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The map label for this gene is dhaK [H]

Identifier: 226225246

GI number: 226225246

Start: 2741770

End: 2742759

Strand: Direct

Name: dhaK [H]

Synonym: Lm4b_02668

Alternate gene names: 226225246

Gene position: 2741770-2742759 (Clockwise)

Preceding gene: 226225242

Following gene: 226225247

Centisome position: 94.13

GC content: 44.14

Gene sequence:

>990_bases
ATGAAGAAGATTCTTAACGGTACTGATCAAGTAGTAGAACAGATGGTGGAAGGTTTAGTGAAATCGCATGCTGATGTTGT
TCACCGTGTCGAAGGAACTCGCGTCATTGCAAGAAATGATAAACGTCCAGGAAAAGTCGGACTTGTAAGCGGTGGAGGTT
CTGGTCACGAGCCGGCTCATGCTGGTTATGTAGGTCGCGGAATGCTCTCTGCGGCTGTATGTGGCGATGTTTTCACTTCC
CCAACTCCCGACCAAATTTATGAAGGTATAAAAGCCGCAGATCAAGGCGCTGGTGTACTTTTAATCGTAAAAAATTATAC
TGGTGACGTAATGAATTTTGAAATGGCGGCAGATTTAGCAGATGCCGATGATATTAAAGTAGAACAAATTGTAGTAGATG
ATGATATTGCTGTTGAGGATAGTACTTTTACAACGGGACGCCGCGGTGTAGCCGGGACTGTTCTCGTGCATAAAATTATC
GGGGCAGCCGCAGAAGCAGGCGCATCTCTTGATGAACTGAAAGCCCTTGGTGAAAAAGTAATTGCTTCGGTTAAAACGCT
TGGTGTCGCACTTACTCCATGTACTGTTCCTGAGGTTGGACATCCTGGCTTTGAGCTTGGTGACGACGAAATCGAACTTG
GCATTGGTATCCACGGTGAACCTGGCTTTACGCGTGAAAAAATCATGCCATCTGCAAGTTTAGCGAAGCAACTGTACGAA
CGGATTAGCAATGAAAGTAAACTCCTACCTGGAGATAAAGTGGTTGTGCTCGTGAACGGCATGGGCGCAACACCACTTAT
GGAACAATATGTTTTCGCAAATGATGTCCATGAACTTCTAAAAAATGCCGGCGTTCAAGTTGAAAAAACACTCGTTGGAG
ATTATATGACTTCTCTTGAAATGGCTGGTTTATCCTTAACCATTTTGAAATTAGAGGACGAAAAATGGGTTGATATGTTG
AAACTCCCAGTGGACACAATTGCATGGTAA

Upstream 100 bases:

>100_bases
CATTTTATAAGTTGTATAATAAAGACAGCAACTACGTTTTGACTTTCTAGTAAAGGGAAATTGAGGTAAGAGGACTATTT
TAAAGGAGGTTTTTTGTCAG

Downstream 100 bases:

>100_bases
TAAAATTTGGAGGAATGAATCATGACTTATGATAAAGATTGGGCGTTACGCTGGTTAAATGACTTCGGCGAACGCGTACA
AGAAAACAAACAATTATTAA

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKILNGTDQVVEQMVEGLVKSHADVVHRVEGTRVIARNDKRPGKVGLVSGGGSGHEPAHAGYVGRGMLSAAVCGDVFTS
PTPDQIYEGIKAADQGAGVLLIVKNYTGDVMNFEMAADLADADDIKVEQIVVDDDIAVEDSTFTTGRRGVAGTVLVHKII
GAAAEAGASLDELKALGEKVIASVKTLGVALTPCTVPEVGHPGFELGDDEIELGIGIHGEPGFTREKIMPSASLAKQLYE
RISNESKLLPGDKVVVLVNGMGATPLMEQYVFANDVHELLKNAGVQVEKTLVGDYMTSLEMAGLSLTILKLEDEKWVDML
KLPVDTIAW

Sequences:

