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The map label for this gene is mnhD1 [H]

Identifier: 226224933

GI number: 226224933

Start: 2430063

End: 2431547

Strand: Direct

Name: mnhD1 [H]

Synonym: Lm4b_02353

Alternate gene names: 226224933

Gene position: 2430063-2431547 (Clockwise)

Preceding gene: 226224932

Following gene: 226224934

Centisome position: 83.43

GC content: 38.86

Gene sequence:

>1485_bases
ATGAATAATATAATTTTAATGCCGATTTTGATACCTTTCCTTGGAGCAATTACGTTAATGTTGCTTCCGAAAAAAGTAAT
CATTCAACGGGTATTCGCCCTTATTTTTAGTGGTATTTTGGTTGTAGCGACACTTGGACTTGTATTTTATATTCGAGAAA
ACGGGATTACAACTGTGAATATTGGTAACTGGGCGGCTCCTTTTGGGATTACGATGGTTGGCGATATGCTTGCTGTCTTG
CTTACTGCAACGACGAGTATTGTTTTATTCTGCGTGATTCTTTATTCTTTTTATACGATTGGGAAACCACGAGAGAAATT
TCTTTATTATCCAGCGATTCTCTTTATGATTGTTGGGGTTAATGGTTCGTTTTTAACTGGCGATATTTTTAATATGTTCG
TATTCTTTGAAGTTATGTTGATGGCGTCTTATGTACTACTTGTCATTGGTGGGACTCAAGTTCAGCTCAAAGCGACGATT
AAATACTTGTTGATTAATGTGGTTGGTTCCGGATTTTTCGTTGTAGCAATTGCGCTACTTTATTCGATGATTGGAACGCT
CAACATGGCAGATATTTCGCAAAAAATTACGGACTTAAATGGTGCAAATACAGGAATGATTAGCGTTGTCGCGGTTCTGT
TCTTGTTTGTATTCGGACTAAAAGCAGGACTATTCCCGCTTTACTTCTGGCTACCAGGATCTTACTTTGCTCCACCAATT
CCGGTGCTTGCTTTGTTCGGTGGACTTCTGACAAAAGTTGGGGTTTATGCAATTATCCGGACATACACACTATTTTTCAG
TTCGTTAACAGATTTTGTTGTTCCGCTACTTGGAATTCTCGCGATTGTGACGATTGTTCTTGGGGTTATTGGGGCAATTA
GTTACTATGATATGAAGACGATTGTGATTTATAATATTATGATTGCGATTGGTGTTATTCTGTTTAGCGTTTCGATTATG
ACACGCGAATCAATGACTGGTGCTGTCTTTTACTTGATTCACGATATGATTATTAAAGCCGCGCTGTTCCTTATTGTCGG
GATTGTGATGGCGATTACTGGTTATTCTAGTGTAAAAAAATTCAGTGGGTTGATGAGCGTGAAGCCTTCGCTCGGTTGGA
TTTTCTTCATTGCTACTCTTGGCCTTGCAGGAATTCCACCTCTTAGCGGCTTCATTGGAAAATTATTGATTGTGGAAGGT
GCTTTCTCTGCCGGTCAAATAGTCGGCGGAATAATCATCTTACTATCCAGCTTATTCGTCCTTATGTCTTTAATCAAAGT
CTTTACAAAAGGCTTCTGGGGCGAGAAAAAAGGCGTATTCAATTTACAAATTCCGTATAAAAAAATGCTTGTGCCAGTAG
TTATTCTACTTGCGATTTCGATTGGTTATGGTGTGTTTAGTAATGCAATTTATCCGTTTATTGAACAGGCGGTTGACCCT
CTTGTTGACCCTTCTGTTTATATCCATGCGGTGATAAAGGAGTGA

Upstream 100 bases:

>100_bases
AGCTTTGGTGTAACTGCCTTTTTCCTTGTTCTTGCTTATAGAGCGTATCAGGAGCTTGATAGCGAGAGTGTATCCAAGAC
GAGAGGACATGAAGCCGATG

Downstream 100 bases:

>100_bases
TAAAAGATGGCTTTTCAACTTATTCTTAATATAATACTTGCTTGTTTGTGGATGTTTCTTGAGTCATCATTTAGTTTTGC
AACATTTATCATTGGCTTTA

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: Mnh complex subunit D1 [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNNIILMPILIPFLGAITLMLLPKKVIIQRVFALIFSGILVVATLGLVFYIRENGITTVNIGNWAAPFGITMVGDMLAVL
LTATTSIVLFCVILYSFYTIGKPREKFLYYPAILFMIVGVNGSFLTGDIFNMFVFFEVMLMASYVLLVIGGTQVQLKATI
KYLLINVVGSGFFVVAIALLYSMIGTLNMADISQKITDLNGANTGMISVVAVLFLFVFGLKAGLFPLYFWLPGSYFAPPI
PVLALFGGLLTKVGVYAIIRTYTLFFSSLTDFVVPLLGILAIVTIVLGVIGAISYYDMKTIVIYNIMIAIGVILFSVSIM
TRESMTGAVFYLIHDMIIKAALFLIVGIVMAITGYSSVKKFSGLMSVKPSLGWIFFIATLGLAGIPPLSGFIGKLLIVEG
AFSAGQIVGGIIILLSSLFVLMSLIKVFTKGFWGEKKGVFNLQIPYKKMLVPVVILLAISIGYGVFSNAIYPFIEQAVDP
LVDPSVYIHAVIKE

Sequences:

