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The map label for this gene is apbE [H]

Identifier: 226225181

GI number: 226225181

Start: 2674785

End: 2675867

Strand: Reverse

Name: apbE [H]

Synonym: Lm4b_02603

Alternate gene names: 226225181

Gene position: 2675867-2674785 (Counterclockwise)

Preceding gene: 226225182

Following gene: 226225180

Centisome position: 91.87

GC content: 36.93

Gene sequence:

>1083_bases
ATGAAGAAATGGAAAATAATAATTTCAGTTATCATATTGGCGCTTGTTGTATCAGCGTGTGGAAATTCTAGTAAAGAAAC
AAAGAGTGAACCAAGTGATTCAAAAAAACTAATGGATCAACCATACTCCAAAACAGACTTTCTAATGGGCACAGTAGTAA
CGCTTAAAATTTATGATAAAGGCAAAGAAGACGTGCTTGATAAAGGCTTCGATCGAATCAAAGATCTAGCTGCCAAGATT
ACCACAAGTGATTCGGAAAAAACGTCTGAAGTGGATAAAATCAACGAACAAGCCGGCAAAAAACCAGTGAAAGTATCTAA
AGATGTATACTACTTAATTCAAGAAGGGCTTAAATACTCCGAGAACTCTGGTGGTAGCTTCGATATTACTATTGGGCCGT
TAACATCTCTATGGCACATCGGTTTTTCTGATGCGCGCAAACCTTCTCAAGCAGAAATTGACGCGGTGTTACCATTAATT
AATTACAAAGACGTTAAAATGAATGATAAAGACCAAACAGTATACTTGGAAAAAGAAGGTATGGAACTAGATTTAGGGGC
AATTGCTAAAGGCTTCATTACTGACGAGACTTTAAAGGTATTCAAAGAAAATAAAGTAACAACCTCTATCATAGATTTAG
GTGGAAACATTTATGTTCAAGGCAACAACCCGAATGGCAACAAATGGAATGTCGGAATTCAAGATCCGTTTTCTCCGCGT
GGAAGTGTTATTGGGAAACTTCCTGAATCTAATATGTCTATCGTAACTTCTGGTATTTATGAACGTTACCTTGAAGTGGA
TGGTAAAACATATCATCACATCTTAGACCCGAAAACAGGATATCCGTTTGACAATGATATCGCTGGAGTTTCGATTGTAT
CTAAAAAATCAATTGATGGCGACGGTCTATCCACCGCTACTTTCTCCAAAGGGGTTAAAGGTGGCATGGACTACATTGAA
CAATTCGAAGGTGTAGATGCTATTTTCATCAGTAAAGAGAAAAAAGTATACGAAACTTCTGGCTTAAAAGGTCAATTCGA
ATTAACGGATAAAGACTTCCAGATGGACACGCTAAAAAAATAA

Upstream 100 bases:

>100_bases
AAAAACAGAGGAATTAAGCCTTTTTACTGTAGATTTATTCCTTTTTTTACGCTATAGTTATTCAAGTAAAGATCACGGAT
TTAGAAAGGTGAAAAAGCTA

Downstream 100 bases:

>100_bases
ACTATTATTATAGAAGAAAGTGAGCAGAATTCGCTCTGGATTCTGCTCACAATAAACTAGAAAAGGGGCTAAAAAGGGGA
TATGTCAATACCATCGTTTC

Product: lipoprotein ApbE involved in thiamine biosynthesis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKKWKIIISVIILALVVSACGNSSKETKSEPSDSKKLMDQPYSKTDFLMGTVVTLKIYDKGKEDVLDKGFDRIKDLAAKI
TTSDSEKTSEVDKINEQAGKKPVKVSKDVYYLIQEGLKYSENSGGSFDITIGPLTSLWHIGFSDARKPSQAEIDAVLPLI
NYKDVKMNDKDQTVYLEKEGMELDLGAIAKGFITDETLKVFKENKVTTSIIDLGGNIYVQGNNPNGNKWNVGIQDPFSPR
GSVIGKLPESNMSIVTSGIYERYLEVDGKTYHHILDPKTGYPFDNDIAGVSIVSKKSIDGDGLSTATFSKGVKGGMDYIE
QFEGVDAIFISKEKKVYETSGLKGQFELTDKDFQMDTLKK

