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The map label for this gene is pepT [H]

Identifier: 226224383

GI number: 226224383

Start: 1842390

End: 1843622

Strand: Reverse

Name: pepT [H]

Synonym: Lm4b_01796

Alternate gene names: 226224383

Gene position: 1843622-1842390 (Counterclockwise)

Preceding gene: 226224384

Following gene: 226224380

Centisome position: 63.3

GC content: 38.12

Gene sequence:

>1233_bases
ATGAAAGAAGAACTATTAAAACGATTTACGAAATATGTAAAAGTAGATACACAGTCAAATGAAGAAAGTAAAGCTTGTCC
AACAACACCAGGTCAAATGGAACTGGCAAACATTCTCGTTACAGAACTAAAAGAAATCGGTATGCAAGAAGTTACTGTTG
ACGAATTTGGTTATGTTATGGCAACACTTCCTTCTAATACAACGAAAGAAGTTCCAGTAATCGGATTTTTAGCTCATTTA
GATACAGCGACGGACTTAACAGGCAAAAACGTCCAACCACAAGTCCACGAAAATTATGATGGTAAAGATATCGTTCTAAA
CAAAGACTTAAACGTGGTACTTTCACCAAAACAATTTCCAGAACTAGCTGATTATAATGGGAAAACACTGATTACAACAG
ATGGAACAACGCTACTCGGTGCAGACGATAAAGCGGGCATTACCGAAATTATGGTGGCAATGAACCATTTAATTAACCAT
CCCGAAATCAAACACGGTAAAATTCGTGTTGCTTTCACACCAGATGAAGAAATTGGCCGAGGACCAGAACGTTTTGATGT
AGAAGCATTTGGCGCAAAATATGCTTATACAATGGACGGGGGCCCACTTGGAGAACTAGAATATGAAAGTTTTAACGCTG
CAGGTGCCAAAATCACTTTTAATGGTAATAGTGTTCACCCTGGTACAGCAAAAAACAAAATGGTTAATGCTGTCAAAATG
GCAATGGAATTCAATGCACACATTCCAAAAGACGAAGCGCCTGAGTACACAGAAGGTTACGAAGGGTTTTATCATTTAAT
TTCCTTAAACGGTGATGTAGAGCAAGCCAAAGCCTACTATATTATCCGCGATTTCGATCATTTGAAATTTGTGGAAAGAA
AAACACATATTGCTAGTATCGCCAAAGAGTTAGAAGAAAAATACGGCGAAGGATCGGTAGAATTAAAACTGAACGATCAA
TATTACAATATGCGCGAAAAAATCGAACCAGTAAAAGAAATTGTGGATATCGTCAGCGCGGCGATGAGAAATCTAGACAT
TGAACCGAAAATCAGCCCAATTCGTGGCGGAACAGACGGCGCTCAACTTTCCTACAAAGGACTACCAACACCTAATATTT
TTGGTGGAGGAGAGAACTTCCACGGTAAATTTGAATATGTTGCATTAGAAAGTATGGTTAAAGCAACAGAAGTAATCATT
GAAGTGGCTCGTTTATTTGAAGAAAAAGAATAA

Upstream 100 bases:

>100_bases
AACAACCCTCCAATTACAAACACTACTCGACAGTCAGGAATAGAATTTTTCGAAAATCAAGTTTATAATGAAAAAGAATC
AATTTAAGGGAGTGGTCGGA

Downstream 100 bases:

>100_bases
TAAAAAAGTGAGTCTAGTTCATCCTAGACTCACTTTTTCTATGCGCTCATTTACTCTAGCAGTAAAGACCTTTGCTTCGC
CGTTTGCAACAAGTAACGTA

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKEELLKRFTKYVKVDTQSNEESKACPTTPGQMELANILVTELKEIGMQEVTVDEFGYVMATLPSNTTKEVPVIGFLAHL
DTATDLTGKNVQPQVHENYDGKDIVLNKDLNVVLSPKQFPELADYNGKTLITTDGTTLLGADDKAGITEIMVAMNHLINH
PEIKHGKIRVAFTPDEEIGRGPERFDVEAFGAKYAYTMDGGPLGELEYESFNAAGAKITFNGNSVHPGTAKNKMVNAVKM
AMEFNAHIPKDEAPEYTEGYEGFYHLISLNGDVEQAKAYYIIRDFDHLKFVERKTHIASIAKELEEKYGEGSVELKLNDQ
YYNMREKIEPVKEIVDIVSAAMRNLDIEPKISPIRGGTDGAQLSYKGLPTPNIFGGGENFHGKFEYVALESMVKATEVII
EVARLFEEKE

