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The map label for this gene is nifS [H]

Identifier: 226224619

GI number: 226224619

Start: 2090109

End: 2091215

Strand: Reverse

Name: nifS [H]

Synonym: Lm4b_02034

Alternate gene names: 226224619

Gene position: 2091215-2090109 (Counterclockwise)

Preceding gene: 226224623

Following gene: 226224618

Centisome position: 71.8

GC content: 38.84

Gene sequence:

>1107_bases
ATGATTTACTTTGACCATGCGGCTACTACGAAAATGAGTGAAGCTTCTTTACAAGTTTTTATGGATGCATCGCGAGAATT
CTTTGCTAATTCGGAGAGTTTACATGATGCTGGGACGAAATCGGCGGCCCTTTTAGAAAAATGCCGGGAAAGTTTTTCTG
AGTTATTACAAGTTCCAAACCGCGGGATTTTATTTACGAGTGGTGGAACAGAGAGTAATCAAATTGCCATCCAAACGCTA
ATCCATACAACTGATAAGAAAGAAGTGCTCGTAAGTCCGCTAGAACACGCTTCAGTTTGGCAGCAACTAATGGCACTCGA
ACAAGCGGGGAAGTGTCGGATTAAAGTATTACCAGTTGATTCGTTTGGGCAAGTAAATCCAGCTACTTTAGAAAAAATGA
TTTCTGCTGAGACGGGGCTTATTATTATTCAACATGTTAATTCGGAAATCGGAACCATCCAACCAATCGCGGAACTAGCT
CATATTGCTAAAAAAGCAGGTGTCTTTTTTCATACCGATATTGTTCAATCCTTTGGAAAAATTGATTTAGATTTAAGTGA
TGCCACTAGTTTTAGTATTTCATCGCATAAAATTTATGGACCAAAAGGGGCGGGAATACTTTTTATGAAACCAGATTCTC
CTTTGCAGGCAGTTTTGCCAGATGTACATCATGAATTTGGCTTTAGACCGGGGACAGTAAATGTTCCAGCTATTGCTGCC
TTTACAACTGCCGCCTATGATATAATAGAAAATAGAGAACAAGAAGCGATTAGAGCTATGAAACTAAAAGCTGCGATTTG
CGACACATTAGAAGCGCGAGTAGAAGTGGAAGGTGGACCCAATACATCGCCGTATATTTTAGGACTTACTTTGCCAAATA
TGCAAGGTCAGGAAGCGTTACTTGCTTTAAACGAAGCAGATATTCAAATCTCGACGACAAGTGCATGTAGTTTACGTGAT
TTAGCTCCGTCCAGAACATTAATCGCTACTGGAAAAACAACAGAAGAAGCAAACCGTTTTATCCGCTTATCTTTTGGAAG
AGAAAATGAATTAAACGATAGTATTATTTTTAAAGAAGAAATCGATAAATTATTACGAAAAAGGTGA

Upstream 100 bases:

>100_bases
AATTGTATACTTCTTAATGAATATACAGATGTCTTGTCATCTATAATCACATAAAATCAAACTAAATTCAAGTATAAAAT
TTTAAGGGAGAGATAAAGAG

Downstream 100 bases:

>100_bases
TAAGATGAAAAAAATATTAGGAGATACGCGTAGGCAAATGATTTTAAAATGGCTAAAAGAAGCCGAGGCGCCAATTTCTG
GAAATCAATTAGCACAGAAA

Product: NifS-like protein required for NAD biosynthesis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MIYFDHAATTKMSEASLQVFMDASREFFANSESLHDAGTKSAALLEKCRESFSELLQVPNRGILFTSGGTESNQIAIQTL
IHTTDKKEVLVSPLEHASVWQQLMALEQAGKCRIKVLPVDSFGQVNPATLEKMISAETGLIIIQHVNSEIGTIQPIAELA
HIAKKAGVFFHTDIVQSFGKIDLDLSDATSFSISSHKIYGPKGAGILFMKPDSPLQAVLPDVHHEFGFRPGTVNVPAIAA
FTTAAYDIIENREQEAIRAMKLKAAICDTLEARVEVEGGPNTSPYILGLTLPNMQGQEALLALNEADIQISTTSACSLRD
LAPSRTLIATGKTTEEANRFIRLSFGRENELNDSIIFKEEIDKLLRKR

Sequences:

