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The map label for this gene is cobB

Identifier: 226225290

GI number: 226225290

Start: 2785393

End: 2786082

Strand: Reverse

Name: cobB

Synonym: Lm4b_02713

Alternate gene names: 226225290

Gene position: 2786082-2785393 (Counterclockwise)

Preceding gene: 226225291

Following gene: 226225289

Centisome position: 95.65

GC content: 40.43

Gene sequence:

>690_bases
ATGAATAAGTTGAATGAAGCACTTAAAAAAGCTGAACACATCGTTTTTCTTACCGGGGCGGGTGTTTCAGTGCCTTCTGG
AATTCCGGATTATCGCTCGAAAAACGGCTTATATGCTGGGATGAGTAGCCCGGAATATATGTTGAGCCATACGTGTCTTG
TGCGTGAACCGGAGAAGTTTTATCAATTTGTTACAGAAAACATGTACTATCCGGATGCAGAGCCAAATGCGATTCATACA
AAAATGGCTGAAATCGAAGCGGAAAAAGATGTCACGATTATTACGCAAAATATTGATGGTTTGCATGAAAAAGCAGGTTC
AAAAAAAGTCGTAAATTTTCACGGTAGTTTATATCATTGTTATTGCCAAAAATGCGGAATGTCTGTTACCGCTGAGGAGT
ACTTGAAATCGGATATCCATTCGGGTTGTGGTGGCGTCATTCGTCCGGATGTGGTGCTTTATGAAGAAGCGATTCCAGAG
AGTGCGATAGACCAATCTCTTGCAGCTATAAGACAAGCGGATTTGATAGTGATAGTAGGGACATCTTTCCGCGTGAGTCC
GTTTTGTAATTTAACAGATTATCGAAATAAAAAAGCGCGGATTTTTGCTGTAAATAAAGAACAGATTTCGCTTCCTTATC
CATTTGAAATGATGGAAAGTGACGCTGTGAAAGTTTTCGCGGAGATTTGA

Upstream 100 bases:

>100_bases
GTCTATGTTGCGGAAACGGATGAAGCGGAGCAAGATGTTGATTTGCGAAAATGGTGGAACTGGAAAGATAAACAACATAC
AAATGAAAGGAATTTAAAGA

Downstream 100 bases:

>100_bases
AACCTTGCAGTAATAGGGGTGGCGTGATAAAATTTTCTTAACCGGCAAATTGTCCGGAATATTTAGAGAAAATGCATGGT
CGTTTTCTTGATGAAAATAA

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNKLNEALKKAEHIVFLTGAGVSVPSGIPDYRSKNGLYAGMSSPEYMLSHTCLVREPEKFYQFVTENMYYPDAEPNAIHT
KMAEIEAEKDVTIITQNIDGLHEKAGSKKVVNFHGSLYHCYCQKCGMSVTAEEYLKSDIHSGCGGVIRPDVVLYEEAIPE
SAIDQSLAAIRQADLIVIVGTSFRVSPFCNLTDYRNKKARIFAVNKEQISLPYPFEMMESDAVKVFAEI

Sequences:

>Translated_229_residues
MNKLNEALKKAEHIVFLTGAGVSVPSGIPDYRSKNGLYAGMSSPEYMLSHTCLVREPEKFYQFVTENMYYPDAEPNAIHT
KMAEIEAEKDVTIITQNIDGLHEKAGSKKVVNFHGSLYHCYCQKCGMSVTAEEYLKSDIHSGCGGVIRPDVVLYEEAIPE
SAIDQSLAAIRQADLIVIVGTSFRVSPFCNLTDYRNKKARIFAVNKEQISLPYPFEMMESDAVKVFAEI
>Mature_229_residues
MNKLNEALKKAEHIVFLTGAGVSVPSGIPDYRSKNGLYAGMSSPEYMLSHTCLVREPEKFYQFVTENMYYPDAEPNAIHT
KMAEIEAEKDVTIITQNIDGLHEKAGSKKVVNFHGSLYHCYCQKCGMSVTAEEYLKSDIHSGCGGVIRPDVVLYEEAIPE
SAIDQSLAAIRQADLIVIVGTSFRVSPFCNLTDYRNKKARIFAVNKEQISLPYPFEMMESDAVKVFAEI

