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The map label for this gene is gltD

Identifier: 226224336

GI number: 226224336

Start: 1785201

End: 1786670

Strand: Reverse

Name: gltD

Synonym: Lm4b_01747

Alternate gene names: 226224336

Gene position: 1786670-1785201 (Counterclockwise)

Preceding gene: 226224337

Following gene: 226224332

Centisome position: 61.34

GC content: 44.08

Gene sequence:

>1470_bases
ATGGGAAAAGCAACTGGATTTATGGAATATGACCGAATTCCCTCTCCAGGACGTGATCCGAAAAGCCGGACACGTGACTG
GAACGAATATAGTTTACCAATGCCGGTAGCAGATTTAACATTACAAGCCGCTCGTTGCATGGATTGTGGCGTACCGTTTT
GTAGTGTAGGAATGGAAATTAAAAATGGTGTTTCAGGCTGTCCGCTGCATAATTTAATTCCCGAGTGGAACGATGCCGTT
TATCGGGGTGACTGGTATGAAGCCTTACAACTACTCTTAAAAACAAATAATTTTCCAGAGTTTACTGGTCGTATTTGTCC
GGCACCGTGTGAAGGTGGCTGTACGGTGGCAATTTCGGAACCTGCTGTAGGGATTAAATCCATCGAAAAAGCGATTATTG
ACCGAGGTTTTGAAGAAGGCTGGATTAAACCGGCTCCTCCTAAAAAACGTACCGGTAAACGAATTGCTGTGATTGGGTCA
GGACCGGCTGGACTTGCCTGCGCCGATCAATTAAATAAAGCTGGCCATAGCGTAACGGTCATTGAAAAAAGTGATCGCAT
TGGTGGCTTATTAATGTACGGTATTCCAACGATGAAACTGGAGAAAGAACAAGTAACGCGCCGAGTGAACTTGATGGCGG
AAGAAGGAATCGAATTCGTCACAGGTGTTGCAGCAGGGACAGATGTTACTTTTGCAGAACTACGAGAAGAGTATGATTCT
ATCGTTCTTGCGACAGGAGCAGGGAATGCGCGCGATATCCCACTTGCTGGACGTGATGCAGTTGGGATTCACTTCGCTGT
TCCGTATTTATCGCAAAGTACGCGTGATAATCTGGATAACGGCGGGGTACAAACACTTTCTGCTAAAGGTAAAAATGTGG
TGATTATCGGTGGTGGAGATACAGGTGCTGACTGTGTGGCAACTGCGCTCAGACAAGGGGCAAAAAGTATTTATCAATTC
GGTATTCAAGATAAACTACCCGAATTGCGTGATGGCGAAAACCCTTGGCCGCAATATCCACGTGTTTTCAAAATGGATTA
TGCGCACGAAGAAGCGGAAGCAGTGTATGGAAGAGATCCACGTGAGTACCTTATTAATACAACTTCTTTTGAAAAAGATG
AAGCAGGAAATCTTATTGGACTGCATACTGTAGAAGTAGAAGAAAATAAGGCAGAAAGAAAATATACACCTGTAGAAGGT
AGCGAAAAATACTTTGAAGTAGATATGGTTCTAATTGCGATTGGTTTTGCAGGAACAACCGAAGATATTTTTACAAACTT
TGGTGTAGGAAAAACAGAGCGCCATACGATAGATGCTGCTAAAGGATTTTACCGCACAAGCGAAGAGGGCGTATTCGCTT
GCGGAGATGCGCGTCACGGTCAAAGTTTAGTTGTAACCGCGATTGCAGAAGGACGAGAAGCAGCTCGTGAAGTAGACTTT
TACTTGATGGGTGAAACATTTTTACCATAA

Upstream 100 bases:

>100_bases
AAACTGGAAAAACACATGATGAAGCTGAATTACAAGCTTTTTATGAACATAAAGACGGAAAATTAGTTGCCGGAGTGGCG
AAATAGAGAGGAGTGAAACC

Downstream 100 bases:

