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The map label for this gene is adhE [H]

Identifier: 226224235

GI number: 226224235

Start: 1661122

End: 1663722

Strand: Direct

Name: adhE [H]

Synonym: Lm4b_01645

Alternate gene names: 226224235

Gene position: 1661122-1663722 (Clockwise)

Preceding gene: 226224223

Following gene: 226224236

Centisome position: 57.03

GC content: 39.68

Gene sequence:

>2601_bases
ATGGCAATTAAAGAAAATGCGGCCCAAGAAGTATTAGAAGTTCAAAAAGTGATTGACAGATTAGCAGACAATGGACAAAA
AGCATTGAAAGCATTTGAAAGTTACAATCAAGAACAAGTAGACAATATCGTTCACGCAATGGCACTTGCCGGACTTGACC
AACATATGCCCCTTGCAAAATTAGCAGTAGAAGAAACTGGACGTGGATTATACGAAGATAAATGTATTAAAAACATCTTC
GCGACAGAATATATTTGGAACAACATTAAAAACAACAAAACAGTAGGCGTTATTAATGAAGATGTACAAACTGGTGTGAT
TGAAATTGCTGAACCAGTTGGAGTTGTTGCCGGGGTTACACCTGTAACTAACCCAACATCCACTACTCTTTTCAAAGCAA
TTATCGCTATCAAAACACGTAACCCAATCATCTTTGCTTTCCATCCAAGTGCACAGCGTTGTTCATCTGCAGCAGCGAAA
GTTGTATACGATGCAGCAATTGCAGCTGGAGCACCAGAACATTGTATTCAATGGGTAGAAAAACCTTCCCTAGAAGCAAC
GAAACAATTAATGAATCACGATAAAGTAGCACTTGTACTTGCAACTGGTGGTGCTGGAATGGTTAAATCAGCATACTCTA
CTGGTAAACCTGCACTAGGTGTTGGACCAGGTAACGTACCAGCTTACATTGACAAAACAGCCAAAATTAAACGTTCTGTT
AATGACATTATTCTTTCTAAATCTTTTGACCAAGGTATGATTTGTGCTTCTGAACAAGCAGTCATCGTGGATAAAGAAGT
CGCTAAAGAAGTTAAAGCTGAAATGGAAGCAAACAAATGCTACTTCGTTAAAGGCGCTGAATTCAAAAAATTAGAAAGCT
ACGTAATCAATCCTGAAAAAGGAACACTTAACCCAGATGTAGTTGGTAAATCCCCTGCATGGATTGCAAACCAAGCTGGC
TTCAAAGTTCCAGAAGATACAAAAATTCTTGTAGCTGAAATTAAAGGTGTTGGCGACAAATACCCACTATCTCACGAAAA
ATTAAGCCCAGTTCTTGCATTCATCGAAGCTGCTAACCAAGCAGAAGCATTCGATCGTTGTGAAGAAATGTTAGTATACG
GAGGACTTGGACACTCCGCAGTTATTCACTCTACGGATAAAGAAGTTCAAAAAGCATTTGGTATTCGTATGAAAGCTTGC
CGTATCATCGTAAATGCACCAAGCGCACAAGGCGGTATCGGTGACATTTATAACGGCTTCATCCCTTCCCTAACACTAGG
TTGTGGATCTTACGGTAAAAACTCTGTATCACAAAATGTAAGTGCGACTAACTTGCTGAACGTTAAACGTATCGCGGATC
GGAGAAATAATATGCAATGGTTCAAACTTCCACCAAAAATTTTCTTTGAAAAATATTCCACTCAATACCTTCAAAAAATG
GAAGGCGTTGAACGCGTATTTATCGTAACTGACCCAGGAATGGTTCAATTCAAATACGTGGATGTCGTAATCGAACACCT
GAAAAAACGTGGCAACGATGTAGCTTACCAAGTATTTGCTGACGTTGAACCAGATCCATCTGACGTAACAGTTTACAAAG
GTGCAGAACTAATGAAAGACTTCAAACCTGATACAATTATCGCTCTTGGTGGTGGTTCCGCAATGGATGCTGCCAAAGGT
ATGTGGTTATTCTATGAACACCCAGAAGCTTCATTCTTCGGATTGAAACAAAAATTCTTAGATATCCGTAAACGTACTTT
CAAATATCCTAAACTTGGTGGAAAAGCGAAATTCGTTGCAATTCCAACAACAAGTGGTACAGGTTCTGAAGTAACTCCAT
TTGCGGTTATTACAGACAAAGAAAACAACATTAAATACCCTCTTGCTGATTATGAACTAACTCCAGACGTTGCGATTGTT
GATGCACAATATGTAACTACTGTTCCAGCACACATTACTGCTGACACTGGTATGGACGTTCTAACTCACGCAATTGAATC
TTATGTATCCGTAATGGCAAGCGATTATACTCGTGGATTATCTATCCGCGCAATCGAACTTGTATTTGAAAACTTACGTG
AATCTGTTCTTACTGGTGATCCAGATGCGCGTGAAAAAATGCATAATGCTTCTGCCCTAGCTGGTATGGCGTTTGCAAAT
GCGTTCCTAGGAATTAACCACAGCTTGGCACACAAAATTGGACCTGAATTCCACATTCCTCACGGTCGTGCGAATGCAAT
CCTTATGCCACATGTTATTCGTTATAACGCACTTAAACCTAAAAAACATGCGTTATTCCCTAGATATGAAAGCTTCCGTG
CTGATGAAGATTATGCTCGTATCTCTCGTATTATCGGCTTCCCTGCAGCAACTACAGAAGAAGGCGTTAAATCACTTGTA
GATGAAATCATCAAACTTGGTAAAGACGTTGGTATCGACATGAGTCTTAAAGGACAAAATGTTGCTAAAAAAGACTTGGA
TGCAGTTGTAGATACACTTGCAGATCGCGCATTCATGGACCAATGTACTACTGCCAACCCTAAACAACCACTTGTAAGTG
AACTCAAAGAAATCTACCTAGAAGCTTACAAAGGTGTTTGA

Upstream 100 bases:

>100_bases
ACGGCAAAGCAACAAGCTAAATTTTTTTGGATTATCAGGGACGTTAAAAGTCTACTATGGACATTTTAAGTCCCGAACAA
ATATTAGGAGGTTCTGGAAA

Downstream 100 bases:

>100_bases
AACTAGCTAACTTGTCCACCATTAATATATAAAGTTAGAAGAAAGCGCAAGTCCATCCCAGGGCTTGCGCTTTCGCTTTA
GTACTAAAATTAGTATAATG

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 866; Mature: 865

Protein sequence:

>866_residues
MAIKENAAQEVLEVQKVIDRLADNGQKALKAFESYNQEQVDNIVHAMALAGLDQHMPLAKLAVEETGRGLYEDKCIKNIF
ATEYIWNNIKNNKTVGVINEDVQTGVIEIAEPVGVVAGVTPVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSSAAAK
VVYDAAIAAGAPEHCIQWVEKPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKRSV
NDIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINPEKGTLNPDVVGKSPAWIANQAG
FKVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAANQAEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKAC
RIIVNAPSAQGGIGDIYNGFIPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADRRNNMQWFKLPPKIFFEKYSTQYLQKM
EGVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAYQVFADVEPDPSDVTVYKGAELMKDFKPDTIIALGGGSAMDAAKG
MWLFYEHPEASFFGLKQKFLDIRKRTFKYPKLGGKAKFVAIPTTSGTGSEVTPFAVITDKENNIKYPLADYELTPDVAIV
DAQYVTTVPAHITADTGMDVLTHAIESYVSVMASDYTRGLSIRAIELVFENLRESVLTGDPDAREKMHNASALAGMAFAN
AFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNALKPKKHALFPRYESFRADEDYARISRIIGFPAATTEEGVKSLV
DEIIKLGKDVGIDMSLKGQNVAKKDLDAVVDTLADRAFMDQCTTANPKQPLVSELKEIYLEAYKGV