>Translated_368_residues
MIYFDHAATTKMSEASLQVFMDASREFFANSESLHDAGTKSAALLEKCRESFSELLQVPNRGILFTSGGTESNQIAIQTL
IHTTDKKEVLVSPLEHASVWQQLMALEQAGKCRIKVLPVDSFGQVNPATLEKMISAETGLIIIQHVNSEIGTIQPIAELA
HIAKKAGVFFHTDIVQSFGKIDLDLSDATSFSISSHKIYGPKGAGILFMKPDSPLQAVLPDVHHEFGFRPGTVNVPAIAA
FTTAAYDIIENREQEAIRAMKLKAAICDTLEARVEVEGGPNTSPYILGLTLPNMQGQEALLALNEADIQISTTSACSLRD
LAPSRTLIATGKTTEEANRFIRLSFGRENELNDSIIFKEEIDKLLRKR
>Mature_368_residues
MIYFDHAATTKMSEASLQVFMDASREFFANSESLHDAGTKSAALLEKCRESFSELLQVPNRGILFTSGGTESNQIAIQTL
IHTTDKKEVLVSPLEHASVWQQLMALEQAGKCRIKVLPVDSFGQVNPATLEKMISAETGLIIIQHVNSEIGTIQPIAELA
HIAKKAGVFFHTDIVQSFGKIDLDLSDATSFSISSHKIYGPKGAGILFMKPDSPLQAVLPDVHHEFGFRPGTVNVPAIAA
FTTAAYDIIENREQEAIRAMKLKAAICDTLEARVEVEGGPNTSPYILGLTLPNMQGQEALLALNEADIQISTTSACSLRD
LAPSRTLIATGKTTEEANRFIRLSFGRENELNDSIIFKEEIDKLLRKR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Seems to be required for NAD biosynthesis [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=377, Percent_Identity=32.6259946949602, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI156713448, Length=399, Percent_Identity=27.5689223057644, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI48994898, Length=386, Percent_Identity=32.9015544041451, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787970, Length=233, Percent_Identity=26.6094420600858, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789175, Length=220, Percent_Identity=26.3636363636364, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25143064, Length=360, Percent_Identity=33.3333333333333, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17533177, Length=317, Percent_Identity=25.5520504731861, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319831, Length=375, Percent_Identity=30.6666666666667, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI20129463, Length=365, Percent_Identity=33.1506849315069, Blast_Score=175, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40269; Mature: 40269

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDHAATTKMSEASLQVFMDASREFFANSESLHDAGTKSAALLEKCRESFSELLQVPN
CEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCC
RGILFTSGGTESNQIAIQTLIHTTDKKEVLVSPLEHASVWQQLMALEQAGKCRIKVLPVD
CCEEEECCCCCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECC
SFGQVNPATLEKMISAETGLIIIQHVNSEIGTIQPIAELAHIAKKAGVFFHTDIVQSFGK
CCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHCCC
IDLDLSDATSFSISSHKIYGPKGAGILFMKPDSPLQAVLPDVHHEFGFRPGTVNVPAIAA
EEEEECCCCEEEECCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
FTTAAYDIIENREQEAIRAMKLKAAICDTLEARVEVEGGPNTSPYILGLTLPNMQGQEAL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCCCCE
LALNEADIQISTTSACSLRDLAPSRTLIATGKTTEEANRFIRLSFGRENELNDSIIFKEE
EEEECCCEEEEECCCCCHHHCCCCCEEEEECCCHHHCCCEEEEECCCCCCCCCCEEEHHH
IDKLLRKR
HHHHHHCC
>Mature Secondary Structure
MIYFDHAATTKMSEASLQVFMDASREFFANSESLHDAGTKSAALLEKCRESFSELLQVPN
CEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCC
RGILFTSGGTESNQIAIQTLIHTTDKKEVLVSPLEHASVWQQLMALEQAGKCRIKVLPVD
CCEEEECCCCCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECC
SFGQVNPATLEKMISAETGLIIIQHVNSEIGTIQPIAELAHIAKKAGVFFHTDIVQSFGK
CCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHCCC
IDLDLSDATSFSISSHKIYGPKGAGILFMKPDSPLQAVLPDVHHEFGFRPGTVNVPAIAA
EEEEECCCCEEEECCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
FTTAAYDIIENREQEAIRAMKLKAAICDTLEARVEVEGGPNTSPYILGLTLPNMQGQEAL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCCCCE
LALNEADIQISTTSACSLRDLAPSRTLIATGKTTEEANRFIRLSFGRENELNDSIIFKEE
EEEECCCEEEEECCCCCHHHCCCCCEEEEECCCHHHCCCEEEEECCCCCCCCCCEEEHHH
IDKLLRKR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]