>100_bases
TAAAAAAGAGCTTATCCTAAATGGGTAAGCTCTTTTTTGACTAATTATTTCATTCCAGAAACGTTGAAGCCTTTTAAAAT
GTACTTTTGGAATGTCATGA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MGKATGFMEYDRIPSPGRDPKSRTRDWNEYSLPMPVADLTLQAARCMDCGVPFCSVGMEIKNGVSGCPLHNLIPEWNDAV
YRGDWYEALQLLLKTNNFPEFTGRICPAPCEGGCTVAISEPAVGIKSIEKAIIDRGFEEGWIKPAPPKKRTGKRIAVIGS
GPAGLACADQLNKAGHSVTVIEKSDRIGGLLMYGIPTMKLEKEQVTRRVNLMAEEGIEFVTGVAAGTDVTFAELREEYDS
IVLATGAGNARDIPLAGRDAVGIHFAVPYLSQSTRDNLDNGGVQTLSAKGKNVVIIGGGDTGADCVATALRQGAKSIYQF
GIQDKLPELRDGENPWPQYPRVFKMDYAHEEAEAVYGRDPREYLINTTSFEKDEAGNLIGLHTVEVEENKAERKYTPVEG
SEKYFEVDMVLIAIGFAGTTEDIFTNFGVGKTERHTIDAAKGFYRTSEEGVFACGDARHGQSLVVTAIAEGREAAREVDF
YLMGETFLP

Sequences:

>Translated_489_residues
MGKATGFMEYDRIPSPGRDPKSRTRDWNEYSLPMPVADLTLQAARCMDCGVPFCSVGMEIKNGVSGCPLHNLIPEWNDAV
YRGDWYEALQLLLKTNNFPEFTGRICPAPCEGGCTVAISEPAVGIKSIEKAIIDRGFEEGWIKPAPPKKRTGKRIAVIGS
GPAGLACADQLNKAGHSVTVIEKSDRIGGLLMYGIPTMKLEKEQVTRRVNLMAEEGIEFVTGVAAGTDVTFAELREEYDS
IVLATGAGNARDIPLAGRDAVGIHFAVPYLSQSTRDNLDNGGVQTLSAKGKNVVIIGGGDTGADCVATALRQGAKSIYQF
GIQDKLPELRDGENPWPQYPRVFKMDYAHEEAEAVYGRDPREYLINTTSFEKDEAGNLIGLHTVEVEENKAERKYTPVEG
SEKYFEVDMVLIAIGFAGTTEDIFTNFGVGKTERHTIDAAKGFYRTSEEGVFACGDARHGQSLVVTAIAEGREAAREVDF
YLMGETFLP
>Mature_488_residues
GKATGFMEYDRIPSPGRDPKSRTRDWNEYSLPMPVADLTLQAARCMDCGVPFCSVGMEIKNGVSGCPLHNLIPEWNDAVY
RGDWYEALQLLLKTNNFPEFTGRICPAPCEGGCTVAISEPAVGIKSIEKAIIDRGFEEGWIKPAPPKKRTGKRIAVIGSG
PAGLACADQLNKAGHSVTVIEKSDRIGGLLMYGIPTMKLEKEQVTRRVNLMAEEGIEFVTGVAAGTDVTFAELREEYDSI
VLATGAGNARDIPLAGRDAVGIHFAVPYLSQSTRDNLDNGGVQTLSAKGKNVVIIGGGDTGADCVATALRQGAKSIYQFG
IQDKLPELRDGENPWPQYPRVFKMDYAHEEAEAVYGRDPREYLINTTSFEKDEAGNLIGLHTVEVEENKAERKYTPVEGS
EKYFEVDMVLIAIGFAGTTEDIFTNFGVGKTERHTIDAAKGFYRTSEEGVFACGDARHGQSLVVTAIAEGREAAREVDFY
LMGETFLP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=478, Percent_Identity=22.8033472803347, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1789606, Length=488, Percent_Identity=36.2704918032787, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1788811, Length=477, Percent_Identity=34.5911949685535, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI87082180, Length=451, Percent_Identity=32.5942350332594, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1789243, Length=437, Percent_Identity=28.3752860411899, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788468, Length=445, Percent_Identity=25.6179775280899, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17570289, Length=497, Percent_Identity=48.0885311871227, Blast_Score=457, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71984108, Length=464, Percent_Identity=22.6293103448276, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17543860, Length=163, Percent_Identity=33.7423312883436, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=494, Percent_Identity=47.5708502024291, Blast_Score=472, Evalue=1e-134,
Organism=Drosophila melanogaster, GI28574881, Length=488, Percent_Identity=54.9180327868852, Blast_Score=544, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24665539, Length=488, Percent_Identity=54.9180327868852, Blast_Score=544, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24665547, Length=488, Percent_Identity=54.9180327868852, Blast_Score=542, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24665543, Length=488, Percent_Identity=54.9180327868852, Blast_Score=542, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24640763, Length=458, Percent_Identity=22.707423580786, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18858217, Length=458, Percent_Identity=22.707423580786, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53272; Mature: 53141