>Translated_329_residues
MKKILNGTDQVVEQMVEGLVKSHADVVHRVEGTRVIARNDKRPGKVGLVSGGGSGHEPAHAGYVGRGMLSAAVCGDVFTS
PTPDQIYEGIKAADQGAGVLLIVKNYTGDVMNFEMAADLADADDIKVEQIVVDDDIAVEDSTFTTGRRGVAGTVLVHKII
GAAAEAGASLDELKALGEKVIASVKTLGVALTPCTVPEVGHPGFELGDDEIELGIGIHGEPGFTREKIMPSASLAKQLYE
RISNESKLLPGDKVVVLVNGMGATPLMEQYVFANDVHELLKNAGVQVEKTLVGDYMTSLEMAGLSLTILKLEDEKWVDML
KLPVDTIAW
>Mature_329_residues
MKKILNGTDQVVEQMVEGLVKSHADVVHRVEGTRVIARNDKRPGKVGLVSGGGSGHEPAHAGYVGRGMLSAAVCGDVFTS
PTPDQIYEGIKAADQGAGVLLIVKNYTGDVMNFEMAADLADADDIKVEQIVVDDDIAVEDSTFTTGRRGVAGTVLVHKII
GAAAEAGASLDELKALGEKVIASVKTLGVALTPCTVPEVGHPGFELGDDEIELGIGIHGEPGFTREKIMPSASLAKQLYE
RISNESKLLPGDKVVVLVNGMGATPLMEQYVFANDVHELLKNAGVQVEKTLVGDYMTSLEMAGLSLTILKLEDEKWVDML
KLPVDTIAW

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone [H]

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=341, Percent_Identity=41.3489736070381, Blast_Score=242, Evalue=4e-64,
Organism=Escherichia coli, GI87081857, Length=352, Percent_Identity=44.3181818181818, Blast_Score=293, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17565018, Length=329, Percent_Identity=37.3860182370821, Blast_Score=217, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6321055, Length=338, Percent_Identity=38.1656804733728, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323570, Length=280, Percent_Identity=39.6428571428571, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 34883; Mature: 34883

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILNGTDQVVEQMVEGLVKSHADVVHRVEGTRVIARNDKRPGKVGLVSGGGSGHEPAH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCC
AGYVGRGMLSAAVCGDVFTSPTPDQIYEGIKAADQGAGVLLIVKNYTGDVMNFEMAADLA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEHHHHHCCC
DADDIKVEQIVVDDDIAVEDSTFTTGRRGVAGTVLVHKIIGAAAEAGASLDELKALGEKV
CCCCCEEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IASVKTLGVALTPCTVPEVGHPGFELGDDEIELGIGIHGEPGFTREKIMPSASLAKQLYE
HHHHHHHCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHCCCCHHHHHHHHH
RISNESKLLPGDKVVVLVNGMGATPLMEQYVFANDVHELLKNAGVQVEKTLVGDYMTSLE
HHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
MAGLSLTILKLEDEKWVDMLKLPVDTIAW
HCCCEEEEEEECCHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MKKILNGTDQVVEQMVEGLVKSHADVVHRVEGTRVIARNDKRPGKVGLVSGGGSGHEPAH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCC
AGYVGRGMLSAAVCGDVFTSPTPDQIYEGIKAADQGAGVLLIVKNYTGDVMNFEMAADLA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEHHHHHCCC
DADDIKVEQIVVDDDIAVEDSTFTTGRRGVAGTVLVHKIIGAAAEAGASLDELKALGEKV
CCCCCEEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IASVKTLGVALTPCTVPEVGHPGFELGDDEIELGIGIHGEPGFTREKIMPSASLAKQLYE
HHHHHHHCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHCCCCHHHHHHHHH
RISNESKLLPGDKVVVLVNGMGATPLMEQYVFANDVHELLKNAGVQVEKTLVGDYMTSLE
HHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
MAGLSLTILKLEDEKWVDMLKLPVDTIAW
HCCCEEEEEEECCHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]