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The map label for this gene is php [H]

Identifier: 226225192

GI number: 226225192

Start: 2686196

End: 2687188

Strand: Reverse

Name: php [H]

Synonym: Lm4b_02614

Alternate gene names: 226225192

Gene position: 2687188-2686196 (Counterclockwise)

Preceding gene: 226225193

Following gene: 226225191

Centisome position: 92.26

GC content: 39.88

Gene sequence:

>993_bases
ATGAGTTTTATTCGTACTTTTTATGGAGATATTGCCCCTGAACAACTGGGCTTCACTTATTCACACGAGCACATCGTTTG
TGTACCAGCTTACTGGCAAGAGCGAGATGCCGATGATTTACTTTTGGATGATAAAGAAAAATCACAACTTGATGTACAAG
ATTTCGCCGATTTAGGAGGCAAAACAATTGTCGATGCAACCGCGGTTGATTACGGCAGACGCGTCTTAGATGTGGCGCAA
ATTTCCAAAGAAACAGGCATCCAAATCGTTGGAACAGCTGGCTTTAACAAAAGTTTCTTATGGGACGGGAAGATTAAACC
AGAACTGAAACCAATCATCGGGGATTTTGAAACCTACTATGAATGGATTGAAAATACTACTACAGACAAACTAACGGAAT
TCGTTGTAAATGAAGTCGAAAATGGGCTTGAAGGAACGCCGTATAAGGCTGGTCAGGTGAAATTTGGCACTGGCTACAAT
ATGATTACACCTTTAGAAGAAAAGACAATACGTGCGGTTGCAAGAGCACATCACGAGACAAAAGCACCAATTCATTCTCA
CACAGAAGCGGGAACGATGGCTTTAGAACAAATCGAGATTTTAAAACAAGAAAATATCCCGTTAGAATACCTTTCTATTG
GTCATATGGACCGCAACCTAGATCCGTATTATCACAAGCAAGTTGCCAAAACAGGCGCATTCATGTCATTTGATGGCATC
GCAAAAATCAAATACGCACCAGAAAGCGCTCGGATTGCAGCGATTCTATATCTAGTTTCGGAAGGCTTTGAAGATCAAAT
TTTAGTTAGTGGTGATACAGCACGCAAAACTTATTATAAACATTATGGTCACGGACCAGGACTTGAATATATCGCCAAAA
AATGGGTGCCACGCTTTATTGATGAAGCAAACGAAAAAGGCTTTGACGGAGAAAAATTAGTTAAAAAATTCTTCGTGGAT
AACCCAGCAAGATGTTTTACTTTTAAAAAATAG

Upstream 100 bases:

>100_bases
TGTCAGCATGTTAGCGAAGTTATTCTTTTAAGTAAAATCTAAACCTCTTCCTTGTCTAGCATTCAAGAGGAAGAGGTTTT
TTGAAAGGAAGTTTTAAAAA

Downstream 100 bases:

>100_bases
GAGGCGAAATCTGTGTTATATGCAGATGAAAATTCATTTGATATTGGCGCGAAAATCACGAAAACAAAACCAGTCCTTTT
GCCAATAGGAGCAGTCGAAG

Product: Phosphotriesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSFIRTFYGDIAPEQLGFTYSHEHIVCVPAYWQERDADDLLLDDKEKSQLDVQDFADLGGKTIVDATAVDYGRRVLDVAQ
ISKETGIQIVGTAGFNKSFLWDGKIKPELKPIIGDFETYYEWIENTTTDKLTEFVVNEVENGLEGTPYKAGQVKFGTGYN
MITPLEEKTIRAVARAHHETKAPIHSHTEAGTMALEQIEILKQENIPLEYLSIGHMDRNLDPYYHKQVAKTGAFMSFDGI
AKIKYAPESARIAAILYLVSEGFEDQILVSGDTARKTYYKHYGHGPGLEYIAKKWVPRFIDEANEKGFDGEKLVKKFFVD
NPARCFTFKK

Sequences:

