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The map label for this gene is mraW

Identifier: 226224647

GI number: 226224647

Start: 2123748

End: 2124686

Strand: Reverse

Name: mraW

Synonym: Lm4b_02062

Alternate gene names: 226224647

Gene position: 2124686-2123748 (Counterclockwise)

Preceding gene: 226224648

Following gene: 226224646

Centisome position: 72.95

GC content: 38.66

Gene sequence:

>939_bases
ATGTTTAAACACGAAACCGTATTACTACATGAAACAGTCGATATGCTTGAAGTAAAACCAGACGGAATCTATGTCGATGC
AACACTTGGCGGCGCAGGTCACTCCGAGTATTTACTAAATAAGCTTAATGAAAAAGGGCATTTATTTGCATTTGACCAAG
ATCAAACAGCAATTGATAATGCAAAAATTAAACTAGCAGATTATAGCGATAAAGTGACTTTTATTAAAGCCAATTTTCGC
GATATGAAAGAAGCATTAAATGAGCGTGGTATCGAAGCGGTTGATGGGATTTTGTATGATTTAGGCGTTTCATCCCCACA
ACTAGACGAGAGAGAACGTGGTTTTAGTTATCACCAAGACGCTGCACTGGATATGCGAATGGATCAAGAACAAGAATTGA
CAGCAAAAATAGTTGTTAACGAATGGTCTTATCAAGATTTAATCCGTATCTTTTTCCAATATGGCGAAGAAAAATTTTCG
AAACAAATTGCTCGTGAAATCGAACGTCGTCGTGAAGTAAAGCCAATTGAAACAACAGGCGAACTAGTAGATATTATCAA
AACAGCTATCCCGGCTCCAGCAAGAAGAAAAGGCGGACATCCAGGGAAACGCACTTTTCAAGCCATCCGAATTGCCGTTA
ACGACGAACTCGGTGCAGTAGAAGATTCGCTTGAAAAAGCCTTAACTTTATTAAAACCAGGCGGTAGAATTAGCGTAATT
ACGTTTCATTCTTTAGAAGACCGCATTACAAAACACTTATTCCAAGAAGCGACAAAAGGACCAGATTTACCACCAGGTCT
TCCTGTCATTCCAGATGAATATAAACCAGATTTCAAACTTGCGACAAGAAAACCAATCGTACCAAGTGAAGAAGAACTGG
AACAAAATAACCGAGCGCGTTCAGCAAAATTACGTGTGATCGAAAAAATTATCAAATAG

Upstream 100 bases:

>100_bases
GAGTAAATCAGAGTGGGATGATGTCTTTAACGAGGCAGAAGAATCTTTTGCTGATTTAGCCGAAAATATGATTGGCTTTG
ATATTTAAGGGGGTAAACGC

Downstream 100 bases:

>100_bases
CAGGAGTTGAAAAAATGTGAGCAATGTCGCCTATAAATCGAATCTCGAGCCAAATAGAGTACATAGAGAAGCGGAACAAC
CTAAAAAACAAATCCTAAAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MFKHETVLLHETVDMLEVKPDGIYVDATLGGAGHSEYLLNKLNEKGHLFAFDQDQTAIDNAKIKLADYSDKVTFIKANFR
DMKEALNERGIEAVDGILYDLGVSSPQLDERERGFSYHQDAALDMRMDQEQELTAKIVVNEWSYQDLIRIFFQYGEEKFS
KQIAREIERRREVKPIETTGELVDIIKTAIPAPARRKGGHPGKRTFQAIRIAVNDELGAVEDSLEKALTLLKPGGRISVI
TFHSLEDRITKHLFQEATKGPDLPPGLPVIPDEYKPDFKLATRKPIVPSEEELEQNNRARSAKLRVIEKIIK

Sequences:

>Translated_312_residues
MFKHETVLLHETVDMLEVKPDGIYVDATLGGAGHSEYLLNKLNEKGHLFAFDQDQTAIDNAKIKLADYSDKVTFIKANFR
DMKEALNERGIEAVDGILYDLGVSSPQLDERERGFSYHQDAALDMRMDQEQELTAKIVVNEWSYQDLIRIFFQYGEEKFS
KQIAREIERRREVKPIETTGELVDIIKTAIPAPARRKGGHPGKRTFQAIRIAVNDELGAVEDSLEKALTLLKPGGRISVI
TFHSLEDRITKHLFQEATKGPDLPPGLPVIPDEYKPDFKLATRKPIVPSEEELEQNNRARSAKLRVIEKIIK
>Mature_312_residues
MFKHETVLLHETVDMLEVKPDGIYVDATLGGAGHSEYLLNKLNEKGHLFAFDQDQTAIDNAKIKLADYSDKVTFIKANFR
DMKEALNERGIEAVDGILYDLGVSSPQLDERERGFSYHQDAALDMRMDQEQELTAKIVVNEWSYQDLIRIFFQYGEEKFS
KQIAREIERRREVKPIETTGELVDIIKTAIPAPARRKGGHPGKRTFQAIRIAVNDELGAVEDSLEKALTLLKPGGRISVI
TFHSLEDRITKHLFQEATKGPDLPPGLPVIPDEYKPDFKLATRKPIVPSEEELEQNNRARSAKLRVIEKIIK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=34.7181008902077, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=37.2340425531915, Blast_Score=104, Evalue=9e-23,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=44.5859872611465, Blast_Score=254, Evalue=4e-69,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=35.7357357357357, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=35.7357357357357, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35451; Mature: 35451

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKHETVLLHETVDMLEVKPDGIYVDATLGGAGHSEYLLNKLNEKGHLFAFDQDQTAIDN
CCCCCEEEHHHHHHHHEECCCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCCCCCC
AKIKLADYSDKVTFIKANFRDMKEALNERGIEAVDGILYDLGVSSPQLDERERGFSYHQD
CEEEEECCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCC
AALDMRMDQEQELTAKIVVNEWSYQDLIRIFFQYGEEKFSKQIAREIERRREVKPIETTG
CHHHCCCCCCHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ELVDIIKTAIPAPARRKGGHPGKRTFQAIRIAVNDELGAVEDSLEKALTLLKPGGRISVI
HHHHHHHHHCCCCHHHCCCCCCHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRITKHLFQEATKGPDLPPGLPVIPDEYKPDFKLATRKPIVPSEEELEQNNRAR
EECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHH
SAKLRVIEKIIK
HHHHHHHHHHHC
>Mature Secondary Structure
MFKHETVLLHETVDMLEVKPDGIYVDATLGGAGHSEYLLNKLNEKGHLFAFDQDQTAIDN
CCCCCEEEHHHHHHHHEECCCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCCCCCC
AKIKLADYSDKVTFIKANFRDMKEALNERGIEAVDGILYDLGVSSPQLDERERGFSYHQD
CEEEEECCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCC
AALDMRMDQEQELTAKIVVNEWSYQDLIRIFFQYGEEKFSKQIAREIERRREVKPIETTG
CHHHCCCCCCHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ELVDIIKTAIPAPARRKGGHPGKRTFQAIRIAVNDELGAVEDSLEKALTLLKPGGRISVI
HHHHHHHHHCCCCHHHCCCCCCHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRITKHLFQEATKGPDLPPGLPVIPDEYKPDFKLATRKPIVPSEEELEQNNRAR
EECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHH
SAKLRVIEKIIK
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]