Sequences:

>Translated_410_residues
MKEELLKRFTKYVKVDTQSNEESKACPTTPGQMELANILVTELKEIGMQEVTVDEFGYVMATLPSNTTKEVPVIGFLAHL
DTATDLTGKNVQPQVHENYDGKDIVLNKDLNVVLSPKQFPELADYNGKTLITTDGTTLLGADDKAGITEIMVAMNHLINH
PEIKHGKIRVAFTPDEEIGRGPERFDVEAFGAKYAYTMDGGPLGELEYESFNAAGAKITFNGNSVHPGTAKNKMVNAVKM
AMEFNAHIPKDEAPEYTEGYEGFYHLISLNGDVEQAKAYYIIRDFDHLKFVERKTHIASIAKELEEKYGEGSVELKLNDQ
YYNMREKIEPVKEIVDIVSAAMRNLDIEPKISPIRGGTDGAQLSYKGLPTPNIFGGGENFHGKFEYVALESMVKATEVII
EVARLFEEKE
>Mature_410_residues
MKEELLKRFTKYVKVDTQSNEESKACPTTPGQMELANILVTELKEIGMQEVTVDEFGYVMATLPSNTTKEVPVIGFLAHL
DTATDLTGKNVQPQVHENYDGKDIVLNKDLNVVLSPKQFPELADYNGKTLITTDGTTLLGADDKAGITEIMVAMNHLINH
PEIKHGKIRVAFTPDEEIGRGPERFDVEAFGAKYAYTMDGGPLGELEYESFNAAGAKITFNGNSVHPGTAKNKMVNAVKM
AMEFNAHIPKDEAPEYTEGYEGFYHLISLNGDVEQAKAYYIIRDFDHLKFVERKTHIASIAKELEEKYGEGSVELKLNDQ
YYNMREKIEPVKEIVDIVSAAMRNLDIEPKISPIRGGTDGAQLSYKGLPTPNIFGGGENFHGKFEYVALESMVKATEVII
EVARLFEEKE

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=409, Percent_Identity=53.7897310513447, Blast_Score=445, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 45655; Mature: 45655

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEELLKRFTKYVKVDTQSNEESKACPTTPGQMELANILVTELKEIGMQEVTVDEFGYVM
CHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHCEEE
ATLPSNTTKEVPVIGFLAHLDTATDLTGKNVQPQVHENYDGKDIVLNKDLNVVLSPKQFP
EECCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCHHCCCCCCCEEEEECCCCEEECCCCCC
ELADYNGKTLITTDGTTLLGADDKAGITEIMVAMNHLINHPEIKHGKIRVAFTPDEEIGR
HHHCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCC
GPERFDVEAFGAKYAYTMDGGPLGELEYESFNAAGAKITFNGNSVHPGTAKNKMVNAVKM
CCCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHH
AMEFNAHIPKDEAPEYTEGYEGFYHLISLNGDVEQAKAYYIIRDFDHLKFVERKTHIASI
HHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCEEEEEECCHHHHHHHHHHHHHHH
AKELEEKYGEGSVELKLNDQYYNMREKIEPVKEIVDIVSAAMRNLDIEPKISPIRGGTDG
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AQLSYKGLPTPNIFGGGENFHGKFEYVALESMVKATEVIIEVARLFEEKE
CEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEELLKRFTKYVKVDTQSNEESKACPTTPGQMELANILVTELKEIGMQEVTVDEFGYVM
CHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHCEEE
ATLPSNTTKEVPVIGFLAHLDTATDLTGKNVQPQVHENYDGKDIVLNKDLNVVLSPKQFP
EECCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCHHCCCCCCCEEEEECCCCEEECCCCCC
ELADYNGKTLITTDGTTLLGADDKAGITEIMVAMNHLINHPEIKHGKIRVAFTPDEEIGR
HHHCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCC
GPERFDVEAFGAKYAYTMDGGPLGELEYESFNAAGAKITFNGNSVHPGTAKNKMVNAVKM
CCCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHH
AMEFNAHIPKDEAPEYTEGYEGFYHLISLNGDVEQAKAYYIIRDFDHLKFVERKTHIASI
HHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCEEEEEECCHHHHHHHHHHHHHHH
AKELEEKYGEGSVELKLNDQYYNMREKIEPVKEIVDIVSAAMRNLDIEPKISPIRGGTDG
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AQLSYKGLPTPNIFGGGENFHGKFEYVALESMVKATEVIIEVARLFEEKE
CEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]