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFMEYDRIPSPGRDPKSRTRDWNEYSLPMPVADLTLQAARCMDCGVPFCSVGMEI
CCCCCCCEECCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCHHH
KNGVSGCPLHNLIPEWNDAVYRGDWYEALQLLLKTNNFPEFTGRICPAPCEGGCTVAISE
HCCCCCCCHHHCCCCCCCCEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECC
PAVGIKSIEKAIIDRGFEEGWIKPAPPKKRTGKRIAVIGSGPAGLACADQLNKAGHSVTV
CCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
IEKSDRIGGLLMYGIPTMKLEKEQVTRRVNLMAEEGIEFVTGVAAGTDVTFAELREEYDS
EECCCCCCCEEEECCCCCEECHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHCC
IVLATGAGNARDIPLAGRDAVGIHFAVPYLSQSTRDNLDNGGVQTLSAKGKNVVIIGGGD
EEEEECCCCCCCCCCCCCCCCEEEEEECHHCCHHHCCCCCCCEEEEECCCCEEEEEECCC
TGADCVATALRQGAKSIYQFGIQDKLPELRDGENPWPQYPRVFKMDYAHEEAEAVYGRDP
CCHHHHHHHHHHHHHHHHHHCCHHCCCHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCH
REYLINTTSFEKDEAGNLIGLHTVEVEENKAERKYTPVEGSEKYFEVDMVLIAIGFAGTT
HHHEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCH
EDIFTNFGVGKTERHTIDAAKGFYRTSEEGVFACGDARHGQSLVVTAIAEGREAAREVDF
HHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEHHHHHHHHHCCE
YLMGETFLP
EEECCCCCC
>Mature Secondary Structure 
GKATGFMEYDRIPSPGRDPKSRTRDWNEYSLPMPVADLTLQAARCMDCGVPFCSVGMEI
CCCCCCEECCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCHHH
KNGVSGCPLHNLIPEWNDAVYRGDWYEALQLLLKTNNFPEFTGRICPAPCEGGCTVAISE
HCCCCCCCHHHCCCCCCCCEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECC
PAVGIKSIEKAIIDRGFEEGWIKPAPPKKRTGKRIAVIGSGPAGLACADQLNKAGHSVTV
CCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
IEKSDRIGGLLMYGIPTMKLEKEQVTRRVNLMAEEGIEFVTGVAAGTDVTFAELREEYDS
EECCCCCCCEEEECCCCCEECHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHCC
IVLATGAGNARDIPLAGRDAVGIHFAVPYLSQSTRDNLDNGGVQTLSAKGKNVVIIGGGD
EEEEECCCCCCCCCCCCCCCCEEEEEECHHCCHHHCCCCCCCEEEEECCCCEEEEEECCC
TGADCVATALRQGAKSIYQFGIQDKLPELRDGENPWPQYPRVFKMDYAHEEAEAVYGRDP
CCHHHHHHHHHHHHHHHHHHCCHHCCCHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCH
REYLINTTSFEKDEAGNLIGLHTVEVEENKAERKYTPVEGSEKYFEVDMVLIAIGFAGTT
HHHEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCH
EDIFTNFGVGKTERHTIDAAKGFYRTSEEGVFACGDARHGQSLVVTAIAEGREAAREVDF
HHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEHHHHHHHHHCCE
YLMGETFLP
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]