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The map label for this gene is yurR [H]

Identifier: 226224514

GI number: 226224514

Start: 1970496

End: 1971602

Strand: Reverse

Name: yurR [H]

Synonym: Lm4b_01927

Alternate gene names: 226224514

Gene position: 1971602-1970496 (Counterclockwise)

Preceding gene: 226224522

Following gene: 226224512

Centisome position: 67.69

GC content: 43.36

Gene sequence:

>1107_bases
ATGCAAAAAATAGTGATTATCGGCGGGGGAATTGTTGGAGCTAGTGCCGCCTATTTATTGTCAAAAGAAAACGTGCAAGT
AACGCTAATTGATTCAGATGAACCGGGACAAGCAACGCGTGCTGCAGCCGGAATAATTTGCCCGTGGCTTTCTAAAAGGC
GAAACAAATATTGGTATGAGCTTGCAAAAAATAGTGCCGCCTTTTATAAGGAATTGGCGGGAACGCTCGAAGGAGATACT
GGGCGGAATTCGGGCTATAAACAAGTCGGTGTGCTCGCACTTCGAAAGACAGAAGAAAAAGTAACAGAACTGTTTAATCT
TGCGAATGAACGACACCTCGATGCGGAAGTTATGGGTGAAATTGCCAAGTTGTCAGAAGAGGAAACAAAGCAAAAATTTC
CGTTAGTTAAACCGGGATTTGGCTCGGTCTATGTAAGCGGAGCAGCAAGAGTGAACGGTAGTTTATTTTGTGAAAGGCTA
CTTTATGCCGCAAAAGAAAATGGGGTAAAAATCAAATCGGGGCGAGCGCATTTTTCTACTGACGGAAAAGTTCGTGTTGA
CGGGGAAGATGAACATTACGACAAATTAATCATTGCAGCAGGCGCATGGTTAAAAGAATTGCTAGACGAGGCGAGTTTTC
ATACAGAAGTTTTAGCGCAAAAAGGGCAATTGCTGGAACTTGATTTTAGCGAATTTCAAACGGATGAATGGCCAGTAATT
TTACCACCGAGCGCGAAATCAATTGTTCCTTTTGATAATGGGAAAATTATCGTCGGTGCAACGCATGAAAAAGCTGCTGG
CTTCGATACGAAACCAACAGCAGAAGGTAAGGCAGAAATACTAACAGAAGTCAGCCAGTTTATGGAAGGGGATTTAGCGA
GTAAAGTGGCCCATGTGGCGGTCGGAACTAGACCATACACGCCTGATTTTGCGCCACTTATTGGTCAACTTCCTGGATTT
GAGTCAGTCTTTTTAGCGAATGGTCTTGGTGCTTCTGGTTTAACTACCGGGCCTTATGTTGGCAAACTTTTAGCGGATTT
AGCACTTGGAAACACCGTCGATTTGGCTTTAGAAAACTATGAGCCAAGTAAATACATTAGTAGATAG

Upstream 100 bases:

>100_bases
GGATTTTTAGTAGTTTGTAAACTTAACTATTTTCACATATTATTACAAAGAGTTATAAAAAAGTCAACTTGTGTCACAAT
TGATATAGAGGGGTGTAACG

Downstream 100 bases:

>100_bases
GATTCTTGTTTTCATCGAGTGTGAAACCTTCTCCCATCACATCATGAACGACACTGACGGAAACAAAGGCATGTGGGTCA
ATTGCTTGAACAATATTTTT

Product: oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MQKIVIIGGGIVGASAAYLLSKENVQVTLIDSDEPGQATRAAAGIICPWLSKRRNKYWYELAKNSAAFYKELAGTLEGDT
GRNSGYKQVGVLALRKTEEKVTELFNLANERHLDAEVMGEIAKLSEEETKQKFPLVKPGFGSVYVSGAARVNGSLFCERL
LYAAKENGVKIKSGRAHFSTDGKVRVDGEDEHYDKLIIAAGAWLKELLDEASFHTEVLAQKGQLLELDFSEFQTDEWPVI
LPPSAKSIVPFDNGKIIVGATHEKAAGFDTKPTAEGKAEILTEVSQFMEGDLASKVAHVAVGTRPYTPDFAPLIGQLPGF
ESVFLANGLGASGLTTGPYVGKLLADLALGNTVDLALENYEPSKYISR

Sequences:

>Translated_368_residues
MQKIVIIGGGIVGASAAYLLSKENVQVTLIDSDEPGQATRAAAGIICPWLSKRRNKYWYELAKNSAAFYKELAGTLEGDT
GRNSGYKQVGVLALRKTEEKVTELFNLANERHLDAEVMGEIAKLSEEETKQKFPLVKPGFGSVYVSGAARVNGSLFCERL
LYAAKENGVKIKSGRAHFSTDGKVRVDGEDEHYDKLIIAAGAWLKELLDEASFHTEVLAQKGQLLELDFSEFQTDEWPVI
LPPSAKSIVPFDNGKIIVGATHEKAAGFDTKPTAEGKAEILTEVSQFMEGDLASKVAHVAVGTRPYTPDFAPLIGQLPGF
ESVFLANGLGASGLTTGPYVGKLLADLALGNTVDLALENYEPSKYISR
>Mature_368_residues
MQKIVIIGGGIVGASAAYLLSKENVQVTLIDSDEPGQATRAAAGIICPWLSKRRNKYWYELAKNSAAFYKELAGTLEGDT
GRNSGYKQVGVLALRKTEEKVTELFNLANERHLDAEVMGEIAKLSEEETKQKFPLVKPGFGSVYVSGAARVNGSLFCERL
LYAAKENGVKIKSGRAHFSTDGKVRVDGEDEHYDKLIIAAGAWLKELLDEASFHTEVLAQKGQLLELDFSEFQTDEWPVI
LPPSAKSIVPFDNGKIIVGATHEKAAGFDTKPTAEGKAEILTEVSQFMEGDLASKVAHVAVGTRPYTPDFAPLIGQLPGF
ESVFLANGLGASGLTTGPYVGKLLADLALGNTVDLALENYEPSKYISR

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 39772; Mature: 39772

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIVIIGGGIVGASAAYLLSKENVQVTLIDSDEPGQATRAAAGIICPWLSKRRNKYWYE
CCEEEEEECCHHHHHHEEEEECCCEEEEEEECCCCCHHHHHHHHHCCHHHHHHCCHHHHH
LAKNSAAFYKELAGTLEGDTGRNSGYKQVGVLALRKTEEKVTELFNLANERHLDAEVMGE
HHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHHCCCCCCHHHHHH
IAKLSEEETKQKFPLVKPGFGSVYVSGAARVNGSLFCERLLYAAKENGVKIKSGRAHFST
HHHHHHHHHHHHCCCCCCCCCCEEEECCHHCCHHHHHHHHHHHHHCCCEEEECCCEEECC
DGKVRVDGEDEHYDKLIIAAGAWLKELLDEASFHTEVLAQKGQLLELDFSEFQTDEWPVI
CCEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCEE
LPPSAKSIVPFDNGKIIVGATHEKAAGFDTKPTAEGKAEILTEVSQFMEGDLASKVAHVA
ECCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHE
VGTRPYTPDFAPLIGQLPGFESVFLANGLGASGLTTGPYVGKLLADLALGNTVDLALENY
ECCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECC
EPSKYISR
CCHHHCCC
>Mature Secondary Structure
MQKIVIIGGGIVGASAAYLLSKENVQVTLIDSDEPGQATRAAAGIICPWLSKRRNKYWYE
CCEEEEEECCHHHHHHEEEEECCCEEEEEEECCCCCHHHHHHHHHCCHHHHHHCCHHHHH
LAKNSAAFYKELAGTLEGDTGRNSGYKQVGVLALRKTEEKVTELFNLANERHLDAEVMGE
HHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHHCCCCCCHHHHHH
IAKLSEEETKQKFPLVKPGFGSVYVSGAARVNGSLFCERLLYAAKENGVKIKSGRAHFST
HHHHHHHHHHHHCCCCCCCCCCEEEECCHHCCHHHHHHHHHHHHHCCCEEEECCCEEECC
DGKVRVDGEDEHYDKLIIAAGAWLKELLDEASFHTEVLAQKGQLLELDFSEFQTDEWPVI
CCEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCEE
LPPSAKSIVPFDNGKIIVGATHEKAAGFDTKPTAEGKAEILTEVSQFMEGDLASKVAHVA
ECCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHE
VGTRPYTPDFAPLIGQLPGFESVFLANGLGASGLTTGPYVGKLLADLALGNTVDLALENY
ECCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECC
EPSKYISR
CCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]