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The map label for this gene is rluB [H]

Identifier: 226224554

GI number: 226224554

Start: 2018225

End: 2018959

Strand: Reverse

Name: rluB [H]

Synonym: Lm4b_01968

Alternate gene names: 226224554

Gene position: 2018959-2018225 (Counterclockwise)

Preceding gene: 226224555

Following gene: 226224553

Centisome position: 69.32

GC content: 36.46

Gene sequence:

>735_bases
ATGGAACGTTTACAAAAAGTGATTGCAAATGCAGGTATTACTTCAAGAAGAAAAGCAGAAAAGCTTATTCAAGAAGGTAA
AGTAACAGTGAACGGGAAAGTAGTCACAGAATTAGGTGTGAAAGTTAGTGGTACGGAGCGAATTGAAGTTGAGGGAATTC
AACTAACAAAAGAAGAACATCGCTATTTCCTTTTTTATAAGCCAAGAGGAACGGTTTCGGCTGTAACAGATGATAAAGGT
CGTACAACTGTAGCGGATTATTTTGCAGATATTCCTGAAAGATTATACCCAGTAGGGCGACTTGATTATGATACATCTGG
TTTATTATTAATGACCAATGATGGGGATTTCGCTAACTTACTAATGCATCCGAAAAATGAAGTTTCCAAAACATATATCG
CACGAATCAAAGGTATTCCTGAACGTGAAGTGATTCGTCAGTTAGAACGTGGTGTGGTGATTGATGGTCGCAAAACAGCC
CCAGCAAAAGTAAAAGTACGTTCTTCTGATAAAACGAAAGATAAAGCTATTGTAGAAATCACGATTCATGAAGGTAGAAA
TCGTCAAGTCCGTAAAATGTTTGAGGCTGTTGGTTTTGAAGTGCAAAAATTATCAAGGGAAGAATACTCGTTCTTGAATT
TACGCGGTTTAAACGCAGGTGAGCGTAGAGAATTATCACATCATGAAGTAAAACAATTAAAAACAGAAGCAAGATTTGGT
AAAAATAAAAAGTAA

Upstream 100 bases:

>100_bases
CAAAACTTGCTGATCCAGCGACAGATGAACCAGATCAGAATGAAATGGACCTGTTTTTTGACCGGTTTAACCAAAGTAAA
GAACAGGAGGAGGAATAAGA

Downstream 100 bases:

>100_bases
CTTTTCATACAAAATATACTTTCTAATAGCGTGTTTTGTGAAAAACATGTATAATTTAAATCGTAGGAAATTTGGTATAG
GCAATTGTATGGTTTTTTCG

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MERLQKVIANAGITSRRKAEKLIQEGKVTVNGKVVTELGVKVSGTERIEVEGIQLTKEEHRYFLFYKPRGTVSAVTDDKG
RTTVADYFADIPERLYPVGRLDYDTSGLLLMTNDGDFANLLMHPKNEVSKTYIARIKGIPEREVIRQLERGVVIDGRKTA
PAKVKVRSSDKTKDKAIVEITIHEGRNRQVRKMFEAVGFEVQKLSREEYSFLNLRGLNAGERRELSHHEVKQLKTEARFG
KNKK

Sequences:

>Translated_244_residues
MERLQKVIANAGITSRRKAEKLIQEGKVTVNGKVVTELGVKVSGTERIEVEGIQLTKEEHRYFLFYKPRGTVSAVTDDKG
RTTVADYFADIPERLYPVGRLDYDTSGLLLMTNDGDFANLLMHPKNEVSKTYIARIKGIPEREVIRQLERGVVIDGRKTA
PAKVKVRSSDKTKDKAIVEITIHEGRNRQVRKMFEAVGFEVQKLSREEYSFLNLRGLNAGERRELSHHEVKQLKTEARFG
KNKK
>Mature_244_residues
MERLQKVIANAGITSRRKAEKLIQEGKVTVNGKVVTELGVKVSGTERIEVEGIQLTKEEHRYFLFYKPRGTVSAVTDDKG
RTTVADYFADIPERLYPVGRLDYDTSGLLLMTNDGDFANLLMHPKNEVSKTYIARIKGIPEREVIRQLERGVVIDGRKTA
PAKVKVRSSDKTKDKAIVEITIHEGRNRQVRKMFEAVGFEVQKLSREEYSFLNLRGLNAGERRELSHHEVKQLKTEARFG
KNKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=243, Percent_Identity=38.2716049382716, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1790453, Length=235, Percent_Identity=36.1702127659575, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=35.0210970464135, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87081838, Length=183, Percent_Identity=33.879781420765, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27798; Mature: 27798

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKVIANAGITSRRKAEKLIQEGKVTVNGKVVTELGVKVSGTERIEVEGIQLTKEEH
CHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCEEEEEECEEECCCCEEEEEEEEEECCCC
RYFLFYKPRGTVSAVTDDKGRTTVADYFADIPERLYPVGRLDYDTSGLLLMTNDGDFANL
EEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEE
LMHPKNEVSKTYIARIKGIPEREVIRQLERGVVIDGRKTAPAKVKVRSSDKTKDKAIVEI
EECCCHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCEEEEE
TIHEGRNRQVRKMFEAVGFEVQKLSREEYSFLNLRGLNAGERRELSHHEVKQLKTEARFG
EEECCCCHHHHHHHHHHCHHHHHHHHHHHCEEEEECCCCCCHHCCHHHHHHHHHHHHHCC
KNKK
CCCC
>Mature Secondary Structure
MERLQKVIANAGITSRRKAEKLIQEGKVTVNGKVVTELGVKVSGTERIEVEGIQLTKEEH
CHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCEEEEEECEEECCCCEEEEEEEEEECCCC
RYFLFYKPRGTVSAVTDDKGRTTVADYFADIPERLYPVGRLDYDTSGLLLMTNDGDFANL
EEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEE
LMHPKNEVSKTYIARIKGIPEREVIRQLERGVVIDGRKTAPAKVKVRSSDKTKDKAIVEI
EECCCHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCEEEEE
TIHEGRNRQVRKMFEAVGFEVQKLSREEYSFLNLRGLNAGERRELSHHEVKQLKTEARFG
EEECCCCHHHHHHHHHHCHHHHHHHHHHHCEEEEECCCCCCHHCCHHHHHHHHHHHHHCC
KNKK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]