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The map label for this gene is ypcP [H]

Identifier: 226224484

GI number: 226224484

Start: 1942120

End: 1942992

Strand: Reverse

Name: ypcP [H]

Synonym: Lm4b_01897

Alternate gene names: 226224484

Gene position: 1942992-1942120 (Counterclockwise)

Preceding gene: 226224485

Following gene: 226224481

Centisome position: 66.71

GC content: 40.21

Gene sequence:

>873_bases
ATGACCGAAAATCGAGAAAATTTGCTCGTTGTTGATGGAATGGCACTACTTTTCCGAGCATTTTATGCAACAGCCGTTTC
CAAACAATTTATGTTTAACCAACATGGAATCCCGACGAATGGGGTGCAAGGTTTTATGCGCCACATGTTTGCAGCGATTC
GCCAGAGTAACCCAACGCATACACTAATTTGCTGGGATATGGGATCGCAAACATTTCGAAATGAATTATATGATGGTTAT
AAAGCAGGCAGAACAGCGCCACCAGAAGAAATGATTCCGCAGTTTGATTTAGCGAAAGAGGTAGCTGCTGGATTTGGCTT
TGTCAATTTAGGTGTGCCGGGTTTTGAAGCAGATGATTGTATTGGGACAATTACCGTTCAAGCGAGTAATACGATTGCTA
CAACTGTTTTAAGTGGCGACAAAGATTTACTGCAACTAATCGCACCGACGAATGATGTCTGGATTATGCAAAAAGGTTAT
GGCAATTATAAACGATATGATGAAGCGACATTTTTTGAAGAGATGGGTATTTCTCCAAGACAATTTATTGATGTGAAAGC
ATTAATGGGCGATACTTCAGATGGTTATCCGGGTGTTCGTGGAATCGGTGAAAAAACAGCGATTAAGCTTATTCAAGAAT
TTGAGTCTATTGAAGGCGTCTTGAACAACCTTGATAAACTGAAACCAGCGCAACAAACCAAAATCCAAGAAGACTTAGCG
ATGCTAGAATTAAGTCAAAAATTAGCTCGGATTCATACAGAAGTCCCACTAGAAATAGACTTGCCTAGCTTGAAATATGA
CGGTTTCCGCGAGGATGCTTTTGCTGTAGTTGAAAAATACGGTTTAAAAACGTTAACGCGCGATATCGAATAA

Upstream 100 bases:

>100_bases
GCGTGACAAAAGCAAGCTGGTAAAAAGCTCCTTTTTTTCTGCGAGCCAGTTATAATTATGCTACAATAGATGGCAGTAGA
AGGAAATTGGAGGATTATAT

Downstream 100 bases:

>100_bases
AAAATAGAAGCAATCTGGTTATTTGACTCCAGGTTGCTTCTGTTTTTTTATAGCCTGGCGCGCAAGTTGGTCGGCTTGTT
TATTTTGACTTACATTACGC

Product: 5'-3' exonuclease

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTENRENLLVVDGMALLFRAFYATAVSKQFMFNQHGIPTNGVQGFMRHMFAAIRQSNPTHTLICWDMGSQTFRNELYDGY
KAGRTAPPEEMIPQFDLAKEVAAGFGFVNLGVPGFEADDCIGTITVQASNTIATTVLSGDKDLLQLIAPTNDVWIMQKGY
GNYKRYDEATFFEEMGISPRQFIDVKALMGDTSDGYPGVRGIGEKTAIKLIQEFESIEGVLNNLDKLKPAQQTKIQEDLA
MLELSQKLARIHTEVPLEIDLPSLKYDGFREDAFAVVEKYGLKTLTRDIE

Sequences:

>Translated_290_residues
MTENRENLLVVDGMALLFRAFYATAVSKQFMFNQHGIPTNGVQGFMRHMFAAIRQSNPTHTLICWDMGSQTFRNELYDGY
KAGRTAPPEEMIPQFDLAKEVAAGFGFVNLGVPGFEADDCIGTITVQASNTIATTVLSGDKDLLQLIAPTNDVWIMQKGY
GNYKRYDEATFFEEMGISPRQFIDVKALMGDTSDGYPGVRGIGEKTAIKLIQEFESIEGVLNNLDKLKPAQQTKIQEDLA
MLELSQKLARIHTEVPLEIDLPSLKYDGFREDAFAVVEKYGLKTLTRDIE
>Mature_289_residues
TENRENLLVVDGMALLFRAFYATAVSKQFMFNQHGIPTNGVQGFMRHMFAAIRQSNPTHTLICWDMGSQTFRNELYDGYK
AGRTAPPEEMIPQFDLAKEVAAGFGFVNLGVPGFEADDCIGTITVQASNTIATTVLSGDKDLLQLIAPTNDVWIMQKGYG
NYKRYDEATFFEEMGISPRQFIDVKALMGDTSDGYPGVRGIGEKTAIKLIQEFESIEGVLNNLDKLKPAQQTKIQEDLAM
LELSQKLARIHTEVPLEIDLPSLKYDGFREDAFAVVEKYGLKTLTRDIE

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=300, Percent_Identity=31, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI226510970, Length=263, Percent_Identity=30.4182509505703, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 32414; Mature: 32283

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENRENLLVVDGMALLFRAFYATAVSKQFMFNQHGIPTNGVQGFMRHMFAAIRQSNPTH
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCE
TLICWDMGSQTFRNELYDGYKAGRTAPPEEMIPQFDLAKEVAAGFGFVNLGVPGFEADDC
EEEEEECCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCEEECCCCCCCCCCC
IGTITVQASNTIATTVLSGDKDLLQLIAPTNDVWIMQKGYGNYKRYDEATFFEEMGISPR
EEEEEEEECCEEEEEEECCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHH
QFIDVKALMGDTSDGYPGVRGIGEKTAIKLIQEFESIEGVLNNLDKLKPAQQTKIQEDLA
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
MLELSQKLARIHTEVPLEIDLPSLKYDGFREDAFAVVEKYGLKTLTRDIE
HHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TENRENLLVVDGMALLFRAFYATAVSKQFMFNQHGIPTNGVQGFMRHMFAAIRQSNPTH
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCE
TLICWDMGSQTFRNELYDGYKAGRTAPPEEMIPQFDLAKEVAAGFGFVNLGVPGFEADDC
EEEEEECCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCEEECCCCCCCCCCC
IGTITVQASNTIATTVLSGDKDLLQLIAPTNDVWIMQKGYGNYKRYDEATFFEEMGISPR
EEEEEEEECCEEEEEEECCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHH
QFIDVKALMGDTSDGYPGVRGIGEKTAIKLIQEFESIEGVLNNLDKLKPAQQTKIQEDLA
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
MLELSQKLARIHTEVPLEIDLPSLKYDGFREDAFAVVEKYGLKTLTRDIE
HHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]