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI300795542, Length=205, Percent_Identity=35.609756097561, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI13787215, Length=223, Percent_Identity=32.7354260089686, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI6912664, Length=223, Percent_Identity=32.7354260089686, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI6912660, Length=218, Percent_Identity=28.4403669724771, Blast_Score=100, Evalue=8e-22,
Organism=Homo sapiens, GI63054862, Length=210, Percent_Identity=29.0476190476191, Blast_Score=98, Evalue=5e-21,
Organism=Homo sapiens, GI7657575, Length=200, Percent_Identity=30, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI6912662, Length=224, Percent_Identity=26.7857142857143, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI13775602, Length=228, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=8e-18,
Organism=Homo sapiens, GI13775600, Length=228, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI300797705, Length=197, Percent_Identity=27.9187817258883, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI308199517, Length=187, Percent_Identity=31.5508021390374, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17541892, Length=222, Percent_Identity=33.7837837837838, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71990482, Length=252, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17567771, Length=252, Percent_Identity=26.1904761904762, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71990487, Length=254, Percent_Identity=28.3464566929134, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505510, Length=227, Percent_Identity=24.2290748898678, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325242, Length=231, Percent_Identity=30.3030303030303, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320163, Length=242, Percent_Identity=26.8595041322314, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324504, Length=253, Percent_Identity=24.901185770751, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17137536, Length=263, Percent_Identity=29.277566539924, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28571445, Length=213, Percent_Identity=28.6384976525822, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648389, Length=217, Percent_Identity=28.110599078341, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24645650, Length=208, Percent_Identity=27.4038461538462, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_LISMF (Q71W25)

Other databases:

- EMBL:   AE017262
- RefSeq:   YP_015314.1
- ProteinModelPortal:   Q71W25
- SMR:   Q71W25
- STRING:   Q71W25
- GeneID:   2797217
- GenomeReviews:   AE017262_GR
- KEGG:   lmf:LMOf2365_2726
- TIGR:   LMOf2365_2726
- eggNOG:   COG0846
- HOGENOM:   HBG641281
- OMA:   AGLINYF
- ProtClustDB:   PRK00481
- BioCyc:   LMON265669:LMOF2365_2726-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 25560; Mature: 25560

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLNEALKKAEHIVFLTGAGVSVPSGIPDYRSKNGLYAGMSSPEYMLSHTCLVREPEKF
CCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHCCCEECCHHHH
YQFVTENMYYPDAEPNAIHTKMAEIEAEKDVTIITQNIDGLHEKAGSKKVVNFHGSLYHC
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCEEEEECCCHHHH
YCQKCGMSVTAEEYLKSDIHSGCGGVIRPDVVLYEEAIPESAIDQSLAAIRQADLIVIVG
HHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHCCEEEEEC
TSFRVSPFCNLTDYRNKKARIFAVNKEQISLPYPFEMMESDAVKVFAEI
CCEEECCCCCCCCCCCCCEEEEEECCCEEECCCCHHHHHCCHHEEEECC
>Mature Secondary Structure
MNKLNEALKKAEHIVFLTGAGVSVPSGIPDYRSKNGLYAGMSSPEYMLSHTCLVREPEKF
CCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHCCCEECCHHHH
YQFVTENMYYPDAEPNAIHTKMAEIEAEKDVTIITQNIDGLHEKAGSKKVVNFHGSLYHC
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCEEEEECCCHHHH
YCQKCGMSVTAEEYLKSDIHSGCGGVIRPDVVLYEEAIPESAIDQSLAAIRQADLIVIVG
HHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHCCEEEEEC
TSFRVSPFCNLTDYRNKKARIFAVNKEQISLPYPFEMMESDAVKVFAEI
CCEEECCCCCCCCCCCCCEEEEEECCCEEECCCCHHHHHCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA