIFKTLYDKGDIYKSEYSGWYCTPCESFWTETQLEDGKCPDCGRPVEKAKEEAY
FFKMSKYAPKLIEYIESHPEFIQPESRKNEMLNNFLKPGLQDLCVSRTSFDWGIPVTFDEKHVIYVWIDALANYITALGY
NTEKDELYKKYWPADVHLVGKDILRFHTIYWPIMLMALDIPLPKQVFGHGWLLVDGGKMSKSKGNVVDPVVLVNHFGTDP
VRYYLLREIPFGADGIFNNEIFIKKINSDLANDLGNLLSRTVAMIEKYFDGEIPAGNCKEDIDEELIKLALETPKKVEEN
MEKLRIPEALEEIWTFIGRANKYIDETTPWILAKDESKKERLGTVLYNLAESLRIISVCISAFIPETSIKINEQINADVL
NWDSLSKFNGTKVGTKVKKGEAIFPRIDVEKKIEELNKLKEEQLKQNKAKNIAPIKEEITIDDFEKIDLRVAKVLECEPI
KGAKKLLKLKVSLGGEERQVVSGIAKYYKPEDLIGKKVVLAANLKPVKLRGELSQGMILAASTEDDSELFVASIDGDIEE
GNIVR

Sequences:

>Translated_645_residues
MSKGTYYVTTPIYYPSAKLHIGNTYTTVAADTLARFKRLTGYDVMFLTGTDEHGQKIQRLAEAKKVTPKAYVDEIVAGIK
ELWKMMNIDYDKFIRTTDEYHVELVQKIFKTLYDKGDIYKSEYSGWYCTPCESFWTETQLEDGKCPDCGRPVEKAKEEAY
FFKMSKYAPKLIEYIESHPEFIQPESRKNEMLNNFLKPGLQDLCVSRTSFDWGIPVTFDEKHVIYVWIDALANYITALGY
NTEKDELYKKYWPADVHLVGKDILRFHTIYWPIMLMALDIPLPKQVFGHGWLLVDGGKMSKSKGNVVDPVVLVNHFGTDP
VRYYLLREIPFGADGIFNNEIFIKKINSDLANDLGNLLSRTVAMIEKYFDGEIPAGNCKEDIDEELIKLALETPKKVEEN
MEKLRIPEALEEIWTFIGRANKYIDETTPWILAKDESKKERLGTVLYNLAESLRIISVCISAFIPETSIKINEQINADVL
NWDSLSKFNGTKVGTKVKKGEAIFPRIDVEKKIEELNKLKEEQLKQNKAKNIAPIKEEITIDDFEKIDLRVAKVLECEPI
KGAKKLLKLKVSLGGEERQVVSGIAKYYKPEDLIGKKVVLAANLKPVKLRGELSQGMILAASTEDDSELFVASIDGDIEE
GNIVR
>Mature_644_residues
SKGTYYVTTPIYYPSAKLHIGNTYTTVAADTLARFKRLTGYDVMFLTGTDEHGQKIQRLAEAKKVTPKAYVDEIVAGIKE
LWKMMNIDYDKFIRTTDEYHVELVQKIFKTLYDKGDIYKSEYSGWYCTPCESFWTETQLEDGKCPDCGRPVEKAKEEAYF
FKMSKYAPKLIEYIESHPEFIQPESRKNEMLNNFLKPGLQDLCVSRTSFDWGIPVTFDEKHVIYVWIDALANYITALGYN
TEKDELYKKYWPADVHLVGKDILRFHTIYWPIMLMALDIPLPKQVFGHGWLLVDGGKMSKSKGNVVDPVVLVNHFGTDPV
RYYLLREIPFGADGIFNNEIFIKKINSDLANDLGNLLSRTVAMIEKYFDGEIPAGNCKEDIDEELIKLALETPKKVEENM
EKLRIPEALEEIWTFIGRANKYIDETTPWILAKDESKKERLGTVLYNLAESLRIISVCISAFIPETSIKINEQINADVLN
WDSLSKFNGTKVGTKVKKGEAIFPRIDVEKKIEELNKLKEEQLKQNKAKNIAPIKEEITIDDFEKIDLRVAKVLECEPIK
GAKKLLKLKVSLGGEERQVVSGIAKYYKPEDLIGKKVVLAANLKPVKLRGELSQGMILAASTEDDSELFVASIDGDIEEG
NIVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=547, Percent_Identity=33.27239488117, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI14043022, Length=511, Percent_Identity=27.2015655577299, Blast_Score=175, Evalue=1e-43,
Organism=Escherichia coli, GI1788432, Length=683, Percent_Identity=29.4289897510981, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1786861, Length=174, Percent_Identity=29.8850574712644, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789455, Length=92, Percent_Identity=39.1304347826087, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI212645096, Length=485, Percent_Identity=35.0515463917526, Blast_Score=274, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI71992781, Length=372, Percent_Identity=33.6021505376344, Blast_Score=189, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17540794, Length=575, Percent_Identity=26.2608695652174, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71997510, Length=180, Percent_Identity=28.3333333333333, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71997517, Length=180, Percent_Identity=28.3333333333333, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321610, Length=520, Percent_Identity=32.8846153846154, Blast_Score=274, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6321704, Length=561, Percent_Identity=26.2032085561497, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6323414, Length=178, Percent_Identity=28.6516853932584, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21358483, Length=512, Percent_Identity=33.0078125, Blast_Score=293, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24655311, Length=527, Percent_Identity=26.5654648956357, Blast_Score=177, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21355409, Length=178, Percent_Identity=26.9662921348315, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 73657; Mature: 73525

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGTYYVTTPIYYPSAKLHIGNTYTTVAADTLARFKRLTGYDVMFLTGTDEHGQKIQRL
CCCCCEEEECCEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
AEAKKVTPKAYVDEIVAGIKELWKMMNIDYDKFIRTTDEYHVELVQKIFKTLYDKGDIYK
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCH
SEYSGWYCTPCESFWTETQLEDGKCPDCGRPVEKAKEEAYFFKMSKYAPKLIEYIESHPE
HCCCCEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FIQPESRKNEMLNNFLKPGLQDLCVSRTSFDWGIPVTFDEKHVIYVWIDALANYITALGY
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEECCCEEEEEEHHHHHHHHHHHCC
NTEKDELYKKYWPADVHLVGKDILRFHTIYWPIMLMALDIPLPKQVFGHGWLLVDGGKMS
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCC
KSKGNVVDPVVLVNHFGTDPVRYYLLREIPFGADGIFNNEIFIKKINSDLANDLGNLLSR
CCCCCCCCHHHEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
TVAMIEKYFDGEIPAGNCKEDIDEELIKLALETPKKVEENMEKLRIPEALEEIWTFIGRA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
NKYIDETTPWILAKDESKKERLGTVLYNLAESLRIISVCISAFIPETSIKINEQINADVL
HHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEE
NWDSLSKFNGTKVGTKVKKGEAIFPRIDVEKKIEELNKLKEEQLKQNKAKNIAPIKEEIT
CCCCCCCCCCCCCCCEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
IDDFEKIDLRVAKVLECEPIKGAKKLLKLKVSLGGEERQVVSGIAKYYKPEDLIGKKVVL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEE
AANLKPVKLRGELSQGMILAASTEDDSELFVASIDGDIEEGNIVR
EECCCCEEEECCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SKGTYYVTTPIYYPSAKLHIGNTYTTVAADTLARFKRLTGYDVMFLTGTDEHGQKIQRL
CCCCEEEECCEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
AEAKKVTPKAYVDEIVAGIKELWKMMNIDYDKFIRTTDEYHVELVQKIFKTLYDKGDIYK
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCH
SEYSGWYCTPCESFWTETQLEDGKCPDCGRPVEKAKEEAYFFKMSKYAPKLIEYIESHPE
HCCCCEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FIQPESRKNEMLNNFLKPGLQDLCVSRTSFDWGIPVTFDEKHVIYVWIDALANYITALGY
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEECCCEEEEEEHHHHHHHHHHHCC
NTEKDELYKKYWPADVHLVGKDILRFHTIYWPIMLMALDIPLPKQVFGHGWLLVDGGKMS
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCC
KSKGNVVDPVVLVNHFGTDPVRYYLLREIPFGADGIFNNEIFIKKINSDLANDLGNLLSR
CCCCCCCCHHHEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
TVAMIEKYFDGEIPAGNCKEDIDEELIKLALETPKKVEENMEKLRIPEALEEIWTFIGRA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
NKYIDETTPWILAKDESKKERLGTVLYNLAESLRIISVCISAFIPETSIKINEQINADVL
HHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEE
NWDSLSKFNGTKVGTKVKKGEAIFPRIDVEKKIEELNKLKEEQLKQNKAKNIAPIKEEIT
CCCCCCCCCCCCCCCEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
IDDFEKIDLRVAKVLECEPIKGAKKLLKLKVSLGGEERQVVSGIAKYYKPEDLIGKKVVL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEE
AANLKPVKLRGELSQGMILAASTEDDSELFVASIDGDIEEGNIVR
EECCCCEEEECCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]