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The map label for this gene is cysL [H]

Identifier: 226224753

GI number: 226224753

Start: 2232186

End: 2233040

Strand: Reverse

Name: cysL [H]

Synonym: Lm4b_02171

Alternate gene names: 226224753

Gene position: 2233040-2232186 (Counterclockwise)

Preceding gene: 226224756

Following gene: 226224752

Centisome position: 76.67

GC content: 37.31

Gene sequence:

>855_bases
ATGGATGAGGCATTAAGAACATATATCCGAGTAGTAGAATTGCAAAGTTTTACAAAAGCAGCTGAAGAATTACACATTTC
GCAACCCGCTGTATCGTTGCAATTAAAGAAATTAGAAGAGATTTATGAAACAGAGCTTATTTACAGGCAGGCTAAAAAGT
TTATTTTAACAGCAACAGGAGAAATTTTATATCACCGTGCCAAGCAATTAGAAGGTCTATATAAACAAGTGGAAGATGAA
ATAAGTTTATATCACCATCATTTAAAAGGTCGACTTCGAATTGGTGCTAGTTTTACGATAGGAGAGTATTTTTTGCCAGA
AGTGATTGCGAAATTTCATGCCCTTTATCCGGATATTACAATAGAACTAATCATTGAAAATACCGCGAAAATTGCTGACA
AAGTGGAACTTCTTCAAGTGGACACAGGGCTTATTGAAGGCCAGGTAAGTAAGAAAGATTTGGAGATAAGTGCTTTTTCG
GATGATGAGATGTGTATCGTTGGTCGAGCAGACGGTTCACTTACCGAAATCGAACAAGGAGCAACTTGGATTGCGCGAGA
AGAAGGCTCAGGAACGCGTGAATATTTGGATCATGTATTAAGTACCAATGGCTGGAATGTTGCTGAAAAAGTTGTTGCTT
GGAGTAATATGGCGGTCAAACAAATGGTTCTTGAAGGAATGGGCTACACCGTTATTTCTAAGTGTGTTGTTCAAACAGAA
ATTACATCAGGAAAACTGCAGACGTTTAATGAGGCTGGTAGCTTAATGCGGAAGTTTTCGGTACTAAAAAACAGACAAAG
ACTTGAAAATCGAATTGCTGAAACCTTTTTAACATTTTTAAAGGAAAACCAGTAA

Upstream 100 bases:

>100_bases
TCCCCCTTTCTTTTATACTTTTACTTTACCATTCTTGATTTTATTAGTAAAATTTATATTAATTATATTATTGATAAGTT
ATTTTTATGGAGGTGTTACT

Downstream 100 bases:

>100_bases
ATAAAACTAGCTTTTTAATTAACTAGTGATTACAATGAGAAAGAATACATTTTAGAAAGGGAGAAGCAATGAAAGAATAT
TTTATAGATCGTTCCTATAA

Product: transcription regulator LysR family

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MDEALRTYIRVVELQSFTKAAEELHISQPAVSLQLKKLEEIYETELIYRQAKKFILTATGEILYHRAKQLEGLYKQVEDE
ISLYHHHLKGRLRIGASFTIGEYFLPEVIAKFHALYPDITIELIIENTAKIADKVELLQVDTGLIEGQVSKKDLEISAFS
DDEMCIVGRADGSLTEIEQGATWIAREEGSGTREYLDHVLSTNGWNVAEKVVAWSNMAVKQMVLEGMGYTVISKCVVQTE
ITSGKLQTFNEAGSLMRKFSVLKNRQRLENRIAETFLTFLKENQ

Sequences:

>Translated_284_residues
MDEALRTYIRVVELQSFTKAAEELHISQPAVSLQLKKLEEIYETELIYRQAKKFILTATGEILYHRAKQLEGLYKQVEDE
ISLYHHHLKGRLRIGASFTIGEYFLPEVIAKFHALYPDITIELIIENTAKIADKVELLQVDTGLIEGQVSKKDLEISAFS
DDEMCIVGRADGSLTEIEQGATWIAREEGSGTREYLDHVLSTNGWNVAEKVVAWSNMAVKQMVLEGMGYTVISKCVVQTE
ITSGKLQTFNEAGSLMRKFSVLKNRQRLENRIAETFLTFLKENQ
>Mature_284_residues
MDEALRTYIRVVELQSFTKAAEELHISQPAVSLQLKKLEEIYETELIYRQAKKFILTATGEILYHRAKQLEGLYKQVEDE
ISLYHHHLKGRLRIGASFTIGEYFLPEVIAKFHALYPDITIELIIENTAKIADKVELLQVDTGLIEGQVSKKDLEISAFS
DDEMCIVGRADGSLTEIEQGATWIAREEGSGTREYLDHVLSTNGWNVAEKVVAWSNMAVKQMVLEGMGYTVISKCVVQTE
ITSGKLQTFNEAGSLMRKFSVLKNRQRLENRIAETFLTFLKENQ

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=282, Percent_Identity=31.5602836879433, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI145693105, Length=212, Percent_Identity=25, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=270, Percent_Identity=28.5185185185185, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788748, Length=238, Percent_Identity=25.6302521008403, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=25.6578947368421, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788887, Length=178, Percent_Identity=29.2134831460674, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790399, Length=278, Percent_Identity=24.1007194244604, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=120, Percent_Identity=28.3333333333333, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789639, Length=136, Percent_Identity=28.6764705882353, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789204, Length=254, Percent_Identity=24.8031496062992, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI145693193, Length=132, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788297, Length=168, Percent_Identity=27.3809523809524, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789506, Length=251, Percent_Identity=22.7091633466135, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789173, Length=258, Percent_Identity=24.8062015503876, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1788626, Length=139, Percent_Identity=30.9352517985612, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32279; Mature: 32279

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEALRTYIRVVELQSFTKAAEELHISQPAVSLQLKKLEEIYETELIYRQAKKFILTATG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EILYHRAKQLEGLYKQVEDEISLYHHHLKGRLRIGASFTIGEYFLPEVIAKFHALYPDIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEHHHHHHHHHHHHHHHHCCCCE
IELIIENTAKIADKVELLQVDTGLIEGQVSKKDLEISAFSDDEMCIVGRADGSLTEIEQG
EEEEECCHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHCC
ATWIAREEGSGTREYLDHVLSTNGWNVAEKVVAWSNMAVKQMVLEGMGYTVISKCVVQTE
CEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ITSGKLQTFNEAGSLMRKFSVLKNRQRLENRIAETFLTFLKENQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDEALRTYIRVVELQSFTKAAEELHISQPAVSLQLKKLEEIYETELIYRQAKKFILTATG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EILYHRAKQLEGLYKQVEDEISLYHHHLKGRLRIGASFTIGEYFLPEVIAKFHALYPDIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEHHHHHHHHHHHHHHHHCCCCE
IELIIENTAKIADKVELLQVDTGLIEGQVSKKDLEISAFSDDEMCIVGRADGSLTEIEQG
EEEEECCHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHCC
ATWIAREEGSGTREYLDHVLSTNGWNVAEKVVAWSNMAVKQMVLEGMGYTVISKCVVQTE
CEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ITSGKLQTFNEAGSLMRKFSVLKNRQRLENRIAETFLTFLKENQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]