>Translated_330_residues
MSFIRTFYGDIAPEQLGFTYSHEHIVCVPAYWQERDADDLLLDDKEKSQLDVQDFADLGGKTIVDATAVDYGRRVLDVAQ
ISKETGIQIVGTAGFNKSFLWDGKIKPELKPIIGDFETYYEWIENTTTDKLTEFVVNEVENGLEGTPYKAGQVKFGTGYN
MITPLEEKTIRAVARAHHETKAPIHSHTEAGTMALEQIEILKQENIPLEYLSIGHMDRNLDPYYHKQVAKTGAFMSFDGI
AKIKYAPESARIAAILYLVSEGFEDQILVSGDTARKTYYKHYGHGPGLEYIAKKWVPRFIDEANEKGFDGEKLVKKFFVD
NPARCFTFKK
>Mature_329_residues
SFIRTFYGDIAPEQLGFTYSHEHIVCVPAYWQERDADDLLLDDKEKSQLDVQDFADLGGKTIVDATAVDYGRRVLDVAQI
SKETGIQIVGTAGFNKSFLWDGKIKPELKPIIGDFETYYEWIENTTTDKLTEFVVNEVENGLEGTPYKAGQVKFGTGYNM
ITPLEEKTIRAVARAHHETKAPIHSHTEAGTMALEQIEILKQENIPLEYLSIGHMDRNLDPYYHKQVAKTGAFMSFDGIA
KIKYAPESARIAAILYLVSEGFEDQILVSGDTARKTYYKHYGHGPGLEYIAKKWVPRFIDEANEKGFDGEKLVKKFFVDN
PARCFTFKK

Specific function: Its real enzymatic activity is not yet known. It was tested for general esterase, aminopeptidase, sulfatase, phosphatase, carbonic anhydrase, phosphodiesterase, and phosphotriesterase activities with the following substrates:P- nitrophenyl acetate, L-alan

COG id: COG1735

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphotriesterase family [H]

Homologues:

Organism=Homo sapiens, GI20070186, Length=367, Percent_Identity=25.0681198910082, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI47933341, Length=367, Percent_Identity=25.0681198910082, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1789780, Length=313, Percent_Identity=28.4345047923323, Blast_Score=131, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017947
- InterPro:   IPR001559 [H]

Pfam domain/function: PF02126 PTE [H]

EC number: NA

Molecular weight: Translated: 37404; Mature: 37272

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIRTFYGDIAPEQLGFTYSHEHIVCVPAYWQERDADDLLLDDKEKSQLDVQDFADLGG
CCHHHHHHCCCCHHHCCCEECCCEEEEEECCCCCCCCCCCEECCCCCCCCCHHHHHHCCC
KTIVDATAVDYGRRVLDVAQISKETGIQIVGTAGFNKSFLWDGKIKPELKPIIGDFETYY
CEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCCCHHHHHCCHHHHH
EWIENTTTDKLTEFVVNEVENGLEGTPYKAGQVKFGTGYNMITPLEEKTIRAVARAHHET
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KAPIHSHTEAGTMALEQIEILKQENIPLEYLSIGHMDRNLDPYYHKQVAKTGAFMSFDGI
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEECCCC
AKIKYAPESARIAAILYLVSEGFEDQILVSGDTARKTYYKHYGHGPGLEYIAKKWVPRFI
EEEEECCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DEANEKGFDGEKLVKKFFVDNPARCFTFKK
HHHCCCCCCHHHHHHHHHCCCCHHEEEECC
>Mature Secondary Structure 
SFIRTFYGDIAPEQLGFTYSHEHIVCVPAYWQERDADDLLLDDKEKSQLDVQDFADLGG
CHHHHHHCCCCHHHCCCEECCCEEEEEECCCCCCCCCCCEECCCCCCCCCHHHHHHCCC
KTIVDATAVDYGRRVLDVAQISKETGIQIVGTAGFNKSFLWDGKIKPELKPIIGDFETYY
CEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCCCHHHHHCCHHHHH
EWIENTTTDKLTEFVVNEVENGLEGTPYKAGQVKFGTGYNMITPLEEKTIRAVARAHHET
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KAPIHSHTEAGTMALEQIEILKQENIPLEYLSIGHMDRNLDPYYHKQVAKTGAFMSFDGI
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEECCCC
AKIKYAPESARIAAILYLVSEGFEDQILVSGDTARKTYYKHYGHGPGLEYIAKKWVPRFI
EEEEECCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DEANEKGFDGEKLVKKFFVDNPARCFTFKK
HHHCCCCCCHHHHHHHHHCCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9548740 [H]