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The map label for this gene is purR [H]

Identifier: 226223648

GI number: 226223648

Start: 1059628

End: 1060656

Strand: Reverse

Name: purR [H]

Synonym: Lm4b_01050

Alternate gene names: 226223648

Gene position: 1060656-1059628 (Counterclockwise)

Preceding gene: 226223655

Following gene: 226223646

Centisome position: 36.41

GC content: 36.25

Gene sequence:

>1029_bases
ATGAAGCTGGAAGATATTGCACGATTAGCAAACGTTTCGAAATCGGCCGTTTCCCTTGCGCTGAATGGAAAAGCTGGCGT
AAGTGAAGAAACCCGTTTACATATTCTGCAAATTGTCGAGGAAAATAATTACGTCCCGTTACGTAATACCAATAAAAAAA
TGAAAAAGAAATCAACCATTCGTTTTATCGCATGCAAAAGTCCTAACTTGATTACCGAACAATATCAGAACTTACCTTTT
TTCAGTGAGCTACTTAGCTATTTATCTGCTGAAATCGCTAGTTACCCATATGATTTGGTTATCTCTACCTTCGATGCAAC
AACCATATTAGAAGAACTTGCTGAAGCAGAAAAAGAGCAACCAACAGCAGGCCTGATTTTACTTGGCACTAATTTAAACG
CTGAAGAAATTAACCGAATACAACAGGAATATCCTAAAATCGTTGTCTTAGATACACACCATCCACATATCTCAGCCAAC
TTTGTCTCTATTAATAATTTTCTCGGTGGATATAAAGCAGCTGACTACTTTTTACAACTAGCACATACAAAAATTGGTTA
TGTGATGGGGGTACCACGCATTAAAAATTTTGATGAGCGGAAAAGTGGCTTTTTTGCTCGTTTAGAAGAAGCCGATATTT
CAGTCCCTGAACAACATGTCTTTCATTTACCAGCAATGCAAATCCAAGAGGAAGTTTCGGTAAAAGAAGCCATTGCTGAA
TTGCAAGATTTACCCTCTGCTCTTTTTTGCGAAAATGATTACATGGCTATTAGCATGATTAAAATGCTACAAAGTCTATC
TATCAAAGTCCCTGAACAAGTTTCCGTATTAGGTTTTGACAATATTAATGAAAGTAAAGTAATTACACCAGAACTAACCA
CTGTTCATGTTAAAAAACGTGATATTGCTGAACAAACACTTGCCCTTTTGTCCAAACAAATTAAAGCTGATACGAATTTT
GAAACCAGACAAATCCAAGTGAATACAAGCTTAATCGAAAGAACTAGCTGTGTTCCGTTCAAAAAATAA

Upstream 100 bases:

>100_bases
CGAGAGGTTTAGAAGACTGAAGGTATGCTATTTTTTGTTTTGATTTACTAAACATGATATACTAAATAAGATAAATTTAG
TAAACAAGGAGAAATGAATA

Downstream 100 bases:

>100_bases
CCAAAAAAGCCTGTGCTCTATTTTTGAGCACAGGCTTTTTTGATATTTTAAATTAATTTTAAATGTTCTAAAGCTTTATA
AACCCCATCTTCATCCACAT

Product: transcriptional regulator, LacI family

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKLEDIARLANVSKSAVSLALNGKAGVSEETRLHILQIVEENNYVPLRNTNKKMKKKSTIRFIACKSPNLITEQYQNLPF
FSELLSYLSAEIASYPYDLVISTFDATTILEELAEAEKEQPTAGLILLGTNLNAEEINRIQQEYPKIVVLDTHHPHISAN
FVSINNFLGGYKAADYFLQLAHTKIGYVMGVPRIKNFDERKSGFFARLEEADISVPEQHVFHLPAMQIQEEVSVKEAIAE
LQDLPSALFCENDYMAISMIKMLQSLSIKVPEQVSVLGFDNINESKVITPELTTVHVKKRDIAEQTLALLSKQIKADTNF
ETRQIQVNTSLIERTSCVPFKK

Sequences:

>Translated_342_residues
MKLEDIARLANVSKSAVSLALNGKAGVSEETRLHILQIVEENNYVPLRNTNKKMKKKSTIRFIACKSPNLITEQYQNLPF
FSELLSYLSAEIASYPYDLVISTFDATTILEELAEAEKEQPTAGLILLGTNLNAEEINRIQQEYPKIVVLDTHHPHISAN
FVSINNFLGGYKAADYFLQLAHTKIGYVMGVPRIKNFDERKSGFFARLEEADISVPEQHVFHLPAMQIQEEVSVKEAIAE
LQDLPSALFCENDYMAISMIKMLQSLSIKVPEQVSVLGFDNINESKVITPELTTVHVKKRDIAEQTLALLSKQIKADTNF
ETRQIQVNTSLIERTSCVPFKK
>Mature_342_residues
MKLEDIARLANVSKSAVSLALNGKAGVSEETRLHILQIVEENNYVPLRNTNKKMKKKSTIRFIACKSPNLITEQYQNLPF
FSELLSYLSAEIASYPYDLVISTFDATTILEELAEAEKEQPTAGLILLGTNLNAEEINRIQQEYPKIVVLDTHHPHISAN
FVSINNFLGGYKAADYFLQLAHTKIGYVMGVPRIKNFDERKSGFFARLEEADISVPEQHVFHLPAMQIQEEVSVKEAIAE
LQDLPSALFCENDYMAISMIKMLQSLSIKVPEQVSVLGFDNINESKVITPELTTVHVKKRDIAEQTLALLSKQIKADTNF
ETRQIQVNTSLIERTSCVPFKK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=354, Percent_Identity=26.271186440678, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790194, Length=344, Percent_Identity=25.8720930232558, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790369, Length=327, Percent_Identity=26.2996941896024, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788474, Length=308, Percent_Identity=26.9480519480519, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789202, Length=299, Percent_Identity=24.4147157190635, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787580, Length=325, Percent_Identity=24.6153846153846, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789068, Length=314, Percent_Identity=26.4331210191083, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38532; Mature: 38532

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEDIARLANVSKSAVSLALNGKAGVSEETRLHILQIVEENNYVPLRNTNKKMKKKSTI
CCHHHHHHHHHCCHHHEEEEECCCCCCCCHHHEEEEEEEECCCEECCCCCCHHHHHHCCE
RFIACKSPNLITEQYQNLPFFSELLSYLSAEIASYPYDLVISTFDATTILEELAEAEKEQ
EEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHEEECCCHHHHHHHHHHHHHCC
PTAGLILLGTNLNAEEINRIQQEYPKIVVLDTHHPHISANFVSINNFLGGYKAADYFLQL
CCCCEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCEEEEEEEHHHHCCCHHHHHHHHHH
AHTKIGYVMGVPRIKNFDERKSGFFARLEEADISVPEQHVFHLPAMQIQEEVSVKEAIAE
HHHHHHHHCCCCCCCCCCHHHCCHHEEECCCCCCCCHHHHEECCHHHHHHHHHHHHHHHH
LQDLPSALFCENDYMAISMIKMLQSLSIKVPEQVSVLGFDNINESKVITPELTTVHVKKR
HHHCCHHEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEECCCCEEEEEHHH
DIAEQTLALLSKQIKADTNFETRQIQVNTSLIERTSCVPFKK
HHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLEDIARLANVSKSAVSLALNGKAGVSEETRLHILQIVEENNYVPLRNTNKKMKKKSTI
CCHHHHHHHHHCCHHHEEEEECCCCCCCCHHHEEEEEEEECCCEECCCCCCHHHHHHCCE
RFIACKSPNLITEQYQNLPFFSELLSYLSAEIASYPYDLVISTFDATTILEELAEAEKEQ
EEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHEEECCCHHHHHHHHHHHHHCC
PTAGLILLGTNLNAEEINRIQQEYPKIVVLDTHHPHISANFVSINNFLGGYKAADYFLQL
CCCCEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCEEEEEEEHHHHCCCHHHHHHHHHH
AHTKIGYVMGVPRIKNFDERKSGFFARLEEADISVPEQHVFHLPAMQIQEEVSVKEAIAE
HHHHHHHHCCCCCCCCCCHHHCCHHEEECCCCCCCCHHHHEECCHHHHHHHHHHHHHHHH
LQDLPSALFCENDYMAISMIKMLQSLSIKVPEQVSVLGFDNINESKVITPELTTVHVKKR
HHHCCHHEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEECCCCEEEEEHHH
DIAEQTLALLSKQIKADTNFETRQIQVNTSLIERTSCVPFKK
HHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA