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The map label for this gene is 226224777

Identifier: 226224777

GI number: 226224777

Start: 2252105

End: 2253034

Strand: Reverse

Name: 226224777

Synonym: Lm4b_02195

Alternate gene names: NA

Gene position: 2253034-2252105 (Counterclockwise)

Preceding gene: 226224778

Following gene: 226224774

Centisome position: 77.35

GC content: 41.29

Gene sequence:

>930_bases
ATGAGTTTAACTGAAATGCTACAAATCAAATATCCAATTTTACAAGGGGCAATGGCACAAATTGCCACATATGAATTAGC
TTCCGCGGTATCAAATGTAGGTGGGCTAGGAATTATCGCATCAGGTGGAATGTCAGCAGACACATTACGAGAACAAATTC
GCCTTTGTAAAGAAAAGACAACAAAACCGTTCGCTGTAAATATCATGCTGATGATGCCAAATTGTCCAGAACTTGTCGAT
GTTATTATTGAAGAAGATGTGCGAGTTGTTACAACTGGGGCAGGCACGCCTAAACCATTTATGGAAAAATTTAAAGCAGC
TGGAATTAAAGTTATCGCCGTTATCCCGTCTGTGAAAATTGCTCAAAAAATGGAAGAAATCGGTGTGGACGCTGTTGTTG
CAGAAGGGACAGAAGCAGGTGGACATGTCGGAGAAACAACAACGATGGCACTCGTTCGTCAAGTTGTTTCAGCAGTAAAT
ATTCCTGTCATTGCAGCAGGCGGTATTGCAGATGGTCACGGAATGGCAGCTGTTTATGCGTTAGGCGCAAGCGGTGTCCA
AATCGGCACACTTTTCCTTGTTGCTGAGGAATGTCCGGTTCCAGCTAGCTTTAAACAAGCGGTACTTGACGCAACGGATA
CAAGTACGACTGTAACAGGTCGTCGCAATGGTGCGCCAGTTAGAAGTATTAAAAACCCAATGATTCAAAAATATGTTGAA
CTAGAAAACGAAAATGCCTCCAGAGACAAATTAGAGGAATTAACACTTGGCTCTCTTAGAAAAGCAGTACATGAAGGTGA
CGTTGAGAATGGTTCAGTTATGGCTGGACAAATTTGTGGTATGTTAAAAGAAATTCGTTCCACTAGTGATATTATCGAAA
GCCTCATGAAAGAATCTAAACAAGTTGCAAGCAATTTAGTGATTCAATAA

Upstream 100 bases:

>100_bases
ACTTGCTGCTGTAGGACTTTGCATTAATATTGTTTATAAATTACGTGAGAAAAAACAACCAACTAAAGAATTAGCGTAAA
AATAGAGAGGAGAAAAGAAA

Downstream 100 bases:

>100_bases
TAAAAAGAGTCAGTAATATCCTGGTGGATTTTGCTGACTCTTTTTTTATTTACGTCGATACATTTTTGTTAAGTCTACCA
TCAAATAAATAAGTAGAACA

Product: hypothetical protein

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSLTEMLQIKYPILQGAMAQIATYELASAVSNVGGLGIIASGGMSADTLREQIRLCKEKTTKPFAVNIMLMMPNCPELVD
VIIEEDVRVVTTGAGTPKPFMEKFKAAGIKVIAVIPSVKIAQKMEEIGVDAVVAEGTEAGGHVGETTTMALVRQVVSAVN
IPVIAAGGIADGHGMAAVYALGASGVQIGTLFLVAEECPVPASFKQAVLDATDTSTTVTGRRNGAPVRSIKNPMIQKYVE
LENENASRDKLEELTLGSLRKAVHEGDVENGSVMAGQICGMLKEIRSTSDIIESLMKESKQVASNLVIQ

Sequences:

>Translated_309_residues
MSLTEMLQIKYPILQGAMAQIATYELASAVSNVGGLGIIASGGMSADTLREQIRLCKEKTTKPFAVNIMLMMPNCPELVD
VIIEEDVRVVTTGAGTPKPFMEKFKAAGIKVIAVIPSVKIAQKMEEIGVDAVVAEGTEAGGHVGETTTMALVRQVVSAVN
IPVIAAGGIADGHGMAAVYALGASGVQIGTLFLVAEECPVPASFKQAVLDATDTSTTVTGRRNGAPVRSIKNPMIQKYVE
LENENASRDKLEELTLGSLRKAVHEGDVENGSVMAGQICGMLKEIRSTSDIIESLMKESKQVASNLVIQ
>Mature_308_residues
SLTEMLQIKYPILQGAMAQIATYELASAVSNVGGLGIIASGGMSADTLREQIRLCKEKTTKPFAVNIMLMMPNCPELVDV
IIEEDVRVVTTGAGTPKPFMEKFKAAGIKVIAVIPSVKIAQKMEEIGVDAVVAEGTEAGGHVGETTTMALVRQVVSAVNI
PVIAAGGIADGHGMAAVYALGASGVQIGTLFLVAEECPVPASFKQAVLDATDTSTTVTGRRNGAPVRSIKNPMIQKYVEL
ENENASRDKLEELTLGSLRKAVHEGDVENGSVMAGQICGMLKEIRSTSDIIESLMKESKQVASNLVIQ

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322609, Length=175, Percent_Identity=27.4285714285714, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 32571; Mature: 32440

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTEMLQIKYPILQGAMAQIATYELASAVSNVGGLGIIASGGMSADTLREQIRLCKEKT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCC
TKPFAVNIMLMMPNCPELVDVIIEEDVRVVTTGAGTPKPFMEKFKAAGIKVIAVIPSVKI
CCCEEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCEEEEEECCCHHH
AQKMEEIGVDAVVAEGTEAGGHVGETTTMALVRQVVSAVNIPVIAAGGIADGHGMAAVYA
HHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHEE
LGASGVQIGTLFLVAEECPVPASFKQAVLDATDTSTTVTGRRNGAPVRSIKNPMIQKYVE
ECCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHH
LENENASRDKLEELTLGSLRKAVHEGDVENGSVMAGQICGMLKEIRSTSDIIESLMKESK
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVASNLVIQ
HHHHHCCCC
>Mature Secondary Structure 
SLTEMLQIKYPILQGAMAQIATYELASAVSNVGGLGIIASGGMSADTLREQIRLCKEKT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCC
TKPFAVNIMLMMPNCPELVDVIIEEDVRVVTTGAGTPKPFMEKFKAAGIKVIAVIPSVKI
CCCEEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCEEEEEECCCHHH
AQKMEEIGVDAVVAEGTEAGGHVGETTTMALVRQVVSAVNIPVIAAGGIADGHGMAAVYA
HHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHEE
LGASGVQIGTLFLVAEECPVPASFKQAVLDATDTSTTVTGRRNGAPVRSIKNPMIQKYVE
ECCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHH
LENENASRDKLEELTLGSLRKAVHEGDVENGSVMAGQICGMLKEIRSTSDIIESLMKESK
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVASNLVIQ
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA