Click here to switch to the map view.

The map label for this gene is panB

Identifier: 226224506

GI number: 226224506

Start: 1964172

End: 1965005

Strand: Reverse

Name: panB

Synonym: Lm4b_01919

Alternate gene names: 226224506

Gene position: 1965005-1964172 (Counterclockwise)

Preceding gene: 226224507

Following gene: 226224505

Centisome position: 67.46

GC content: 42.33

Gene sequence:

>834_bases
ATGAAAAGACCAGTAGATTTTTTTGCTATGAAGGAAAACGGAGAGAAAATAACGATGATAACCGCGTATGACTATCCTTC
TGCTAAGAATGTAGAACAAGCAGAAGCTGATATGATTTTAGTCGGCGATTCACTTGGAATGGTAGTATTAGGCTACGATT
CAACTGTACCAGTTACGATGGATGATATGATTCATCATACAAAAGCCGTGAAGCGCGGGGCACCAGATACATTTGTTGTT
ACAGACATGCCGTTTATGACGTATCATGGTTCAGTGGATGAAACTATCCAAAATGCACGGAAGATTATCCAAGAAAGTGG
CGCTCATGCTGTAAAATTAGAAGGAGCAGGAGAAGTCGTTAACAAAATTGCTCGCCTAACAGAAGCAGGTGCTCCAGTTG
TCGCGCATCTTGGCTTAACCCCGCAAAGTGTTGGCTTAACTGGAAGCTATAAAGTACGTGCTAAATCTGCCCAAGAAGCA
CAAGAGCTAATGGATAATGCGTTAGCTGTAGAAGCTGCTGGAGCGATTGCACTCGTACTTGAAGCAATTCCACGTCAACT
CGCTGAAAAAGTAAGTAAAGCACTTTCTATCCCTACAATTGGTATTGGAGCAGGCGTAGAAACAGATGGACAAGTACTCG
TTTATCACGATATTATCGGTTATGGAATCAGTCGTCGCGCCAAATTTGTAAAAGCGTATGCGGACATTGATGAAAGAATC
GAACCAGCACTAGCAAGCTACGTAAAAGAAGTAAAAGCAGCGACGTTTCCAGAAGTGAAACATAGCTTTACCATGGCTGA
AGAAGACTTAAAAGGCCTTTATGGAAGGGAATAG

Upstream 100 bases:

>100_bases
AATGGATGATACCTTTACCGGATCATACTAATGCTACTAAAAAAATATGTTTTTTGCCTTGATTAAAGTAATTTAACAGG
GACGAAAAGGAGCTAAGAAA

Downstream 100 bases:

>100_bases
CAAATGTTAATTATTCGAAATAAACAAGACCTTAAAGAAGCTATTTTAGAGCAAAAAAAAGCGAATAAAACAATTGGTTA
CGTTCCGACGATGGGCTTTT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKRPVDFFAMKENGEKITMITAYDYPSAKNVEQAEADMILVGDSLGMVVLGYDSTVPVTMDDMIHHTKAVKRGAPDTFVV
TDMPFMTYHGSVDETIQNARKIIQESGAHAVKLEGAGEVVNKIARLTEAGAPVVAHLGLTPQSVGLTGSYKVRAKSAQEA
QELMDNALAVEAAGAIALVLEAIPRQLAEKVSKALSIPTIGIGAGVETDGQVLVYHDIIGYGISRRAKFVKAYADIDERI
EPALASYVKEVKAATFPEVKHSFTMAEEDLKGLYGRE

Sequences:

>Translated_277_residues
MKRPVDFFAMKENGEKITMITAYDYPSAKNVEQAEADMILVGDSLGMVVLGYDSTVPVTMDDMIHHTKAVKRGAPDTFVV
TDMPFMTYHGSVDETIQNARKIIQESGAHAVKLEGAGEVVNKIARLTEAGAPVVAHLGLTPQSVGLTGSYKVRAKSAQEA
QELMDNALAVEAAGAIALVLEAIPRQLAEKVSKALSIPTIGIGAGVETDGQVLVYHDIIGYGISRRAKFVKAYADIDERI
EPALASYVKEVKAATFPEVKHSFTMAEEDLKGLYGRE
>Mature_277_residues
MKRPVDFFAMKENGEKITMITAYDYPSAKNVEQAEADMILVGDSLGMVVLGYDSTVPVTMDDMIHHTKAVKRGAPDTFVV
TDMPFMTYHGSVDETIQNARKIIQESGAHAVKLEGAGEVVNKIARLTEAGAPVVAHLGLTPQSVGLTGSYKVRAKSAQEA
QELMDNALAVEAAGAIALVLEAIPRQLAEKVSKALSIPTIGIGAGVETDGQVLVYHDIIGYGISRRAKFVKAYADIDERI
EPALASYVKEVKAATFPEVKHSFTMAEEDLKGLYGRE

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=253, Percent_Identity=44.6640316205534, Blast_Score=219, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6319653, Length=263, Percent_Identity=38.0228136882129, Blast_Score=186, Evalue=5e-48,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 29840; Mature: 29840

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPVDFFAMKENGEKITMITAYDYPSAKNVEQAEADMILVGDSLGMVVLGYDSTVPVTM
CCCCCCEEEEECCCCEEEEEEEECCCCCCCCCHHCCCEEEEECCCCEEEEECCCCCCEEH
DDMIHHTKAVKRGAPDTFVVTDMPFMTYHGSVDETIQNARKIIQESGAHAVKLEGAGEVV
HHHHHHHHHHHCCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCCEEEECCHHHHH
NKIARLTEAGAPVVAHLGLTPQSVGLTGSYKVRAKSAQEAQELMDNALAVEAAGAIALVL
HHHHHHHHCCCCEEEECCCCCHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EAIPRQLAEKVSKALSIPTIGIGAGVETDGQVLVYHDIIGYGISRRAKFVKAYADIDERI
HHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHH
EPALASYVKEVKAATFPEVKHSFTMAEEDLKGLYGRE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRPVDFFAMKENGEKITMITAYDYPSAKNVEQAEADMILVGDSLGMVVLGYDSTVPVTM
CCCCCCEEEEECCCCEEEEEEEECCCCCCCCCHHCCCEEEEECCCCEEEEECCCCCCEEH
DDMIHHTKAVKRGAPDTFVVTDMPFMTYHGSVDETIQNARKIIQESGAHAVKLEGAGEVV
HHHHHHHHHHHCCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCCEEEECCHHHHH
NKIARLTEAGAPVVAHLGLTPQSVGLTGSYKVRAKSAQEAQELMDNALAVEAAGAIALVL
HHHHHHHHCCCCEEEECCCCCHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EAIPRQLAEKVSKALSIPTIGIGAGVETDGQVLVYHDIIGYGISRRAKFVKAYADIDERI
HHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHH
EPALASYVKEVKAATFPEVKHSFTMAEEDLKGLYGRE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]