Sequences:

>Translated_360_residues
MKKWKIIISVIILALVVSACGNSSKETKSEPSDSKKLMDQPYSKTDFLMGTVVTLKIYDKGKEDVLDKGFDRIKDLAAKI
TTSDSEKTSEVDKINEQAGKKPVKVSKDVYYLIQEGLKYSENSGGSFDITIGPLTSLWHIGFSDARKPSQAEIDAVLPLI
NYKDVKMNDKDQTVYLEKEGMELDLGAIAKGFITDETLKVFKENKVTTSIIDLGGNIYVQGNNPNGNKWNVGIQDPFSPR
GSVIGKLPESNMSIVTSGIYERYLEVDGKTYHHILDPKTGYPFDNDIAGVSIVSKKSIDGDGLSTATFSKGVKGGMDYIE
QFEGVDAIFISKEKKVYETSGLKGQFELTDKDFQMDTLKK
>Mature_360_residues
MKKWKIIISVIILALVVSACGNSSKETKSEPSDSKKLMDQPYSKTDFLMGTVVTLKIYDKGKEDVLDKGFDRIKDLAAKI
TTSDSEKTSEVDKINEQAGKKPVKVSKDVYYLIQEGLKYSENSGGSFDITIGPLTSLWHIGFSDARKPSQAEIDAVLPLI
NYKDVKMNDKDQTVYLEKEGMELDLGAIAKGFITDETLKVFKENKVTTSIIDLGGNIYVQGNNPNGNKWNVGIQDPFSPR
GSVIGKLPESNMSIVTSGIYERYLEVDGKTYHHILDPKTGYPFDNDIAGVSIVSKKSIDGDGLSTATFSKGVKGGMDYIE
QFEGVDAIFISKEKKVYETSGLKGQFELTDKDFQMDTLKK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=263, Percent_Identity=26.615969581749, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 39788; Mature: 39788

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWKIIISVIILALVVSACGNSSKETKSEPSDSKKLMDQPYSKTDFLMGTVVTLKIYDK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHEEEEEEEEEEECC
GKEDVLDKGFDRIKDLAAKITTSDSEKTSEVDKINEQAGKKPVKVSKDVYYLIQEGLKYS
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
ENSGGSFDITIGPLTSLWHIGFSDARKPSQAEIDAVLPLINYKDVKMNDKDQTVYLEKEG
CCCCCEEEEEECCHHHHHHCCCHHCCCCCCHHHHHHHHCCCCCCCEECCCCCEEEEECCC
MELDLGAIAKGFITDETLKVFKENKVTTSIIDLGGNIYVQGNNPNGNKWNVGIQDPFSPR
CEEEHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCEEEECCCCCCCCC
GSVIGKLPESNMSIVTSGIYERYLEVDGKTYHHILDPKTGYPFDNDIAGVSIVSKKSIDG
CCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCC
DGLSTATFSKGVKGGMDYIEQFEGVDAIFISKEKKVYETSGLKGQFELTDKDFQMDTLKK
CCCCHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCEEEEECCCCCCHHHCCC
>Mature Secondary Structure
MKKWKIIISVIILALVVSACGNSSKETKSEPSDSKKLMDQPYSKTDFLMGTVVTLKIYDK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHEEEEEEEEEEECC
GKEDVLDKGFDRIKDLAAKITTSDSEKTSEVDKINEQAGKKPVKVSKDVYYLIQEGLKYS
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
ENSGGSFDITIGPLTSLWHIGFSDARKPSQAEIDAVLPLINYKDVKMNDKDQTVYLEKEG
CCCCCEEEEEECCHHHHHHCCCHHCCCCCCHHHHHHHHCCCCCCCEECCCCCEEEEECCC
MELDLGAIAKGFITDETLKVFKENKVTTSIIDLGGNIYVQGNNPNGNKWNVGIQDPFSPR
CEEEHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCEEEECCCCCCCCC
GSVIGKLPESNMSIVTSGIYERYLEVDGKTYHHILDPKTGYPFDNDIAGVSIVSKKSIDG
CCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCC
DGLSTATFSKGVKGGMDYIEQFEGVDAIFISKEKKVYETSGLKGQFELTDKDFQMDTLKK
CCCCHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCEEEEECCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]