Sequences:

>Translated_866_residues
MAIKENAAQEVLEVQKVIDRLADNGQKALKAFESYNQEQVDNIVHAMALAGLDQHMPLAKLAVEETGRGLYEDKCIKNIF
ATEYIWNNIKNNKTVGVINEDVQTGVIEIAEPVGVVAGVTPVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSSAAAK
VVYDAAIAAGAPEHCIQWVEKPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKRSV
NDIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINPEKGTLNPDVVGKSPAWIANQAG
FKVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAANQAEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKAC
RIIVNAPSAQGGIGDIYNGFIPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADRRNNMQWFKLPPKIFFEKYSTQYLQKM
EGVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAYQVFADVEPDPSDVTVYKGAELMKDFKPDTIIALGGGSAMDAAKG
MWLFYEHPEASFFGLKQKFLDIRKRTFKYPKLGGKAKFVAIPTTSGTGSEVTPFAVITDKENNIKYPLADYELTPDVAIV
DAQYVTTVPAHITADTGMDVLTHAIESYVSVMASDYTRGLSIRAIELVFENLRESVLTGDPDAREKMHNASALAGMAFAN
AFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNALKPKKHALFPRYESFRADEDYARISRIIGFPAATTEEGVKSLV
DEIIKLGKDVGIDMSLKGQNVAKKDLDAVVDTLADRAFMDQCTTANPKQPLVSELKEIYLEAYKGV
>Mature_865_residues
AIKENAAQEVLEVQKVIDRLADNGQKALKAFESYNQEQVDNIVHAMALAGLDQHMPLAKLAVEETGRGLYEDKCIKNIFA
TEYIWNNIKNNKTVGVINEDVQTGVIEIAEPVGVVAGVTPVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSSAAAKV
VYDAAIAAGAPEHCIQWVEKPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKRSVN
DIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINPEKGTLNPDVVGKSPAWIANQAGF
KVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAANQAEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKACR
IIVNAPSAQGGIGDIYNGFIPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADRRNNMQWFKLPPKIFFEKYSTQYLQKME
GVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAYQVFADVEPDPSDVTVYKGAELMKDFKPDTIIALGGGSAMDAAKGM
WLFYEHPEASFFGLKQKFLDIRKRTFKYPKLGGKAKFVAIPTTSGTGSEVTPFAVITDKENNIKYPLADYELTPDVAIVD
AQYVTTVPAHITADTGMDVLTHAIESYVSVMASDYTRGLSIRAIELVFENLRESVLTGDPDAREKMHNASALAGMAFANA
FLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNALKPKKHALFPRYESFRADEDYARISRIIGFPAATTEEGVKSLVD
EIIKLGKDVGIDMSLKGQNVAKKDLDAVVDTLADRAFMDQCTTANPKQPLVSELKEIYLEAYKGV

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=386, Percent_Identity=26.4248704663212, Blast_Score=100, Evalue=7e-21,
Organism=Escherichia coli, GI1787493, Length=872, Percent_Identity=48.6238532110092, Blast_Score=801, Evalue=0.0,
Organism=Escherichia coli, GI1789163, Length=387, Percent_Identity=35.9173126614987, Blast_Score=215, Evalue=1e-56,
Organism=Escherichia coli, GI48994951, Length=387, Percent_Identity=33.8501291989664, Blast_Score=193, Evalue=4e-50,
Organism=Escherichia coli, GI87082107, Length=425, Percent_Identity=30.5882352941176, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788797, Length=383, Percent_Identity=32.8981723237598, Blast_Score=172, Evalue=1e-43,
Organism=Escherichia coli, GI1789386, Length=325, Percent_Identity=25.2307692307692, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17537053, Length=428, Percent_Identity=24.5327102803738, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321181, Length=418, Percent_Identity=31.5789473684211, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24657991, Length=423, Percent_Identity=26.9503546099291, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 94667; Mature: 94536

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKENAAQEVLEVQKVIDRLADNGQKALKAFESYNQEQVDNIVHAMALAGLDQHMPLAK
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHH
LAVEETGRGLYEDKCIKNIFATEYIWNNIKNNKTVGVINEDVQTGVIEIAEPVGVVAGVT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
PVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSSAAAKVVYDAAIAAGAPEHCIQWVE
CCCCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
KPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKRSV
CCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
NDIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINPEK
HHHHHCCCCCCCCEECCCCEEEECHHHHHHHHHHHCCCCEEEEECCCHHHHHHHEECCCC
GTLNPDVVGKSPAWIANQAGFKVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAANQ
CCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
AEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKACRIIVNAPSAQGGIGDIYNGF
HHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHHCH
IPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADRRNNMQWFKLPPKIFFEKYSTQYLQKM
HCHHEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHH
EGVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAYQVFADVEPDPSDVTVYKGAELMKD
CCCCEEEEEECCCEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCHHHHHH
FKPDTIIALGGGSAMDAAKGMWLFYEHPEASFFGLKQKFLDIRKRTFKYPKLGGKAKFVA
CCCCEEEEECCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IPTTSGTGSEVTPFAVITDKENNIKYPLADYELTPDVAIVDAQYVTTVPAHITADTGMDV
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECHHHEECCCEEECCCCHHH
LTHAIESYVSVMASDYTRGLSIRAIELVFENLRESVLTGDPDAREKMHNASALAGMAFAN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNALKPKKHALFPRYESFRADEDYAR
HHHCCCHHHHHHCCCCEECCCCCCCEEECCHHHHHCCCCCCHHCCCCCHHHCCCCHHHHH
ISRIIGFPAATTEEGVKSLVDEIIKLGKDVGIDMSLKGQNVAKKDLDAVVDTLADRAFMD
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
QCTTANPKQPLVSELKEIYLEAYKGV
HHCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIKENAAQEVLEVQKVIDRLADNGQKALKAFESYNQEQVDNIVHAMALAGLDQHMPLAK
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHH
LAVEETGRGLYEDKCIKNIFATEYIWNNIKNNKTVGVINEDVQTGVIEIAEPVGVVAGVT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
PVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSSAAAKVVYDAAIAAGAPEHCIQWVE
CCCCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
KPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKRSV
CCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
NDIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINPEK
HHHHHCCCCCCCCEECCCCEEEECHHHHHHHHHHHCCCCEEEEECCCHHHHHHHEECCCC
GTLNPDVVGKSPAWIANQAGFKVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAANQ
CCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
AEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKACRIIVNAPSAQGGIGDIYNGF
HHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHHCH
IPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADRRNNMQWFKLPPKIFFEKYSTQYLQKM
HCHHEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHH
EGVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAYQVFADVEPDPSDVTVYKGAELMKD
CCCCEEEEEECCCEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCHHHHHH
FKPDTIIALGGGSAMDAAKGMWLFYEHPEASFFGLKQKFLDIRKRTFKYPKLGGKAKFVA
CCCCEEEEECCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IPTTSGTGSEVTPFAVITDKENNIKYPLADYELTPDVAIVDAQYVTTVPAHITADTGMDV
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECHHHEECCCEEECCCCHHH
LTHAIESYVSVMASDYTRGLSIRAIELVFENLRESVLTGDPDAREKMHNASALAGMAFAN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AFLGINHSLAHKIGPEFHIPHGRANAILMPHVIRYNALKPKKHALFPRYESFRADEDYAR
HHHCCCHHHHHHCCCCEECCCCCCCEEECCHHHHHCCCCCCHHCCCCCHHHCCCCHHHHH
ISRIIGFPAATTEEGVKSLVDEIIKLGKDVGIDMSLKGQNVAKKDLDAVVDTLADRAFMD
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
QCTTANPKQPLVSELKEIYLEAYKGV
HHCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]