>Translated_494_residues
MNNIILMPILIPFLGAITLMLLPKKVIIQRVFALIFSGILVVATLGLVFYIRENGITTVNIGNWAAPFGITMVGDMLAVL
LTATTSIVLFCVILYSFYTIGKPREKFLYYPAILFMIVGVNGSFLTGDIFNMFVFFEVMLMASYVLLVIGGTQVQLKATI
KYLLINVVGSGFFVVAIALLYSMIGTLNMADISQKITDLNGANTGMISVVAVLFLFVFGLKAGLFPLYFWLPGSYFAPPI
PVLALFGGLLTKVGVYAIIRTYTLFFSSLTDFVVPLLGILAIVTIVLGVIGAISYYDMKTIVIYNIMIAIGVILFSVSIM
TRESMTGAVFYLIHDMIIKAALFLIVGIVMAITGYSSVKKFSGLMSVKPSLGWIFFIATLGLAGIPPLSGFIGKLLIVEG
AFSAGQIVGGIIILLSSLFVLMSLIKVFTKGFWGEKKGVFNLQIPYKKMLVPVVILLAISIGYGVFSNAIYPFIEQAVDP
LVDPSVYIHAVIKE
>Mature_494_residues
MNNIILMPILIPFLGAITLMLLPKKVIIQRVFALIFSGILVVATLGLVFYIRENGITTVNIGNWAAPFGITMVGDMLAVL
LTATTSIVLFCVILYSFYTIGKPREKFLYYPAILFMIVGVNGSFLTGDIFNMFVFFEVMLMASYVLLVIGGTQVQLKATI
KYLLINVVGSGFFVVAIALLYSMIGTLNMADISQKITDLNGANTGMISVVAVLFLFVFGLKAGLFPLYFWLPGSYFAPPI
PVLALFGGLLTKVGVYAIIRTYTLFFSSLTDFVVPLLGILAIVTIVLGVIGAISYYDMKTIVIYNIMIAIGVILFSVSIM
TRESMTGAVFYLIHDMIIKAALFLIVGIVMAITGYSSVKKFSGLMSVKPSLGWIFFIATLGLAGIPPLSGFIGKLLIVEG
AFSAGQIVGGIIILLSSLFVLMSLIKVFTKGFWGEKKGVFNLQIPYKKMLVPVVILLAISIGYGVFSNAIYPFIEQAVDP
LVDPSVYIHAVIKE

Specific function: Mnh complex is a Na(+)/H(+) antiporter involved in Na(+) excretion [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=345, Percent_Identity=22.3188405797101, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788829, Length=422, Percent_Identity=25.8293838862559, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788827, Length=370, Percent_Identity=26.4864864864865, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788613, Length=430, Percent_Identity=24.8837209302326, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788614, Length=479, Percent_Identity=25.2609603340292, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788831, Length=287, Percent_Identity=23.6933797909408, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI2367154, Length=422, Percent_Identity=22.7488151658768, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI145693160, Length=254, Percent_Identity=25.9842519685039, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53688; Mature: 53688

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIILMPILIPFLGAITLMLLPKKVIIQRVFALIFSGILVVATLGLVFYIRENGITTVN
CCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEE
IGNWAAPFGITMVGDMLAVLLTATTSIVLFCVILYSFYTIGKPREKFLYYPAILFMIVGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
NGSFLTGDIFNMFVFFEVMLMASYVLLVIGGTQVQLKATIKYLLINVVGSGFFVVAIALL
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHH
YSMIGTLNMADISQKITDLNGANTGMISVVAVLFLFVFGLKAGLFPLYFWLPGSYFAPPI
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
PVLALFGGLLTKVGVYAIIRTYTLFFSSLTDFVVPLLGILAIVTIVLGVIGAISYYDMKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVIYNIMIAIGVILFSVSIMTRESMTGAVFYLIHDMIIKAALFLIVGIVMAITGYSSVKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
FSGLMSVKPSLGWIFFIATLGLAGIPPLSGFIGKLLIVEGAFSAGQIVGGIIILLSSLFV
HHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LMSLIKVFTKGFWGEKKGVFNLQIPYKKMLVPVVILLAISIGYGVFSNAIYPFIEQAVDP
HHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDPSVYIHAVIKE
CCCCCHHHEEEECC
>Mature Secondary Structure
MNNIILMPILIPFLGAITLMLLPKKVIIQRVFALIFSGILVVATLGLVFYIRENGITTVN
CCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEE
IGNWAAPFGITMVGDMLAVLLTATTSIVLFCVILYSFYTIGKPREKFLYYPAILFMIVGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
NGSFLTGDIFNMFVFFEVMLMASYVLLVIGGTQVQLKATIKYLLINVVGSGFFVVAIALL
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHH
YSMIGTLNMADISQKITDLNGANTGMISVVAVLFLFVFGLKAGLFPLYFWLPGSYFAPPI
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
PVLALFGGLLTKVGVYAIIRTYTLFFSSLTDFVVPLLGILAIVTIVLGVIGAISYYDMKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVIYNIMIAIGVILFSVSIMTRESMTGAVFYLIHDMIIKAALFLIVGIVMAITGYSSVKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
FSGLMSVKPSLGWIFFIATLGLAGIPPLSGFIGKLLIVEGAFSAGQIVGGIIILLSSLFV
HHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LMSLIKVFTKGFWGEKKGVFNLQIPYKKMLVPVVILLAISIGYGVFSNAIYPFIEQAVDP
HHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDPSVYIHAVIKE
CCCCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA