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The map label for this gene is rnjA [H]

Identifier: 226223645

GI number: 226223645

Start: 1056443

End: 1058110

Strand: Reverse

Name: rnjA [H]

Synonym: Lm4b_01047

Alternate gene names: 226223645

Gene position: 1058110-1056443 (Counterclockwise)

Preceding gene: 226223646

Following gene: 226223644

Centisome position: 36.33

GC content: 38.49

Gene sequence:

>1668_bases
ATGAAATTTGTTAAAAATAATGAGACAGCTGTTTTCGCCCTTGGTGGACTAGGCGAAATCGGTAAAAACACATATGGCGT
ACAATTTCAAGACGAAATTATTCTAATTGATGCAGGAATCAAGTTCCCGGAAGACGAGCTTCTTGGTATTGATTATGTTA
TTCCTGACTACAGTTACCTAGTTAAAAACAAAGATAAGATTAAAGGGCTTTTTATCACCCATGGTCATGAAGATCATATC
GGTGGCGTTCCTTACCTACTTCGCGATCTTAATATCCCAATTTACGCTGGGAAACTTGCGAGTGCACTTATCCGCAACAA
ATTAGAAGAACATGGCTTGCTTCGTCAAACCAAAATTTATGAATATGAAGAAGATGACGTTTTTAAATTCAGAAAAACAA
GTATCTCTTTCTTCCGTACAACCCATAGTATTCCTGATACGTATGGTATTGTTGTCAAAACTCCATCTGGTAACATCGTT
CATACTGGTGACTTCAAGTTTGATTTTACTCCAGTTGGCGAACCAGCAAACCTAACAAAAATGGCTGAAATCGGTAAAGA
AGGCGTTCTTTGTTTACTATCTGATAGTACAAATGCGGAAGTACCAAATTTCACCATGAGTGAGCGTGTCGTTGGCGAAA
GTATTAAAAATATTTTCCGCGATGTAGAAGGACGAATTATTTTCGCTACCTTCGCATCTAATATTTACCGTTTACAACAA
GTAGTTGAATCCTCTATCGAAACTGGCCGTAAAATCGCTGTTTTCGGTCGTAGTATGGAATCTGCTATGGAAATCGGTAA
ACAATTAGGCTATATTCAAGCGCCAAAAGATACATTTATTGATGTGCATCAACTTAATAAAACACCCGCTGGAAATGTGA
CTATCCTTTGTACAGGGAGTCAAGGTGAACCAATGGCTGCTCTTTCTAGAATTGCTAATGGTACGCACCGCCAAATCCAA
ATTCAACCAGGTGACACGGTAGTCTTCTCGTCTTCCCCAATCCCTGGTAATACAACGAGTGTAAACCGCACAATCAATTT
ACTATATCAAGCTGGTGCAGAAGTTATCCATGGTAAAGTAAATAATATCCATACTTCTGGTCACGGTGGTCAACAAGAAC
AAAAACTAATGCTTCGTTTGATGAAACCTAAATTCTTTATGCCAATTCATGGTGAATTCAGAATGCAAAAAATCCATGCA
GAGTCTGCTGAAGAATGTGGTGTTCCAGAAGAAAATAGCTTTATTATGGCAAATGGAGATGTACTCGCATTAACAAGTGA
AACAGCTCATGTTGCTGGTAAAATCCATGCAGCAAGCGTATATATTGATGGTAGCGGTATTGGCGATATCGGTAATATTG
TCCTTCGCGATCGTCGTATTCTTTCTGAAGAAGGCCTCGTAATTGTTGTTGTATCCATTGATATGAAAAATTCTCGTGTG
ATGGCAGGACCTGATATTATTTCACGCGGCTTCATTTATATGCGTGAATCCGGTAATCTCATCGGAGAAGCACAAGGCCT
CCTAACGCGACACTTAAATAAAGTAATGGAACAAAAAACAACGCAATGGTCTGAAATCAAAAATGAAATTACAGATACTT
TGCAACCTTTCTTGTACGAAAAAACAAAACGTCGTCCAATGATTTTACCAATAATCATGGAAGTATAG

Upstream 100 bases:

>100_bases
TGAACCTTTCCACATCGAATTTGTCGAAAAAATTAGTGACGCGCACTTAGCTTATGAAAAGGAAAACCCAGACTTCGCGC
TCTGGGAAAAATAAGCCTAT

Downstream 100 bases:

>100_bases
TTTTCAAGACGATAAATGGTTTCAAAATCATTTATCGTTTTTTTGTTGATTTCTCAGAAAGACGATGGTATGTTTAAACA
AACAAAATAGAAAGACGAGG

Product: hypothetical protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKFVKNNETAVFALGGLGEIGKNTYGVQFQDEIILIDAGIKFPEDELLGIDYVIPDYSYLVKNKDKIKGLFITHGHEDHI
GGVPYLLRDLNIPIYAGKLASALIRNKLEEHGLLRQTKIYEYEEDDVFKFRKTSISFFRTTHSIPDTYGIVVKTPSGNIV
HTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNAEVPNFTMSERVVGESIKNIFRDVEGRIIFATFASNIYRLQQ
VVESSIETGRKIAVFGRSMESAMEIGKQLGYIQAPKDTFIDVHQLNKTPAGNVTILCTGSQGEPMAALSRIANGTHRQIQ
IQPGDTVVFSSSPIPGNTTSVNRTINLLYQAGAEVIHGKVNNIHTSGHGGQQEQKLMLRLMKPKFFMPIHGEFRMQKIHA
ESAEECGVPEENSFIMANGDVLALTSETAHVAGKIHAASVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMKNSRV
MAGPDIISRGFIYMRESGNLIGEAQGLLTRHLNKVMEQKTTQWSEIKNEITDTLQPFLYEKTKRRPMILPIIMEV

Sequences:

>Translated_555_residues
MKFVKNNETAVFALGGLGEIGKNTYGVQFQDEIILIDAGIKFPEDELLGIDYVIPDYSYLVKNKDKIKGLFITHGHEDHI
GGVPYLLRDLNIPIYAGKLASALIRNKLEEHGLLRQTKIYEYEEDDVFKFRKTSISFFRTTHSIPDTYGIVVKTPSGNIV
HTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNAEVPNFTMSERVVGESIKNIFRDVEGRIIFATFASNIYRLQQ
VVESSIETGRKIAVFGRSMESAMEIGKQLGYIQAPKDTFIDVHQLNKTPAGNVTILCTGSQGEPMAALSRIANGTHRQIQ
IQPGDTVVFSSSPIPGNTTSVNRTINLLYQAGAEVIHGKVNNIHTSGHGGQQEQKLMLRLMKPKFFMPIHGEFRMQKIHA
ESAEECGVPEENSFIMANGDVLALTSETAHVAGKIHAASVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMKNSRV
MAGPDIISRGFIYMRESGNLIGEAQGLLTRHLNKVMEQKTTQWSEIKNEITDTLQPFLYEKTKRRPMILPIIMEV
>Mature_555_residues
MKFVKNNETAVFALGGLGEIGKNTYGVQFQDEIILIDAGIKFPEDELLGIDYVIPDYSYLVKNKDKIKGLFITHGHEDHI
GGVPYLLRDLNIPIYAGKLASALIRNKLEEHGLLRQTKIYEYEEDDVFKFRKTSISFFRTTHSIPDTYGIVVKTPSGNIV
HTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNAEVPNFTMSERVVGESIKNIFRDVEGRIIFATFASNIYRLQQ
VVESSIETGRKIAVFGRSMESAMEIGKQLGYIQAPKDTFIDVHQLNKTPAGNVTILCTGSQGEPMAALSRIANGTHRQIQ
IQPGDTVVFSSSPIPGNTTSVNRTINLLYQAGAEVIHGKVNNIHTSGHGGQQEQKLMLRLMKPKFFMPIHGEFRMQKIHA
ESAEECGVPEENSFIMANGDVLALTSETAHVAGKIHAASVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMKNSRV
MAGPDIISRGFIYMRESGNLIGEAQGLLTRHLNKVMEQKTTQWSEIKNEITDTLQPFLYEKTKRRPMILPIIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61561; Mature: 61561

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVKNNETAVFALGGLGEIGKNTYGVQFQDEIILIDAGIKFPEDELLGIDYVIPDYSYL
CCCEECCCEEEEEECCCCCCCCCCCCEEEECCEEEEECCCCCCCHHEEEEEEECCCCCEE
VKNKDKIKGLFITHGHEDHIGGVPYLLRDLNIPIYAGKLASALIRNKLEEHGLLRQTKIY
CCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCEEHHEEE
EYEEDDVFKFRKTSISFFRTTHSIPDTYGIVVKTPSGNIVHTGDFKFDFTPVGEPANLTK
ECCCCCCEEHHHHHEEEEEEECCCCCCEEEEEECCCCCEEECCCCEEEEECCCCCCCHHH
MAEIGKEGVLCLLSDSTNAEVPNFTMSERVVGESIKNIFRDVEGRIIFATFASNIYRLQQ
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHH
VVESSIETGRKIAVFGRSMESAMEIGKQLGYIQAPKDTFIDVHQLNKTPAGNVTILCTGS
HHHHHHHCCCEEEEECCCHHHHHHHHHHCCEEECCCCCEEEHHHCCCCCCCCEEEEEECC
QGEPMAALSRIANGTHRQIQIQPGDTVVFSSSPIPGNTTSVNRTINLLYQAGAEVIHGKV
CCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHCHHHEECCC
NNIHTSGHGGQQEQKLMLRLMKPKFFMPIHGEFRMQKIHAESAEECGVPEENSFIMANGD
CEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEHHCCCHHHCCCCCCCCEEEECCC
VLALTSETAHVAGKIHAASVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMKNSRV
EEEEECCCHHHEEEEEEEEEEECCCCCCCHHHHEECCCHHHCCCCEEEEEEEEECCCCEE
MAGPDIISRGFIYMRESGNLIGEAQGLLTRHLNKVMEQKTTQWSEIKNEITDTLQPFLYE
EECCCHHHCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
KTKRRPMILPIIMEV
HHCCCCEEEEEEECC
>Mature Secondary Structure
MKFVKNNETAVFALGGLGEIGKNTYGVQFQDEIILIDAGIKFPEDELLGIDYVIPDYSYL
CCCEECCCEEEEEECCCCCCCCCCCCEEEECCEEEEECCCCCCCHHEEEEEEECCCCCEE
VKNKDKIKGLFITHGHEDHIGGVPYLLRDLNIPIYAGKLASALIRNKLEEHGLLRQTKIY
CCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCEEHHEEE
EYEEDDVFKFRKTSISFFRTTHSIPDTYGIVVKTPSGNIVHTGDFKFDFTPVGEPANLTK
ECCCCCCEEHHHHHEEEEEEECCCCCCEEEEEECCCCCEEECCCCEEEEECCCCCCCHHH
MAEIGKEGVLCLLSDSTNAEVPNFTMSERVVGESIKNIFRDVEGRIIFATFASNIYRLQQ
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHH
VVESSIETGRKIAVFGRSMESAMEIGKQLGYIQAPKDTFIDVHQLNKTPAGNVTILCTGS
HHHHHHHCCCEEEEECCCHHHHHHHHHHCCEEECCCCCEEEHHHCCCCCCCCEEEEEECC
QGEPMAALSRIANGTHRQIQIQPGDTVVFSSSPIPGNTTSVNRTINLLYQAGAEVIHGKV
CCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHCHHHEECCC
NNIHTSGHGGQQEQKLMLRLMKPKFFMPIHGEFRMQKIHAESAEECGVPEENSFIMANGD
CEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEHHCCCHHHCCCCCCCCEEEECCC
VLALTSETAHVAGKIHAASVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMKNSRV
EEEEECCCHHHEEEEEEEEEEECCCCCCCHHHHEECCCHHHCCCCEEEEEEEEECCCCEE
MAGPDIISRGFIYMRESGNLIGEAQGLLTRHLNKVMEQKTTQWSEIKNEITDTLQPFLYE
EECCCHHHCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
KTKRRPMILPIIMEV
HHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]